MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/m_MTBLS14479_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/m_MTBLS14479_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/a_MTBLS14479_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/a_MTBLS14479_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/s_MTBLS14479.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14479<p>Metabolite identification was achieved by matching the accurate molecular mass, MS/MS fragmentation patterns and retention time of each metabolic feature with public metabolite databases including HMDB, Metlin and KEGG. Compound structural annotation was confirmed by comparing characteristic fragment ions with standard reference spectra, and metabolites were annotated at different confidence levels based on mass accuracy and spectral matching similarity.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed on an Agilent 1290 Infinity LC ultra-high performance liquid chromatography system for untargeted metabolomics profiling of poplar root samples.Parameter Value[Chromatography Instrument]: Agilent 1290 Infinity LCParameter Value[Autosampler model]: Agilent 1290 Infinity AutosamplerParameter Value[Column model]: Waters ACQUITY UPLC HSS T3 Column (1.8 μm, 2.1 mm × 100 mm)Parameter Value[Guard column]: Waters ACQUITY UPLC HSS T3 VanGuard Guard Column (1.8 μm, 2.1 mm × 5 mm)</p>Interaction between rhizobacterial community and host root metabolism influences poplar salt tolerance.Nanjing Forestry UniversityYangwenke Liaorootmass spectrometry assay<p>Frozen poplar root samples were ground into fine powder under liquid nitrogen. An appropriate amount of tissue powder was weighed, and metabolites were extracted using ice-cold methanol-water extraction solution. After thorough vortexing, ultrasonication and static extraction, the supernatant was collected by high-speed centrifugation. The obtained extract was filtered through a microporous membrane and subjected to UHPLC-MS untargeted metabolomics detection.</p>Populushttps://www.ebi.ac.uk/metabolights/MTBLS14479Yangwenke Liao. Nanjing Forestry University. liaoyangwenke@njfu.edu.cn.<p>Raw UHPLC-MS/MS data acquired by AB Sciex TripleTOF 6600 were imported into metabolomics professional software for baseline correction, peak alignment, peak picking, retention time calibration and normalization. Missing value filling, noise reduction and data standardization were performed to obtain consistent metabolic feature data matrices for subsequent qualitative and quantitative analysis.</p>Treatmentliaoyangwenke@njfu.edu.cn<p>Root tissues of Populus were carefully excavated, and adherent surface soil was rapidly removed with sterile distilled water. Fresh root samples were immediately frozen in liquid nitrogen after collection to quench metabolic activity, then transferred to a −80 °C ultra-low temperature refrigerator for long-term storage prior to untargeted metabolomics analysis.</p>MetabolomicsProteoWizard msconvertMetabolomicsuntargeted analysisrootexperimental blankPopulusAgilent 1290 Infinity HPLCAgilent 1290 Infinity LCAB SCIEX TripleTOF 6600untargeted metabolite profilingHenan UniversityProteoWizard msconvertMetabolomicsuntargeted analysisrootexperimental blankPopulusAgilent 1290 Infinity HPLCAgilent 1290 Infinity LCAB SCIEX TripleTOF 6600untargeted metabolite profilingHenan University<p>The UHPLC system was coupled with an AB Sciex TripleTOF 6600 high-resolution mass spectrometer for mass spectrometry acquisition.Parameter Value[Scan m/z range]: 50–1200 m/zParameter Value[Instrument]: AB Sciex TripleTOF 6600Parameter Value[Ion source]: Electrospray Ionization (ESI), positive and negative ion modesParameter Value[Mass analyzer]: Time-of-Flight (TOF) analyzer</p>Benzoic acidL-RhamnoseDihydroxyacetoneEmbelinN-Acetyl-L-phenylalanineSucrose3,4-Dihydroxyphenylacetic acidArachidic acidD-Quinovosesn-Glycerol 3-phosphoethanolaminePyrocatechol2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine(PC(16:0/18:1(9Z)))Palmitic acidOleanolic acid3'-O-methylcytidine4-Hydroxycinnamic acidAcetyl-DL-LeucineNorethindrone Acetate(+-)-TaxifolinScytaloneNervonic acidSuccinateGlycerol1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphateRibitolL-IditolPhenolProcyanidin B2D(-)-beta-hydroxy butyric acidMelezitose1,4-Dihydroxybenzene3-Dehydroshikimic acidalpha-ketoisovaleric acidGinkgolic AcidCholesteryl sulfateCyclohexylsulfamateL-ThreonateD-Arabinono-1,4-lactoneD-MannoseGentisic acidmyo-InositolOleic acidPrephenatebeta-Nicotinamide D-ribonucleotideShikimateDL-lactatePhloridzinSalicylic acidGalactinolL-ArabinosePantothenate5,6,7,8-tetrahydro-2-Naphthoic Acid1-Palmitoyl Lysophosphatidic AcidMaslinic Acid2-Dehydro-3-deoxy-D-gluconateD-Mannose 1-phosphatealpha-D-Galactose 1-phosphate2-Ethyl-2-Hydroxybutyric acid1-Palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamineHydroxyisocaproic acidGlycerol 3-phosphateDL-3-Phenyllactic acidVindolineD-Lyxose1,2-Benzenedicarboxylic acidCitrateThymidinePentadecanoic AcidGlucotropaeolinMethylmalonic acidNaringenin-7-O-GlucosideVanillic acidGlyceric acidStachyose1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphateUridine diphosphate glucose(UDP-D-Glucose)Gallic acid1-Oleoyl-sn-glycerol 3-phosphate1,3-Dicaffeoylquinic acidtrans-2-Hydroxycinnamic acidAlpha-D-Glucosecis-AconitateNname,cis-9,10-Epoxystearic acidL-Malic acid3-Hydorxy-3-methylglutaric acid3-Indolepropionic acidMyristic acidL-Gulonic gamma-lactoneL-GlutamateD-Biotin3R-hydroxy-butanoic acid2-Oxoadipic acidD-Threitol3-Hydroxyisovaleric acidDulcitol3,4-Dihydroxyhydrocinnamic acid(S)-2-HydroxyglutarateD-Glucono-1,5-lactoneRaffinoseTridecanoic acid (Tridecylic acid)L-CysteineTricosanoic acidUDP-N-acetylglucosamine3-Deoxy-2-keto-6-phosphogluconic acid2-Methyl-3-hydroxybutyric acidN-Acetyl-L-glutamatep-Hydroxycinnamaldehyde11-Keto-.beta.-boswellic acidAdenosine9-OxoODEAdenine13(S)-HODELinoleic acid2,3-Dihydroxybenzoic acidHomogentisic acidXylitolN-Acetylglucosamine 1-phosphateQuinateD-RiboseHeptadecanoic acidInosine3',5'-Cyclic guanosine monophosphateL-Aspartate6-Phospho-D-gluconatefalseInteraction between rhizobacterial community and host root metabolism influences poplar salt tolerance<p>Poplar seedlings were cultivated in saline soil with low salt concentrations for 120 days, after which root tissues were harvested for untargeted metabolomic analysis. For each genotype, roots of three to four seedlings were pooled to form one biological replicate, with four biological replicates established per genotype.</p>2026-05-152026-05-14MTBLS14479HMDB0006294HMDB0036458HMDB0000564/HMDB0007878/HMDB0007879/HMDB0007880/HMDB0007882/HMDB0007884/HMDB0007886/HMDB0007887/HMDB0007897/HMDB0007898/HMDB0007911/HMDB0007912/HMDB0007913/HMDB0007914/HMDB0007936/HMDB0007937/HMDB0007938/HMDB0007939/HMDB0007940/HMDB0007958/HMDB0007959/HMDB0007960/HMDB0007961/HMDB0007964/HMDB0007966/HMDB0007967/HMDB0007981/HMDB0007982/HMDB0007983/HMDB0007984/HMDB0007985/HMDB0008002/HMDB0008003/HMDB0008004/HMDB0008005/HMDB0008028/HMDB0008029/HMDB0008065/HMDB0008066/HMDB0008067/HMDB0008068/HMDB0008069/HMDB0008070/HMDB0008071/HMDB0008091/HMDB0008092/HMDB0008119/HMDB0008120/HMDB0008121/HMDB0008126/HMDB0008129/HMDB0008145/HMDB0008147/HMDB0008182/HMDB0008183/HMDB0008186/HMDB0008191/HMDB0008192/HMDB0008230/HMDB0008231/HMDB0008232/HMDB0008233/HMDB0008269/HMDB0008270/HMDB0008271/HMDB0008272/HMDB0008274/HMDB0008304/HMDB0008306/HMDB0008308/HMDB0008309/HMDB0008312/HMDB0008317/HMDB0008322/HMDB0008323/HMDB0008324/HMDB0008375/HMDB0008377/HMDB0008379/HMDB0008400/HMDB0008401/HMDB0008402/HMDB0008409/HMDB0008410/HMDB0008413/HMDB0008414/HMDB0008415/HMDB0008416/HMDB0008426/HMDB0008427/HMDB0008428/HMDB0008429/HMDB0008431/HMDB0008444/HMDB0008507/HMDB0008508/HMDB0008509/HMDB0008510/HMDB0008514/HMDB0008515/HMDB0008516/HMDB0008522/HMDB0008536/HMDB0008537/HMDB0008538/HMDB0008539/HMDB0008540/HMDB0008541/HMDB0008542/HMDB0008543/HMDB0008606/HMDB0008607/HMDB0008608/HMDB0008609/HMDB0008610/HMDB0008611/HMDB0008612/HMDB0008614/HMDB0008615/HMDB0008655/HMDB0008657/HMDB0008658/HMDB0008659/HMDB0008660/HMDB0008661/HMDB0008662/HMDB0008663/HMDB0008664/HMDB0008665/HMDB0008668/HMDB0008669/HMDB0008670/HMDB0008671/HMDB0008672/HMDB0008673/HMDB0008674/HMDB0008676/HMDB0008681/HMDB0008682/HMDB0008683/HMDB0008684/HMDB0008686/HMDB0008688/HMDB0008690/HMDB0008691/HMDB0008692/HMDB0008697/HMDB0008698/HMDB0008699/HMDB0008700/HMDB0008702/HMDB0008703/HMDB0008704/HMDB0008706/HMDB0008707/HMDB0008730/HMDB0008731/HMDB0008732/HMDB0008733/HMDB0008734/HMDB0008735/HMDB0008736/HMDB0008737/HMDB0008738/HMDB0008745/HMDB0008746/HMDB0008747/HMDB0008748/HMDB0008749/HMDB0008750/HMDB0008751/HMDB0008752/HMDB0008754/HMDB0008758/HMDB0008761/HMDB0008762/HMDB0008766/HMDB0008768/HMDB0008770/HMDB0008773/HMDB0008774/HMDB0008775/HMDB0008791/HMDB0008792/HMDB0008793/HMDB0008795/HMDB0008796/HMDB0008798/HMDB0008799/HMDB0008800HMDB0000905HMDB0000439HMDB0000354HMDB0004326HMDB0001392HMDB0000500HMDB0002040HMDB0006248HMDB0000201HMDB0000034HMDB0000050HMDB0001209HMDB0001043/HMDB0060102HMDB0029943HMDB0000043/HMDB0059606HMDB0001964/HMDB0003501HMDB0000876HMDB0003229HMDB0000630HMDB0000101HMDB0001401HMDB0002050HMDB0004983HMDB0000765HMDB0000169HMDB0001078HMDB0003411HMDB0000126HMDB0000086HMDB0030775HMDB0000738HMDB0000148HMDB0000673HMDB0000267/HMDB0000805/HMDB0060262HMDB0000279/HMDB0062698HMDB0035698HMDB0000215HMDB0002670HMDB0001488HMDB0000210HMDB0000816HMDB0001565HMDB0004610HMDB0000795HMDB0003213HMDB0003747HMDB0003572/HMDB0124941HMDB0001173HMDB0003553HMDB0000254HMDB0000258/HMDB0125600HMDB0012204HMDB0000933HMDB0032538HMDB0000301/HMDB0034174HMDB0001975HMDB0000225HMDB0001376HMDB0000355/HMDB0059737HMDB0000754HMDB0002035HMDB0001316HMDB0011756HMDB0000645HMDB0003345/HMDB0061922HMDB0000019HMDB0002212HMDB0001870HMDB0000229HMDB0000072HMDB0000094HMDB0000030HMDB0001882HMDB0000779/HMDB0014712HMDB0001311/HMDB0000190HMDB0006330HMDB0000283HMDB0004136HMDB0000107HMDB0005826HMDB0005807HMDB0000152HMDB0000139/HMDB0031818HMDB0000131HMDB0002259HMDB0000130HMDB0000624HMDB0000195HMDB0000646HMDB0000191HMDB0000574HMDB0003466HMDB0011632HMDB0000156HMDB0000849HMDB0000943/HMDB0062620HMDB0011730HMDB0000202HMDB0000211HMDB0000806HMDB0001367HMDB0001138HMDB0000512HMDB0002368HMDB0002364HMDB0000207HMDB0000220HMDB0000826HMDB0000228HMDB0012283HMDB0033973HMDB0003072HMDB0000508HMDB0000694HMDB0001895HMDB0003070HMDB0000114HMDB0000273HMDB0000910HMDB0000290HMDB0000286/HMDB0060079HMDB0000484HMDB0002917