MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/m_MTBLS14398_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/m_MTBLS14398_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/a_MTBLS14398_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/a_MTBLS14398_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398/s_MTBLS14398.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14398<p>Peak intensities were normalized to the total spectral intensity. The normalized data was used to predict the molecular formula based on additive ions, molecular ion peaks and fragment ions. And then peaks were matched with the mzCloud (https://www.mzcloud.org/)£¬mzVault and MassList database to obtain the accurate qualitative and relative quantitative results.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (Thermo Fisher, Germany) coupled with an Orbitrap Q ExactiveTM&nbsp;HF mass spectrometer. Samples were injected onto a Hypesil Goldcolumn (100¡Á2.1 mm, 1.9¦Ìm) using a 17-min linear gradient at a flow rate of 0.2 mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in Water) and eluent B (Methanol). The eluents for the negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (Methanol).The solvent gradient was set as follows: 2% B, 1.5 min; 2-100% B, 3&nbsp;min; 100% B, 10 min ;100-2% B, 10.1&nbsp;min£»2% B, 12&nbsp;min.</p>Microbiota Reprogramming Driven by 7,8-Dihydroxyflavone Potentiates Xanthine Oxidase to Mitigate Hyperuricemia.Gong liSouth China Agricultural Universitycell culture supernatantmass spectrometry assay<p>The samples (1 mL) were freeze-dried and resuspended with prechilled 80% methanol by well vortex. Then the samples were incubated on ice for 5 min and centrifuged at 15,000 g, 4¡ãC for 15 min. Some of supernatant was diluted to final concentration containing 53% methanol by LC-MS grade water. The samples were subsequently transferred to a fresh Eppendorf tube and then were centrifuged at 15000 g, 4¡ãC for 15 min. Finally, the supernatant was injected into the LC-MS/MS system analysis.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14398Gong li. South China Agricultural University. Wushan Campus, Tianhe District, Guangzhou, Guangdong, China. 1034126372@qq.com.<p>The raw data files generated by UHPLC-MS/MS were processed using the Compound Discoverer 3.1 (CD3.1, ThermoFisher) to perform peak alignment, peak picking, and quantitation for each metabolite. The main parameters were set as follows: retention time tolerance, 0.2 minutes; actual mass tolerance, 5ppm; signal intensity tolerance, 30%; signal/noise ratio, 3; and minimum intensity, et al.</p>Treatment1034126372@qq.com<p>The samples used in this study were supernatants collected from bacterial liquid cultures. Two groups of samples were included:</p><p>1. DHF treatment group (B): Bacteria were cultured in medium supplemented with 7,8-dihydroxyflavone (DHF).</p><p>2.Control group (D): Bacteria were cultured in standard medium without any compound supplementation.</p><p>At the end of the culture period, bacterial cultures were centrifuged at 10,000 g for 10 min at 4¡ãC to remove cell pellets. The resulting supernatants were collected and immediately used for metabolite extraction without long-term frozen storage. All samples were processed in parallel to minimize technical variation.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicshyperuricemiaMus musculusuntargeted analysisGastrointestinal Microbiomecell culture supernatant7,8-dihydroxyflavoneThermo Scientific Q Exactive HFXanthine Oxidaseexperimental sampleThermo Scientific Vanquish UHPLC SystemMetabolomicshyperuricemiaMus musculusuntargeted analysisGastrointestinal Microbiomecell culture supernatant7,8-dihydroxyflavoneThermo Scientific Q Exactive HFXanthine Oxidaseexperimental sample<p>Q ExactiveTM&nbsp;HF mass spectrometer was operated in positive/negative polarity mode with spray voltage of 3.5&nbsp;kV, capillary temperature of 320¡ãC, sheath gas flow rate of 35&nbsp;psi&nbsp;and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heater temperature of 350¡ãC.</p>Gamma-Glu-LeuCaprolactamMonolaurinL-PipecolatedihydrotachysterolN-AcetyldopaminePro-Leuo-Toluic Acid8-Aminooctanoic acid4-Guanidinobutyric acid9-KODEL-HistidineAndrographolideNoradrenalineCholest-4-en-3-oneDL-o-TyrosinePyridoxalMonensinVasicinonePalmitoylethanolamideD-SphingosineN-lactoyl-phenylalanine2,3-Dinor-TXB2BiotinN-PropionylglycineIsopropyl myristateGuggulsteroneStyreneDeoxycorticosteroneL-5-Hydroxytryptophan4-HydroxybenzaldehydeIndirubinStearoyl Ethanolamide2,6-Di-tert-butyl-1,4-benzoquinoneL-lysineCitrininProline-hydroxyprolineCarnosinePilocarpineJasmoneN6-Acetyl-L-lysine3-Methoxytyramine2-MethoxyestroneOctanedioic acidRicinineL-Tryptophantrans-4-Hydroxy-L-prolineBoldione2-Oxindole6-MethylquinolineDopamineSerotonin5-HydroxytryptopholN-Stearoyl taurine5-Hydroxylysine4-Guanidinobutanoic acidIsophorone4-Methyl-5-thiazoleethanolLeucylproline1,4-Dihydro-1-Methyl-4-Oxo-3-PyridinecarboxamideOleamideIsotretinoinEicosapentaenoic acid10-Undecenoic acidTryptamineCuminaldehydeAla-IleL-KynurenineNNK7-Ketocholesterol4-Acetamidobutyric Acid2-HydroxyphenylalanineN-FormylkynurenineMethanandamide19-NortestosteroneCiprosteneGlycerol 1-hexadecanoateErgocalciferol3-Ureidopropionic acidN,N-DimethylsphingosineGuanidinosuccinic acidQuinineCoumarinPalmitoyl sphingomyelinSpermineHypoxanthineDutasteride2-PhenylglycinePalmitoyl ethanolamideVitamin B2Hydroxyprogesterone caproateIndoleN-(5-Aminopentyl)acetamideN8-Acetylspermidine10-Nitrolinoleate4-Methylhippuric acidN6,N6,N6-Trimethyl-L-lysine5-Hydroxyindole(S)-beta-Aminoisobutyric Acid13-OxoODEThiamine2-Hydroxyisocaproic AcidPalmitoleic AcidDL-Norvaline2-Hydroxycinnamic acidCortisoneGly-PheD-ProlineL-TyrosineAla-trpAdipamideD-PhenylalanineMethylimidazoleacetic acidL-Phenylalanine5-MethylcytosineTetradecanedioic acid7-MethyladenineOleanolic acidGlycylprolineN-Palmitoyl taurineAnserineSpermidineL-(-)-MethionineN-Oleoyl Glycine1-Oleoyl-Sn-Glycero-3-PhosphocholineStearamideOxymorphoneCaffeineNatamycinNervonic acid3b,7b-Dihydroxy-5-androsten-17-oneSedanolideSebacic acid4-Aminobenzoic acidN-AcetylhistamineValidamycin ANicotinamidePhylloquinone2,4-DimethylbenzaldehydeTetramethylpyrazineUreidoisobutyric AcidFormononetinp-Mentha-1,3,8-trieneAzetidine-2-carboxylic acidMetanephrineDL-TryptophanAmikacinGuanineThr-LeuO-Acetyl-L-carnitinePiperine1-MethylnicotinamideAcetophenonePyridoxamineCreatinine9-Oxo-ODEAcetanilideN-Acetylputrescine2-PhenylethylaminePyridoxineAndrosterone6-AminonicotinamideEpitestosterone7-Methylguanine2,3,5,6-TetramethylpyrazineNorcodeineCholineLevalbuterolBeta-Muricholic acidAcetylcholine5-OxoETECholine bitartrateLeucine-enkephalin2-(3,4-dimethoxyphenyl)ethanamineAstaxanthinLinoleoyl ethanolamideTiglic acidSPHCortisolPipecolic acidUrsodeoxycholic acidL-Alanyl-L-prolinePantetheineVal-SerBicyclo Prostaglandin E2TryptophanolNicotinamide N-oxideN-MethylhydantoinAMK3-Amino-4-methylpentanoic acidAsp-PheUndecanedioic acid2-Amino-1,3-octadecanediolT-2 TriolHydroquinoneHistamineNicotinic AcidNoroxymorphoneDiosgeninP-Aminohippuric AcidEthyl oleatecis-gondoic acidEcgonine methyl esterN-BenzylformamideJervine17alpha-Ethinyl estradiolHexadecanamideL-Alanyl-L-Lysine5-Methyl-2'-deoxycytidineGly-TyrDihydrothymineL-AdrenalineSphinganineCadaverineN-Acetyl-L-leucine7-Ketolithocholic acidCholecalciferolThymineindole-5,6-quinone4-Aminobiphenyl9,10-DihomePhysostigmine5beta-Androstane-3,17-dioneValylprolineDeoxycytidineEstroneXMPfalseMicrobiota Reprogramming Driven by 7,8-Dihydroxyflavone Potentiates Xanthine Oxidase to Mitigate HyperuricemiaHyperuricemia (HUA), primarily driven by xanthine oxidase (XO)-mediated uric acid production, presents substantial challenges to both human health and livestock productivity. While conventional XO inhibitors are effective in mitigating HUA, they are often linked to severe adverse effects. In this study, we developed MiXO, an advanced high-throughput microbiome-based screening framework aimed at identifying natural products that inhibit uric acid (UA) production through microbiota-mediated XO inhibition. From a library of 131 compounds, 7,8-dihydroxyflavone (7,8-DHF) was identified as a primary candidate. In HUA mouse models, treatment with 7,8-DHF normalized serum UA levels and reduced hyperuricemia-associated pathological sequelae and renal dysfunction. Metagenomic and metabolomic analyses demonstrated that 7,8-DHF enhances the abundance of L. reuteri, which promotes the accumulation of the microbial co-metabolite catechin. Molecular docking simulations, along with subsequent in vivo validation, confirmed that catechin occupies the XO catalytic pocket, effectively providing a biochemical blockade of urate biosynthesis to alleviate hyperuricemia. Collectively, our findings establish MiXO as a robust platform for microbiome-targeted drug discovery and position 7,8-DHF as a versatile therapeutic candidate for mitigating HUA.2026-04-292026-04-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