At the same time, the metabolites were identified by searching database, and the main databases were the HMDB (http://www.hmdb.ca/), Metlin ( https://metlin.scripps.edu/) and Majorbio Database .
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["The LC-MS/MS analysis of sample was conducted on a Thermo UHPLC-Q Exactive HF-X system equipped with an ACQUITY HSS T3 column (100 mm × 2.1 mm i.d., 1.8 μm; Waters, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). The mobile phases consisted of 0.1% formic acid in water:acetonitrile (95:5, v/v) (solvent A) and 0.1% formic acid in acetonitrile:isopropanol:water (47.5:47.5, v/v) (solvent B). The flow rate was 0.40 mL/min and the column temperature was 40℃. The injection volume was 3 μL.
"],"publication":["Urinary Ubiquitin-Specific Protease 2 (USP2) as a Diagnostic Biomarker for Chronic Kidney Disease: An Integrated Multi-Omics and Network Analysis."],"submitter_name":["Qinyuan Deng"],"submitter_affiliation":["The First People's Hospital of Yunnan Province"],"organism_part":["urine","Serum"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["100 μL liquid sample was added to a 1.5 mL centrifuge tube with 400 μL solution (acetonitrile: methanol = 1:1(v:v)) containing 0.02 mg/mL internal standard (L-2-chlorophenylalanine) to extract metabolites.The samples were mixed by vortex for 30 s and low-temperature sonicated for 30 min (5°C, 40 KHz). The samples were placed at -20°C for 30 min to precipitate the proteins.Then the samples were centrifuged for 15 min (4°C, 13000 g).The supernatant was removed and blown dry under nitrogen. The sample was then re-solubilized with 100 µL solution (acetonitrile: water = 1:1) and extracted by low-temperature ultrasonication for 5 min (5°C, 40 KHz), followed by centrifugation at 13000 g and 4°C for 10 min.The supernatant was transferred to sample vials for LC-MS/MS analysis.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14426"],"author":["Qinyuan Deng. First People's Hospital of Yunnan Province. deng835552032@gmail.com."],"data_transformation_protocol":["The pretreatment of LC/MS raw data was performed by Progenesis QI (Waters Corporation, Milford, USA) software, and a three-dimensional data matrix in CSV format was exported. The information in this three-dimensional matrix included: sample information, metabolite name and mass spectral response intensity. Internal standard peaks, as well as any known false positive peaks (including noise, column bleed, and derivatized reagent peaks), were removed from the data matrix, deredundant and peak pooled
"],"study_factor":["Treatment"],"submitter_email":["deng835552032@gmail.com"],"sample_collection_protocol":["Urine and fasting serum samples were collected, centrifuged, and stored at −80°C before LC-MS/MS analysis. QC samples were included during the run.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Biomarker","urine","untargeted analysis","Thermo Scientific Q Exactive HF-X","USP2","Homo sapiens","Serum","experimental blank","Chronic Kidney Disease","untargeted metabolite profiling"],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Biomarker","urine","untargeted analysis","Thermo Scientific Q Exactive HF-X","USP2","Homo sapiens","Serum","experimental blank","Chronic Kidney Disease","untargeted metabolite profiling"],"mass_spectrometry_protocol":["The mass spectrometric data were collected using a Thermo UHPLC-Q Exactive HF-X Mass Spectrometer equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode. The optimal conditions were set as followed: source temperature at 425℃; sheath gas flow rate at 50 arb; Aux gas flow rate at 13 arb; ion-spray voltage floating (ISVF) at -3500V in negative mode and 3500V in positive mode, respectively; Normalized collision energy , 20-40-60V rolling for MS/MS. Full MS resolution was 60000, and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 70-1050 m/z.
"],"additional_accession":[]},"is_claimable":false,"name":"Urinary Ubiquitin-Specific Protease 2 (USP2) as a Diagnostic Biomarker for Chronic Kidney Disease: An Integrated Multi-Omics and Network Analysis","description":"Urinary USP2 is elevated in chronic kidney disease and shows strong diagnostic performance. Integrated metabolomics and proteomics analyses identified cortisol and prostaglandin A3 as candidate mediators and linked USP2 to lipid metabolism and ferroptosis-related pathways.","dates":{"publication":"2026-05-05","submission":"2026-05-05"},"accession":"MTBLS14426","cross_references":{}}