Metabolites were identified and quantified using an in-house mass spectral database. The raw data acquired after mass spectrometric analysis were processed using MassHunter software for qualitative and quantitative analysis.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Gas Chromatography MS - alternating - low-polarity"],"chromatography_protocol":["Chromatographic separation was achieved on a DB-5MS capillary column (30 m ? 0.25 mm ? 0.25 ?m). A 1-?L aliquot of each sample was introduced in split mode at a split ratio of 5:1. Helium served as the carrier gas and was maintained at a constant flow rate of 1.2 mL/min. The oven program started at 40?C with a 1-min hold, followed by heating to 100?C at 20?C/min and then to 300?C at 15?C/min. The final temperature was maintained at 300?C for 5 min.
"],"publication":["Profiling of Rhizosphere Metabolites."],"submitter_affiliation":["Antwerp university"],"submitter_name":["Miao Jiang"],"organism_part":["soil"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Fresh samples were weighed, immediately frozen in liquid nitrogen, and stored at ??0?C. Before extraction, samples were freeze-dried and ground into fine powder. Each powdered sample (0.5 g) was extracted with 1 mL methanol/isopropanol/water (3:3:2, v/v/v), vortexed for 3 min, and sonicated for 20 min. After centrifugation at 12,000 rpm for 3 min at 4?C, the supernatant was collected, mixed with 20 ?L internal standard solution (10 ?g/mL), dried under nitrogen, and lyophilized.
Mass spectral fingerprints of the samples were matched against reference spectra, and compounds were assigned based on matching scores. Retention index information was further used to refine metabolite annotation and reduce false-positive identification. Raw mass spectrometry data were processed with MassHunter quantitative analysis software. Selected quantifier ions were used for peak integration and correction to ensure reliable metabolite quantification.
"],"study_factor":["Treatment"],"submitter_email":["jiangmiao0203@163.com"],"sample_collection_protocol":["For rhizosphere soil collection, intact root systems were gently removed from the pots and shaken to eliminate loosely associated bulk soil. Four biological replicates were collected for each treatment. The soil firmly attached to the root surface, within an estimated 0?? mm distance, was defined as rhizosphere soil. This soil fraction was carefully dislodged with a sterile soft brush, placed in sterile tubes, and passed through a sterile 2-mm sieve after visible plant debris and stones had been removed.
"],"omics_type":["Metabolomics"],"study_design":["pooled quality control sample","Metabolomics","untargeted analysis","Agilent 5977B MSD","rhizosphere environment","nitrogen exposure","soil","Agilent 8890 GC","rhizosphere","untargeted metabolite profiling","experimental sample"],"curator_keywords":["pooled quality control sample","Metabolomics","untargeted analysis","Agilent 5977B MSD","rhizosphere environment","nitrogen exposure","soil","untargeted metabolite profiling","rhizosphere","Agilent 8890 GC","experimental sample"],"mass_spectrometry_protocol":["For mass spectrometric detection, electron ionization was applied at 70 eV. The temperatures of the transfer line, ion source, and quadrupole were set at 280?C, 230?C, and 150?C, respectively. Helium was used as the carrier gas throughout the analysis.
"],"additional_accession":[]},"is_claimable":false,"name":"Profiling of Rhizosphere Metabolites","description":"To assess whether maternal nitrogen history influences offspring rhizosphere metabolite profiles under contrasting soil nitrogen availability.","dates":{"publication":"2026-05-20","submission":"2026-05-20"},"accession":"MTBLS14537","cross_references":{}}