MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/m_MTBLS14531_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/m_MTBLS14531_LC-MS_negative_reversed-phase-c30_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/s_MTBLS14531.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/a_MTBLS14531_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531/a_MTBLS14531_LC-MS_negative_reversed-phase-c30.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14531Lipid identification was performed using a targeted MRM-based approach on the AB SCIEX QTRAP 6500+ LC-MS/MS platform.  Qualitative identification of lipids was strictly performed by matching the retention times (RT) and characteristic precursor-to-product ion transitions (Q1/Q3) against the self-built MWDB (Metware Database). The identification required:- Precise RT match (tolerance window of ± 0.1 min)- Matching Q1 (precursor ion) and Q3 (product ion) massesReference databases used for annotation included:- LIPID MAPS (http://www.lipidmaps.org/)- HMDB (Human Metabolome Database, https://hmdb.ca/)- KEGG (Kyoto Encyclopedia of Genes and Genomes, https://www.kegg.jp/)A total of 834 lipid species were annotated and quantified across major lipid categories, including glycerolipids, glycerophospholipids, glycolipids and sphingolipids.MetaboLightsPublicLiquid Chromatography MS - negative - reversed-phase-c30Liquid Chromatography MS - positive - reverse-phaseThe sample extracts were analyzed using an LC-ESI-MS/MS system (UPLC, ExionLC AD; MS, QTRAP 6500+ System).UPLC conditions:- Column: Thermo Accucore C30 (2.6 μm, 2.1 mm × 100 mm)- Solvent system: - A: acetonitrile/water (60/40, v/v, 0.1% formic acid, 10 mmol/L ammonium formate) - B: acetonitrile/isopropanol (10/90, v/v, 0.1% formic acid, 10 mmol/L ammonium formate)- Gradient program: - 0 min: A/B (80:20, v/v) - 2.0 min: 70:30 - 4 min: 40:60 - 9 min: 15:85 - 14 min: 10:90 - 15.5 min: 5:95 - 17.3 min: 5:95 - 17.3 min: 80:20 - 20 min: 80:20- Flow rate: 0.35 mL/min- Column temperature: 45 °C- Injection volume: 2 μLA targeted MRM-based lipidomic dataset of Tenebrio molitor larvae fed diets with different protein levels.Institute of Apicultural Research, Chinese Academy of Agricultural SciencesHansuo Liularvamass spectrometry assayLipids were extracted using an MTBE-based protocol. Frozen whole-body samples were thawed on ice and homogenized into a fine powder in liquid nitrogen using a Retsch MM400 ball mill at 25 Hz for 90 s, with a 60 s pause at the midpoint to prevent heating. An exact mass of 20 mg (±1 mg) of the homogenized powder was weighed. For extraction, 1 mL of extraction mixture containing MTBE/methanol (3:1, v/v) spiked with isotopic internal lipid standards was added to each sample. The mixture was vortexed for 15 min to ensure thorough homogenization and efficient lipid extraction. After vortexing, 200 μL of ultrapure water was added. The mixture was vortexed for 1 min and centrifuged at 12,000 rpm for 10 min at 4 °C. The upper organic layer (200 μL) was collected and evaporated to dryness using a vacuum concentrator. The dry extract was dissolved in 200 μL reconstitution solution (ACN/IPA = 1:1, v/v) for UPLC-MS/MS analysis. A pooled quality control (QC) sample was prepared by mixing equal aliquots of the reconstituted extracts from all 20 biological samples.Tenebrio molitorhttps://www.ebi.ac.uk/metabolights/MTBLS14531Raw mass spectrometry data were processed utilizing Analyst 1.6.3 software (AB Sciex, Framingham, MA, USA). Qualitative identification of lipids was strictly performed by matching the retention times (RT) and characteristic precursor-to-product ion transitions against the self-built MWDB (Metware Database), referencing universally accepted standard databases such as LIPID MAPS, HMDB, and KEGG.For quantitative analysis, peak area integration was performed on the extracted ion chromatograms (XICs). To ensure quantitative accuracy, internal standard normalization was applied. Identification required a precise RT match (tolerance window of ± 0.1 min) and matching Q1/Q3 masses. The absolute content of each lipid species (X, nmol/g) was calculated using the standard internal calibration ratio method formula: X=(0.001×R×c×F×V)/m, where R is the peak area ratio of target to internal standard, c is the internal standard concentration (μmol/L), F is the class-specific correction coefficient, V is the extraction volume (μL), and m is the sample mass (g). Protein levelliuhansuo@caas.cnTenebrio molitor larvae were obtained from a laboratory colony maintained on wheat bran at the State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China. Experimental design: Seven-week-old larvae were assigned to four dietary treatments for 12 weeks: standard wheat bran (Control), and custom-formulated feeds containing 15%, 20%, and 25% crude protein. The three formulated diets were designed to be isoenergetic. Each treatment included 5 independent biological replicates. Sample collection: At the end of the 12-week feeding trial, whole larvae were harvested from each dietary treatment. Approximately 30 fasted larvae were collected from one independent rearing tray and pooled as a single biological sample. Larvae were euthanized by immersion in liquid nitrogen within 10 s of removal and stored immediately at −80 °C until lipid extraction.Metabolomicstargeted analysisSamplelipid extractionSCIEX ExionLC AD UPLC systemintervention studyLiquid ChromatographyAB SCIEX QTRAP 6500+larvamass spectrometryTenebrio molitormzML formatlipidomicsdietary interventiontargeted analysisSamplelipid extractionSCIEX ExionLC AD UPLC systemintervention studyLiquid ChromatographyAB SCIEX QTRAP 6500+larvamass spectrometryTenebrio molitormzML formatlipidomicsdietary interventionLipid contents were detected by MetWare (http://www.metware.cn/) based on the AB Sciex QTRAP 6500 UPLC-MS/MS platform23. LIT and triple quadrupole (QQQ) scans were acquired on a QTRAP, QTRAP® 6500+ LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion modes and controlled by Analyst 1.6.3 software (Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature, 500 ℃; ion spray voltage (IS), 5500 V in positive mode and −4500 V in negative mode; Ion source gas 1 (GS1), gas 2 (GS2), curtain gas (CUR) were set at 45, 55, and 35 psi, respectively. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as multiple reaction monitoring (MRM) experiments with collision gas (nitrogen) set to 5 psi. Declustering potential (DP) and collision energy (CE) for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions was monitored for each period according to the metabolites eluted within this period.PE(O-18:1_18:0)LPI(20:3)PMeOH(16:0_18:1)PS(18:1_22:4)PS(18:0_18:1)PG(18:2_22:2)PE(20:1_18:2)FFA(18:0)FFA(30:1)PG(18:0_18:2)PG(21:1_18:2)PS(15:0_18:2)PS(21:0_18:3)PE(20:2_18:0)PI(16:0_18:3)PI(18:2_18:2)LNAPE(16:0/N-18:2)PMeOH(16:0_18:2)PS(18:2_16:0)PI(16:1_18:1)PE(O-18:1_18:1)FFA(18:1)PE(18:1_16:1)FFA(30:0)PE(O-18:0_18:2)PE(20:1_18:1)PG(16:0_17:2)PE(15:0_22:4)PE(17:0_18:2)PS(15:0_18:3)PS(16:0_17:0)LPS(16:0)CerP(d18:1/12:0)LNAPE(16:0/N-18:1)PA(18:1_22:6)LPG(17:0)PS(18:1_22:2)PG(18:2_16:0)PI(18:1_18:2)PE(18:1_22:2)9-HOTrEPE(14:0_20:2)FFA(16:4)LNAPE(16:1/N-16:0)PS(18:0_17:2)PE(O-18:0_18:1)PG(21:0_18:2)9,10-EpOMEFFA(17:1)PA(22:0_18:1)PI(18:0_14:1)CerP(d18:1/24:1)PE(17:0_18:1)PG(15:0_18:2)LNAPE(18:1/N-17:0)PI(16:0_18:1)PE(18:2_14:0)PG(18:2_16:1)PE(18:1_22:1)PE(18:2_19:1)taurolithocholicacid-3-sulfatePS(18:0_17:1)PG(18:2_21:2)PI(18:1_18:3)PA(18:0_22:5)PS(21:0_18:2)PE(16:0_16:0)PG(16:1_20:3)PI(16:0_18:0)PA(17:1_21:1)CerP(d18:1/18:0)PE(O-17:1_18:2)PE(O-18:0_20:3)PI(10:0_16:0)PA(16:0_18:2)PI(17:0_18:1)PE(20:0_18:1)Ursocholic acidPS(18:0_19:2)PI(15:0_20:1)LPA(18:2)FFA(16:2)LPA(18:1)PG(16:0_16:0)PA(16:1_22:5)PE(16:1_16:0)PS(16:0_20:5)PI(15:0_19:2)PE(18:2_16:0)FFA(28:0)FFA(22:2)CerP(d18:1/16:1)PE(14:0_18:1)PI(18:1_20:3)PE(20:0_18:2)LPI(16:0)PE(O-16:1_18:1)PG(16:0_22:1)FFA(17:0)PI(18:2_20:2)PG(16:0_16:1)LPA(18:0)PE(18:1_20:2)PI(15:0_19:1)PI(14:1_18:1)PI(19:0_17:2)PS(18:2_18:1)PA(20:1_18:2)PE(O-18:1_16:0)PI(18:1_20:2)PS(18:0_19:0)PS(18:0_18:3)PE(17:1_18:2)FFA(16:0)PG(18:2_20:2)PE(20:1_18:0)PG(16:0_16:2)PS(16:0_20:3)PE(O-16:0_18:1)PA(20:0_18:1)PE(18:1_20:3)LPG(16:0)PA(18:1_20:5)PE(20:0_18:0)PS(18:1_18:1)PE(18:2_20:2)PE(18:3_20:1)PI(17:0_18:2)PS(18:0_18:2)PA(16:0_22:5)PE(O-20:0_18:2)FFA(16:1)PI(15:0_20:2)PI(17:1_18:1)PS(15:0_18:1)PA(20:0_18:2)FFA(22:1)PG(18:1_20:3)PS(18:1_20:2)FFA(10:0)PS(21:0_16:1)PE(18:2_22:5)PE(22:0_18:1)PE(18:1_18:2)PE(18:0_14:0)PE(20:1_16:0)PA(16:0_20:2)PE(O-20:1_18:1)PS(16:0_20:1)PI(16:0_16:1)PG(14:0_18:2)PG(16:1_18:3)PS(15:0_16:0)LNAPE(16:0/N-16:0)PA(20:0_18:3)PI(18:2_16:0)PE(16:0_18:1)FFA(26:2)PS(18:1_20:3)PG(18:1_21:1)Taurocholic acidPI(19:0_15:1)PS(19:0_18:2)LNAPE(18:2/N-18:2)PE(O-18:0_16:0)PE(22:0_18:2)PE(18:1_18:3)PS(20:0_18:2)PI(16:0_16:0)PE(O-20:1_18:2)LPS(18:2)PE(O-18:3_18:2)PG(16:1_18:2)PE(22:6_18:2)PMeOH(16:0_16:0)PI(18:2_16:1)PS(18:2_18:2)PE(16:0_18:0)FFA(27:0)PG(18:0_22:0)PG(18:1_21:2)PA(16:0_20:0)lithocholicacid-3-sulfatePE(18:3_16:0)PA(22:1_18:2)PI(17:0_20:3)PG(18:2_18:2)PI(12:0_18:0)PS(17:0_18:1)PG(20:1_18:1)PI(18:2_20:3)LPI(17:0)PS(18:1_20:0)PE(16:1_18:0)LPS(18:1)FFA(26:0)PA(18:1_20:1)PA(18:2_20:2)PG(18:1_14:0)PG(18:0_22:1)PI(15:0_19:0)PE(16:0_18:3)PS(18:1_19:2)PG(18:1_19:1)PE(O-17:1_18:1)FFA(15:0)PG(18:2_18:3)PI(12:0_18:1)PG(18:0_16:3)PG(20:1_18:2)PA(18:1_20:2)PA(18:0_21:0)PI(15:0_18:2)LPG(15:0)PG(18:1_18:3)LPI(18:3)PI(18:0_18:3)PE(18:2_22:1)FFA(14:0)PE(18:2_18:3)PI(16:0_20:3)PS(18:2_20:3)Glycocholic acidPE(16:1_18:2)PG(17:0_22:1)PE(O-18:2_18:2)CerP(d18:1/18:3)PE(18:3_18:1)PE(O-22:1_18:1)LPG(18:0)12,13-EpOMEPS(18:1_22:1)PI(18:1_18:1)PI(18:0_18:2)PG(18:2_18:1)LPI(18:2)PS(18:2_20:2)PE(18:2_18:2)PS(17:1_18:1)PE(18:1_17:2)PS(16:0_18:1)PE(24:0_18:1)PE(20:2_18:3)PE(O-22:1_18:2)PI(19:1_18:2)PI(18:3_16:0)PA(20:0_21:0)PMeOH(18:0_18:2)FFA(20:1)PE(16:0_14:0)FFA(24:5)LPG(18:1)PG(16:0_18:3)PE(16:0_19:1)PG(18:1_18:1)PG(18:1_22:3)PA(18:2_22:5)PE(O-18:1_18:2)PE(18:1_18:0)LPI(18:1)PA(18:1_18:1)FFA(18:2)PS(23:0_18:1)PG(22:0_18:1)LPA(16:1)PG(22:1_18:2)PE(18:0_18:3)PS(18:0_16:1)PG(18:2_22:5)PA(18:2_18:2)PE(15:0_18:1)PS(18:3_20:3)PI(20:3_18:1)LPG(18:2)PS(17:2_18:2)PS(18:1_21:2)PI(18:1_17:2)PG(14:0_16:0)PE(18:1_18:1)PS(18:2_20:4)LNAPE(18:1/N-18:2)LPI(18:0)PA(18:1_18:2)FFA(18:3)PI(15:0_17:1)PI(13:1_18:1)PG(16:0_20:0)LPS(20:0)LPA(16:0)PE(18:0_18:2)9,10-DiHOMEPI(16:0_19:1)CerP(d18:1/14:0)PS(23:0_18:2)PE(O-18:2_18:1)falseA targeted MRM-based lipidomic dataset of Tenebrio molitor larvae fed diets with different protein levelsTenebrio molitor is increasingly used as a sustainable source of nutrients for food and feed production, yet their lipid metabolic responses to dietary protein remain poorly characterized at the molecular species level. Here, we report a targeted UPLC–MS/MS lipidomics dataset of Tenebrio molitor larvae fed four dietary treatments: a wheat bran control and three formulated diets containing 15%, 20% and 25% crude protein. The dataset includes 20 biological samples, with five independent replicates per treatment, each generated from pooled whole-body larvae. Using a scheduled multiple reaction monitoring method on a QTRAP 6500+ platform, 834 lipid species were annotated and quantified across major lipid categories, including glycerolipids, glycerophospholipids, glycolipids and sphingolipids. The data records comprise raw mzML files, processed concentration matrices, sample metadata, lipid annotation tables, MRM transition lists and quality-control metrics. Technical validation using pooled quality-control samples, coefficient-of-variation analysis, sample correlation, principal component analysis and hierarchical clustering supported the reproducibility of the dataset. This resource may facilitate studies of insect nutrition, dietary lipid remodeling, feed formulation and comparative lipidomics.2026-05-202026-05-20MTBLS14531