MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/m_MTBLS14397_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/m_MTBLS14397_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/a_MTBLS14397_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/s_MTBLS14397.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397/a_MTBLS14397_LC-MS_negative_reverse-phase.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14397<p>The identification of metabolites was based on mzCloud, LIPID MAPS, HMDB, MoNA, and self-built databases (Personalbio Co., Ltd., Shanghai, China). The VIP value of metabolites was calculated through multiple cross-validation, and the differential multiples (&gt; 1), P value (&lt; 0.05), and VIP (&gt; 1) of the OPLS-DA model were used to screen differentially expressed metabolites (DEMs).</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>The separation of the chemical compounds was performed on an ACQUITY UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) at a flow rate of 0.4 mL/min. The column was set at 40°C, and the injection volume was 2 μL. The eluents for the positive and negative polarity mode were 0.1% formic acid in water (v/v, A2) and 0.1% formic acid in acetonitrile (v/v, B2). Separation was conducted under the following gradient: 0-1 min, 5% B2; 1-7 min, 5% B2; 7-8 min, 95% B2; 8-8.1 min, 5% B2; 8.1 min, 5% B2; and 12 min, 5% B2.</p>VdGGCT, a GGCT_like super family protein, is required for fungal growth, conidiation and pathogenicity of Verticillium dahliae.Huimin LiPlant Protection Institute, Hebei Academy of Agriculture and Forestry Sciencesmyceliummass spectrometry assay<p>50 mg of samples from Vd991 and the <em>VdGGCT </em>knockout mutant were mixed with 1 mL of pre-cooled 50% methanol in a 2 mL centrifuge tube, add 2 glass beads, and then vortexed for 30 s. Three cycles of treatment were performed: freezing in liquid nitrogen for 5 min, followed by thawing at room temperature and grinding at 55 Hz for 60 s. The mixture was then centrifuged at 12,000 rpm and 4°C for 15 min. Subsequently, 800 μL of the supernatant to dryness under vacuum, and reconstitute with 150 μL of 50% methanol containing 5 ppm 2-chlorophenylalanine by vortexing for 30 s. The mixture was centrifuged at 12000 rpm for 10 min at 4°C. Finally, the supernatant flowed through a 0.22 μm membrane filter and was transferred into the detection bottle for LC-MS detection. The quality control (QC) sample was prepared by mixing equal volumes of the extracts from all samples.</p>Verticillium dahliaehttps://www.ebi.ac.uk/metabolights/MTBLS14397<p>The raw data collected by mass spectrometry was processed by Compound Discoverer 3.0 software (Thermo Fisher Scientific Inc.) for peak extraction, alignment, correction, normalization, and other data preprocessing. Output of a 3D data matrix consisting of sample names, peak information (including retention times and molecular weights), and peak areas was generated.</p>Genotype804149936@qq.com<p>For conidial production and collection, Vd991 and <em>VdGGCT</em> knockout mutant strains grown on PDA for 5 d was used to obtain three mycelial plugs by punching at evenly spaced positions near the colony edge with a 5 mm sterile puncher. The plugs were placed into a 250 mL conical flask containing 100 mL of potato dextrose broth (PDB) medium, and incubated at 25°C with shaking at 180 rpm for 7 d. Conidia of Vd991 and VdGGCT knockout mutant strains were collected by filtration and centrifugation, then resuspended in sterile water to a final concentration. The above spore suspension was inoculated into liquid potato dextrose broth (PDB) medium at a 1% (v/v) inoculum ratio, then incubated in a constant-temperature shaking incubator at 25 °C in the dark, with shaking at 180 rpm for 20 h. The young mycelia were then harvested.</p>Metabolomicsmyceliumfungal metaboliteMetabolomicsLC-MS dataVerticillium dahliaequality controluntargeted analysisThermo Scientific Orbitrap Exploris 120Waters ACQUITY UPLC systemWaters ACQUITY UPC2 Systemexperimental sampleuntargeted metabolite profilingmyceliumfungal metaboliteMetabolomicsLC-MS dataVerticillium dahliaequality controluntargeted analysisThermo Scientific Orbitrap Exploris 120Waters ACQUITY UPLC systemWaters ACQUITY UPC2 Systemexperimental sampleuntargeted metabolite profiling<p>Thermo Orbitrap Exploris 120 mass spectrometer was used to collect DDA mass spectrometric data in positive and negative ion modes under the control of Xcalibur software (version: 4.7, Thermo).The parameters were as follows: sheath gas pressure, 40 arb; aux gas flow, 15 arb; spray voltage, 3.5 kV and -3.0 kV; capillary temperature, 325℃; MS1 range, m/z 100-1000; MS1 resolving power, 60,000 FWHM; number of data dependent scans per cycle, 4; MS/MS resolving power, 15,000 FWHM; normalized collision energy, 30%; and dynamic exclusion time, 8 s.</p><p>All formal samples and QC sample were loaded into the equipment according to the above-mentioned chromatography and mass spectrometry methods. Before the formal injection, QC sample were injected 2-4 times to balance the system. During the injection process, one QC sample was injected for every 5-10 samples for subsequent data evaluation and quality control.</p>2-(Acetylamino)hexanoic acid1,2-Dimethoxybenzene3,3-Dimethylglutaric acid5-Methyl-2-oxo-1,3-oxazolidine-4-carboxylic acid2-Aminonicotinic acidFormylanthranilic acidN-Acetyldopamine2,3-Dihydroxybutanoic acidValylglycinePhloroacetophenoneTrifluoroacetic acidGuaicyl acetateD-Alanyl-D-alanine3-Butene-1,2-diol(Z)-1-(Methylthio)-5-phenyl-1-penten-3-yne4-Aminophenol3-Hydroxypyridine4-Hydroxyhippuric acidAlanylglycine1-MethylxanthineHydroxypyruvic acidthioacroleinN-EthylglycineN-Acetylaspartic acidAcexamic acidL-2,4-Diaminobutyric acidAngelic acidAdipoyl-L-carnitineSarancis,cis-Muconic acid2-HYDROXYBUTYRIC ACIDGlycolic aciddihydroxy acetone(5E)-4-[(E)-3-(4-Hydroxy-3-methoxyphenyl)prop-2-enoyl]oxy-2-methyl-5-[2-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyethylidene]cyclopentene-1-carboxylic acidO-Phenolsulfonic acid4-CARBOXYCINNAMIC ACIDAminooxyacetic acid HemihydrochlorideN-Acetylglutamic acidPolyvidone(E)-3-(indol-3-yl)acrylic acid4-Hydroxycrotonic acid(4-Bromo-2-formylphenoxy)acetic acidN-Heptanoylglycine(1R,4R,5S,6R)-4-Amino-2-oxabicyclo[3.1.0]hexane-4,6-dicarboxylic acid4-Acetamidobenzoic acidisocitric acid3-ChlorophenylhydrazoneD-Threo-3-Pentulose4-Pyridoxic acid4-Hydroxynicotinic acid2,4-Dimethyl-1,3-oxazole-5-carboxylic acid2'-Hydroxyacetophenone3,4-Dihydroxybenzenesulfonic acidPolymaleic acid, sodium saltIsopropyl 5-hydroxy-2-methyl-1H-indole-3-carboxylateMethyleutypinol3-Hydroxy-3-[2-(4-methoxyphenyl)-2-oxoethyl]-1,3-dihydro-2H-indol-2-oneMANNOSEGlyceric acidhept-2-enedioic acidPhthalic acid1,1,1-Trichloroheptane2-Aminoisobutyric acidDimethylmalonic acid2-Furanbutanoic acid, a,g-dioxo-alpha-Hydroxyisobutyric acidL-Malic acid4-HydroxyquinolineOxalic acid4,5-Dihydroxyphthalic acid4-Amino-2-methylenebutanoic acid2-(5-Hydroxy-1H-indol-2-yl)acetic acid2-Chloro-L-phenylalanine-IS(1r,3r)-1-Aminocyclopentane-1,3-dicarboxylic acidMalonic acidoxaloacetic acid 4-methyl ester2-Methylpropyl formate2-MethyltetrahydrofuranTetrahydrofuranylmethyl3-Methylpyrrole-2,4-dicarboxylic acid3-Hydroxymethylglutaric acid3,4-DimethoxyphenolDimethicone1-(4-hydroxyphenyl)ethanol4-Isopropylbenzoic acid4-Oxo-2-azetidinecarboxylic acidBoc-L-Orn-OHL-allo-ThreonineDiethyl phosphate#NAME?beta-tyveloseUric AcidN-LactoylvalinePurineMethyl 3-methylresorcylate2-Octenedioic acid5-Amino-1-(2-fluorophenyl)-1H-pyrazole-4-carboxylic acidOxepinMethylimidazoleacetic acid3-hydroxy-3-methyloxindole4R-aminopentanoic acidEMPSH4-(3-Pyridyl)-3-butenoic acidEglumetad3,5-Dihydroxybenzoic acid(2,5-Dioxotetrahydro-3-furanyl)acetic acid2,4,6-Trimethyl-1,3,5-trithianePhosphoric acid1H-Pyrrole-2-carboxaldehyde.beta.-D-GlucopyranoseO-glutaroyl-L-carnitine2-Ketobutyric acid(S)-2-Azetidinecarboxylic acidL-(-)-ArabitolAcesulfameKetone Ester3-isopropylmalic acid4-propionyl butyric acidLATIFOLICININ C ACID4-(Butoxymethyl)phenolChlorocarbonic acidN-(1H-Indol-3-ylacetyl)methionineSuccinic anhydride2-MethylbutyrylglycineSuberic acidL-Threonic acidAcetylenedicarboxylic acid3-Aminocaproic acid(-)-3-dehydroshikimic acid(S)-2-Amino-4-methylsulfanyl-butane-1-thiol2,6-Dihydroxybenzoic acidAceric acid (3-C-carboxy-5-deoxy-l-xylose) (rare sugar in rhamnogalacturonan II)m-CresolL-(+)-Leucic AcidSalicylic acid3,4-MethylenedioxybenzaldehydeHydroxymethyl hydrogen carbonate3-Acetyl-2,5-dimethylfuranEthyl formateN-Acetylisoleucineethyl 2,4-dihydroxyquinoline-3-carboxylate3-Methyl-2-oxovaleric acidalpha-D-XylopyranoseDiacetylPIMELIC ACID9-(5-O-Methylpentofuranosyl)-1,9-dihydro-6H-purin-6-oneL-methionine-R-sulfoxideMETHYLSUCCINIC ACID2,6-DINITRO-4-FLUOROPHENOLm-hydroxy-Hydrocinnamic acidERYTHRULOSE, D-Indole-3-acrylic acidS-AllylcysteineOrotic acidItaconic acid6-Oxopiperidine-2-carboxylic acid(-)-Camphanic acidMaleic acid3-Mercapto-2-methyl-1-butanolZymonic acidButanoneMetacetamolL(-)-FucoseN-Acetyl-DL-glutamic acid2-Hydroxycaproic acid2-Hydroxy-3-methylbutyric acid2-Aminothiazole1-(2-Furanylmethyl)-1H-pyrrole4-Hydroxyquinoline-3,7-dicarboxylic acidMytilitolGlabrin A3-Cyclohexyl-2-hydroxypropanoic acidPyruvaldehydeEthyl 4-O-(4-carboxy-3-hydroxy-3-methylbutanoyl)-beta-D-glucopyranosidebeta-alanine betaineL-Fucono-1,5-lactone1-Rhamnono-1,4-lactoneHydroxyminaline3,6-dioxo-decanoic acid7-Methyluric acid2-Hydroxy-6-methylpyridine-4-carboxylic acid5-(1-Hydroxyethyl)-3-(2-hydroxypropyl)-2(5H)-furanonep-Cresol3',5'-Cyclic AMP1,3-Cyclohexanedicarboxylic acidL-2-Hydroxyglutaric acidImidazolepropionic acidHydroxyethyl glycineEthanethioic acid2-Hydroxynicotinic acid3-Hydroxymandelic acidPantoic acid4-Acetamidosalicylic acid(E)-4-(6-methyl-1,2-dithiin-3-yl)but-1-en-3-yn-1-olN-IsovalerylglycineGlycine formateLactic acidD-()-RiboseHydroxidodioxidosulfidosulfatefalseLC-MS-based metabolomic analysis of Vd991 and VdGGCT knockout mutant strainsThis study aimed to identify differentially expressed metabolites between Vd991 and VdGGCT knockout mutant strains using liquid chromatography-mass spectrometry (LC-MS). A total of 6 samples, including both Vd991 and VdGGCT knockout mutant groups, were analyzed to explore metabolic changes and discover key metabolites associated with the mutation phenotype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