MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/m_MTBLS14366_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/m_MTBLS14366_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/s_MTBLS14366.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/a_MTBLS14366_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/a_MTBLS14366_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14366<p>Metabolite annotation was performed using Progenesis QI v3.0 by matching MS and MS/MS spectra against public and in-house databases. The main databases included HMDB, METLIN, and the Majorbio in-house database. The MS mass error threshold was set to less than 10 ppm, and metabolite identification was further supported by MS/MS fragmentation matching scores.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>LC separation was performed using a Thermo Scientific Vanquish Horizon UHPLC system. Chromatographic separation was achieved on an ACQUITY UPLC HSS T3 column, 1.8 μm, 2.1 × 100 mm, Waters. Mobile phase A consisted of 95% water and 5% acetonitrile with 0.1% formic acid. Mobile phase B consisted of 47.5% acetonitrile, 47.5% isopropanol, and 5% water with 0.1% formic acid. The injection volume was 3 μL and the column temperature was maintained at 40°C.</p>Analysis of the Mechanism of Pathogenicity Decline in Alternaria alternata f. sp. mali Mediated by the Mycovirus AaCV1.Hebei Agricultural Universityjingchu zhangmyceliummass spectrometry assay<p>Approximately 50 ± 5 mg of each sample was placed into a 2 mL centrifuge tube with a 6 mm grinding bead. A total of 400 μL extraction solvent, methanol:water = 4:1 (v/v), containing internal standards including L-2-chlorophenylalanine, was added. Samples were ground for 6 min at -10°C and 50 Hz, followed by ultrasonic extraction for 30 min at 5°C and 40 kHz. The samples were incubated at -20°C for 30 min and centrifuged at 13,000 g for 15 min at 4°C. The supernatant was transferred to autosampler vials for LC-MS analysis. Quality control samples were prepared by pooling 20 μL of supernatant from each sample.</p>Alternaria alternata<p>Raw LC-MS data were processed using Progenesis QI v3.0 (Waters Corporation, Milford, USA). The workflow included baseline filtering, peak detection, peak extraction, retention time correction, peak alignment, integration, and generation of a data matrix containing retention time, m/z, peak area, and metabolite annotation information.</p>Genotypehttps://www.ebi.ac.uk/metabolights/MTBLS14366Katherinemeowz@126.com<p>Thirty fungal samples were collected for untargeted LC-MS metabolomics analysis. Samples were prepared for metabolite extraction and LC-MS/MS detection. Quality control samples were generated by pooling equal aliquots from each sample extract.</p>Metabolomicshost-pathogen interactionThermo Scientific Vanquish UHPLC SystemmyceliumMetabolomicsfungal virusuntargeted analysisApple leaf blotch diseaseThermo Scientific ExactiveHebei Agricultural Universityexperimental blankAlternaria alternatadata-dependent acquisitionhost-pathogen interactionThermo Scientific Vanquish UHPLC SystemmyceliumMetabolomicsfungal virusuntargeted analysisApple leaf blotch diseaseThermo Scientific ExactiveHebei Agricultural Universityexperimental blankAlternaria alternatadata-dependent acquisition<p>Mass spectrometry was performed using a Thermo Scientific Exploris 240 mass spectrometer equipped with an electrospray ionization source. Data were acquired in both positive and negative ion modes. The scan range was m/z 70–1050. The sheath gas flow rate was 60 arb, auxiliary gas flow rate was 20 arb, heater temperature was 350°C, capillary temperature was 320°C, spray voltage was 3400 V in positive mode and -3000 V in negative mode, S-lens RF level was 70, normalized collision energy was 20, 40, and 60%, Full MS resolution was 60,000, and MS2 resolution was 15,000.</p>metab_23701metab_23700metab_23703metab_23702metab_23709metab_23708metab_23705metab_23704metab_23707metab_23706metab_23712metab_23711metab_23714metab_23713metab_23710metab_23719metab_23716metab_23715metab_23718metab_23717metab_33103metab_33102metab_33101metab_33108metab_33107metab_33106metab_33105metab_23781metab_22450metab_23780metab_23783metab_22452metab_21120metab_33109metab_23782metab_22451metab_21121metab_23789metab_21126metab_22457metab_21127metab_21128metab_22459metab_21129metab_23785metab_22454metab_21122metab_23784metab_22453metab_21123metab_23787metab_22456metab_21124metab_23786metab_22455metab_21125metab_33100metab_33115metab_33114metab_33113metab_33112metab_33119metab_33118metab_33117metab_33116metab_23792metab_22461metab_23791metab_22460metab_21130metab_22463metab_21131metab_23793metab_22462metab_21132metab_23790metab_22469metab_21137metab_23799metab_22468metab_21138metab_21139metab_23796metab_22465metab_21133metab_23795metab_22464metab_21134metab_23798metab_22467metab_21135metab_23797metab_22466metab_21136metab_33111metab_33110metab_23761metab_22430metab_23760metab_23767metab_22436metab_21104metab_23766metab_22435metab_21105metab_23769metab_22438metab_21106metab_23768metab_22437metab_21107metab_23763metab_22432metab_21100metab_22431metab_21101metab_23765metab_22434metab_21102metab_23764metab_22433metab_21103metab_21108metab_22439metab_21109metab_23770metab_22441metab_23771metab_22440metab_21110metab_23778metab_22447metab_21115metab_23777metab_22446metab_21116metab_22449metab_21117metab_23779metab_22448metab_21118metab_23774metab_22443metab_21111metab_23773metab_22442metab_21112metab_23776metab_21113metab_23775metab_22444metab_21114metab_21119metab_23745metab_22414metab_23744metab_22413metab_23747metab_22416metab_23746metab_22415metab_23741metab_22410metab_23740metab_23743metab_22412metab_23742metab_22411metab_23749metab_22418metab_23748metab_22417metab_22419metab_23750metab_23756metab_22425metab_23755metab_22424metab_23758metab_22427metab_23757metab_22426metab_23752metab_22421metab_23751metab_22420metab_23754metab_22423metab_23753metab_22422metab_22429metab_23759metab_22428metab_23723metab_23722metab_23725metab_23724metab_23721metab_23720metab_23727metab_23726metab_23729metab_23728metab_23734metab_22403metab_23733metab_22402metab_23736metab_22405metab_23735metab_22404metab_23730metab_23732metab_22401metab_23731metab_22400metab_23738metab_22407metab_23737metab_22406metab_22409metab_23739metab_22408metab_33069metab_33068metab_21080metab_21085metab_21086metab_21087metab_21088metab_21081metab_21082metab_21083metab_21084metab_21089metab_33063metab_33062metab_33061metab_33060metab_33067metab_33066metab_33065metab_33064metab_21090metab_33079metab_21091metab_21096metab_21097metab_21098metab_21099metab_21092metab_21093metab_21094metab_21095metab_33070metab_33074metab_33073metab_33071metab_33078metab_33077metab_33076metab_33075metab_33049metab_33048metab_33047metab_33046metab_22395metab_21063metab_22394metab_21064metab_22397metab_21065metab_22396metab_21066metab_22391metab_22390metab_21060metab_22393metab_21061metab_22392metab_21062metab_22399metab_21067metab_22398metab_21068metab_21069metab_33041metab_33040metab_33045metab_33044metab_33043metab_33042metab_33059metab_33058metab_33057metab_21074metab_21075metab_21076metab_21077metab_21070metab_21071metab_21072metab_21073metab_21078metab_21079metab_33052metab_33051metab_33050metab_33056metab_33055metab_33054metab_33053metab_33027metab_33026metab_33025metab_33024metab_33029metab_33028metab_22373metab_21041metab_22372metab_21042metab_22375metab_21043metab_22374metab_21044metab_22371metab_22370metab_21040metab_21049metab_22377metab_21045metab_22376metab_21046metab_22379metab_21047metab_22378metab_21048metab_33023metab_33022metab_33021metab_33020metab_33038metab_33037metab_33036metab_33035metab_33039metab_22384metab_22383metab_21053metab_22386metab_21054metab_22385metab_21055metab_22380metab_22382metab_21050metab_22381metab_21051metab_21056metab_22387metab_21057metab_21058metab_22389metab_21059metab_33030metab_33034metab_33033metab_33032metab_33031metab_33005metab_33004metab_33003metab_33002metab_33009metab_33008metab_33007metab_33006metab_23682metab_22351metab_23681metab_22350metab_21020metab_23684metab_22353metab_21021metab_23683metab_22352metab_21022metab_23680metab_22359metab_21027metab_23689metab_22358metab_21028metab_21029metab_23686metab_22355metab_21023metab_23685metab_22354metab_21024metab_23688metab_21025metab_23687metab_22356metab_21026metab_33000metab_33016metab_33015metab_33014metab_33013metab_33019metab_33018metab_33017metab_23693metab_22362metab_21030metab_23692metab_22361metab_21031metab_23695metab_22364metab_21032metab_23694metab_22363metab_21033metab_23691metab_22360metab_23690metab_21038metab_22369metab_21039metab_23697metab_22366metab_21034metab_23696metab_22365metab_21035metab_23699metab_22368metab_21036metab_23698metab_22367metab_21037metab_33012metab_33011metab_33010metab_33081metab_33080metab_33085metab_33084metab_33083metab_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of the Mechanism of Pathogenicity Decline in Alternaria alternata f. sp. mali Mediated by the Mycovirus AaCV1Apple leaf blotch disease caused by Alternaria alternata is a major fungal disease affecting apple production. To investigate the molecular mechanisms underlying virus-induced hypovirulence, metabolomic profiling was performed on A. alternata strains under different genetic and treatment conditions, including viral protein overexpression strains (P2 and P3) and host gene mutants2026-04-242026-04-24MTBLS14366