MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/m_MTBLS14325_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/m_MTBLS14325_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/s_MTBLS14325.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/a_MTBLS14325_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/a_MTBLS14325_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/QC1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/QC3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T1_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/QC2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/QC4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_NC_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/QC2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/QC4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T2_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_T1_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/Ser_T2_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/Ser_NC_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/POS/QC1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325/FILES/RAW_FILES/NEG/QC3.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14325<p>Metabolite identification was performed based on the Personalbio PSNGM (PerSonalbio Next-Generation Metabolomics Database), which includes an in-house built database of standards, the mzCloud library (https://www.mzcloud.org/), LIPID MAPS (https://www.lipidmaps.org/), HMDB (https://hmdb.ca/), MoNA (https://mona.fiehnlab.ucdavis.edu/), NIST_2020_MSMS, and an AI-predicted MS/MS spectral library. The main parameters for library searching were as follows: MS1 tolerance for identification 0.01, MS2 tolerance for identification 0.05, Smoothing level 3, Minimum peak height 10000, Minimum peak width 5, Mass slice width 0.05, Identification score cut-off 70.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>An ACQUITY UPLC HSS T3 column (100 A, 1.8 µm, 2.1 mm x 100 mm) was used at a flow rate of 0.4 mL/min, a column temperature of 40 °C, an autosampler temperature of 8 °C, and an injection volume of 2 μL. The mobile phases for both positive and negative ion modes were as follows: mobile phase A was 0.1% formic acid in water, and mobile phase B was acetonitrile containing 0.1% formic acid.</p>Untargeted metabolomic analysis of lamb serum.Duo KeaiPeking Universityserummass spectrometry assay<p>A volume of 50 μL of serum sample was transferred to a 2 mL centrifuge tube, and 200 μL of pre-chilled methanol/acetonitrile solution (1:1, v/v) was added. After thorough mixing, the mixture was stored at 20°C for 30 minutes. Subsequently, centrifugation was performed at 12,000 rpm and 4°C for 10 minutes. A 200 μL aliquot of the supernatant was collected, mixed with 150 μL of 50% methanol for reconstitution, and centrifuged again under the same conditions. The resulting supernatant was filtered through a 0.22 μm membrane, and the filtrate was transferred to an injection vial for analysis. To evaluate instrument stability and data reliability, a quality control (QC) sample was prepared by pooling 20 μL of filtrate from each individual sample.</p>Ovis arieshttps://www.ebi.ac.uk/metabolights/MTBLS14325Yang Gao. Tianjin Agricultural University. gy13512866631@163.com.liang hong. Tianjin Agricultural University. hongl0626@163.com.<p>The acquired .raw data were imported into the MS-DIAL software (version 4.9.221218). Peak extraction, alignment, filtering, and metabolite identification were performed using this software. Peaks not detected in more than 50% of the QC samples were filtered out. For the remaining missing peaks, missing values were filled using the software’s gap-filling algorithm, followed by normalization.</p>Groupkeaiduoduo998@126.com<p>Lamb serum collected at Tianjin Agricultural University</p>MetabolomicsMetabolomicsOvis aries70-1000untargeted analysisThermo Scientific Vanquish Flex UHPLC SystemThermo Scientific Orbitrap Exploris 120Untargeted metabolomic analysislambserumLCMSMetabolomicsOvis aries70-1000untargeted analysisThermo Scientific Vanquish Flex UHPLC SystemThermo Scientific Orbitrap Exploris 120Untargeted metabolomic analysislambserumLCMS<p>The Thermo Orbitrap Exploris 120 mass spectrometer, controlled by Xcalibur software (version 4.7, Thermo Fisher Scientific), was used for DDA (data-dependent acquisition) mass spectrometry data acquisition in positive and negative ion modes, respectively. A HESI (heated electrospray ionization) source was employed with spray voltages of +3.5 kV and –3.0 kV, sheath gas at 40 arb, auxiliary gas at 10 arb, capillary temperature at 320 °C, and auxiliary gas temperature at 300 °C. The MS1 resolution was set to 60,000, with a scan range of 70–1000 m/z, AGC target set to Standard, and maximum injection time (Max IT) of 100 ms. The top 4 most intense precursor ions were selected for MS/MS fragmentation with a dynamic exclusion time of 4 s. The MS2 resolution was 15,000, HCD collision energy was set to 30%, AGC target was Standard, and Max IT was set to Auto.</p>CORTEXOLONEDEOXYCARNITINEAminohippuric acidTaurine1,3-Diphenyl-2-propanoneLysoPC(20:3(8Z,11Z,14Z)/0:0)13-KODEFluoroacetic acidBicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-3-(phenylmethylene)-BETAINEN6-METHYLLYSINEphenylethanolamine2-Nitrofurangamma-Aminobutyric acidD-(-)-MannitolLumirubin12(13)Ep-9-KODEL-HistidinePhe-TrpProlinamideEugenol,98% (stabilized with TBC)Myristoleic acidAMINOADIPATE1-MethylpyrrolidineN-(1-Deoxy-1-fructosyl)tyrosineN-(1-Deoxy-1-fructosyl)isoleucineButyl benzoate18-HETEPC(16:0/0:0)N2-MethylguanosineL-ProlineUreaLysoPC(22:4(7Z,10Z,13Z,16Z)/0:0)LysoPC(20:4(8Z,11Z,14Z,17Z)/0:0)12E, 14E-Octadecadienoic acidKYNURENATE1-Dodecanesulfonic acid, 1-hydroxy-3-oxo-N1,N11-Bis(ethyl)norspermine1-MethylguanosineESTRADIOL-17ALPHACalystegine A6SuccinimideD-beta-homoserineCordeauxione2-HydroxyisovalerylcarnitineRicinoleic acidN,N-Didemethyl orphenadrine5,6-epoxy-8Z,11Z,14Z-eicosatrienoic acidProline-hydroxyprolineL-PalmitoylcarnitineHederageninArlatinUreidopropionic acidPalmitelaidic acidLysoPC(20:5(5Z,8Z,11Z,14Z,17Z)/0:0)Linoleamide1-stearoyl-lysophosphatidylcholine(+/-)-RibalineMonoisobutyl phthalic acidL-TheanineNiacinamideCORTICOSTERONE1-PiperidinecarboxaldehydeMyristoyl-carnitine(+)-2,3-Dihydro-3-methyl-1H-pyrroleKojic acidMagnoflorineCiramadolGLYCOCHOLIC ACID5-HydroxytryptopholPhenylacetylglycineN-AcetylvanilalanineN1,N8-Diacetylspermidine17-ODYALysoPC(14:0/0:0)Cycloleucine5-HYDROXYINDOLEACETATEL-CARNITINELysoPE(0:0/18:2(9Z,12Z))Pentaethylene glycol2,7-Diamino-7-iminoheptanoic acid2-DecenoylcarnitineLeucylproline1,5-dimethyl-1-vinyl-4-hexenyl acetateOleamide4'-O-MethylequolGalbanic acidL-ValineUraciltaurocholic acid7-Epi-12-hydroxyjasmonic acidPhloroglucinolL-KynurenineGlutamylphenylalanineD-FucoseCreatineStearoyl-L-carnitinePiperidin-2-One6-Hydroxy-1H-indole-3-acetamideglutaralFructosylvalineDiisodecyl phthalateACETYL-CARNITINELAUROYLCARNITINEL(+)-Ornithine1-Naphthyl isocyanateCiprostenePotassium acetateBiliverdinalpha-IononeImidazoloneN,N-Diethyl-2-methylbenzamidePicolinoylglycine1-oleoyl-sn-glycero-3-phosphoethanolamine3-Indolepropionic acidPC(P-16:0/22:0)DiethylamineN-Butyrylglycine2-Aminoisobutyric AcidLysoPC(20:3(5Z,8Z,11Z)/0:0)(+)-1,2-Epoxyneomenthyl acetate1,2,4-Oxadiazole1-(2,4,6-Trimethylphenoxy)propan-2-amineD-Erythro-sphingosine 1-phosphateTrp-PhePhytoporphyrinGLYCOCHENODEOXYCHOLATETetrahydropyridineBenzamide5,9:6,9-Diepoxy-3-megastigmeneGlutaric anhydride2-Ethylpropanedioylcarnitine2-ImidazolidoneCITRULLINE3-Aminoazetidin-2-oneFuranone A2,3-Diethyl-6-hexoxycarbonylbenzoic acidL-alpha-GlycerylphosphorylcholineMethyl palmitateHomovanillyl alcoholDECANOYLCARNITINE1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid[7]-GingerolL-Glutamic acidURSOCHOLIC ACIDL-Thyroxine7alpha,25-DihydroxycholesterolN,N-DimethylglycineGALACTITOL3-Methylglutaconic acidCalcium PantothenateURSODEOXYCHOLATE2-Amino-3-methyl-1-pyrrolidin-1-yl-butan-1-oneLysoPC(0:0/20:4(5Z,8Z,11Z,14Z))1-Oleoyl-sn-glycero-3-phosphocholine3-HYDROXY-2-METHYL-4-PYRONELinoleic acidCilostamide2'-OxoquinidinoneSaxitoxinPalmitoleic acidC3-CarnitineD-ribo-PhytosphingosineIsohyodeoxycholic acidS-(2-carboxypropyl)-Cysteamine2,6-diamino-4-hydroxy-5-formamidopyrimidineoctopamineAminoacetone1-MethylinosineDodecanamideTriethylene glycolBicinecis-5-TetradecenoylcarnitineTropic acidTsibulin 1L-Isoleucyl-L-alanine4-HydroxybenzoylcholineLysoPE(20:4(5Z,8Z,11Z,14Z)/0:0)12-oxo Phytodienoic AcidlenticinMethylhexahydrophthalic anhydrideL-METHIONINE3,4-MethylenedioxycinnamaldehydeSedanolideAlpha-Eleostearic acid2,5-Dihydropyrimidine(2R,5S)-2-(6-Aminopurin-9-yl)-5-(methylsulfanylmethyl)oxolane-3,4-diolN,N-dimethylarginine5-Hydroxy-4-pentenoic acid d-lactoneLinoleyl-carnitine3-FormylindoleTheophyllinetrimethylglycinepantothenic acidbenzyl alcoholPlatelet-activating factor4-Tetradecanamidobenzylphosphonic acidAcetohydroxamic acid3-OxononanoylcarnitineLysoPC(16:1(9Z)/0:0)Leucyl-leucineTrilostaneSesamolGuanineResorufinEchinocystic acidLevoglucosanOleoyl ethanolamideOctadecanamideDichloromethylene7,8-Diaminononanoic acidFructoselysineL-(+)-LeucineFormamide, N-[(1Z)-2-(4-methoxyphenyl)ethenyl]-CreatinineKYNURENINEAB-PINACADL-NorleucineAspartyl-L-prolinePHENETHYLAMINEButyronitrileval-phe(2-hydroxyethyl)trimethylammonium(Z)-5-Chloro-3-((3,5-dimethyl-1H-pyrrol-2-yl)methylene)indolin-2-oneloliolideRimantadineL-ARGININE2-ChloroanilineTris(hydroxymethyl)aminomethaneL-HexanoylcarnitineDibenzylamineGlutamylvaline3,5-Dihydroxy-4-methoxybenzaldehyde8-[(2R,3S)-3-(8-Hydroxyoctyl)oxiran-2-yl]octanoylcarnitineArachidoyl Ethanolamide2-(1H-indol-3-yl)acetic acidDibutyl phthalate1-Methyl-4-(1-methyl-2-propenyl)-benzeneTauroursodeoxycholic acidCycloserine5,8,11,14-Hexadecatetraenoicacid, 16-[(2R,3S)-3-ethyl-2-oxiranyl]-, (5Z,8Z,11Z,14Z)-rel-NitrosonornicotineCYTIDINEButyryl-carnitinePivaloylcarnitine1,N6-Etheno-adenineStearic acidDimethyl carbonate4-Quinolinamine, N-hydroxy-, 1-oxideErucamide3,5-Di-tert-butyl-4-hydroxybenzaldehydeEremopetasinorone ACHOLATE1-Oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine(-)-Ecgonine methyl ester2,2,6,6-Tetramethylpiperidin-1-olLysoPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)quininealpha-ketoadipic acidSuccinamideL-Lysine2-Hydroxy-5-octenoylcarnitineTaurochenodeoxycholic acid(1S,3R)-ACPDLysoPC(22:5(4Z,7Z,10Z,13Z,16Z)/0:0)Trimethylamine N-oxidePhthalic anhydride1,3-Dicyclohexylurea3-Hepteneoylglycine7-HexadecenoylcarnitineTrans-2, 3, 4-Trimethoxycinnamate2-Oxahexacyclo[15.3.1.03,20.06,19.09,18.010,15]henicosane-16,21-diolELAIDIC ACIDAllopurinol ribosidePalmitamide1-[(9Z)-octadecenoyl]-2-tetradecanoyl-sn-glycero-3-phosphocholine(-)-ErythromycinL-Carnosine1-METHYL-L-HISTIDINEPalmitic AcidSEROTONINPyrrolidineLysoPC(22:5(7Z,10Z,13Z,16Z,19Z)/0:0)Piperidine5-Pentyl-2-furanheptanoic acid13-Oxo-9,11-octadecadienoic acid1-PyrrolidinecarboxaldehydeNICOTINATECadaverine7-EthoxycoumarinGlycodeoxycholic AcidCAPRYLOYL GLYCINEOleoyl-carnitineCeramide (d18:1/16:0)MesalazinePC(P-16:0/20:0)PhytuberolSarcosine13-Oxo-9,11-tridecadienoic acidLysoPC(18:3(9Z,12Z,15Z)/0:0)9,10-Epoxy-12-octadecenoic acidMALEIMIDETaurodeoxycholic Acid (sodium salt)1-Hydroxybenzotriazole13,14-Dihydro-15-keto Prostaglandin E2OxazinefalseUntargeted metabolomic analysis of lamb serumUsing untargeted metabolomics to investigate the effects of different supplementation routes on serum metabolites in 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