Metabolites were annotated and quantified using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - reverse-phase"],"chromatography_protocol":["Chromatographic separation was performed using a Nexera X3 system (Shimadzu, Kyoto, Japan) equipped with a Discovery® HS F5 HPLC column (3 µm, 15 cm × 2.1 mm). The column type was reverse phase. Metabolites were measured using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.
"],"publication":["Voluntary Exercise Suppresses Prostate Cancer Growth by Remodeling Arginine Metabolism in Pten-Deficient Mice."],"submitter_affiliation":["The university of Osaka"],"submitter_name":["Toshiki Oka"],"organism_part":["Prostate Gland"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Cold methanol containing internal standards was added to tissue samples, followed by chloroform. The samples were homogenized using a bead-based homogenizer (TOMY, MS-100), sonicated for 5 min in an ice water bath, and centrifuged. Supernatants were collected, evaporated to dryness, and reconstituted in ultrapure water for LC-MS/MS analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14566"],"author":["Hase Hiroaki.","Toshiki Oka. toshiki.oka@gmail.com.","Hatano Koji. The University of Osaka. koj.hatan@gmail.com."],"data_transformation_protocol":["LC-MS/MS data were processed using LabSolutions software and the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu). The resulting metabolite abundance data were exported as processed data and organized by sample for downstream analysis.
"],"study_factor":["Exercise"],"submitter_email":["toshiki.oka@gmail.com"],"sample_collection_protocol":["Prostate tumor tissues were collected from Pb-Cre; Ptenfl/fl mice assigned to control or voluntary exercise groups. Tissues were rapidly excised, frozen, and stored until metabolite extraction.
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","prostate cancer","Mus musculus","Prostate Gland","Shimadzu LCMS-8050","targeted analysis","Metabolome","Shimadzu Nexera UHPLC system","experimental blank","Exercise Therapy"],"curator_keywords":["Metabolomics","prostate cancer","Mus musculus","Prostate Gland","Shimadzu LCMS-8050","targeted analysis","Metabolome","Shimadzu Nexera UHPLC system","experimental blank","Exercise Therapy"],"mass_spectrometry_protocol":["LC-MS/MS analysis was performed using an LCMS-8060NX triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan). Data were acquired with alternating polarity over a scan m/z range of 73–786 m/z. Metabolites were measured using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.
"],"additional_accession":[]},"is_claimable":false,"name":"Exercise-associated metabolomic changes in mouse prostate tumors","description":"This study investigated exercise-associated metabolic alterations in prostate tumor tissues from Pb-Cre; Pten (fl/fl) mice. LC-MS-based metabolomic profiling was performed to compare prostate tumor tissues from control and voluntary exercise groups.","dates":{"publication":"2026-05-23","submission":"2026-05-23"},"accession":"MTBLS14566","cross_references":{}}