MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/m_MTBLS14572_LC-MS_positive_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/m_MTBLS14572_LC-MS_negative_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/a_MTBLS14572_LC-MS_negative_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/a_MTBLS14572_LC-MS_positive_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572/s_MTBLS14572.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14572<p>Significantly Differential Metabolites (SDMs) were identified based on a strict combinatorial threshold: (1) Variable Importance in Projection (VIP) score &gt; 1; (2) Fold Change (FC) &gt; 2 or &lt; 0.5; and (3) False Discovery Rate (FDR)-adjusted P-value &lt; 0.05 (Benjamini-Hochberg correction,Wilcoxon rank-sum test).</p>MetaboLightsPublicLiquid Chromatography MS - positive - hilicLiquid Chromatography MS - negative - hilic<p>Chromatographic separation was performed on an Agilent 1290 UHPLC system equipped with a Waters Atlantis Premier BEH Z-HILIC column (1.7 μm, 2.1 × 150 mm). The mobile phase consisted of 10 mmol/L ammonium formate in water/acetonitrile (9:1, v/v) (Phase A) and 1:9 (v/v) (Phase B).</p>Nucleotide depletion signals early-stage soil stable carbon collapse in anthropogenically disturbed alpine ecosystems.Mengdi XieChengdu University of Technologynatural soildisturbed soilmass spectrometry assay<p>Soil metabolites were extracted and profiled using a targeted 600-MRM platform (Biotree, Shanghai). Briefly, 25 mg of soil was extracted using an acetonitrile-methanol-water system (2:2:1, v/v) spiked with isotopically labeled internal standards. Following homogenization with zirconia beads, low-temperature sonication, and centrifugation, the supernatant was analyzed. To monitor instrumental stability, quality control (QC) samples (pooled standard mixture) were injected every 10 samples.</p>composition of soilhttps://www.ebi.ac.uk/metabolights/MTBLS14572Mengdi Xie. Chengdu University of Technology. 291984698@qq.com.Xiaolu Tang. Chengdu University of Technology. 291984698@qq.com.<p>Raw data were processed using SCIEX Analyst Workstation (v1.7.3) and BIOTREE BioBud (v2.0.3) for peak integration and quantification. Preprocessing included filtration, missing value recoding, and internal standard normalization. Multivariate pattern recognition, including Principal Component Analysis (PCA) and Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA), was performed using SIMCA (v18.0.1).</p>Group291984698@qq.com<p>alpine soil under physical disturbance and the natural soil (the control).&nbsp;&nbsp;Samples were divided into two aliquots: one portion was air-dried for geochemical characterization (subset n = 78) and the remainder was sieved (2 mm) and flash-frozen at –80°C for multi-omics analysis.</p>Metabolomicsailpine grasslandMetabolomicstargeted analysisuntargeted analysis70-1200natural soilsoil organic carboncomposition of soilAgilent 1290 Infinity LCnatural toplayer soilAB SCIEX QTRAP 6500+disturbed toplayer soilLCMSdisturbed soilailpine grasslandMetabolomicstargeted analysisuntargeted analysis70-1200natural soilsoil organic carboncomposition of soilAgilent 1290 Infinity LCnatural toplayer soilAB SCIEX QTRAP 6500+disturbed toplayer soilLCMSdisturbed soil<p>Mass spectrometry was conducted on an AB Sciex QTrap 6500+ system with the following source parameters: IonSpray Voltage +5500V/-4500V, Curtain Gas 35 psi, Temperature 400°C, and Ion Source Gas 1/2 at 50 psi.</p>Benzoic acidPhenylpyruvic acidD-Glucuronic AcidL-PhenylalanineSucroseCorticosterone5-Methylcytosine11-Deoxycortisolbeta-Hydroxyisovaleric acidEctoineL-ThreonineAnserineDopamine 3-O-sulfateD-(+)-GlucosePantothenolKynurenic acidCaffeineL-LeucineTrigonellineIsoamylamineL-Citrulline4-Hydroxybenzoic acidCalcifediolUrocanic acidPropionylcarnitineL-ProlineUreaL-MethionineLevulinic-acid5-methyl-2'-deoxycytidineNicotinamideL-SorboseGlycerophosphocholine3-Methoxybenzoic acidTrehaloseCyclohexylamine5-MethylcytidineL-AsparagineN6-Acetyl-L-lysineDesoxycortonePicolinic acidMannitolN-AcetylserineL-TryptophanCreatinineSalicylic acid4-(Trimethylammonio)butanoateBenzoylformic acidXanthosineO-AcetylcarnitinePyridoxineTestosterone7-MethylguanineDeoxyguanosineTrimethylamineCyclamic acidL-Nicotine2-PhenylacetamideCholineN-Acetyl-L-alanineAcetylcholineL-Valine4-Aminobutyric acid4-Pyridoxic acidPantothenic acidHydroxyisocaproic acidGuanosineNicotinic acid3-Hydroxydecanoic acidThymidineDiphenylamineCreatineL-OrnithineOrotic acido-Methoxyphenyl sulfate17alpha-HydroxyprogesteroneBetaineL-Alanine2'-Deoxyuridine7-DehydrocholesterolCotinineTretinoinInositol2-Oxohexanoic acidH-LEU-VAL-OHAdrenosteroneErucamide2-Hydroxy-3-methylbutyric acidCoumarinDehydroepiandrosteroneNaringinUridineL-Aspartic acidHypoxanthineL-LysineQuinoline-2-carboxylic acid2'-DeoxyadenosineRetinalPhenoxyacetic acid2-PiperidoneL-Argininealpha-Linolenic acidIndoleD-GlucuronatePhenylethylamineMenatetrenoneAzelaic acidL-CarnitineDiethanolamineThiamineL-Glutamic acidEthanolamineDehydroandrosteroneAdenosineD-Tagatose1,1-Dimethyl-proliniumCholecalciferolThymineAdeninePhosphocreatineUric acidIndole-3-carboxylic acidL-Tyrosine1,5-Anhydro-D-GlucitolInosineL-SerineEstroneProgesteroneLactic acid3-Hydroxybutyric acidIsonicotinic acidfalseNucleotide depletion signals early-stage soil stable carbon collapse in anthropogenically disturbed alpine ecosystemsAnthropogenic disturbances are a primary cause of soil organic carbon (SOC) destabilization in alpine ecosystems. However, effective management is hindered by a """"response delay"""" of macro metrics used to characterize stable carbon pools (e.g., microbial necromass carbon and aggregate associated organic carbon), which respond slowly and therefore do not reflect the immediate impairment of the soil carbon sequestration. To address this, we conducted a multi-omics study on 200 soil samples along a 621-km transect. We observed a """"temporal lag"""": while physical disturbance caused rapid depletion of nucleotide metabolites, stable carbon fractions showed limited responsiveness, masking carbon depletion onset. Through metabolomics and 100-fold stratified subsampling, we identified depletion of specific nucleotides—notably thymidine and guanosine—as early-warning signatures (mean AUROC > 0.90). Metagenomic profiling revealed this depletion is driven by a disturbance-induced taxonomic shift triggering a synchronized suppression: the simultaneous inhibition of genetic capacity for de novo synthesis (mediated by pyrD) and salvage pathways (mediated by deoA). Furthermore, the concurrent lower abundance of korA indicates the disruption of the """"Glutamine Bridge,"""" effectively severing the metabolic link between nucleotide turnover and central carbon/energy metabolism. Our findings identify molecular """"early-warning biomarkers"""" that precede observable carbon loss, providing a sensitive tool for monitoring incipient soil degradation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