MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/m_MTBLS14548_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/m_MTBLS14548_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/a_MTBLS14548_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/s_MTBLS14548.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/a_MTBLS14548_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14548<p>Metabolite identification was based on retention time matching and mass spectral matching against a self-built reference database established by Biotree Biotech Co., Ltd.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>The chromatographic separation was performed using an ExionLC AD system (SCIEX). The column used was a Kinetex C18 column (2.1 millimeters × 100 millimeters, 2.6 micrometers). The mobile phase consisted of water with 0.01 percent formic acid (Phase A) and a mixture of 50 percent acetonitrile and isopropanol (Phase B). The flow rate was set at 0.3 milliliters per minute, the column temperature was maintained at 25 degrees Celsius, and the injection volume was 2 microliters.</p>Telomere-to-telomere genome of Stylosanthes guianensis uncovers symbiotic adaptation to phosphorus-deficient soils.Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences,Liu Pandaorootmass spectrometry assay<p>Freeze-dried samples were crushed with beads at 60 hertz for 30 seconds. Approximately 20 to 25 milligrams of tissue was weighed into a 2 milliliter tube, and 500 to 1000 microliters of extraction solvent (methanol:water = 3:1, volume per volume, containing internal standards) was added. Samples were vortexed for 30 seconds, homogenized at 40 hertz for 4 minutes, and sonicated on ice for 5 minutes. This homogenization-sonication cycle was repeated three times. Samples were extracted overnight at 4 degrees Celsius with shaking, then centrifuged at 12,000 revolutions per minute for 15 minutes at 4 degrees Celsius. Supernatants were filtered through a 0.22 micrometer membrane and transferred to autosampler vials.</p>Stylosanthes guianensishttps://www.ebi.ac.uk/metabolights/MTBLS14548Liu Pandao. Chinese Academy of Tropical Agricultural Sciences. liupd@catas.cn.<p>Raw data processing and quantitative analysis were performed using Biobud automated data processing software (developed by Biotree Biotech Co., Ltd.). The software performed peak extraction, integration, and normalization against internal standards.</p>BrTissueTimeliupd@catas.cn<p>Thirty-day-old Stylosanthes guianensis seedlings were subjected to two treatments: inoculation with Bradyrhizobium (Br) strain LZ3-2 and treatment without Br inoculation (Control). Following transplantation into acidic soil, root samples were harvested 60 days after transplanting, flash-frozen in liquid nitrogen, and stored at negative 80 degrees Celsius for subsequent flavonoid widely-targeted metabolomic analysis.</p>MetabolomicsMetabolomicsAB SCIEX QTRAP 6500+PlantSCIEX ExionLC ADStylosanthes guianensistargeted analysisroot nodulerootexperimental blankFlavonoidMetabolomicsPlantAB SCIEX QTRAP 6500+Stylosanthes guianensisSCIEX ExionLC ADtargeted analysisroot nodulerootexperimental blankFlavonoid<p>Mass spectrometric analysis was conducted using a SCIEX QTRAP 6500 plus triple quadrupole mass spectrometer equipped with an IonDrive Turbo V ESI source. The ionization mode was positive (POS) and negative (NEG). Typical ion source parameters were: IonSpray Voltage: positive 5500 volts / negative 4500 volts, Curtain Gas: 35 pounds per square inch, Temperature: 400 degrees Celsius, Ion Source Gas: 50 pounds per square inch. The scan mode was MRM (Multiple Reaction Monitoring).</p>falseTelomere-to-telomere genome of Stylosanthes guianensis uncovers symbiotic adaptation to phosphorus-deficient soilsThirty-day-old Stylosanthes guianensis seedlings were subjected to two treatments: inoculation with Bradyrhizobium (Br) strain LZ3-2 and treatment without Br inoculation (Control). Following transplantation into acidic soil, root samples were harvested 60 days after transplanting, flash-frozen in liquid nitrogen, and stored at negative 80 degrees Celsius for subsequent flavonoid widely-targeted metabolomic analysis.2026-05-212026-05-21MTBLS14548