Multiple reaction monitoring (MRM) has been used for quantification of screening fragment ions.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - reverse-phase","Liquid Chromatography MS - positive - normal-phase"],"chromatography_protocol":["Polyamines:
Chromatographic separation was performed on an Agilent 1290 Infinity UHPLC system (Agilent Technologies, USA) equipped with an XBridge BEH C18 column (2.5 μm, 2.1 × 100 mm). The mobile phase consisted of 10 mM ammonium formate?0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). The gradient elution program was as follows: 0?1 min, 5% B; 1?1.5 min, 95% B; 1.5?3 min, 95% B, with a post time of 3 min. The flow rate was 0.4 mL/min, injection volume 1 μL, and column temperature 25 °C.
Amino acids:
Chromatographic separation was performed on a SHIMADZU LC-20A system (Shimadzu Corporation, Japan) equipped with an XBridge BEH Amide column (2.5 μm, 2.1 × 100 mm). The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B), with an isocratic elution of 30% A and 70% B for 5 min. The flow rate was 0.4 mL/min, injection volume 1 μL, and column temperature 25 °C.
"],"publication":["AOC1 regulates labor initiation through spermidine-induced autophagy of placental trophoblast cells mediated by EIF5A hypusination."],"submitter_name":["Huaiyan Chen"],"submitter_affiliation":["naval medical university"],"organism_part":["placenta"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Placental tissue samples (approximately 10 mg each) were precisely weighed and placed into 2 mL EP tubes. A steel ball and 100 μL of 80% methanol were added, followed by homogenization at 60 Hz for 3 min. The homogenates were centrifuged at 15,000 ×g for 20 min at 4 °C. The supernatants were collected and diluted with 80% methanol accordingly. For polyamine analysis, the samples were diluted 10-fold. For amino acid analysis, the samples were diluted 10-fold and 100-fold, and then mixed 1:1 with an internal standard solution (200 ng/mL). All samples were vortexed and transferred into vials for subsequent analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14423"],"author":["Huaiyan Chen. chenhuaiyansky@sina.com.","Lu Gao. Naval Medical University. roadgao@163.com."],"data_transformation_protocol":["Standard curves were constructed for quantification. For polyamines, mixed standard stock solutions (100 μg/mL) were serially diluted with 80% methanol to obtain working solutions of various concentrations. For amino acids, the serially diluted standards were mixed 1:1 with internal standard solution (200 ng/mL). All standard working solutions and sample solutions were analyzed under the above chromatographic and mass spectrometric conditions. Each sample solution was injected in duplicate. Peak determination and peak area integration were performed with MassHunter Workstation software (Agilent, Version B.08.00) for polyamines and LabSolutions LCMS (Shimadzu, Version 6.50) for amino acids. Standard curves were plotted with the standard concentration (X) versus the peak area (Y) for polyamines, or versus the peak area ratio of analyte to internal standard (Y) for amino acids. Sample concentrations were calculated based on the corresponding standard curves and dilution factors. Quantitative detection of amino analytes by UPLC-MS/MS using an optimized method previously reported.
"],"study_factor":["Genotype"],"submitter_email":["chenhuaiyansky@sina.com"],"sample_collection_protocol":["Our tissues were derived from the placentas of steroid hormone receptor coactivator deficient mice at gestational day 18.5. All tissue samples were immediately snap-frozen in liquid nitrogen after removal from the body and stored at ?80 °C until analysis. For polyamine quantification, a total of 10 mouse placental tissue samples were analyzed (5 Src-1/-2 dKO placentas and 5 wild-type controls). For amino acid quantification, a total of 10 mouse placental tissue samples were analyzed (5 Src-1/-2 dKO placentas and 5 wild-type controls). Each group consisted of 5 independent biological replicates (i.e., 5 individual mouse placentas). No technical replicates were included in the metabolomics analysis; each biological sample was extracted and analyzed once.
"],"omics_type":["Metabolomics"],"study_design":["Finnish Mass Spectrometry Society","ProteoWizard msconvert","Metabolomics","Agilent 6470 Triple Quadrupole","Shimadzu LCMS-8040","Mus musculus","targeted analysis","Shimadzu LC-30A Nexera UHPLC system","Post-term delivery","placenta","Agilent 1290 Infinity UHPLC","experimental sample"],"curator_keywords":["Finnish Mass Spectrometry Society","ProteoWizard msconvert","Metabolomics","Agilent 6470 Triple Quadrupole","Shimadzu LCMS-8040","Mus musculus","targeted analysis","Shimadzu LC-30A Nexera UHPLC system","Post-term delivery","placenta","Agilent 1290 Infinity UHPLC","experimental sample"],"mass_spectrometry_protocol":["Polyamines: mass spectrometric detection was carried out on an Agilent 6470 triple quadrupole MS/MS system (Agilent Technologies, USA) in positive ion mode. The optimized parameters were as follows: gas temperature 350 °C, gas flow 11 L/min, nebulizer 40 psi, sheath gas heater 350 °C, sheath gas flow 11 L/min, and capillary voltage 4000 V.The multiple reaction monitoring (MRM) transitions were m/z 203.1 to 129.1 (fragment 95 V, CE 15 V) for spermine, m/z 146.2 to 129.1 (fragment 80 V, CE 8 V) for spermidine, m/z 89.0 to 30.2 (fragment 80 V, CE 25 V) for putrescine.
Amino acids: mass spectrometric detection was carried out on a SHIMADZU LCMS 8040 triple quadrupole MS/MS system (Shimadzu Corporation, Japan) in positive ion mode. The instrument parameters were as follows: nebulizing gas flow 3 L/min, DL temperature 250 °C, heat block temperature 400 °C, and drying gas flow 15 L/min. MRM transitions were m/z 176.1 to 159.1 (CE -10 V) for citrulline, m/z 175.1 to 116.1 (CE -14 V) for arginine, m/z 133.1 to 116.1 (CE -15 V) for ornithine, and m/z 181.1 to 74.1 (CE -21 V) for the internal standard.
"],"additional_accession":[]},"is_claimable":false,"name":"The targeted metabolomics data of the post-term mouse model","description":"We performed targeted metabolomics analysis on placental tissues from post term mouse models generated by double knockout of steroid hormone receptor coactivators 1 and 2, as well as from wild type mouse placentas. The levels of polyamines (putrescine, spermidine and spermine) and amino acids (citrulline, arginine and ornithine) were measured.
","dates":{"publication":"2026-05-14","submission":"2026-05-04"},"accession":"MTBLS14423","cross_references":{}}