MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/m_MTBLS14399_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/m_MTBLS14399_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/a_MTBLS14399_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/a_MTBLS14399_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399/s_MTBLS14399.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14399<p>The data were analyzed through the free online platform of majorbio choud platform (cloud.majorbio.com). Lipidomic features detected at least 80 % in any set of samples were retained. After filtering, minimum metabolite values were imputed for specific samples in which the metabolite levels fell below the lower limit of quantitation and each Metabolic features were normalized by sum. In order to reduce the errors caused by sample preparation and instrument instability, the response intensity of the sample mass spectrum peaks was normalized by the sum normalization method, and the normalized data matrix was obtained. At the same time, variables with relative standard deviation (RSD) &gt; 30% of QC samples were removed, and log10 logarithmization was performed to obtain the final data matrix for subsequent analysis.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>The LC-MS/MS analysis of sample was conducted on a Thermo Vanquish H/Q-Exactive HF-X-4 equipped with an Accucore C30 column (100 mm × 2.1 mm i.d., 2.6 µm; Thermo, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). The mobile phases consisted of 10 mM ammonium acetate in ACN: H2O (1:1, v/v) (0.1% (v/v) formic acid) (solvent A), and 2 mM ammonium acetate in ACN: IPA: H2O (10:88:2, v/v/v) (0.02% (v/v) formic acid). The standard sample injection parameters were as follows: volume: 2 µL, flow rate: 0.4 ml/min, and column temperature: 40 ℃, 20 minutes of total chromatographic separation. During the period of analysis, all these samples were stored at 4oC.</p>Milk extracellular vesicles attenuate high-fat diet and antibiotic-induced pre-inflammatory bowel disease through Lactobacillus murinus-mediated TLR2 signaling modulation.Shandong First Medical University & Shandong Academy of Medical SciencesJiarun XuColonmass spectrometry assay<p>For lipid extraction, 50mg solid sample were accurately weighed into 2 mL plastic microtubes, and mixed with 280 µL methanol:water (2:5, v/v) solution and 400 µL MTBE. Before extraction, a 6 mm grinding bead was added to each sample. Samples were then homogenated at -10oC by High throughput tissue crusher Wonbio-96c( Shanghai wonbio technology co., LTD) operating at a frequency of 50 Hz for 6 min, then followed by sonicated at 40 kHz for 30 min at 5oC. Subsequently, the samples were placed at -20oC for 30min, followed with centrifugation at 13000g at 4oC for 15min. 350 µL lipid extracts which contained in the upper phase were transferred to new tubes and evaporated to dryness under a gentle stream of nitrogen. For UHPLC-MS/MS anlaysis, the samples were reconstituted in 100 µL loading solution of isopropanol : acetonitrile (1:1, v/v) by brief sonication in a 5oC water bath. Extracted lipids were spun for 15 min at 13000g at 4oC on a bench-top centrifuge and cleared supernatant were transferred to sample vials, then 2 µL portions of each sample were injected into the UHPLC-MS/MS system.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14399Jiarun Xu. Shandong First Medical University & Shandong Academy of Medical Sciences. brucesleeving@163.com.Lingjun Tong. ljtong@sdfmu.edu.cn.<p>After UPLC-MS/MS analyses, the raw data were imported into the LipidSearch (Thermo, CA) for peak detection, alignment and identification. The lipids were identified by MS/MS fragments. Mass tolerance for precursor and fragment was both set to 10 ppm. The displayed m-score threshold was set as 2.0 and grades A,B ,C,D were all used for ID quality filter.The preprocessing results generated a data matrix that consisted of the lipid class, retention time (RT),mass-to-charge ratio (m/z) values, and peak intensity.</p>MEVsPre-IBDBrucesleeving@163.com<p>Mice were sacrificed and colon tissues were collected for lipidomics analysis.</p>MetabolomicsVanquish HMus musculusuntargeted analysisQ-Exactive HF-X-4LipidomicsColonVanquish HMus musculusuntargeted analysisQ-Exactive HF-X-4LipidomicsColon<p>&nbsp;The mass spectrometric data was collected using a Thermo Vanquish H/Q-Exactive HF-X-4 equipped with an electrospray ionization (ESI) source operating in positive and negative ion mode. The optimal conditions were set as followed: Sheath gas flow rate at 60 psi; Aus gas flow rate at 20 psi; Aus gas heater temperature at 370 oC; ion-spray voltage floating (ISVF) at -3000V in negative mode and at +3000V in positive mode, respectively; Normalized collision energy, 20-40-60V rolling for MS/MS. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 200-2000 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of colon tissues<p>Pre-IBD is a transitional state between irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD), in which chronic high-fat diet intake and antibiotic exposure significantly increase intestinal inflammatory susceptibility. However, the underlying mechanisms and effective therapeutic strategies remain inadequately understood. Milk-derived extracellular vesicles (mEVs) have emerged as promising nanotherapeutic agents for alleviating gut disorders. In this study, we found that pre-IBD mice exhibited profound gut microbiota dysbiosis, and treatment with mEVs significantly alleviated intestinal inflammation by modulating MAPK-mediated M1/M2 macrophage polarization and restoring microbiota balance. Mechanistically, mEVs treatment led to a reduction in lysophosphatidylcholine (LPC) levels and an enrichment of Lactobacillus murinus (L. murinus), which exerted opposing effects on intestinal inflammation in both in vivo and in vitro models. Specifically, LPC exacerbated intestinal inflammation and structural alterations, whereas L. murinus treatment mitigated these pathological changes. Notably, both LPC and L. murinus interact with TLR2 but activate distinct downstream signaling pathways. LPC activates the TLR2/MAPK/NF-κB axis, promoting M1 polarization, whereas L. murinus binding to TLR2 induces M2 polarization. These findings demonstrate that mEVs reprogram the TLR2-mediated immune response by modulating the levels of L. murinus and LPC, thereby mitigating gut inflammation and contributing to the attenuation of pre-IBD pathology.</p>2026-04-292026-04-29MTBLS14399HMDB0013404;HMDB0007969;HMDB0013221;LMGP01010566HMDB0061699;LMGP01050121;LMGP01050140;HMDB0010396;LMGP01050048;HMDB0010395HMDB0011207;HMDB0008002;LMGP01010684;HMDB0013411;LMGP01010682HMDB0010392;LMGP01050132HMDB0007938;LMGP01010541;HMDB0007964;HMDB0007939HMDB0008748;LMGP01011119HMDB0012097;LMSP03010028HMDB0010406;LMGP01050144HMDB0010401;LMGP01050124HMDB0008616HMDB0011287;HMDB0008147;LMGP01010943;HMDB0011319;HMDB0008148;HMDB0011320;HMDB0011286HMDB0240262;LMGP01050074;LMGP01050113;LMGP01050018;HMDB0010382HMDB0008443;LMGP01011052;HMDB0008444;HMDB0008477;HMDB0008476;LMGP01011047HMDB0007935;LMGP01010532;HMDB0007961;PW_C003985HMDB0010381;HMDB0010375;LMGP01050016HMDB0007949;HMDB0007950HMDB0008016;HMDB0011221;LMGP01011305;HMDB0013415;LMGP01010695;HMDB0008015;HMDB0011220HMDB0010400;LMGP01050135HMDB0011129;LMGP02050038;LMGP02050001;HMDB0011130HMDB0007998;HMDB0011203HMDB0007958HMDB0008313;HMDB0008312;HMDB0013444HMDB0011222;HMDB0008017HMDB0008419;HMDB0008387HMDB0011229;LMGP01011306;HMDB0008023;LMGP01010696HMDB0011212;HMDB0008008;HMDB0008007;HMDB0011213HMDB0011256;HMDB0013433;HMDB0008117;LMGP01010839;LMGP01010845;HMDB0008084;LMGP01010909HMDB0010457;HMDB0005453;LMGL03012612;HMDB0049282;LMGL03012613;LMGL03010250;HMDB0049261;HMDB0049262HMDB0007942;HMDB0007941HMDB0009816;HMDB0009815;LMGP06010010HMDB0010387;LMGP01050038;HMDB0010388;LMGP01050128HMDB0007248HMDB0013409;LMGP01010650;HMDB0007991HMDB0008233HMDB0008649HMDB0007990;LMGP01010645;HMDB0007989;LMGP01010647HMDB0008640;HMDB0008641HMDB0008715;HMDB0008682HMDB0008452;HMDB0008485HMDB0240629;LMGP01010720HMDB0007948;HMDB0007947HMDB0012087;LMSP03010020HMDB0008411;HMDB0008379;HMDB0008378;HMDB0008410HMDB0008222;HMDB0008189HMDB0044116;HMDB0044088;HMDB0044089;HMDB0303020;HMDB0005382HMDB0007956;HMDB0007957HMDB0008286;HMDB0008287;LMGP01011027HMDB0005433;HMDB0048590HMDB0010458;HMDB0049267;HMDB0049288;HMDB0005455HMDB0044918;HMDB0044944;HMDB0044917;HMDB0005403HMDB0048758;HMDB0048751HMDB0049285;HMDB0010463;HMDB0049291;HMDB0049270;HMDB0049264;HMDB0049883;HMDB0010462;HMDB0049889HMDB0007951HMDB0011711HMDB0007121HMDB0008706;HMDB0008707;HMDB0008673;HMDB0008674HMDB0008551;HMDB0013450HMDB0062701HMDB0044929;HMDB0044956HMDB0008310;HMDB0008311HMDB0052501HMDB0010411HMDB0008321HMDB0049277;HMDB0049890;HMDB0049298;HMDB0049271;HMDB0005456;HMDB0049292;HMDB0049916;HMDB0049896HMDB0008336HMDB0005404;HMDB0044919HMDB0010418HMDB0104223HMDB0044912;HMDB0005402HMDB0048763HMDB0010431;HMDB0044729HMDB0044060;HMDB0005377HMDB0007228;HMDB0007200;HMDB0007199;HMDB0007229HMDB0010466;HMDB0049286;HMDB0049265HMDB0050494;HMDB0050475HMDB0049391;HMDB0050004HMDB0005440;HMDB0048618HMDB0008583HMDB0049287;HMDB0049885;HMDB0049905;HMDB0049266HMDB0048634;HMDB0048613;HMDB0005438;HMDB0048612HMDB0008005;LMGP01010678;HMDB0008029;LMGP01010687;HMDB0008004;HMDB0011210;LMGP01010688;HMDB0011209HMDB0007112;HMDB0007113HMDB0043032HMDB0044275HMDB0049399;HMDB0050012HMDB0043836;HMDB0010414HMDB0007179HMDB0043206HMDB0011708;HMDB0043235HMDB0050019;HMDB0049406HMDB0043019;PW_C023733HMDB0044555;HMDB0044387HMDB0103926HMDB0010412HMDB0048567HMDB0010417;HMDB0043899HMDB0048641;HMDB0048619;HMDB0048626;HMDB0048648HMDB0007190;HMDB0007219HMDB0008781HMDB0044270;HMDB0044263HMDB0044079HMDB0049714;HMDB0049079;HMDB0049707;HMDB0049086HMDB0008280;HMDB0008279;LMGP01011023;HMDB0013442HMDB0048574HMDB0049273;HMDB0010467;HMDB0049892;HMDB0049294HMDB0008382;HMDB0008414HMDB0007176HMDB0044272;HMDB0005391HMDB0007403;HMDB0007402HMDB0043025HMDB0043846;HMDB0043852HMDB0052631;HMDB0005475HMDB0050595HMDB0049296;HMDB0010459;HMDB0049894;HMDB0049887;HMDB0049268;HMDB0049289;HMDB0049275;HMDB0010460HMDB0044129;HMDB0044134;HMDB0044101;HMDB0044106HMDB0007955HMDB0043417;HMDB0043410HMDB0049396;HMDB0050003;HMDB0050009;HMDB0049390HMDB0049448;HMDB0050059HMDB0044914;HMDB0044941HMDB0007177;HMDB0007178HMDB0042967HMDB0007391;HMDB0007392HMDB0050098;HMDB0049489;HMDB0049610;HMDB0050093;HMDB0049484;HMDB0049615;HMDB0050213;HMDB0050218HMDB0007131;HMDB0007130HMDB0053463HMDB0050016;HMDB0049403;HMDB0049397;HMDB0005462HMDB0008064;HMDB0008097;LMGP01010882;HMDB0011236HMDB0007237;HMDB0007208HMDB0005411HMDB0044132;HMDB0005385;HMDB0044104;HMDB0044098HMDB0093939HMDB0008255HMDB0008303HMDB0053055;HMDB0010507;HMDB0053062HMDB0007119;HMDB0007120HMDB0005461;HMDB0049393HMDB0043201;HMDB0043200HMDB0005446HMDB0044268HMDB0049913;HMDB0049898;HMDB0049279;HMDB0049295;HMDB0010468;HMDB0049893;HMDB0049300HMDB0044734HMDB0005362HMDB0005394;HMDB0045452HMDB0008236;HMDB0008235;HMDB0006292HMDB0007103HMDB0007161HMDB0044112;HMDB0044084HMDB0007118HMDB0043199HMDB0044747HMDB0008285HMDB0048631;HMDB0048653HMDB0044303;HMDB0044499HMDB0048756HMDB0044128;HMDB0044100HMDB0045748;HMDB0045722HMDB0045742;HMDB0045717;HMDB0045716;HMDB0045743HMDB0050602HMDB0007167HMDB0044093;HMDB0044121HMDB0007548;HMDB0007519;HMDB0007518;HMDB0007547HMDB0045368;HMDB0045206HMDB0049441;HMDB0050052HMDB0047374HMDB0007986;LMGP01010638HMDB0007364HMDB0007172HMDB0044774;HMDB0045476HMDB0048422HMDB0005447;HMDB0048760HMDB0007225;HMDB0007196HMDB00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