MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/m_MTBLS14431_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/m_MTBLS14431_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/a_MTBLS14431_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/s_MTBLS14431.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431/a_MTBLS14431_LC-MS_negative_reverse-phase.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14431<p>Metabolites were identified by comparing mass spectra and retention times with public databases such as HMDB, METLIN, and KEGG. Annotation was based on accurate mass and MS/MS fragmentation patterns.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using an ultra-high performance liquid chromatography system equipped with a C18 column. The mobile phase consisted of water with 0.1% formic acid (A) and acetonitrile (B), using a gradient elution program. The flow rate was set at 0.3 mL/min, column temperature at 40 °C, and injection volume at 2 μL.</p>Provisional title: Effects of Scytosiphon lomentaria polysaccharides on Vibrio harveyi infection in Litopenaeus vannamei.Qingdao University of Science and Technologycc ssWhole Organismmass spectrometry assay<p>Metabolites were extracted by adding cold methanol/acetonitrile/water (2:2:1, v/v/v) to the samples, followed by vortexing and sonication. After incubation at −20 °C, samples were centrifuged, and the supernatant was collected for LC-MS analysis. Quality control (QC) samples were prepared by pooling equal aliquots from all samples.</p>Vibrio harveyihttps://www.ebi.ac.uk/metabolights/MTBLS14431yi gao. Qingdao Agricultural University. gaoyi@qau.edu.cn.su liu.<p>Raw LC-MS data were processed using standard software for peak detection, alignment, and normalization. The resulting data matrix was subjected to multivariate statistical analysis, including PCA and OPLS-DA.</p>Treatment benefitcatmimidogdudu@163.com<p>Bacterial samples (Vibrio harveyi) were cultured under standard laboratory conditions. The treatment group was exposed to polysaccharide, while the control group received no treatment. After incubation, cells were collected by centrifugation, washed with sterile saline, immediately frozen in liquid nitrogen, and stored at −80 °C until analysis.</p>Metabolomicsultra-performance liquid chromatography-mass spectrometryMetabolomicscentroid spectrumWaters ACQUITY UPLC H-Class Systemuntargeted analysisMassLynxQingdao Agricultural UniversityProgenesis QIWhole Organismexperimental sampledata-independent acquisitionuntargeted metabolite profilingcollision-induced dissociationVibrio harveyiThermo Scientific Q Exactive HFultra-performance liquid chromatography-mass spectrometryMetabolomicscentroid spectrumWaters ACQUITY UPLC H-Class Systemuntargeted analysisMassLynxQingdao Agricultural UniversityProgenesis QIWhole Organismexperimental sampledata-independent acquisitionuntargeted metabolite profilingcollision-induced dissociationVibrio harveyiThermo Scientific Q Exactive HF<p>Mass spectrometry analysis was performed using a high-resolution mass spectrometer equipped with an electrospray ionization (ESI) source. Data were acquired in both positive and negative ion modes. The scan range was m/z 50–1000, with typical source parameters set according to standard operation conditions.</p>2-Hydroxy-1,4-benzoquinone2-Furoate9,10-DHOMEN6,N6,O-Tridemethylpuromycin-5'-phosphateN-Acetyl-L-aspartate10beta,14beta-Dihydroxytaxa-4(20),11-dien-5alpha-yl acetateHydroxymethylbilane13,14-dihydro-16,16-difluoro Prostaglandin D22-deoxy-D-glucose 6-phosphateDeoxyribose 1-phosphateHydroxyatrazineLIPC 18:0;3Nitrous oxide(¡À)12,13-DiHOMEPS(16:1(9Z)/14:0)O-Succinyl-L-homoserineButanoylphosphateD-GlycerateGrayanotoxin IProstaglandin H2Avermectin A2b aglyconeCMP-2-aminoethylphosphonateMethanesulfonic acidScopoletinPC(16:0/0:0)Phytyl diphosphateSlaframineDimethyl-L-arginineLupinine7-cyano-7-carbaguanine3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione5-(3'-Carboxy-3'-oxopropyl)-4,6-dihydroxypicolinatePentahomomethionineL-Sorbosetunicamycin2-Acetamido-4-(D-alanylamino)-2,4,6-trideoxy-D-mannopyranose(2R)-O-Phospho-3-sulfolactateN-Citryl-spermidineKanamycin C5-Aminoimidazole ribonucleotide1,4-Decanolide6'-Oxokanamycin XPyrroline hydroxycarboxylic acidPE(17:0/14:0)KIRENOL5-Hydroxymethyl-2-furaldehydeHydrogenobyrinateAbscisic alcoholL-Olivosyl-oleandolide1D-Myo-inositol 1,4,5,6-tetrakisphosphate2-(3-Carboxy-3-(methylammonio)propyl)-L-histidinePG(14:0/16:0)TrihomomethionineGentiopicrinurea-1-carboxylic acid3alpha-Hydroxy-3,5-dihydromonacolin L acid6-Deoxyerythronolide B(9Z)-Octadecenoic acid5-HydroxylysineRed chlorophyll cataboliteNovamethymycin1-Palmitoyl Lysophosphatidic Acid(S)-cis-N-MethylstylopineEthyl methionateSodium gluconate2-Hydroxy-5-methylquinonePA(16:1(9Z)/0:0)3-Oxo-beta-iononePalmitaldehyde6''-O-Carbamoylkanamycin APE(16:0/0:0)7-Methylguanosine 5'-phosphate2-Oxo-4-methylthiobutanoic acidBlue pigmentGuanosineRibothymidine5'-(p-nitrophenyl)thioadenosinePE(14:0/18:1(11Z))1-(2-Aminophenyl)decane-1,3-dioneErucic acidD,L- gamma-hydroxyphosphinothricinCytochalasin BCDP-DG(18:1(11Z)/18:2(9Z,12Z))6-Thioxanthine 5'-monophosphatePI(16:1(9Z)/18:1(9Z))OctanalHexadecanoylcarnitine5,10-MethenyltetrahydromethanopterinAcetoacetic acidGlyceric acidalpha-1,5-L-Arabinotetraoseamiclenomycindelta-Octalactone(4S)-4-Hydroxy-5-phosphooxypentane-2,3-dione6-Hydroxytryprostatin BGentamicin A6'-OxolividamineGlutathionylaminopropylcadaverineN-Glycoloyl-neuraminatePC(o-22:1(13Z)/22:2(13Z,16Z))Ornithine1-Deoxy-11beta-hydroxypentalenateClavaminic acidMycolactone D2'-Deoxy-5-hydroxymethylcytidine-5'-triphosphateMycolactone FProstaglandin A2GlucobrassicinN1-Amidinostreptamine 6-phosphate6'-Oxo-G4182-PhosphinomethylmalateXTPHistidyltryptophyldiketopiperazine5'-[(3-aminopropyl)-amino]-5'-deoxyadenosine(3S)-3-Hydroxy-N6,N6,N6-trimethyl-L-lysine8,8a-DeoxyoleandolideL-Arginine phosphateFMNH4-(beta-Acetylaminoethyl)imidazole5-Amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracilGlycochenodeoxycholateDodecanoylcarnitine7(1)-Hydroxychlorophyll aUric acidDihydroneopterin triphosphate4'-O-DesmethylpapaveroxineAcitretinTacrolimusFumaric acidL-TyrosinePS(17:0/16:0)BaicaleinPC(14:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))Argininosuccinic acid3-Iodo-L-tyrosineMacrocin2'''-N-Acetyl-6'''-deamino-6'''-hydroxyneomycin CBenzoic acid5-methyl-2-furancarboxaldehyde(S)-5-Oxo-2,5-dihydrofuran-2-acetateL-PhenylalanineGinsenoside RcAntibiotic G-418Delphinidin 3-O-(coumaroylglucoside)6,8a-Seco-6,8a-deoxy-5-oxoavermectin ''1a'' aglyconeDIBOAO-Acetylserine2-(Formamido)-N1-(5-phospho-D-ribosyl)acetamidinebis-(2-methyl-3-furyl) disulfideDeamino-alpha-keto-demethylphosphinothricin15-keto IloprostXanomeline2-Pyrocatechuic acid(-)-Jasmonic acid1-Stearoyl-sn-glycerol 3-phosphocholineImmunomycin1-DeoxypentalenateS-Allyl-L-cysteine6,8-Dimethyl-6,7,8,9-tetradehydroergolineAcetyl-DL-LeucineD-Glyceraldehyde 3-phosphateCephaeline5-Fluorocyclohexadiene-cis,cis-1,2-diol-1-carboxylate4-Carboxy-2-hydroxy-6-methoxy-6-oxohexa-2,4-dienoateBacteriopheophytinQuinoneGibberellin A49,12,13-TriHOMEStreptidine 6-phosphate2-hydroxydeoxyadenosine 5'-triphosphateDelphinidin 3-O-glucoside3'-N-Debenzoyltaxolsn-glycerol-1-phosphatedXMPMelibiitolPC(16:1(9Z)/0:0)PS(16:0/18:1(11Z))DemecolcineAvermectin A2a monosaccharidePhosphoribosylformylglycineamidinePS(14:0/18:1(11Z))Paspalicine2-hydroxyhexadecanoic acid6-GingerolD-arabinono-1,4-lactoneGuaninezymosterol{[(2S,3S,4R)-2-amino-3,4-dihydroxyicosyl]oxy}phosphonic acidUDP-N-acetylmuraminate5-Phenyl-1,3-oxazinane-2,4-dionePI(16:1(9Z)/0:0)19-Oxoandrost-4-ene-3,17-dioneTetracenomycin D316-EpivellosimineCitric acidzymosterol intermediate 1b6-Phosphogluconic acidAdenosine 2'-phosphateNocardicin GPaspalinine4-Allyl-2,6-dimethyoxphenolHydroxymethylphosphonatebeta-TocotrienolNORETHINDRONE ACETATE(3S,4R)-3,4-Dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate5-iodo-2'-dUMPLPA(16:0/0:0)PG(18:1(11Z)/18:1(11Z))PikromycinBilirubinOrotateGlycocholateVolicitindITPItaconic acidNorajmaline2-nonanonePI(18:0/16:1(9Z))DG(16:1(9Z)/16:1(9Z)/0:0)PE(16:0/19:0)MirtazapineStachyosePI(16:0/18:1(9Z))CDP-ethanolaminebeta-PaxitriolOxytocinPC(o-16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))Discadenine1-Deoxy-D-xyluloseCytidinePG(18:1(9Z)/18:1(9Z))Carnitine1-Oleoyl-lysophosphatidic acidfructoselysine(5Z,8Z,11Z,14Z,17Z)-Icosapentaenoic acid1-Dehydro-[6]-gingerdioneItaconateAT-56DemethyllactenocinAgroclavine16-HydroxypalmitateAvermectin B1b monosaccharideSodium deoxycholate1-Stearoyl-2-oleoyl-sn-glycero-3-phosphoethanolamineCyanidin5-MethoxyindoleacetateGenisteinDihydrogranaticinPyrrolidonecarboxylic acidCholesterol 3-sulfateDodecanoic acidN-Acetyl-D-leucinePE(18:0/18:1(9Z))2-Carboxy-2,5-dihydro-5-oxofuran-2-acetateL-beta-Ethynylserine(5-Formylfuran-3-yl)methyl phosphateNystatin A1SarpagineLolitrem EKAPA(3S)-3-HydroxycyclocitralPS(18:0/18:1(9Z))13(S)-HODE3-Sulfino-L-alanine2-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate6'-Oxogentamicin X2Erythromycin CNeomethymycinAmphotericin B9,10-DiHOMESulfatePC(14:1(9Z)/16:1(9Z))Epothilone BEpothilone AGamma-Glu-LeuJanthitrem CGamma-GlutamylcysteinePS(19:0/18:1(11Z))Leukotriene F4GeldanamycinPG(16:0/0:0)[U](R)-Mevalonate 3-phosphateAvermectin A1b aglycone2-C-Methyl-D-erythritol 2,4-cyclodiphosphateD-Ribose 5-phosphate2-Hydroxyethanesulfonate3,4-dimethylenedioxy mandelic acidTartronate semialdehyde2-Oxo-2,3-dihydrofuran-5-acetateDemethylphylloquinolPrecorrin 6XCer 18:0;3/20:0;1Nystatin5-Hydroxy-1-(4-hydroxyphenyl)-3-decanoneMethylmalonateS-carbamylcysteine(-)-alpha-BisabololLaudanineSpiramycin 1CobinamideL-UrobilinPE(17:0/19:0)(25R)-3-Oxocholest-4-en-26-oatedTDP-L-megosamine1-[2,6,6-Trimethyl-1,3-cyclohexadien-1-yl]-2-buten-1-one¦Ä-CEHCGemfibrozilLysoPC(20:4(5Z,8Z,11Z,14Z))Glycerophospho-N-Palmitoyl Ethanolamine(3Z)-PhycoerythrobilinCandicidin D2-HydroxymuconateGabapentinRicinoleic acidPI(22:0/0:0)TetrahydropalmatinePC(o-18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))5-Amino-4-imidazolecarboxyamidePS(16:0/16:0)IPC 18:0;3/18:0;13-Oxo-3-ureidopropanoateFructose 1-phosphateLariciresinol14,15-Epoxyemindole SBL-TryptophanPS(16:1(9Z)/16:1(9Z))3-Hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oateWithaferin AGalactosylceramide (d18_1_18_0)GlycineamideribotidePC(12:0/16:1(9Z))Phthalate 3,4-cis-dihydrodiolAnetholeUracil5,6,7,8-tetrahydrofolyl-L-glutamic acidcoformycinIminoaspartic acid(3R,2'S)-Myxol 2'-alpha-L-fucoside[5-(Aminomethyl)furan-3-yl]methyl phosphatetaurocholic acid(S)-N-MethylcanadineNarbonolide3-Benzylmalic acidSphingosine 1-phosphatePG(16:1(9Z)/18:1(11Z))Demethylmacrocin21,22-Diprenylpaxilline(¡À)13-HpODECaprylic acidPI(16:1(9Z)/16:1(9Z))Butyl 2-methyl-2-propenoate2-Fluoropalmitic AcidDehydroascorbate2-Hydroxy-2,4-pentadienoateAlpha-Linolenic acidNovapikromycin3-Fluorocatechol2-thiophenemethanolXanthoxinGallic acid1,3-Dicaffeoylquinic acidFumitremorgin Adehydro-D-arabinono-1,4-lactoneSteviosideShikimate 3-phosphateDihydrozeatin-O-glucosideCMP-pseudaminic acidN-Octanoyl-L-homoserine lactoneCoumarin(7R)-7-(4-Carboxybutanamido)cephalosporanateGentamicin C1aAcetylvalerenolic acidbeta-D-GalactoseSolavetivoneHypoxanthine7,8-Dihydromethanopterin2,3-Dihydro-2,5-dihydoxy-6-methyl-4-H-pyran-4-one17-O-Acetylnorajmalinebeta-N-acetylneuraminateGluconolactoneL-ArginineCDP-DG(18:1(9Z)/18:0)dTDP-5-dimethyl-L-lyxoseAcrylic acidTiboloneCopal-8-ol diphosphateAscorbic acid 6-palmitatePE(16:0/17:0)(1S,4R)-1-Hydroxy-2-oxolimonenePC(16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))(1'S)-AverantinNicotinic acid mononucleotideImidazole acetol-phosphateAflatremAvideoxycholate8-[(1R,2R)-3-Oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate22-Oxodocosanoate2-Fluorocyclohexadiene-cis,cis-1,2-diol-1-carboxylateGibberellin A36Butirosin APC(10:0/16:0)D-Lactic acidp-BenzoquinoneL-Xylo-hexulonolactoneOleandolideO-beta-D-XylosylzeatinPA(18:0/18:1(9Z))Gamma-glutamyl-L-putrescinetryptophanhydroxamate5-O-beta-D-MycaminosyltylactoneProtorifamycin ICDP-DG(18:0/18:0)16-Feruloyloxypalmitate8-Methylthiooctyl glucosinolate9,10-Dihydroxystearate2-Maleylacetate(2S)-2-Phospholactatesn-Glycerol 3-phosphateChanoclavine-I aldehydeLividomycin BCDP-DG(18:1(9Z)/22:3(10Z,13Z,16Z))27-O-Demethyl-25-O-desacetylrifamycin SVglyceraldehyde-3-phosphateCellobioseAvermectin A2bUDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestanePS(14:1(9Z)/16:0)Avermectin B2b monosaccharideMegalomicin B3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestan-26-alQueuineAconitineUbiquinone Q1D-Ribitol 5-phosphatePGF1aethylphosphonatePhylloquinoneSphinganine 1-phosphateHesperidinTerpendole B4-Sulfolactone2,5-DihydroxybenzoateMyricetin 3-O-glucuronidePenitrem DPS(18:1(11Z)/18:1(11Z))4-FluoromuconolactoneN-Acetyl-DL-serinePA(18:1(9Z)/0:0)Pyroglutamic acidDL-2-hydroxy stearic acidcyclogutamateDiethylcarbamazineLuteolinSalicylic acidepsilon-Caprolactamcis-AcetylacrylateL-Acetylcarnitine3-Dimethylallyl-4-hydroxybenzoateXanthineGDP-D-glycero-alpha-D-manno-heptoseIPC 18:0;3/16:0;1PA(12:0/16:1(9Z))Gentamicin X2L-Pyrrolysine3-(3,4-Dihydroxyphenyl)pyruvateLysoPC(20:4(8Z,11Z,14Z,17Z))1-Palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamineCDP-DG(18:1(11Z)/20:4(8Z,11Z,14Z,17Z))1-octen-3-oneL-1-Pyrroline-3-hydroxy-5-carboxylate3-O-Mycarosylerythronolide B(4Z,7Z,10Z,13Z,16Z,19Z)-Docosahexaenoic acid2-MethyleneglutarateD-XylonolactonePI(12:0/16:1(9Z))N-Palmitoyl-L-serine phosphoric acidD-Glycerate 2-phosphateCostunolideOrotic acidMethylmalonic acid4,8-Dihydroxyquinoline3-Fluorocyclohexadiene-cis,cis-1,2-diol-1-carboxylate4-Cholesten-7alpha,12alpha-diol-3-oneFlavin adenine dinucleotide (FAD)Avermectin B2b aglyconeDocosanedioateCDP-DG(16:0/20:4(8Z,11Z,14Z,17Z))GlutathionylspermineUDP-N-acetyl-2-amino-2-deoxy-D-glucuronateNodularinPE(12:0/16:0)Tetradecanoyl-CoAAndrosterone sulfateAcacetinL-Serine-phosphoethanolaminePI(18:0/18:1(9Z))22alpha-Hydroxy-campesterolLolitriol(2S)-2-Hydroxy-3-oxobutyl phosphate¦Á-CEHCFestuclavine3-DehydroteasteroneSalbostatin 6'-phosphatePyruvaldehydeSedoheptulose5'-CMP6-Fluorocyclohexadiene-cis,cis-1,2-diol-1-carboxylateCDP-DG(18:0/16:0)Narbomycin5-HETEAnsamitocin P-3Stigmatellin A2-methyl-3-methylthiofuranO-Acetyl-L-homoserineGlucoiberverin6-Phosphonoglucono-D-lactoneAvermectin B2a3-Amino-2,3-dideoxy-scyllo-inososeN-hydroxy DAPCastavinol C2Uridine triphosphateCDP-DG(18:0/18:2(9Z,12Z))PS(17:0/19:0)4-guanidinobutanamide2-Chloro-3-oxoadipateLys Phe TyrSedoheptulose 7-phosphate13-Desoxypaxilline2-Carboxy-1,4-naphthoquinoneGlu Arg Phe AspSulfite5'-amino-5'-deoxyadenosineIrinotecan2-Hydroxymyristic Acid1-monohexadecanoylglycerolN-Phosphotaurocyaminebeta-D-Gentiobiosyl crocetinLysoPE(20_3(8Z,11Z,14Z)_0_0)Manumycin ACer 18:0;3/18:0;1HexadecanedioatefalseEffects of Scytosiphon lomentaria Polysaccharides on Vibrio harveyiThe findings suggest that Scytosiphon lomentaria can influence Vibrio harveyi activity and host response, providing potential applications for disease control.2026-05-152026-05-05MTBLS14431