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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14352/m_MTBLS14352_LC-MS_alternating_normal-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14352/s_MTBLS14352.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14352/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14352/a_MTBLS14352_LC-MS_alternating_normal-phase.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14352Raw data were converted to the open mzXML format using ProteoWizard. Feature identification was performed with a custom in-house R program built on XCMS (v3.12.0 kernel) for peak detection, extraction, alignment, and integration. For metabolite annotation, acquired MS/MS spectra were matched against the in-house MS/MS spectral database BiotreeDB (V2.1) by combining metabolite retention time, precursor m/z (mass error < 10 ppm), and MS/MS fragmentation patterns. The algorithm score cutoff for annotation was set at 0.3. Metabolite identification confidence was classified according to the 2021 Nature Methods reporting framework (Levels A–D).MetaboLightsPublicLiquid Chromatography MS - alternating - normal-phaseLC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a UPLC BEH Amide column (2.1 mm × 100 mm, 1.7 μm) coupled to Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo). The mobile phase consisted of 25 mmol/L ammonium acetate and 25 mmol/L ammonium hydroxide in water (pH = 9.75) (A) and acetonitrile (B). The auto-sampler temperature was 4 °C, and the injection volume was 2 μL.Metabolome of Streptomyces sp. HU2014.Xinjiang academy of agricultural sciencesLinfeng Hunot applicablemass spectrometry assay100 μL of sample was transferred to an EP tube. After the addition of 400 μL of extract solution (acetonitrile : methanol = 1:1, containing isotopically-labelled internal standard mixture), the samples were vortexed for 30 s, sonicated for 10 min in ice-water bath, and incubated for 1 h at -40 °C to precipitate proteins. Then the sample was centrifuged at 12 000 rpm (RCF = 13 800 (×g), R = 8.6 cm) for 15 min at 4 °C. The resulting supernatant was transferred to a fresh glass vial for analysis. The quality control (QC) sample was prepared by mixing an equal aliquot of the supernatants from all of the samples.Streptomyces sp. HU2014

HU2014 was inoculated into an optimized medium containing dextrin (39.59 g/L), yeast extract (8.52 g/L), and KNO3 (2.49 g/L), with the initial pH adjusted to 7.0–7.3, and fermented at 25°C and 120 rpm for 15 days. Fermentation supernatants were collected at four time points: day 0 (C0/control), day 5 (T1), day 10 (T2), and day 15 (T3), with six biological replicates for each time point. Samples were filtered through 0.2 µm membranes, immediately snap-frozen in liquid nitrogen, and stored for subsequent analysis.

https://www.ebi.ac.uk/metabolights/MTBLS14352Hongxia Zhu.Linfeng Hu. Xinjiang Academy of Agricultural Sciences. wood9818@sina.com.The acquired raw mass spectrometry data were converted from the vendor-specific format to the open mzXML format using ProteoWizard. Feature detection, including peak picking, extraction, alignment, and integration, was carried out with a custom in-house R package based on XCMS. Metabolite annotation was achieved by searching the acquired MS/MS spectra against an in-house MS/MS spectral database (BiotreeDB V2.1), applying an algorithm score cutoff of 0.3 for confident assignments.Fermentation timewood9818@sina.comHU2014 was inoculated into an optimized medium containing dextrin (39.59 g/L), yeast extract (8.52 g/L), and KNO3 (2.49 g/L), with the initial pH adjusted to 7.0–7.3, and fermented at 25°C and 120 rpm for 15 days. Fermentation supernatants were collected at four time points: day 0 (C0/control), day 5 (T1), day 10 (T2), and day 15 (T3), with six biological replicates for each time point. Samples were filtered through 0.2 µm membranes, immediately snap-frozen in liquid nitrogen, and stored for subsequent analysis.Metabolomicsultra-performance liquid chromatography-mass spectrometryThermo Scientific Vanquish UHPLC SystemMulti-omics studyMetabolomicsnot applicableuntargeted analysisThermo Scientific Orbitrap Exploris 120Streptomyces sp. HU2014experimental blankuntargeted metabolite profilingThermo Scientific Vanquish UHPLC Systemultra-performance liquid chromatography-mass spectrometryMulti-omics studyMetabolomicsnot applicableuntargeted analysisThermo Scientific Orbitrap Exploris 120Streptomyces sp. HU2014experimental blankuntargeted metabolite profilingThe Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS spectra on information-dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). In this mode, the acquisition software continuously evaluates the full scan MS spectrum. The ESI source conditions were set as following: sheath gas flow rate as 50 Arb, Aux gas flow rate as 15 Arb, capillary temperature 320 °C, full MS resolution as 60000, MS/MS resolution as 15000, collision energy as 10/30/60 in NCE mode, spray Voltage as 3.8 kV (positive) or −3.4 kV (negative), respectively.falseMetabolome of Streptomyces sp. HU2014HU2014 was inoculated into an optimized medium containing dextrin (39.59 g/L), yeast extract (8.52 g/L), and KNO3 (2.49 g/L), with the initial pH adjusted to 7.0–7.3, and fermented at 25°C and 120 rpm for 15 days. Fermentation supernatants were collected at four time points: day 0 (C0/control), day 5 (T1), day 10 (T2), and day 15 (T3), with six biological replicates for each time point. Samples were filtered through 0.2 µm membranes, immediately snap-frozen in liquid nitrogen, and stored for subsequent analysis.2026-04-222026-04-22MTBLS14352