MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14566/m_MTBLS14566_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14566/a_MTBLS14566_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14566/s_MTBLS14566.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14566/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14566<p>Metabolites were annotated and quantified using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p>Chromatographic separation was performed using a Nexera X3 system (Shimadzu, Kyoto, Japan) equipped with a Discovery® HS F5 HPLC column (3 µm, 15 cm × 2.1 mm). The column type was reverse phase. Metabolites were measured using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.</p>Voluntary Exercise Suppresses Prostate Cancer Growth by Remodeling Arginine Metabolism in Pten-Deficient Mice.The university of OsakaToshiki OkaProstate Glandmass spectrometry assay<p>Cold methanol containing internal standards was added to tissue samples, followed by chloroform. The samples were homogenized using a bead-based homogenizer (TOMY, MS-100), sonicated for 5 min in an ice water bath, and centrifuged. Supernatants were collected, evaporated to dryness, and reconstituted in ultrapure water for LC-MS/MS analysis.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14566Hase Hiroaki.Toshiki Oka. toshiki.oka@gmail.com.Hatano Koji. The University of Osaka. koj.hatan@gmail.com.<p>LC-MS/MS data were processed using LabSolutions software and the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu). The resulting metabolite abundance data were exported as processed data and organized by sample for downstream analysis.</p>Exercisetoshiki.oka@gmail.com<p>Prostate tumor tissues were collected from Pb-Cre; Ptenfl/fl mice assigned to control or voluntary exercise groups. Tissues were rapidly excised, frozen, and stored until metabolite extraction.</p>MetabolomicsMetabolomicsprostate cancerMus musculusProstate GlandShimadzu LCMS-8050targeted analysisMetabolomeShimadzu Nexera UHPLC systemexperimental blankExercise TherapyMetabolomicsprostate cancerMus musculusProstate GlandShimadzu LCMS-8050targeted analysisMetabolomeShimadzu Nexera UHPLC systemexperimental blankExercise Therapy<p>LC-MS/MS analysis was performed using an LCMS-8060NX triple quadrupole mass spectrometer (Shimadzu, Kyoto, Japan). Data were acquired with alternating polarity over a scan m/z range of 73–786 m/z. Metabolites were measured using the LC/MS/MS Method Package for Primary Metabolites, Version 3 (Shimadzu), according to the manufacturer’s protocol.</p>falseExercise-associated metabolomic changes in mouse prostate tumorsThis study investigated exercise-associated metabolic alterations in prostate tumor tissues from Pb-Cre; Pten (fl/fl) mice. LC-MS-based metabolomic profiling was performed to compare prostate tumor tissues from control and voluntary exercise groups.2026-05-232026-05-23MTBLS14566