MetDNA was used for differential metabolite identification. Metabolites were analyzed via OPLS-DA using SIMCA-P 14.1, with metabolites exhibiting VIP > 1 selected. These metabolites were then imported into MetaboAnalyst 6.0 for pathway analysis, ultimately yielding differential metabolic pathways.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["ACQUITY UPLC® BEH C18 column (100 × 2.1 mm, 1.7 μm), flow rate: 0.3 mL/min; autosampler temperature: 8℃; column temperature: 50℃; injection volume: 2 μL. The mobile phase consisted of 0.1% formic acid in acetonitrile (A) and 0.1% formic acid in water (B), with gradient elution.
"],"publication":["A Mechanistic Study on the Synergistic Antihypertensive Effects of Dendrobium officinale Compound Combined with Conventional Antihypertensive Drugs in Spontaneously Hypertensive Rats, Based on Metabolomics."],"submitter_name":["Cheng Tong"],"submitter_affiliation":["Linping Campus, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China"],"organism_part":["blood serum","feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Serum Sample Preparation: Serum samples (200 μL) were thawed, and 600 μL of methanol/acetonitrile (1:1, v/v) containing the internal standard L-2-chlorophenylalanine at a concentration of 10 μmol/L was added. The mixture was vortexed for 60 seconds, followed by centrifugation at 14,000 × g for 20 minutes at 4°C. The supernatant was transferred to a 1.5 mL centrifuge tube and subjected to freeze-drying. The dried sample residue was reconstituted with a solvent mixture consisting of acetonitrile/methanol (80:20, v/v) and ultrapure water in a 1:1 (v/v) ratio. The mixture was vortexed for 1–2 minutes, then centrifuged again at 14,000 × g for 20 minutes at 4°C. The resulting supernatant was transferred to an autosampler vial for analysis. A quality control (QC) sample, prepared by pooling equal aliquots from all study samples, was injected after every six analytical samples throughout the sequence.
Ileocecal content samples preparation from the ileocecal region: In this study, the ileocecal contents referred to are primarily those collected from the ileocecal junction. A 10 mg solid sample was excised and precisely weighed in a centrifuge tube. Subsequently, 20 μL of ultrapure water and 180 μL of acetonitrile/methanol (8:2, v/v) solution containing the internal standard L-2-chlorophenylalanine (at a concentration of 10 μmol/L) were added. The mixture was homogenized and then centrifuged at 14,000 rpm for 20 min at 4 °C. The resulting supernatant was transferred to a new centrifuge tube and lyophilized. The dried sample powder was reconstituted with a 1:1 (v/v) mixture of acetonitrile/methanol (80/20, v/v) and ultrapure water, vortexed for 1–2 min, and centrifuged again at 13,500 g for 20 min at 4 °C. The final supernatant was transferred into an autosampler vial for analysis. A quality control (QC) sample was analyzed after every six experimental samples to ensure analytical consistency.
"],"organism":["Wistar","wistar"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14513"],"author":["Cheng Tong. Linping Campus, The Second Affiliated Hospital of Zhejiang University School of Medicine. tongcc19981025@163.com."],"data_transformation_protocol":["Raw data were converted to mzXML format using MSConvert software. The mzXML data were imported into the XCMSplus offline workstation, where peak extraction was performed using the multigroup job function. MetDNA was used for differential metabolite identification.
"],"study_factor":["Treatment"],"submitter_email":["tongcc19981025@163.com"],"sample_collection_protocol":["The blood was allowed to stand at room temperature for 2 h, then centrifuged at 3,000 r/min for 10 min at 4 °C. Supernatants (200 μL) were aliquoted and stored at -80 °C for subsequent metabolomics analysis. Under sterile conditions, ileocecal contents were collected: one portion was immediately frozen in dry ice and sent to Meigu Gene Technology Co., Ltd. (Guangdong, China) for DNA extraction and sequencing; another portion was stored at -80 °C for later metabolomics analysis.
"],"omics_type":["Metabolomics"],"study_design":["Waters ACQUITY UPLC I-Class PLUS System","AB SCIEX TripleTOF 4621","AB SCIEX TripleTOF 4620","AB SCIEX TripleTOF 4629","AB SCIEX TripleTOF 4628","AB SCIEX TripleTOF 4627","AB SCIEX TripleTOF 4626","AB SCIEX TripleTOF 4625","AB SCIEX TripleTOF 4624","AB SCIEX TripleTOF 4623","AB SCIEX TripleTOF 4622","AB SCIEX TripleTOF 4619","AB SCIEX TripleTOF 4610","AB SCIEX TripleTOF 4695","AB SCIEX TripleTOF 4694","AB SCIEX TripleTOF 4693","AB SCIEX TripleTOF 4692","AB SCIEX TripleTOF 4691","Wistar","AB SCIEX TripleTOF 4618","AB SCIEX TripleTOF 4617","AB SCIEX TripleTOF 4616","AB SCIEX TripleTOF 4615","AB SCIEX TripleTOF 4614","AB SCIEX TripleTOF 4613","AB SCIEX TripleTOF 4612","AB SCIEX TripleTOF 4611","Metabolomics","AB SCIEX TripleTOF 4609","AB SCIEX TripleTOF 4608","AB SCIEX TripleTOF 4690","AB SCIEX TripleTOF 4687","AB SCIEX TripleTOF 4686","AB SCIEX TripleTOF 4685","AB SCIEX TripleTOF 4684","AB SCIEX TripleTOF 4683","AB SCIEX TripleTOF 4682","AB SCIEX TripleTOF 4681","AB SCIEX TripleTOF 4680","AB SCIEX TripleTOF 4607","AB SCIEX TripleTOF 4606","AB SCIEX TripleTOF 4605","AB SCIEX TripleTOF 4604","AB SCIEX TripleTOF 4603","AB SCIEX TripleTOF 4602","AB SCIEX TripleTOF 4689","AB SCIEX TripleTOF 4601","AB SCIEX TripleTOF 4688","AB SCIEX TripleTOF 4600","ProteoWizard msconvert","AB SCIEX TripleTOF 4676","AB SCIEX TripleTOF 4675","AB SCIEX TripleTOF 4674","AB SCIEX TripleTOF 4673","AB SCIEX TripleTOF 4672","AB SCIEX TripleTOF 4671","AB SCIEX TripleTOF 4670","AB SCIEX TripleTOF 4679","AB SCIEX TripleTOF 4678","AB SCIEX TripleTOF 4677","AB SCIEX TripleTOF 4665","AB SCIEX TripleTOF 4664","AB SCIEX TripleTOF 4663","AB SCIEX TripleTOF 4662","AB SCIEX TripleTOF 4661","AB SCIEX TripleTOF 4660","AB SCIEX TripleTOF 4669","AB SCIEX TripleTOF 4668","AB SCIEX TripleTOF 4667","AB SCIEX TripleTOF 4666","AB SCIEX TripleTOF 4654","AB SCIEX TripleTOF 4653","AB SCIEX TripleTOF 4652","AB SCIEX TripleTOF 4651","AB SCIEX TripleTOF 4650","blood serum","AB SCIEX TripleTOF 4659","AB SCIEX TripleTOF 4658","AB SCIEX TripleTOF 4657","AB SCIEX TripleTOF 4656","AB SCIEX TripleTOF 4655","untargeted analysis","AB SCIEX TripleTOF 4643","AB SCIEX TripleTOF 4642","AB SCIEX TripleTOF 4641","AB SCIEX TripleTOF 4640","AB SCIEX TripleTOF 4649","AB SCIEX TripleTOF 4648","AB SCIEX TripleTOF 4647","AB SCIEX TripleTOF 4646","AB SCIEX TripleTOF 4645","AB SCIEX TripleTOF 4644","experimental sample","AB SCIEX TripleTOF 4632","AB SCIEX TripleTOF 4631","AB SCIEX TripleTOF 4630","XCMS","AB SCIEX TripleTOF 4639","AB SCIEX TripleTOF 4638","AB SCIEX TripleTOF 4637","AB SCIEX TripleTOF 4636","AB SCIEX TripleTOF 4635","hypertension","feces","AB SCIEX TripleTOF 4634","AB SCIEX TripleTOF 4633"],"curator_keywords":["Waters ACQUITY UPLC I-Class PLUS System","AB SCIEX TripleTOF 4621","AB SCIEX TripleTOF 4620","AB SCIEX TripleTOF 4629","AB SCIEX TripleTOF 4628","AB SCIEX TripleTOF 4627","AB SCIEX TripleTOF 4626","AB SCIEX TripleTOF 4625","AB SCIEX TripleTOF 4624","AB SCIEX TripleTOF 4623","AB SCIEX TripleTOF 4622","AB SCIEX TripleTOF 4619","AB SCIEX TripleTOF 4610","AB SCIEX TripleTOF 4695","AB SCIEX TripleTOF 4694","AB SCIEX TripleTOF 4693","AB SCIEX TripleTOF 4692","AB SCIEX TripleTOF 4691","Wistar","AB SCIEX TripleTOF 4618","AB SCIEX TripleTOF 4617","AB SCIEX TripleTOF 4616","AB SCIEX TripleTOF 4615","AB SCIEX TripleTOF 4614","AB SCIEX TripleTOF 4613","AB SCIEX TripleTOF 4612","AB SCIEX TripleTOF 4611","Metabolomics","AB SCIEX TripleTOF 4609","AB SCIEX TripleTOF 4608","AB SCIEX TripleTOF 4690","AB SCIEX TripleTOF 4687","AB SCIEX TripleTOF 4686","AB SCIEX TripleTOF 4685","AB SCIEX TripleTOF 4684","AB SCIEX TripleTOF 4683","AB SCIEX TripleTOF 4682","AB SCIEX TripleTOF 4681","AB SCIEX TripleTOF 4680","AB SCIEX TripleTOF 4607","AB SCIEX TripleTOF 4606","AB SCIEX TripleTOF 4605","AB SCIEX TripleTOF 4604","AB SCIEX TripleTOF 4603","AB SCIEX TripleTOF 4602","AB SCIEX TripleTOF 4689","AB SCIEX TripleTOF 4601","AB SCIEX TripleTOF 4688","AB SCIEX TripleTOF 4600","ProteoWizard msconvert","AB SCIEX TripleTOF 4676","AB SCIEX TripleTOF 4675","AB SCIEX TripleTOF 4674","AB SCIEX TripleTOF 4673","AB SCIEX TripleTOF 4672","AB SCIEX TripleTOF 4671","AB SCIEX TripleTOF 4670","AB SCIEX TripleTOF 4679","AB SCIEX TripleTOF 4678","AB SCIEX TripleTOF 4677","AB SCIEX TripleTOF 4665","AB SCIEX TripleTOF 4664","AB SCIEX TripleTOF 4663","AB SCIEX TripleTOF 4662","AB SCIEX TripleTOF 4661","AB SCIEX TripleTOF 4660","AB SCIEX TripleTOF 4669","AB SCIEX TripleTOF 4668","AB SCIEX TripleTOF 4667","AB SCIEX TripleTOF 4666","AB SCIEX TripleTOF 4654","AB SCIEX TripleTOF 4653","AB SCIEX TripleTOF 4652","AB SCIEX TripleTOF 4651","AB SCIEX TripleTOF 4650","blood serum","AB SCIEX TripleTOF 4659","AB SCIEX TripleTOF 4658","AB SCIEX TripleTOF 4657","AB SCIEX TripleTOF 4656","AB SCIEX TripleTOF 4655","untargeted analysis","AB SCIEX TripleTOF 4643","AB SCIEX TripleTOF 4642","AB SCIEX TripleTOF 4641","AB SCIEX TripleTOF 4640","AB SCIEX TripleTOF 4649","AB SCIEX TripleTOF 4648","AB SCIEX TripleTOF 4647","AB SCIEX TripleTOF 4646","AB SCIEX TripleTOF 4645","AB SCIEX TripleTOF 4644","experimental sample","AB SCIEX TripleTOF 4632","AB SCIEX TripleTOF 4631","AB SCIEX TripleTOF 4630","XCMS","AB SCIEX TripleTOF 4639","AB SCIEX TripleTOF 4638","AB SCIEX TripleTOF 4637","AB SCIEX TripleTOF 4636","AB SCIEX TripleTOF 4635","hypertension","feces","AB SCIEX TripleTOF 4634","AB SCIEX TripleTOF 4633"],"mass_spectrometry_protocol":["A Waters quadrupole time-of-flight mass spectrometer (Q-TOF/MS) was employed. The time-of-flight mass spectrometer was equipped with a TurboIonSpray ion source and operated in both positive and negative electrospray ionization (ESI) modes. Specific parameters were as follows: nebulizer gas (GS1) and heater gas (GS2) were set to 55 psi, curtain gas (CUR) was maintained at 35 psi, ion source temperature was 600 °C, and the ion spray voltage (ISVF) was set to +5500 V for positive mode and -4500 V for negative mode. The TOF/MS scan range was from m/z 50 to 1000 Da.
"],"additional_accession":[]},"is_claimable":false,"name":"A Mechanistic Study on the Synergistic Antihypertensive Effects of Dendrobium officinale Compound Combined with Conventional Antihypertensive Drugs in Spontaneously Hypertensive Rats, Based on Metabolomics","description":"This dataset comprises metabolomic profiling data of serum and gut metabolites from 6 female Wistar rats and 42 female spontaneously hypertensive rats (SHR). Six Wistar rats were assigned to the normal control group (Normal), and 42 SHR rats were randomly divided into seven groups with six rats in each group: model group (Model), low-dose Dendrobium mixture group (LF), medium-dose Dendrobium mixture group (MF), high-dose Dendrobium mixture group (HF), irbesartan group (IRB), dual antihypertensive drug combination group (IAC), and herbal formula combined with dual antihypertensive drugs group (FIAC). The gavage volume for all groups was 2 mL/100 g body weight, administered once daily for six consecutive weeks. Rats in the LF, MF, and HF groups received the crude herbal formula at doses of 0.7 g/kg, 1.4 g/kg, and 2.8 g/kg, respectively. The IRB group received irbesartan at 15 mg/kg, the IAC group received a combination of irbesartan (15 mg/kg) and amlodipine (0.5 mg/kg), and the FIAC group received the herbal formula (2.8 g/kg) plus irbesartan (15 mg/kg) and amlodipine (0.5 mg/kg). The Normal and Model groups were administered an equivalent volume of physiological saline.","dates":{"publication":"2026-05-18","submission":"2026-05-18"},"accession":"MTBLS14513","cross_references":{}}