{"database":"MetaboLights","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/m_MTBLS14366_LC-MS_negative_reverse-phase_v2_maf.tsv","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/m_MTBLS14366_LC-MS_positive_reverse-phase_v2_maf.tsv"],"Txt":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/s_MTBLS14366.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/a_MTBLS14366_LC-MS_negative_reverse-phase.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/a_MTBLS14366_LC-MS_positive_reverse-phase.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/i_Investigation.txt"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"ftp_download_link":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366"],"metabolite_identification_protocol":["<p>Metabolite annotation was performed using Progenesis QI v3.0 by matching MS and MS/MS spectra against public and in-house databases. The main databases included HMDB, METLIN, and the Majorbio in-house database. The MS mass error threshold was set to less than 10 ppm, and metabolite identification was further supported by MS/MS fragmentation matching scores.</p>"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["<p>LC separation was performed using a Thermo Scientific Vanquish Horizon UHPLC system. Chromatographic separation was achieved on an ACQUITY UPLC HSS T3 column, 1.8 μm, 2.1 × 100 mm, Waters. Mobile phase A consisted of 95% water and 5% acetonitrile with 0.1% formic acid. Mobile phase B consisted of 47.5% acetonitrile, 47.5% isopropanol, and 5% water with 0.1% formic acid. The injection volume was 3 μL and the column temperature was maintained at 40°C.</p>"],"publication":["Analysis of the Mechanism of Pathogenicity Decline in Alternaria alternata f. sp. mali Mediated by the Mycovirus AaCV1."],"submitter_affiliation":["Hebei Agricultural University"],"submitter_name":["jingchu zhang"],"organism_part":["mycelium"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["<p>Approximately 50 ± 5 mg of each sample was placed into a 2 mL centrifuge tube with a 6 mm grinding bead. A total of 400 μL extraction solvent, methanol:water = 4:1 (v/v), containing internal standards including L-2-chlorophenylalanine, was added. Samples were ground for 6 min at -10°C and 50 Hz, followed by ultrasonic extraction for 30 min at 5°C and 40 kHz. The samples were incubated at -20°C for 30 min and centrifuged at 13,000 g for 15 min at 4°C. The supernatant was transferred to autosampler vials for LC-MS analysis. Quality control samples were prepared by pooling 20 μL of supernatant from each sample.</p>"],"organism":["Alternaria alternata"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14366"],"data_transformation_protocol":["<p>Raw LC-MS data were processed using Progenesis QI v3.0 (Waters Corporation, Milford, USA). The workflow included baseline filtering, peak detection, peak extraction, retention time correction, peak alignment, integration, and generation of a data matrix containing retention time, m/z, peak area, and metabolite annotation information.</p>"],"study_factor":["Genotype"],"submitter_email":["Katherinemeowz@126.com"],"sample_collection_protocol":["<p>Thirty fungal samples were collected for untargeted LC-MS metabolomics analysis. Samples were prepared for metabolite extraction and LC-MS/MS detection. Quality control samples were generated by pooling equal aliquots from each sample extract.</p>"],"omics_type":["Metabolomics"],"study_design":["host-pathogen interaction","Thermo Scientific Vanquish UHPLC System","mycelium","Metabolomics","fungal virus","untargeted analysis","Apple leaf blotch disease","Thermo Scientific Exactive","Hebei Agricultural University","experimental blank","Alternaria alternata","data-dependent acquisition"],"curator_keywords":["host-pathogen interaction","Thermo Scientific Vanquish UHPLC System","mycelium","Metabolomics","fungal virus","untargeted analysis","Apple leaf blotch disease","Thermo Scientific Exactive","Hebei Agricultural University","experimental blank","Alternaria alternata","data-dependent acquisition"],"mass_spectrometry_protocol":["<p>Mass spectrometry was performed using a Thermo Scientific Exploris 240 mass spectrometer equipped with an electrospray ionization source. Data were acquired in both positive and negative ion modes. The scan range was m/z 70–1050. The sheath gas flow rate was 60 arb, auxiliary gas flow rate was 20 arb, heater temperature was 350°C, capillary temperature was 320°C, spray voltage was 3400 V in positive mode and -3000 V in negative mode, S-lens RF level was 70, normalized collision energy was 20, 40, and 60%, Full MS resolution was 60,000, and MS2 resolution was 15,000.</p>"],"metabolite_name":["metab_23701","metab_23700","metab_23703","metab_23702","metab_23709","metab_23708","metab_23705","metab_23704","metab_23707","metab_23706","metab_23712","metab_23711","metab_23714","metab_23713","metab_23710","metab_23719","metab_23716","metab_23715","metab_23718","metab_23717","metab_33103","metab_33102","metab_33101","metab_33108","metab_33107","metab_33106","metab_33105","metab_23781","metab_22450","metab_23780","metab_23783","metab_22452","metab_21120","metab_33109","metab_23782","metab_22451","metab_21121","metab_23789","metab_21126","metab_22457","metab_21127","metab_21128","metab_22459","metab_21129","metab_23785","metab_22454","metab_21122","metab_23784","metab_22453","metab_21123","metab_23787","metab_22456","metab_21124","metab_23786","metab_22455","metab_21125","metab_33100","metab_33115","metab_33114","metab_33113","metab_33112","metab_33119","metab_33118","metab_33117","metab_33116","metab_23792","metab_22461","metab_23791","metab_22460","metab_21130","metab_22463","metab_21131","metab_23793","metab_22462","metab_21132","metab_23790","metab_22469","metab_21137","metab_23799","metab_22468","metab_21138","metab_21139","metab_23796","metab_22465","metab_21133","metab_23795","metab_22464","metab_21134","metab_23798","metab_22467","metab_21135","metab_23797","metab_22466","metab_21136","metab_33111","metab_33110","metab_23761","metab_22430","metab_23760","metab_23767","metab_22436","metab_21104","metab_23766","metab_22435","metab_21105","metab_23769","metab_22438","metab_21106","metab_23768","metab_22437","metab_21107","metab_23763","metab_22432","metab_21100","metab_22431","metab_21101","metab_23765","metab_22434","metab_21102","metab_23764","metab_22433","metab_21103","metab_21108","metab_22439","metab_21109","metab_23770","metab_22441","metab_23771","metab_22440","metab_21110","metab_23778","metab_22447","metab_21115","metab_23777","metab_22446","metab_21116","metab_22449","metab_21117","metab_23779","metab_22448","metab_21118","metab_23774","metab_22443","metab_21111","metab_23773","metab_22442","metab_21112","metab_23776","metab_21113","metab_23775","metab_22444","metab_21114","metab_21119","metab_23745","metab_22414","metab_23744","metab_22413","metab_23747","metab_22416","metab_23746","metab_22415","metab_23741","metab_22410","metab_23740","metab_23743","metab_22412","metab_23742","metab_22411","metab_23749","metab_22418","metab_23748","metab_22417","metab_22419","metab_23750","metab_23756","metab_22425","metab_23755","metab_22424","metab_23758","metab_22427","metab_23757","metab_22426","metab_23752","metab_22421","metab_23751","metab_22420","metab_23754","metab_22423","metab_23753","metab_22422","metab_22429","metab_23759","metab_22428","metab_23723","metab_23722","metab_23725","metab_23724","metab_23721","metab_23720","metab_23727","metab_23726","metab_23729","metab_23728","metab_23734","metab_22403","metab_23733","metab_22402","metab_23736","metab_22405","metab_23735","metab_22404","metab_23730","metab_23732","metab_22401","metab_23731","metab_22400","metab_23738","metab_22407","metab_23737","metab_22406","metab_22409","metab_23739","metab_22408","metab_33069","metab_33068","metab_21080","metab_21085","metab_21086","metab_21087","metab_21088","metab_21081","metab_21082","metab_21083","metab_21084","metab_21089","metab_33063","metab_33062","metab_33061","metab_33060","metab_33067","metab_33066","metab_33065","metab_33064","metab_21090","metab_33079","metab_21091","metab_21096","metab_21097","metab_21098","metab_21099","metab_21092","metab_21093","metab_21094","metab_21095","metab_33070","metab_33074","metab_33073","metab_33071","metab_33078","metab_33077","metab_33076","metab_33075","metab_33049","metab_33048","metab_33047","metab_33046","metab_22395","metab_21063","metab_22394","metab_21064","metab_22397","metab_21065","metab_22396","metab_21066","metab_22391","metab_22390","metab_21060","metab_22393","metab_21061","metab_22392","metab_21062","metab_22399","metab_21067","metab_22398","metab_21068","metab_21069","metab_33041","metab_33040","metab_33045","metab_33044","metab_33043","metab_33042","metab_33059","metab_33058","metab_33057","metab_21074","metab_21075","metab_21076","metab_21077","metab_21070","metab_21071","metab_21072","metab_21073","metab_21078","metab_21079","metab_33052","metab_33051","metab_33050","metab_33056","metab_33055","metab_33054","metab_33053","metab_33027","metab_33026","metab_33025","metab_33024","metab_33029","metab_33028","metab_22373","metab_21041","metab_22372","metab_21042","metab_22375","metab_21043","metab_22374","metab_21044","metab_22371","metab_22370","metab_21040","metab_21049","metab_22377","metab_21045","metab_22376","metab_21046","metab_22379","metab_21047","metab_22378","metab_21048","metab_33023","metab_33022","metab_33021","metab_33020","metab_33038","metab_33037","metab_33036","metab_33035","metab_33039","metab_22384","metab_22383","metab_21053","metab_22386","metab_21054","metab_22385","metab_21055","metab_2238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of the Mechanism of Pathogenicity Decline in Alternaria alternata f. sp. mali Mediated by the Mycovirus AaCV1","description":"Apple leaf blotch disease caused by Alternaria alternata is a major fungal disease affecting apple production. To investigate the molecular mechanisms underlying virus-induced hypovirulence, metabolomic profiling was performed on A. alternata strains under different genetic and treatment conditions, including viral protein overexpression strains (P2 and P3) and host gene mutants","dates":{"publication":"2026-04-24","submission":"2026-04-24"},"accession":"MTBLS14366","cross_references":{}}