MetaboLights

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data were processed and annotated according to previously published methods (Fuhrer et al., 2011). Missing values were imputed using a recursive approach in the raw data. Upon identification of consensus centroids across all samples, ions were putatively annotated by accurate mass and isotopic patterns. Starting from the HMDB v4.0 database, we generated a list of expected ions including deprotonated, fluorinated, and all major adducts found under these conditions. All formulas matching the measured mass within a mass tolerance of 0.001 Da were enumerated. As this method does not employ chromatographic separation or in-depth MS2 characterization, it is not possible to distinguish between compounds with identical molecular formula. The confidence of the annotation reflects Level 4.MetaboLightsPublicFlow injection analysis MS - negativeParalleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment. 10.64898/2026.01.29.702624.Glass bead-based microcosms enable simultaneous profiling of root exudate and microbiome dynamics. 10.1016/j.rhisph.2026.101363.PMI, University of BaselUniversity of BaselJan WaelchliCharlotte JollerMetabolomics analysis was performed using flow injection-time of flight mass spectrometry (FIA) on a platform consisting of an Agilent 1200 pump coupled to a Gerstel MPS2 autosampler and an Agilent 6550 QTOF mass spectrometer (Agilent, Santa Clara, USA) operating in negative ionisation mode with published parameters (Fuhrer et al., 2011). Briefly, a mobile phase of isopropanol:water 60:40% (v/v) buffered with 5 mM ammonium fluoride was supplied at a rate of 150 µL min-1. Homoserine and Hexakis (1H, 1H, 3H-tetrafluoropropoxy)-phosphazine were added to the mobile phase for online mass axis correction. Mass spectra ranging within 50-1000 m/z were recorded at a frequency of 1.4 spectra s-1 using maximum resolving power. The source temperature was set to 325 °C, with 5 L min-1 drying gas and a nebulizer pressure of 30 spig. Fragmentor, skimmer, and octupole voltages were set to 175 V, 65 V, and 750 V, respectively. Technical blanks (methanol: ultrapure water 1:1 and 20 mM ammonium acetate), sample pools and a standard mixture of 1 µM amino acids were used as quality controls (QC). Samples were injected twice in random order with blank injections between sample groups (QC, exudates, tissues) to reduce sample carryover. The mean of replicated sample measures was taken for data analysis.standardblankrootshootroot exudatemass spectrometry assayBefore metabolomics analysis, exudates were gently thawed on ice and diluted according to root fresh weights (McLaughlin et al., 2023).Frozen roots and shoots were ground to a powder in liquid nitrogen and metabolites were extracted twice on ice in methanol:acetonitrile:ultrapure water 2:2:1 (v/v) for 1 h with intermittent vortexing. After centrifugation at 10’000 g and 4 °C for 5 min, supernatants of both extraction rounds were pooled and dried under vacuum. For metabolite analysis, dried extracts were resuspended in methanol:ultrapure water 1:1 (v/v) according to the tissue fresh weight (200 µL per 70 mg of tissue) and diluted 150 times (McLaughlin et al., 2023).standardArabidopsis thalianablankhttps://www.ebi.ac.uk/metabolights/MTBLS14526Jan Waelchli. University of Basel. jan.waelchli@unibas.ch.Nicola Zamboni. ETH Zurich. nzamboni@ethz.ch.Charlotte Joller. University of Basel. charlotte.joller@unibas.ch.Joelle Sasse. University of Zurich. joelle.schlaepfer@agroscope.admin.ch.Klaus Schlaeppi. University of Basel. klaus.schlaeppi@unibas.ch.Analysis of FIA data was carried out in R v4.4.2 (R Core Team, 2024). To remove metabolite background noise, FIA data were filtered against experimental blanks (blank glass-microcosms and blank Petri dishes for exudates), technical blanks (methanol:ultrapure water 1:1 for tissues). Metabolites were excluded as background if their median intensities in exudate samples were not at least twice as high as the median intensities in blanks. Sample intensities were normalised using Probabilistic Quotient Normalisation (PQN) (Xia and Wishart, 2011) and for multivariate analysis, metabolites were scaled using autoscaling (van den Berg et al., 2006).TreatmentTimepointjan.waelchli@unibas.chcharlotte.joller@live.deSeedlings were pricked to fresh 0.5 strength MS-Agar plates after one week of growth under controlled conditions in a plant growth chamber (Percival, CFL Plant Climatics, Wertingen, Germany) set to short days with 10 h light (220 µmol m-2 s-1) (21 °C), and 14 h darkness (18 °C) and grown for two more weeks before being transferred to microcosms. Glass-microcosms were prepared as described (McLaughlin et al., 2023). Briefly, 150 mL of glass beads (5 mm) were added to 850 mL glass jars (5743922, Weck glass and packaging GmbH, Bonn, Germany), autoclaved and amended with 35 mL of liquid 0.5 MS. Three plants were added per microcosm. Microcosms were sealed with glass lids and micropore tape. Note that air exchange was enhanced by using micropore tape to introduce a space between the lid and the jar (McLaughlin et al., 2023).Half the microcosms were inoculated with a synthetic bacterial community composed of 9 strains (SynCom9) one day after the transfer to microcosms. The inoculum for microcosms was prepared as described (McLaughlin et al., 2023). Briefly, single colonies were picked to prepare dense suspensions in 800 µL of 10 mM MgCl2. Strain suspensions were washed three times in 10 mM MgCl2 and mixed in equal volumes. This mix was adjusted to a final OD600 of 0.002 in 0.5 strength MS, which was used as the substrate solution in microcosms.Plants were cultivated in microcosms for 13 days.Exudates were collected in a time series at 0, 1, 2, 4 and 12 days after SynCom9 inoculation under axenic conditions. Exudates were harvested by replacing the growth medium with 35 mL exudate collection solution equimolar to 0.5 MS (20 mM sterile-filtered ammonium acetate) for 2 h. The same procedure was followed with unplanted microcosms to obtain experimental blank samples. Tissues of single plants within one microcosm were separated after exudate harvest to use for SynCom9 and metabolite profiling.MetabolomicsstandardMetabolomicsblankbacteriaFlow Injection Analysisuntargeted analysisPlant Exudatesmicrobiomeroot exudateuntargeted metabolite profilingArabidopsis thalianaAgilentAgilent 6550 iFunnel Q-TOFplantrootTime Seriessynthetic microbial communityshootexperimental blankstandardMetabolomicsblankbacteriaFlow Injection Analysisuntargeted analysisPlant Exudatesmicrobiomeroot exudateuntargeted metabolite profilingArabidopsis thalianaAgilentAgilent 6550 iFunnel Q-TOFplantrootTime Seriessynthetic microbial communityshootexperimental blankMetabolomics analysis was performed using flow injection-time of flight mass spectrometry (FIA) on a platform consisting of an Agilent 1200 pump coupled to a Gerstel MPS2 autosampler and an Agilent 6550 QTOF mass spectrometer (Agilent, Santa Clara, USA) operating in negative ionisation mode with published parameters (Fuhrer et al., 2011). Briefly, a mobile phase of isopropanol:water 60:40% (v/v) buffered with 5 mM ammonium fluoride was supplied at a rate of 150 µL min-1. Homoserine and Hexakis (1H, 1H, 3H-tetrafluoropropoxy)-phosphazine were added to the mobile phase for online mass axis correction. Mass spectra ranging within 50-1000 m/z were recorded at a frequency of 1.4 spectra s-1 using maximum resolving power. The source temperature was set to 325 °C, with 5 L min-1 drying gas and a nebulizer pressure of 30 spig. Fragmentor, skimmer, and octupole voltages were set to 175 V, 65 V, and 750 V, respectively. Technical blanks (methanol: ultrapure water 1:1 and 20 mM ammonium acetate), sample pools and a standard mixture of 1 µM amino acids were used as quality controls (QC). Samples were injected twice in random order with blank injections between sample groups (QC, exudates, tissues) to reduce sample carryover. The mean of replicated sample measures was taken for data analysis.cis-11-Methyl-2-dodecenoic acidPaeoniflorigenoneStaurosporineProstaglandin G2L-CapreomycidineCorilagin5-O-Methyl-myo-inositolBenzyl 2-methyl-3-oxobutanoate2',3'-Cyclic UMPDihydroxyfumarateButynolEctoineCycloateCoumestrolFormyl-AspPantothenolKinetinde-Hypoxanthine futalosineN-Nitrosoproline3-Hydroxy-3-(3-hydroxy-4-methoxyphenyl)propionyl-CoADihydrokaempferolSterol 3-beta-D-glucosideAllicinalpha-(p-Methoxyphenyl)-6-methyl-2-pyridineacrylic acidMethanesulfonic acidCarpipramine maleateCitrullineScopoletinGlycolateHispidolQuercetin 3-O-glucosideCastasteronePolypodine BBenzoate1,2-Didodecanoyl-sn-glycero-3-cytidine-5'-diphosphate(1-Hydroxycyclohexan-1-yl)acetyl-CoAN-((R)-Pantothenoyl)-L-cysteine(2S,3S)-2-Hydroxytridecane-1,2,3-tricarboxylateGallocatechin-(4alpha->8)-epigallocatechinN-Acetyl-D-tryptophan2-Amino-3,7-dideoxy-D-threo-hept-6-ulosonic acidDecursin2-Amino-9,10-epoxy-8-oxodecanoic acidFurfuralO-ButanoylcarnitineVermeloneHaplopineIlexolide ACatharineLusitanicosideArginineRicinine4-Methylene-L-glutamineDeamino-NAD+HydroxybutynalC13:0Toxoflavine6-DeoxocastasteroneN-MethylhexanamideGlutathioneGlucohirsutin7-DehydrodesmosterolGnidicin3-Hydroxy-2-methylpyridine-4,5-dicarboxylateChrysoeriol13-Apo-beta-carotenone4-O-(beta-L-Arabinofuranosyl)-(2S,4S)-4-hydroxyproline1,2-Bis(4-nitrophenyl)ethaneGentiopicrin4-Amino-2-methyl-5-phosphomethylpyrimidineall-trans-Heptaprenyl diphosphate4,4'-SulfonyldiphenolAminoadipate7,8-DiaminononanoateIsophorone2-Hexaprenyl-3-methyl-6-methoxy-1,4-benzoquinonedTDP-D-mycaminose(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylateC24:1C24:06''-O-Carbamoylkanamycin AViolanthinLevetiracetam2-Aminoethylphosphonate1,2-Bis(4-hydroxy-3-methoxyphenyl)ethyleneHexose oximeErucic acidcis-1,2-Dihydro-3-ethylcatecholGlycerophosphoglycerolRobinin6-Thioxanthine 5'-monophosphateSaccharin3-(Acetyloxy)-9-mercaptoandrosta-3,5-diene-11,17-dioneN-AcetyllactosamineSoyasaponin IIDimethisteronePiperidione2-Hydroxymethylserine8-Amino-7-oxononanoateL-AlbizziineMaleimideIBMXColubrinMupirocinHelveticosideBenzyl alcoholGlutathionylaminopropylcadaverineLevofuraltadone3alpha,12alpha-Dihydroxy-5beta-pregnan-20-one diacetateSinapoyltartronate7-(Acetyloxy)-3-(3-pyridinyl)-2H-1-benzopyran-2-onePaeonolideScymnolChloropropanoic acidL-Selenocystathionine1-Dodecanoyl-sn-glycerolN3'-AcetylapramycinOrnithineEugeniinTetrodotoxinAndrosterone glucuronideIsovalerylcarnitineSerinedTDP-6-deoxy-beta-L-taloseFusidic acidParamethadioneHC Blue No. 2Cholylglycine2-PhosphoglycolateRutaecarpinedTDP-4-dimethylamino-4,6-dideoxy-5-C-methyl-L-mannoseBenzamideTrichodermin4-(beta-D-Glucosyloxy)benzoateAcrolein2-PhosphinomethylmalateSodium folinate2,3-Dihydroxycyclopentaneundecanoic acidC15:1C15:0N-[4-(5-Nitro-2-furyl)-2-thiazolyl]acetamide8,8a-Deoxyoleandolide4-Methyl-2-oxopentanoateFraxetinD-Lysopine6-Hydroxypseudooxynicotine2-C-Methyl-D-erythritol 4-phosphateAdenosineAbyssinone Ialpha-Tocopherol acetateAndrost-4-ene-3beta,17beta-diol diacetateProstaglandin B1CyasteroneIndolepyruvate3-Dehydroshikimate4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-olPiceidMethomylButabarbitalHexonic acidNeolinustatinOligomycin COligomycin AFenuron-TCAD-Erythrose 4-phosphate2,3-Dinor-8-iso prostaglandin F2alphaMethyleugenolAspartate semialdehydeN-AcetylneuraminatePeimine1,3-BenzenedisulfonamidePurine mononucleotide2-Chloro-cis,cis-muconatedCDPC28:3CandesartanStearidonic acid(+)-PinoresinolC28:111-O-Demethylpradinone IIArgatrobanSisomicinChinomethionatTraumatic acid1-Naphthalenesulfonic acid2-Hydroxy-6-oxo-6-(2-hydroxyphenoxy)-hexa-2,4-dienoateGlutathione episulfonium ion1-Hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate10-DeoxymethynolideHellebrigenin 3-acetatePectenotoxin 1Pseudoargiopinin III(-)-Jasmonic acidSphingosine(Ac)2-L-Lys-D-AlaResazurinGlucoraphaninTrisaccharidesn-Glycero-3-phosphoethanolamineCoryneine2-Hydroxybenzo[a]pyreneTetrahydrocortisoneThreonineThien-2-ylacetate9,12,13-TriHOME2-Thiophenesulfonamide(1-Ribosylimidazole)-4-acetateN-Acetyl-D-fucosamineHR1917Agrimol CMusarosidedelta-Guanidinovaleric acidS-(2-Hydroxyethyl)-N-acetyl-L-cysteineDithianon3-NitrosothiazolidinePhenylethylmalonamideGlucono-lactone2,3-DimethylmaleatePhenylacetaldehydeLythidathionAzinphos-methylSuccinic anhydrideUmbelliferone2-Quinolinecarboxylic acid1-O-Vanilloyl-beta-D-glucose1-O-Hexadecyl-sn-glycerol4-MaleylacetoacetateTS-TM-calix(4)arene4-HydroxyphenylacetateGuanine16alpha,17beta-Dihydroxyandrost-4-en-3-one dipropionatetrans,octacis-Decaprenylphospho-beta-D-ribofuranose 5-phosphateEthyl 3-(N-butylacetamido)propionate(5Z,13E)-11alpha-Hydroxy-9,15-dioxoprost-13-enoate7,8-DihydroxykynurenateProtoporphyrinogen IXCyclic dehypoxanthine futalosineN-Heptanoylhomoserine lactone5-O-Feruloylquinic acid3,5-Dihydroxy-1,4-naphthoquinone3-(4-Methylpent-3-en-1-yl)pent-2-enedioyl-CoAHydroxypyruvateL-ArogenateIndole-3-acetaldoxime N-oxideO-PropanoylcarnitineHydroxymethylphosphonate4-MethylthiobutylthiohydroximateAbietateHeritonin4-O-(beta-L-Arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyprolineHexuronic acid; AscorbateSterolGyromitrinMaleamic acidArgiotoxin 6592-HydroxyiminostilbeneCefotaximeVeratraminePikromycinUDP-L-Ara4N(-)-SympatolDiginatinO-Phospho-4-hydroxy-L-threonineChenodeoxycholateVolicitinProtopanaxadiolDemethylphosphinothricinFuroic acidN-AcetylimidazoleHexose PIbuprofen(8Z,11Z,14Z)-Icosatrienoic acidAquayamycinGlutamyl-glutamic acidN-Adenylyl-L-phenylalanine9-Oxononanoic acidTyr-OEtDibutyl adipate4-HexyloxyphenolLatia luciferinOrnalineGlutamateCarnitineTetrose(+/-)-6-Acetonyldihydrosanguinarine2-Oxo-7-methylthioheptanoic acid2-Amino-5-phosphopentanoic acidHexazinoneBenzofuranCucurbic acid5-Guanidino-3-methyl-2-oxopentanoate(2R,3R)-3-Methylglutamyl-5-semialdehyde-N6-lysineML-236CMangiferinUrate D-ribonucleotideAsiatic acidAkeboside SteDihydroclavaminic acidVanillyl alcoholSarcoaldesterol ADihydroxy-Val16-HydroxypalmitateFerroxamineGibberellin A12 aldehydePropylthiouracilEchinenoneN-NitrosodiphenylamineLycoflexine2-Oxooctadecanoic acidGlyoxylic acid1,3-Dimethyl-8-isoquinolinolTiagabineManniflavanoneThiobenzamide S-oxideNuatigeninL-Adrenaline7alpha-HydroxycholesterolOleoylglycerone phosphateAminoalanineDehydrojuvabioneBrusatolGraphinone3-KetosphingosineC19:013,16,19-Docosatrienoic acidGlucoalyssinIndolelactateThymineFarrerolFagaramide5-Amino-4-chloro-2-(5-hydroxymuconoyl)-3(2H)-pyridazinoneThiodiacetic acidMucronine ACalcidiolDihydrolipoamideLevomethadyl acetateMucronine BErgocristineKikkanol Abeta-Alanyl-L-lysine4-Amino-1-piperidinecarboxylic acidTaurineEpothilone DPhaseolic acidMancinellinAmastatinDiisopropyl phthalateGeldanamycin1-(3,4-Dihydroxyphenyl)-5-hydroxy-3-decanoneLithocholic acidC9:1Succinic aldehydeOsladin3-MercaptolactateDanielone5-L-Glutamyl-taurineVerruculogenPyridoxal16alpha-HydroxydehydroepiandrosteroneMethyl 2-diazoacetamidohexonate(10S)-Juvenile hormone III diolFalcarindiolAcetohexamideImperialineCefsulodin sodiumGlycerolN-Acetyl-D-glucosaminePotassium 2,6-dihydroxytriazinecarboxylateCatecholOxoglutarateDanazol2-Dehydro-D-xylonateOxaloacetic acidPhytuberinPrometonUbiquinone-6IsoscoparineHordatine B3-(4-Hydroxyphenyl)lactateHordatine AGlycyrrhetaldehydeAcetyl-LeuClocapramine dihydrochlorideSuccinimideUndecanoic acidCyclo[(Z)-alpha,beta-didehydrophenylalanyl-L-leucyl]dTDP-hexose1-O-Sinapoyl-beta-D-glucoseCalcitriolPhospho-anandamidePropenoic acid C3:1Officinalisnin IJuvenile hormone II acidTrabectedinCoumarate5alpha-CyprinolStylisterol BAnthemis glycoside B3-Methyleneoxindole5-MethylthiopentanaldoximeElaeokanine C1-Guanidino-1-deoxy-scyllo-inositol 4-phosphatePentonic acidN-DesmethylzolmitriptanBenzoylagmatinePahutoxin6-Pentadecylsalicylic acidDopaminePhenylalanine(-)-AcanthocarpanN-Acetylgalactosaminate(5-L-Glutamyl)-L-glutamineBrevianamide F4-MethoxyglucobrassicinNiridazoleDihydrolipoateBruceoside A6-DeoxoteasteronedCMPStovaine2-Oxo-4-phosphonobutanoate2,3,9,10-Tetrahydroxyberbine7-MethylthioheptanaldoximePantothenateTestosteroneFurazolidonePsoralidinSoyasapogenol BOxobutanoic acidGitogeninCDP-glycerolIsoorientin 2''-O-rhamnosideD-Glucono-1,5-lactone 6-phosphateAconitateUracilDiacetylhydrazineRheinAplysinN-(3-Oxooctanoyl)homoserine lactoneN-CaffeoylputrescineN-Sulfo-D-glucosamineAcetyl-PheAminopentanamideStipitatonate(R)-3-((R)-3-Hydroxybutanoyloxy)butanoateBakkenolide A5-Aminolevulinic acidHydrocortisone caproate(6S,9R)-VomifoliolCholesterol sulfateCytosineCysCentarolPentanoic acid(-)-Menthyl O-beta-D-glucosideGDP-hexoseEthosuximideL-CystineNitrosopiperidineCobalt-precorrin 3ErythromycinParathion-methylN-Hydroxy-MeIQxFalcarinoneBufotenidineAralionine AS-(4-Bromophenyl)-L-cysteine1-Hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine3-Hexaprenyl-4,5-dihydroxybenzoateMalateOxalic acidCobalt-precorrin 7(15S)-15-Hydroxy-5,8,11-cis-13-trans-eicosatetraenoateN-Phosphohypotaurocyamine2,3-Dihydroxybenzoate4-[2-(5-Carboxy-2-hydroxy-3-methoxyphenyl)-2-oxoethylidene]-2-hydroxy-2-pentenedioatecis-1,2-Dihydronaphthalene-1,2-diol2-Hydroxycyclohexan-1-oneGeranyl formate20-O-Methyl-19-chloroproansamitocinPremithramycin A3'2-OxoglutaramateHeptadecatrienoic acid6beta-Hydroxy-D-homo-17a-oxaandrost-4-ene-3,17-dioneJatrophatrionep-CoumaroylagmatineDiosminIndole-3-acetateMethylazoxymethanol3-Hydroxy-palmitic acid methyl esterTriptolideSapelin ACalystegin B2Hydroxy-valineAminophenolPhytanateBepridilCholesteryl-beta-D-glucosidePhensuximideTheogallin3,7-Di-O-methylquercetinEudistomin CC4:0 (Butyric acid)1'-Acetoxyeugenol acetatePyruvate4-Chloro-alpha-[4-[2-(diethylamino)ethoxy]phenyl]-alpha-phenylbenzeneethanolAminomethylurea(9R,10R)-Dihydroxyoctadecanoic acidMethyl-LysNaphthalene-1,2-diolAnthranilatealpha-TocotrienolUDP-ketopentoseKrm 1657Tuberonic acid glucosideDigitalinCyclic ADP-ribose5-Hydroxyferulic acid methyl esterGlutamineTerpendole GTerpendole IDihydropinosylvinBenzosemiquinone3beta-Hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylateFurcatinHarzianopyridonesn-Glycerol 3-phosphateSpicamycin2-Hydroxycyclohexane-1-carboxyl-CoAHistidine2-(2,4,5-Trimethoxyphenyl)-5,6,7,8-tetramethoxy-4H-1-benzopyran-4-oneC5:2Deltorphin CGratiogenin2-Hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate(6Z,9Z,12Z)-Octadecatrienoic acidValtratumLeu-Gly-Pro3alpha,7alpha,12alpha,26-Tetrahydroxy-5beta-cholestaneDigitaloseTyramineNiddamycin5-Methyl-5,6,7,8-tetrahydromethanopterin2,3,4,5-TetrahydrodipicolinateMetyraponeTrinexapac-ethyl3alpha,7alpha,12alpha-Trihydroxy-5beta-cholestan-26-al12-Hydroxydodecanoic acid(9Z)-Hexadecenoic acidOxoprolineValerolactoneHydroxybutanoic acidDeoxyhexoseNeuraminic acid5'-HydroxystreptomycinProclavaminic acidFormononetin3-PhosphonooxypyruvateEdrophoniumGlimepiride2,3-Dinor-8-iso prostaglandin F1alphaGoitrin5-(Heptadec-12-enyl)resorcinolHexanoic acidDecylubiquinol4-KetomyxolAcetyl-maltoseUndecylprodigiosinGlucovanillinC32:01,4-Bis(2-ethylhexyl) sulfosuccinateEuphorninDiethylcarbamazineLuteolinHymenoxonMycinamicin IVCitronellyl formate4-Sulfobenzoatetrans-4-HydroxycyclohexanecarboxylateAlanineApigenin 7-O-[beta-D-apiosyl-(1->2)-beta-D-glucoside]2-Oxosuccinamate4-Imidazolone-5-propanoateGlycogenN-Acetylputrescine3beta-HydroxysteroidTryptophanMethicillin sodiumXanthineD-MycinoseGlycyrrhetinate6-Oxabicyclo[3.1.0]hexane-2-undecanoic acid methyl esterAsparagine5beta-Pregnane-3alpha,17alpha,20alpha-triolIstamycin A1Tricrozarin A4-Sulfobenzyl alcoholAcetylcysteine1D-1-Guanidino-1-deoxy-3-dehydro-scyllo-inositolRimsulfuron7beta-Hydroxytaxusin2'-Hydroxybiphenyl-2-sulfinateDecanoic acid (FA10:0)4,8-DihydroxyquinolineAcanthicifoline7-DeoxyloganetinDaphninBremazocine(Z)-4-(2-Hydroxy-5-sulfonatophenyl)-2-oxo-3-butenoateC10:3Carbonic acid3A-Deolivosylpremithramycin BC10:13-Propylmalate7-Methylthioheptyl glucosinolateAcetylagmatineMenadione(9Z)-(13S)-12,13-Epoxyoctadeca-9,11-dienoic acidD-PrephenyllactatePuerarin xylosideHydroxyglutarateN1-Methyl-2-pyridone-5-carboxamideGeranylhydroquinone2-Pyrone-4,6-dicarboxylate4-MethylthiobutanaldoximeDoxercalciferolSolasodinePhyllanthin4-NitrocatecholDiosgenincis-4,5-Dihydroxy-4,5-dihydropyreneDaphnoretinC21:33beta,5alpha,6beta-CholestanetriolSedoheptuloseEthion(9Z)-(7S,8S)-Dihydroxyoctadecenoic acidNarbomycinMethyl-HisIntegerressine5-Methyl-2'-deoxycytidineFormyl-CoAAcetyl-GluMycalamide B12-Keto-leukotriene B4CorniculatusinUrocortisolDihydrothymineEleutheroside APeonidin-3-(p-coumaroyl)-rutinoside-5-glucosideUrocanateTestolate6-Acetyl-D-glucoseBurseran5alpha-Ergosta-7,22-diene-3beta,5-diolValine; BetaineDi(2-ethylhexyl) adipateN-Acetyl-D-glucosaminyl-N-acetylmuramoyl-L-Ala-D-glutamyl-6-carboxy-L-lysyl-D-alaninealpha-Oxo-benzeneacetic acid13,16-Docosadienoic acidUDP-acetyl-hexosamineZiprasidoneDethiobiotinMarinobufageninDodecanoic acid (FA12:0)MaltohexaoseEthyl 3-oxohexanoateRutinC3:0 (Propionic acid)Microhelenin CXylitolN-PhosphotaurocyamineDIDS5-Hydroxy-L-tryptophanPhosphonoformyl-CMPSinapatePipecolate(-)-Glyceollin IDeoxyloganic acid tetraacetatePfaffoside ADiphenylcarbazideBufadienolide3-O-Ethyl-L-ascorbic acidOctanoic acidOphiopogonin DConiferyl alcoholHydroxyadipateSalicin 6-phosphatePentoseEthyl isovalerate4-GuanidinobutanoateC12:4C12:1ThalidomideMethamidophos7,3'-Dihydroxy-4'-methoxy-8-methylflavanO-Succinyl-L-homoserine2S-Hydroxytetradecanoic acidDihydromyricetindTDP-L-mycarose5-Nitrofurfural5-O-Methylembelin3-(2-Carboxyethenyl)-cis,cis-muconateBiotin2,2-Dimethyl-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-7-ol acetateBz-Arg-OEtDecaline(R)-(Homo)3-citrateHexamethylphosphoramideLathodoratinTrilobinePhenol1-Ethyl-2-benzimidazolinonePheophorbide aN-(p-Hydroxyphenethyl)actinidine(Iso)Citrate5,6,7-TrimethoxycoumarinMethylphenidate16-Oxo-palmitate5-exo-Hydroxy-1,2-campholideTributyrinalpha-Tocopherol5-Acetamidopentanoate3-beta-D-Galactosyl-sn-glycerolGlycine3-(3,4-Dihydroxyphenyl)lactatePhenylacetyl-CoADinotefuranSclareolPfaffic acidL-Olivosyl-oleandolideCefixime3'-DeoxydihydrostreptomycinD-Glyceric acid17beta-Hydroxy-4,17-dimethyl-4-azaandrost-5-en-3-one3'-Deoxydihydrostreptomycin 6,3''-bis-phosphateTrihomomethioninePeganine7-Hydroxymethyl-12-methylbenz[a]anthracene sulfateDemeton-S-methyl2'-N-AcetylparomamineN'-NitrosoanatabineCathasterone17beta-(Acetylthio)estra-1,3,5(10)-trien-3-ol acetateBowdichioneGinkgolide AFurfural diethyl acetalPyrroline carboxylic acidSodium salicylateSativanine BButopyronoxylMethylselenopyruvateBetulinic acidEPTCFortimicin KL1Integerrenine5-Hydroxythiophene-2-carbonyl-CoAN-NitrosoguvacolineButoctamide hydrogen succinateProsolanapyrone II3-OxoglycyrrhetinateDMSOGuanosineSinapyl alcohol5-Hydroxyindoleacetylglycine(E)-4-(Trimethylammonio)but-2-enoateTrimethoprim(Iso)LeucineQuillaic acidPentose alcohol-P7-MercaptoheptanoylthreonineEsprocarbADPDisaccharide2-Methoxy-5Z-hexadecenoic acidProrocentrolideC14:1C14:0Kaempferol 3-sophorotriosideFormetanateNorethindrone enanthateMescalineAcetyl-HisCoproporphyrin IApioleP-homoserineEriojaposide ALoganinEriojaposide BMethylthioglycolic acidAmifostinedTDP-beta-L-rhodinose3-Isopropylcatechol5-O-(Indol-3-ylacetyl-myo-inositol) D-galactosideTribenuron methylNicotinurateCycloheptanecarboxylic acidSulfamethazineIproniazidUridineSoyasapogenol B 3-O-D-glucuronideIsopropylmaleateDimethyl-Arg2,3,6-TrihydroxypyridinePentadecanalPindolol1,2-Bis-O-sinapoyl-beta-D-glucosideVicianoseJuvenile hormone IIN-FeruloylglycineCymarinProlineSchizonepetoside EBenzoyl phosphatePR-toxingamma-L-Glutamyl-D-alanineNummularine F6-HydroxyindolelactateKinobscurinonealpha-1,5-L-ArabinotrioseAminobutanoic acid (ABA)Belladonninealpha-Amanitin2,4-Bis(acetamido)-2,4,6-trideoxy-beta-L-altropyranoseStanolone benzoateTomatidine1-Palmitoylglycerol 3-phosphateNDEAArachidonateN-(2,3-Dihydroxybenzoyl)-L-serineUreidoglycineMacrocin(-)-MenthoneGlucoiberinMontanolRyanodine8-Azaadenosine2-Hydroxymethylclavam(9Z,12Z)-(8R)-Hydroxyoctadeca-9,12-dienoic acid4-Nitroquinoline N-oxideDiphenoxylate3-HydroxyanthranilatePradimicin CUDP-hexoseZiziphinC16:0(10S)-Juvenile hormone III acid diolCondurangogenin CL-Cladinose2-Hexaprenyl-6-methoxy-1,4-benzoquinoneN-(Acetyloxy)benzenamine6-MethoxymelleinScytalone3-Ethoxyandrosta-3,5-dien-17beta-ol propanoateStipitatateTarennosideC16:3Triethylene glycol diglycidyl etherC16:2(3S,2'S)-4-Ketomyxol 2'-alpha-L-fucosideRindoside(S)-beta-MethylindolepyruvateMethylcytosineSanggenon EDiisononyl phthalateAcetone cyanohydrinAnhydrorhodovibrinAsimicinSuberic acid3-Hydroxy-vitamin KLuteolin 7-O-(6''-malonylglucoside)HexahomomethionineL-Ascorbic acid-2-glucosideSulfathalidineCeftriaxoneDelphinidin 3-O-3'',6''-O-dimalonylglucoside3,4-DihydroxyphthalateAcetylornithineDarlingine2-Hydroxy-2,4-pentadienoic acidCompactin diol lactoneLoganateOcthilinoneProsulfocarb3-Dimethylallyl-4-hydroxymandelic acidAspartatecis-(Homo)3-aconitatePyridoxineCorreolideCefazolinOxethazaineQuercitrinDiaminopimelateHydroxy-GluNONOatebeta-TocotrienolParomomycinLeurosineDihydrobiopterinBis(2-ethylhexyl)phthalateAcetoneDictamnineIngenol 3,20-dibenzoatePregnenolone acetateCapsaicinVersiconalBilirubinWestiellamide1-(beta-D-Ribofuranosyl)-1,4-dihydronicotinamideHippurateHydropreneZizybeoside Isn-Glycero-3-phospho-1-inositolBruceine DAcetyl-Asp8-(3,3-Dimethylallyl)spatheliachromene7-Methyl-2-hydroxy-6-oxoocta-2,4-dienoatePhenacetin2-Chloro-5-methyl-cis-dienelactoneFructoselysine 6-phosphateCyclopentanoneIndole-3-acetyl-myo-inositol2-Deoxy-D-ribose 1-phosphateC18:5HesperetinPterin9-cis-10'-Apo-beta-carotenalC18:2ProgeldanamycinPD 123319Thio-THIPMonomethyl sulfateRetinoate5-(2'-Carboxyethyl)-4,6-dihydroxypicolinate6-Amino-6-deoxyfutalosineCytidineAphidicolinIrinotecan carboxylateCaffeic acid 3-glucosideGlucoerucinCrotanecine22-Oxo-docosanoateHomostachydrine1-Hydroxy-6-methoxypyrenePhosphoserineGlaucolide BItaconateSudan IN-(4-Coumaroyl)-L-homoserine lactoneMethopreneCI Acid Orange 3Kaempferol 3-O-[6-(4-coumaroyl)-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside]Stearoylglycerone phosphate10-Hydroxydecanoic acidPyruvate oximeN-Acetyl-ala-ala-ala-methylesterStealthin C3-Hydroxy-2-(4-morpholinylmethyl)estra-1,3,5(10)-trien-17-one9-Methylthiononanaldoxime(R)-2-HydroxystearatePhenolphthalin1'-Acetoxychavicol acetateNaphthalic anhydride4-Amino-4-deoxychorismate(+)-ProsopinineAmylopectinPulcherriminic acidOleoylethanolamideCapsorubinPropylhydroxypentanoic acidErythromycin BErythromycin CL-(+)-AnaferineQuinateNeomethymycinToluene-cis-dihydrodiol4-Methylene-L-glutamate2-Imino-3-(7-chloroindol-3-yl)propanoateGypsogenin 3-O-rhamnosylglucuronideN-Ethylmaleimide3-Methyl-cis,cis-hexadienedioate2,5-DihydroxypyridineNaringenin3-(4-Hydroxyphenyl)pyruvateGlucoerysolinAllantoinEphedrineCarpacin2,3-Dihydroxy-p-cumateSyringintrans-2-[(Dimethylamino)methylimino]-5-[2-(5-nitro-2- furyl)-vinyl]-1,3,4-oxadiazoleGabaculineAndrostane-3,17-diol dipropionateEburicoic acidPhosphoenolpyruvate1-Guanidino-1-deoxy-scyllo-inositolHomoaconitateCholesteryl palmitategamma-Glutamyl-beta-aminopropiononitrile17beta-Hydroxy-6beta-methyl-5alpha-androstan-3-one propionateLanceotoxin AS-Methyl-3-phospho-1-thio-D-glycerateSuccinateFenitrothionGly-LeuCastanospermineMethionineMuramic acidChlorogenate4-Amino-2,6-dinitrotoluenePorphobilinogen2-OxosuberateSodium methallylsulfonateMethyl acetyl ricinoleateOtonecineAsebogeninCortisol 21-acetate3,9-DihydroxypterocarpanDopaquinoneTuliposide BAcetyl-Glu-semialdehyde4-PyridoxateCefpiramide sodiumPhosphoenol-4-deoxy-3-tetrulosonate4-O-beta-D-Glucosyl-4-hydroxycinnamateGlepidotin BClavulanic acidCarmine6-Ethylchenodeoxycholic acid12-OPDADelsolineFeruloylputrescineCinncassiol Bomega-Cyclohexylundecanoic aciddTDP-3-amino-2,3,6-trideoxy-C-methyl-D-erythro-hexopyranos-4-uloseCuauhtemoneUrateTetrahydroharmineSodium ortho-phenylphenate4-HydroxybenzoateShikimateProstaglandin F1alpha1,8-Diazacyclotetradecane-2,9-dione3-[(3aS,4S,7aS)-7a-Methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoateGlycidyl stearateSerotoninDeoxyuridineN-(3-Oxododecanoyl)homoserine lactoneFumarate8-HydroxyalanylclavamMolinate5alpha-Cholestan-5alpha,6beta-diolHeterodendrinAcetyl-MetButynalATPDihydroeuparinFonofosNicotianamineHexoseAgrimopholUDP-4-dehydro-6-deoxy-D-glucoseBerbamineHomoserine lactoneRehmaionoside C4-AcetamidobutanoatePretetramidAtratonCannabidiolic acidRehmaionoside AAcetylaminoadipateCortolMuscarinePhytosphingosine(R)-(Homo)2-citrateSpergualin3-HydroxykynurenineMilitarinone CIminoacetateEuxanthoneBarringtogenol CAbscisateBuddledin A2-Succinylbenzoate3-tert-Butyl-5-methylcatechol2-Hydroxytetracosanoic acidHarpagosidePhosphoglycerateTyrosine7-O-AcetylsalutaridinolAcetatic acid1,2,6-HexanetriolDihydroxy-methylthiopentanoic acid(Z)-But-1-ene-1,2,4-tricarboxylate1,3-Dicaffeoylquinic acid2'-NorberbamunineHomoarginine4,6-Diamino-5-formamidopyrimidineNebularineShikimate 3-phosphateKarounidiol5-Hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylateCoumarin(3S,5S)-Carbapenam-3-carboxylic acidDiethyl phenyl phosphatedTDP-4-oxo-2,3,6-trideoxy-D-glucoseVersicolorin B1,2-O-DiacetylzephyranthineCholine-P3-Oxotetradecanoyl-CoA3,4-DihydroxymandelateLinamarinO-PhosphoviomycinMegalomicin C1OxoadipateBetaxolol1-(5'-Phosphoribosyl)-5-amino-4-imidazolecarboxamideViguiesteninPhenacemide(R)-2-Hydroxybutane-1,2,4-tricarboxylateLevallorphan2-ArachidonoylglycerolPristimerinPyrogallic acidRishitinAurantio-obtusin beta-D-glucosideSulcatoneErgosineRhizocticin Bdi-trans,poly-cis-UndecaprenolLenacil3-Aza-A-homocholest-4a-en-4-oneC6:23alpha,7alpha,12alpha-Trihydroxy-5beta-cholestanoateResveratrolAtractyloside BMethyl palmitateOxaloglutarateCassiaside B2PhosphoramidonAsperlicin4-Heptyloxybenzoic acid2,7-Anhydro-alpha-N-acetylneuraminic acidLythramineAcetamideDeoxyvasicinoneN-Pyruvoyl-5-methoxy-3-hydroxyanthranilateCyclic di-3',5'-adenylateMC-207,1102-trans,6-trans-FarnesalEthambutolAilanthoneLactupicrinHomocysteineLumichromecis-2,3-Dihydroxy-2,3-dihydro-p-cumateGuanidinoproclavaminic acidRofecoxibAMPAPrimaquineZinnimidine8-Methylthiooctyl glucosinolateTolfenpyrad13(S)-HPOTSaccharocin2-MaleylacetateHistidinolD-Cathinone(S)-ATPATussilaginePimelateL-MimosineCamoensine2-BenzothiazolesulfonamideN-Glycosyl-L-asparagineCucurbitacin HNonanoic acid3-O-MethylquercetinNaringenin 7-O-beta-D-glucosideSebacic acidEndoxifenDevapamilMevalonateDihydrouracilMeconic acidSulfo-CysPhylloquinone8-Anilino-1-naphthalene sulfonic acidCucurbitacin STestosterone decanoate6beta-Hydroxy-17beta-estradiolPhytolaccoside BLupeol acetateNarasin BVomitoxinPhytyl phosphate5-(2-Hydroxyethyl)-4-methylthiazolePhthalateZoxazolamine(+)-NorephedrineYangambinbeta-D-4-Deoxy-delta4-GlcA-(1->4)-beta-D-Glc-(1->4)-alpha-L-Rha-(1->3)-beta-D-GlcDodemorphSalidrosideSamandaroneZeaxanthinAcrylamideD-GlucarateAntheraxanthinBRACO-19Apigeninsn-Glycero-3-phosphocholineKhellol glucosideEcdysone(+)-CamphorDeoxytubulosinePinolidoxinGDP-D-mannuronatePropanthelineErgosterolAsclepinOxaluric acidBiochanin A12-trans-Hydroxy juvenile hormone IIIDodecanedioic acidTyrindoxyl sulfate2alpha-Methyl-17beta-[(tetrahydro-2H-pyran-2-yl)oxy]-5alpha-androstan-3-oneDinitrosopentamethylenetetramine3-O-alpha-Mycarosylerythronolide BGDP-D-glycero-alpha-D-manno-heptoseMalvidin-3-(p-coumaroyl)-rutinoside-5-glucosideADP-riboseThevetin B4-HeptyloxyphenolRhodamine 1233-(3,4-Dihydroxyphenyl)pyruvateC20:113-Hydroxydocosanoic acid6-Mercaptopurine ribonucleoside triphosphatePhosphodimethylethanolaminePhenyl acetateLactateGoniodomin AThymidineCucumopine6-Keto-prostaglandin F1alphaKaempferol 3-O-beta-D-xylosideVitexin(R)-2-Methylimino-1-phenylpropan-1-olFlavonol 3-O-D-xylosylglucosidePantetheineKetovalineN-Acetyl-L-citrullineFurmecycloxOleandomycinCarlinosideCarnosifloside IIIBis-D-fructose 2',1:2,1'-dianhydride(24R,24(1)R)-Fucosterol epoxide3-Oxododecanoic acidFormimino-Glu17beta-Hydroxyestr-4-en-3-one cyclopentanepropionateGardenosideQuercetin 3-(2G-xylosylrutinoside)Lankacidin C5-(4-Acetoxybut-1-ynyl)-2,2'-bithiopheneOdoratolOphiobolin AQuercetin 3-O-[beta-D-xylosyl-(1->2)-beta-D-glucoside]Oxolinic acidSuccinylprolineAllyl-Cys3beta-Hydroxy-5alpha-cholest-7-ene-4alpha-carboxylateIndole-3-acetaldehydeL-Serine-phosphoethanolamine2-(6'-Methylthio)hexylmalic acidEthylketocyclazocineMercaptopyruvic acidN-NitrosomethylvinylamineOmethoateAcremoauxin ACarboxinLauroyl peroxide2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate1,2-DihydrosantoninFenthionTetrahydroxypteridine6-Acetamido-3-oxohexanoateStrigolactone ABC-ringsCajanolRiccionidin AFagaridine(S)-ACPAN-Acetyl-L-2-amino-6-oxopimelate10-OxodecanoateTHTCUDP-6-sulfoquinovoseLacto-N-tetraoseLG 100268HomogentisateMycothiolAzelaic acidC20817(Ac)2-L-Lys-D-Ala-D-Alacis-2-Carboxycyclohexyl-acetic acidN,N-Diethyl-2-[4-[6-methoxy-2-(4-methoxyphenyl)-1H-inden-3-yl]phenoxy]ethanamine hydrochlorideDihydroorotateAnatoxin a(s)Avermectin B2bDihydrozeatin riboside monophosphate2,5-Furandicarboxylate3-Sulfocatechol(9Z,11E)-(13S)-13-Hydroperoxyoctadeca-9,11-dienoic acidAcetoxoloneNicotinate5-HydroxyindoleacetateCallystatin AOxydeprofos2alpha-(Hydroxymethyl)-5alpha-androstane-3beta,17beta-diolThreonateDeoxycytidineCarbamoyl phosphateC22:5C22:42-Methyl-3-hydroxy-5-formylpyridine-4-carboxylateHistidylleucineC22:0falseGlass vs Clay: dynamic responses of the root exudate, root and shoot tissue metabolome upon SynCom colonisationPlant roots host defined microbial communities that differ from those in the surrounding soil, and these communities shift dynamically in response to plant development and environmental changes. Whilst it is widely accepted that root exudates play a key role in the assembly and dynamics of root-associated microbial communities, the underlying mechanisms are not well understood. This is partly due to a lack of controlled experimental systems that monitor both microbiome- and exudate-dynamics simultaneously. Focusing on bacteria, we compared two microcosm systems commonly used in either root microbiome (clay particle-based) or root exudate studies (glass bead-based) for their suitability to simultaneously monitor both aspects. After inoculation with a synthetic community (SynCom), we evaluated these systems for plant performance, bacterial growth, and time-resolved community and exudate profiling. Both systems were characterised by higher community evenness and Pseudomonas abundances in proximity to plant roots. While clay particles impeded exudate recovery, the glass bead set-up allowed to uncover dynamic exudate shifts during bacterial community establishment. With this system, a transient increase of glucosinolates upon root colonisation with initially dominant Pseudomonas species was detected. Overall, the comparison showed that only the glass bead-based semi-hydroponic system was suitable for the simultaneous study of root microbiome and exudate dynamics. The raw data of this study correspond to root exudates, root and shoot tissue extracts collected in a 2-week time series following metabolome responses to SynCom inoculation of plants grown in glass-microcosms.2026-09-162026-05-19MTBLS14526C01471; C05986C00207; C00479; C02001; C11506; C11507; C15508C06244; C06735; C11489C00033; C00266C01353C06145; C14275C06146; C17721; C19377; C20701C01659C00511; C00546; C19246; C19297; C19440C15809C00048C00163; C00424; C00937; C00969; C05235; C05999; C10920; C17530C00037; C01837; C06808; C19445C00160C00928; C11143C02648; C06144; C17601; C17602C00557; C07330C02659C00741; C01770; C01771; C02497; C16267; C16835; C19201; C19309; C19443C00022; C00222; C12069; C20363C00246; C00466; C00810; C00849; C01769; C02632; C14440C19282C00041; C00099; C00133; C00213; C01401; C01537; C02116; C11488C00209; C18586C00184; C00186; C00256; C00577; C01013; C01432; C02154; C02426C00116C02390; C06384; C20301C00146; C01782; C15584C11145C07090; C14279C07272C20441; C20578C07273; C11103C19524C02240; C08279; C12518; C14527; C15499; C19238C08305; C19285C01234; C02926; C07509; C08267; C11106; C17234; C19456; C19777C00109; C00164; C00232; C00349; C06002C00803; C08262; C18319C14422C02193; C11822; C20249C00334; C01026; C01205; C02261; C02356; C02721; C03284; C03665; C05145; C11109; C11735; C19514C00168; C00383; C01146C00989; C01089; C01188; C01991; C03197; C05984; C06001; C14601C03401; C06393C00065; C00716; C00740C03173C00258C20608C00556; C01403; C01467; C01468; C01542; C03485C02389; C05060C01987; C02372; C02720; C05058C00090; C00530; C01751; C01785; C11115; C15571C02560; C05130C01059; C02932; C03056; C03927; C05942C00380C06383C02704C06455C01546C00106; C18474C01066; C04091; C04221; C06105; C08063; C08502C03564; C03912; C04322; C07275C00596; C07091C00429; C02512; C02565; C02777; C08311C01147; C01880; C03228C19284; C19781C01596C00148; C00763; C16435; C18170C00122; C01384; C02150C00141; C02233; C03273; C06255C16622; C19283; C19484C01585; C02948; C03739; C12293; C12304; C12313C00990C00441; C03214; C03508; C20250C00183; C00431; C00719; C01799; C01826; C03571; C06417; C07457; C15987; C16436C11119C00042; C02170; C10900C14512C00188; C00263; C00820; C02115; C03678; C05519; C11108; C12317C00957C01796; C02022; C02045; C06463C00601; C02083; C05627; C06758; C07113; C07209; C07214; C20782C00097; C00736; C00793C09815C05823; C19296C00180; C00539; C00633; C03067; C06202; C15474C00253; C06813; C07446; C10164C00245C03557; C03782C02441; C02854; C14602; C14604; C16671; C18351C02376C01108; C02183; C02752; C02814; C11101; C11918; C20718C00178; C02835C04592; C12101C07287C03458; C19721C00906; C05715; C05717C01877; C01879; C02237; C04281; C04282C00408; C03969; C05936; C07500; C13696C02722C00433; C00490; C01732; C02132; C02214; C02226; C02800C00233; C00671; C00902; C01012; C02122; C02129; C03241; C03465; C03467; C03500; C06008; C06102; C12314; C14530C12290; C12296; C17714C02714; C19279C02362; C05840; C13058C16817C00430; C01015; C01073; C01110; C01157; C01165; C03341; C03440; C03651; C03741; C05147; C19706C00123; C00407; C01570; C01933; C02378; C02486; C06418; C08263; C16434; C16439; C16647C00036; C03548; C03981; C20341C00802C11074C00152; C01043; C01905; C02037; C02642; C16438; C19258; C19301C00077; C00515; C01602; C03943; C11320C00049; C00402; C16433; C19911C02091; C06382C17241C20313C00149; C00497; C00711; C03064C20360C00155; C05330C01620C00548; C01454; C03765; C05613; C06224; C07085; C07086; C07189; C07211; C07215; C10700; C10761; C14087; C15583; C20645C07587C00108; C00568; C01004; C14394; C19486; C19597C00483; C01183; C02735; C03473; C16570; C18396; C19216; C19376C00156; C00587; C00805; C05585; C10832; C16700; C18316C00785; C02126; C19345C14743; C16323C12110C06727; C16656; C18011; C19311C00169C18667C07505; C08287; C10128; C16821C00860C02595C14281C06231C12104; C12297; C16648; C16653; C16654; C19318; C19757C17357C04114; C10172C00922; C01757; C02295; C02930; C19821; C19822; C19826C04404; C16590C16837C06423; C07185C00940; C05572C04294C02796; C06324; C06338; C06343; C06415; C08493; C19434C02946; C03239; C03656; C04076; C05825C01035C00026; C03716; C05406; C06030; C06316C05851C00064; C00303; C00819; C05100; C16673C00025; C00217; C00302; C00979; C03618; C03790; C05574; C05938; C05941; C12269C17245C00652; C00684; C00815; C01087; C01114; C02266; C02612; C02614; C02630; C02753; C02994; C03196; C03826; C05405; C06029; C06031; C06032C00418; C00522; C02104; C03348; C04104; C06007; C06471C00073; C00855; C01733; C07418C20314C08264C08301; C17236C14872C02137; C03057; C10812C00121; C00181; C00216; C00259; C00309; C00310; C00312; C00476; C00508; C01394; C01488; C01508; C01510; C02096; C02204; C02205; C02479; C02604; C05052; C06115; C08346; C08353; C08354; C11476; C16639; C20569C00242C02709; C03005; C03522; C04350; C04353; C06804; C07301; C16571; C17755; C17756; C18623; C20634C02343; C02442; C03711; C07911; C08300; C10158; C11776; C16719C00385; C07599C00642; C00755; C01983; C01984; C02181; C02519; C02639; C02657; C03663; C04043; C05593; C05852; C10675; C10804; C12064; C12305; C13635; C13636; C14088; C14103; C14108; C17900C05842; C05843; C16398; C18320C00379; C00474; C00532; C01569; C01904C01014C13773C00808; C00809; C00964; C01123; C01499; C02213; C02452; C02759; C04435; C07271; C09847; C09859; C09885; C09893; C09906; C11387; C11395; C11398; C11401; C11408; C11409; C11412; C11415; C11938; C11941; C11942; C11951; C17623; C18018; C18369; C19081; C20260; C20425C00632; C01270; C02518; C06669; C07555; C11343; C12107; C12115; C16235; C19270C06502C03758; C04227; C16666C00196; C00230; C00628; C16748C06317; C10787C00843; C01411; C01500; C01765; C01766; C02344; C02462; C02484; C03039; C03985; C09844; C09848; C09871; C09902; C11384; C11388; C11389; C11390; C11393; C11396; C11397; C11399; C11400; C11410; C11411; C11413; C11416; C11952; C16772; C16909; C17073; C17125; C17384; C17517; C17518; C17611; C18017; C20261C02235; C15523C16283C17367; C19830; C19831C03059; C06056C00135; C00768; C01162; C06419C00988C20450C15997C04112; C04171; C04558; C04559; C04634; C06210; C06321; C06760; C18311; C20710C03680; C03817; C05568; C19481C02445; C08471; C08501C07411; C17361; C17724; C20828C08283; C10155; C12535C02222; C02501; C03453; C03676; C06035C00337; C03703; C10600C13711C01551; C02348; C02350C02631; C04670; C04815; C05126C01109; C16432C02975; C16652; C16655C01601C00651; C03623C00637; C06345; C10663; C14789C03087; C08269; C10151; C10850C00322; C00846; C04287; C06891; C16159; C20781C03012; C07719; C07726; C14785; C16213C02656; C04687; C16657C03864; C03865; C16649; C16650; C16651; C17527; C20793C02728C05637; C05639; C06330; C06342; C11503; C16716; C19837C00956; C01046; C01077; C02207; C03210; C05016; C06234; C17581; C19694C00318; C15025C07600C09315; C20414C15606; C17211C00478; C01088; C01989; C02360; C02488; C02991; C03652; C03761; C03827; C03979; C06158; C06159; C06474; C06478; C06485; C11592; C16390; C17209; C18028C04314; C04514; C06205; C06358; C10472; C10475; C10490; C12088; C15419; C20269C14764C03066; C03079; C05947C11196C00715C16759; C20204C04351; C06746; C11487C00166; C00811; C01772; C02763; C05838; C06738; C07114; C10945; C12621C01526C19485C00507; C00586; C00861; C01018; C01019; C01684; C01721; C02095; C02338; C02431; C02476; C02522; C02781; C07326; C08258; C08259; C08352; C16538; C20835; C20836C17243C06809; C12876C00079; C02057; C02265; C07527; C18090; C18747; C19565; C19578; C20487; C20488C19077C01575; C02765; C06199C01606; C03590; C06337; C14096; C14100C00502; C00545; C00878; C01685; C05411; C05412C03037; C09777; C09791; C09792; C09841; C09887; C09896; C11924; C11940; C14351; C14515; C17622; C17690; C17824; C18343; C18352; C20320C06976C17962C00250; C03493; C03986; C05831; C06051; C12323; C14170; C14171; C20821C01869; C04548; C05587; C07146; C07441; C07453; C07832; C08273; C19582C00074; C02798; C16849C13841C00975; C03459C00366C00544; C01161; C01839; C03198; C03235; C05343; C05577; C06207; C06672; C07087; C10331; C11527; C14109; C16215; C18346C13482C00314; C00547C01152; C03298C17730C19482C14280C07841C03092; C04433; C07276; C09790; C16462; C17621; C18020; C18027; C18066; C18202; C19082; C20326C03972; C12108; C17356C07569C10284; C11837C00093; C00623; C02979; C03189C02637; C02652; C03063; C05600; C07478; C07479; C18306; C18318C05377; C08446; C08774; C11427; C14091; C14101C20334C18472; C20602C16322C02131C01571; C02904; C12289; C12292C06325; C06414; C14040; C19474C01250; C12121; C17366C12346; C18487C02710; C10173C00417; C02341; C05422C00493; C04236; C16588; C18307; C18312C00439; C16674C08278; C14570; C16658; C19402C00437; C01047C00062; C00792; C02385C07473C01042; C01045; C05520C00954; C01386; C02298; C03230; C05634; C06331; C06344; C06525; C11504C10851C17249C03572; C03585; C04522; C07089; C16266C00072; C02670; C03217; C03289; C03387; C04053; C04349; C04471; C06033; C06430; C06440; C19806; C20364C02123; C02504; C03328; C04411C00780; C17203C11917; C18744C01624; C02666; C10667; C14784; C20327; C20646C07428C08235; C16287C10454; C10478; C14535; C14709; C17663; C17665; C17666; C17782C01586; C06332; C06339; C19569C07591; C09642; C10146; C14579; C17235; C18771; C18887C11777C13688C19250C01179; C01197; C01405; C01481; C05350; C12623; C13400C05380; C16623C00031; C00095; C00124; C00137; C00159; C00221; C00247; C00267; C00737; C00738; C00764; C00795; C00936; C00962; C00984; C01452; C01487; C01582; C01680; C01719; C01720; C01825; C01906; C01934; C02209; C02336; C02782; C05003; C06152; C06153; C06464; C06465; C06466; C06467; C06468; C08351; C08356; C10906; C15923; C19891C00590; C15475; C20343C03929C06050; C15770C01044C00082; C01536; C03290; C04368; C06420; C19579; C19712; C20807C18904C17941C01853; C06680; C12325; C14097C03672; C03964; C04044; C05582; C05583; C10447; C10680; C12622C12294; C14113C12283C00847; C04447; C04773; C11466; C12026C00788; C05589; C11768; C17925C13840C03232C03671; C08476C05580; C05616; C18724C02928C04535; C05594; C06582; C10765; C11456; C11588C00588C03843; C04718; C06066; C11405; C11419; C19079; C19084; C20324C12295; C13823; C13910; C19420C01005; C02532C10356; C10858; C12449; C20673C00197; C00597; C00631C13672C10659; C19116C14112C02217; C11404; C11406; C11417; C11420; C13945; C17620; C20714C17715C03682; C05548; C11842; C12988C20234C01092; C12029C18304; C18309C06678C04002; C04225; C11514; C11515; C16579C08261; C17878C02155; C02727; C03846; C12989C01924; C03884C02774; C18067C01037; C03793; C18885C00624; C03871; C12109C07437; C09348; C12345; C20635C19478C02256; C06326; C10124; C10132; C11843C11086C11081; C17251C06336; C06674C04110; C18078C03474C17220C17204C16589C19298C04546C00666; C00680C05635; C06725; C16596C02712C16850C17221C00158; C00311; C00451; C00679; C02780; C03600; C03921; C04575; C04617; C12649C01752; C01938; C03830; C04033; C06203; C06204; C10290; C16913; C18077; C18079; C20462C00296; C01295; C12113C10435; C10480; C10493; C12343; C14104; C16896; C17840; C20465C09907; C16151C16789C00779; C01494; C02379; C09974; C10470; C10477; C11233; C12204; C16982C03365; C03619; C03659; C03660; C03844; C05163; C06352; C06353; C08257C01297; C17964C14305; C16937; C17002C00822; C04274; C05604; C07588; C19631C03178; C03314C04045C00257; C00514; C00770; C00800; C00817; C00880; C03215; C15930C04604; C06661; C15771C13734; C17942C16394; C16395C02997; C12245; C19430C06580; C07351; C10664; C10684; C10726; C12205; C16778; C17455C00327C02264; C03752C04309C03223; C03233C01207; C05584; C10833C00198; C00204; C00273; C00691; C01040; C01115; C01216; C02669; C02692; C02779; C03107; C03269; C03383; C03926; C05394; C05410; C06892; C16737; C17577; C19679; C20251; C20680C04771; C06660C06579; C09853; C16644; C17454; C20789C09870; C12298; C16825; C16947C01102; C05702; C12147; C12215C06662; C11833C10660; C10697; C19269; C19473C06114C10411; C12448C07968C20209C00279; C03109; C03394; C03604C01036; C01061; C04642; C04783; C05592; C06318; C18314C06659; C17360C02679C05824C02236C06206C12248C20581C06658; C08271C18509C08277; C14566; C17803C01404; C03626C00331; C10127C12986C17253; C18755; C19097C05533C00078; C00525; C00806; C07839; C10968; C14916C02043; C05660; C09961; C10741C15480; C15481C05944C01251; C02225; C04593; C05662C09012; C09311; C14085; C14086; C14092; C20210C04000; C10476; C12458; C14567C19409C07499; C12590C01588; C02185C14876C00579C03519; C05620; C09933; C09986; C20126C02080C16201; C16202C09265; C20772C02147C02381; C05610; C09816C14236C16604C01458; C12231; C14262; C14334C10733; C13244C00818; C00879C05619C02076; C08236; C08355; C19612C02325; C07350; C10709C08491; C16317C06546; C07056; C07144; C07513; C11703C19098C17947C04366; C12624; C18338C10674; C11155C20790C11913C07827C08482; C08509; C16309; C16311C18206C11844C00672; C00673; C11437; C16637C18662C06819C13673C10254; C10269; C11834; C13687; C14341; C14349; C16015C09909; C17853; C17875C01909C02367C16640; C17076C06055C19268C01233C11711C08272C12027C18752C18830C11434C09243; C10148C08317C03914; C05644C15532C03017C18780C05341; C15699C17486C03738C08744; C08979; C09006C04020C04556C19125C01118; C03776C00864C13664C00643; C01017; C09985; C19716C03461; C09719; C09720; C14550; C14693; C16908; C16945; C16974; C17462; C17915; C17953; C17955; C19676; C19711; C19938; C20229; C20307; C20325C01111C05657C00140; C00645; C01132; C02711; C03136; C03878; C05021; C20328C04673C18668C02730; C09271; C10496; C12264; C14106; C14107; C17479; C17480C02655C10429; C10449; C14175; C19228; C20225C13708C09715; C16977; C16983C00482; C20653; C20654C18342C01760; C04166; C11512; C16318C06183; C06521; C11358C06984C07999C14186; C18768; C19106C19142C04277; C20514C08322C01948C05655; C06047; C06048C00826C10061C03872C03582; C09312; C09314; C09317; C09925; C14285; C14393; C15196; C19538C13733C16308C06424C11178C20291C00881C18655C10758C02678; C14523; C19143C03624C05539; C05932C20817C11732C02862; C04301C07196C10592C14514C14181C06742C16598; C16600C10426; C17917C18036C01223; C04130C11200; C18326C02253C17233C09350; C09376; C09399; C09409; C09677; C09686; C09712; C09721; C09739; C09743; C16942; C16949; C17090; C17489; C17491; C17492; C17504; C17609; C19678; C20309; C20404C03408; C03948C14169C14182C04399C19688C14742; C15042; C19433; C19479C09313; C09958C11255C20582C18249; 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