MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/m_MTBLS14342_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/m_MTBLS14342_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/s_MTBLS14342.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/a_MTBLS14342_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342/a_MTBLS14342_LC-MS_positive_reverse-phase.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14342<p> The raw data collected using MassLynx V4.2 is processed by Progenesis QI software for peak extraction, peak alignment and other data processing operations, based on the Progenesis QI software online METLIN database and Biomark’s self-built library for identification.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>The column used is purchased from Waters Acquity UPLC HSS T3 column (1.8um 2.1*100mm). Positive ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile. Negative ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile. Injection volume 2μL.</p>NHE gene knockdown in the Chinese mitten crab.Yancheng Teachers UniversityAobo Panggillmass spectrometry assay<p>Frozen gill samples were homogenized in ice cold methanol, centrifuged, and the supernatant was dried and reconstituted in methanol/water before filtration and HPLC injection. Mixed extract from all samples, injected regularly to monitor instrument stability.</p>Eriocheir sinensishttps://www.ebi.ac.uk/metabolights/MTBLS14342Aobo Pang. Yancheng Teachers University. public_jlcbe@163.com.<p> The raw data collected using MassLynx V4.2 is processed by Progenesis QI software for peak extraction, peak alignment and other data processing operations, based on the Progenesis QI software online METLIN database and Biomark’s self-built library for identification.</p>Treatmentpublic_jlcbe@163.com<p>The samples were obtained from NHE gene knockout crabs (KD) and non knockout control crabs (CK), each exposed to three salinity stresses (0, 15, and 30 ppt). At a designated time point (day 7) after the start of salinity exposure, the crabs were quickly euthanized, the carapace was opened, and the gill tissue was rapidly dissected using prechilled scissors and forceps. The gills were briefly rinsed with cooled saline (5 seconds), blotted dry with absorbent paper, immediately placed into cryovials, and snap frozen in liquid nitrogen.</p>MetabolomicsMetabolomicsgillWaters ACQUITY UPLC I-Class PLUS SystemWaters Xevo G2-XS QTofuntargeted analysisMassLynxEriocheir sinensisWaters (China)Progenesis QIexperimental sampleMetabolomicsgillWaters ACQUITY UPLC I-Class PLUS SystemWaters Xevo G2-XS QTofuntargeted analysisMassLynxEriocheir sinensisWaters (China)Progenesis QIexperimental sample<p>Waters Xevo G2-XS QTOF high resolution mass spectrometer can collect primary and secondary mass spectrometry data in MSe mode under the control of the acquisition software (MassLynx V4.2, Waters). In each data acquisition cycle, dual-channel data acquisition can be performed on both low collision energy and high collision energy at the same time. The low collision energy is off, the high collision energy range is 10~40V, and the scanning frequency is 0.2 seconds for a mass spectrum. The parameters of the ESI ion source are as follows: Capillary voltage: 2500V (positive ion mode) or -2000V (negative ion mode); cone voltage: 30V; ion source temperature: 100°C; desolvent gas temperature 500°C; backflush gas flow rate: 50L/ h; Desolventizing gas flow rate: 800L/h.</p>falseNHE gene knockdown in the Chinese mitten crabMetabolomic analysis of anadromous juvenile crabs at different salinities following NHE gene knockdown2026-04-222026-04-22MTBLS14342