Metabolites were annotated by matching accurate mass-to-charge ratios, MS/MS fragmentation spectra, and chromatographic retention times against public databases (HMDB, METLIN, KEGG) and in-house spectral libraries.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Parameter Value[Chromatography Instrument]: Thermo Fisher Scientific UHPLC system (coupled to Q Exactive Orbitrap MS)Parameter Value[Autosampler model]: Thermo Fisher Scientific Vanquish or Dionex series autosampler (matching Q Exactive platform)Parameter Value[Column model]: ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm)Parameter Value[Guard column]: ACQUITY UPLC HSS T3 VanGuard guard column (2.1 mm × 5 mm, 1.8 μm)
"],"publication":["Energy-translation coupling limits yeast anaerobic growth."],"submitter_name":["Yongbo Wang"],"submitter_affiliation":["East China University of Science and Technology"],"organism_part":["Cell pellets"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Cell pellets were extracted with pre-chilled 80% (v/v) methanol and disrupted by ultrasonication in an ice-water bath. After incubation at −40 °C to precipitate proteins, the mixture was centrifuged at 12,000 × g for 10 min at 4 °C. The supernatant for LC-MS analysis was collected, lyophilized, reconstituted in 20% methanol, briefly sonicated, centrifuged again, and filtered through a 0.22 μm membrane prior to analysis.
"],"organism":["Saccharomyces cerevisiae"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14573"],"author":["Yongbo Wang. School of Biotechnology, East China University of Science and Technology. y12222081@mail.ecust.edu.cn."],"data_transformation_protocol":["LC-MS raw data were processed using Progenesis QI (Waters Corporation) for peak picking, alignment, retention time correction, and signal intensity normalization.
"],"study_factor":["Treatment"],"submitter_email":["y12222081@mail.ecust.edu.cn"],"sample_collection_protocol":["Saccharomyces cerevisiae cell pellets were collected for untargeted metabolomics analysis via LC-MS.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Orbitrap Fusion","Cell pellets","Metabolomics","Thermo Fisher Scientific","Energy","untargeted analysis","untargeted metabolites","experimental blank","Saccharomyces cerevisiae"],"curator_keywords":["Thermo Scientific Orbitrap Fusion","Cell pellets","Metabolomics","Thermo Fisher Scientific","Energy","untargeted analysis","untargeted metabolites","experimental blank","Saccharomyces cerevisiae"],"mass_spectrometry_protocol":["Parameter Value[Scan m/z range]: 70–1000 m/zParameter Value[Instrument]: Thermo Fisher Scientific Q Exactive Orbitrap mass spectrometerParameter Value[Ion source]: Electrospray ionization (ESI), operated in both positive and negative ion modesParameter Value[Mass analyzer]: Orbitrap high-resolution mass analyzer
"],"metabolite_name":["D-Hexose 6-Phosphate","Sorbitol","Zymosterol","D-Fructose","Monolaurin","Mg(18:0/0:0/0:0)","1-Tricosanol","Lophenol","Deoxycholic Acid","L-Histidine","4alpha-Methylzymosterol","Edetic Acid","Biotin","Citrulline","3-Hydroxyanthranilic Acid","17alpha-Hydroxypregnenolone","D-Glucuronic Acid 1-Phosphate","1-Kestose","Arachidic Acid","Phenylsuccinic Acid","Dehydroascorbic Acid","2,4-Dihydroxypentanedioic Acid","Glucosamine","Pantothenic Acid","1,5-Anhydrohexitol","Fructose 1-Phosphate","16beta,17beta-Estriol","2,3-Diphosphoglycerin","L-Tryptophan","Glutathione","Sorbitol-6-Phosphate","Glycyl-Glycine","Palatinitol","N-Acetyl-D-Glucosamine","Myo-Inositol","Galactinol","Pseudouridine","Cystathione","Adenosine Monophosphate","Cholesterol","Ginkgolide B","Myo-Inositol 1-Phosphate","Ginkgolide A","Inosinic Acid","N-Acetylmannosamine","Nicotianamine","Hydroxyphenyllactic Acid","3,4-Dimethoxyphenylethylamine","Guanosine","Morphine","Dodecanedioic Acid","Alizarin","Phytosphingosine","Chenodeoxycholic Acid","Salbutamol","Citric Acid","Fructose 6-Phosphate","Myristic Acid","Cyclic Amp","Clioquinol","D-Ribofuranose","Levan","1,25-Dihydroxyvitamin D3","Ht-2 Toxin","3-Deoxyhexonic Acid","Palmitic Acid(D3)","Uridine","Hypoxanthine","Galactofuranose-6-Phosphate","Pentadecylic Acid","D-(-)-Ribofuranose","2-Hydroxydecanedioic Acid","D-Glucose","Bilobol C15:1","Desmosterol","N-Alpha-Acetyl-L-Lysine","2-Hydroxyadenine","2-Hydroxy-N'-(1-(2-Thienyl)Ethylidene)Benzohydrazide","Monocyclohexyl Phthalate","1-(Sn-Glycero-3-Phospho)-1d-Myo-Inositol","11alpha-Hydroxyprogesterone","Octaethylene Glycol","Beta-Estradiol","Allocholic Acid","Mannitol 1-Phosphate","D-Mannonic Acid","Putreanine","5-Methyluridine","D-Gulose","Adenosine","Galactitol","Beta-Lactose","Adenine","Pterostilbene","Allose","Ergosta-7,22-Dien-3beta-Ol","L-3-Hydroxykynurenine","L-Tyrosine","Linoleic Acid","17-Octadecynoic Acid","Inosine","Norepinephrine","1d-Myo-Inositol 3-Phosphate","Melatonin","Sucrose","1-Methylindole-2-Carboxylic Acid","Ebericol","Glucose 6-Phosphate","Glucose","Mannobiose","Glycylproline","Galactose 1-Phosphate","D-(+)-Mannose","Cellobiose","Melibiose","Spermidine","Sphingosine","Methyl-Beta-Galactopyranoside","L-(-)-Sorbose","Saccharopine","Oxymorphone","D-Iditol","Carglumic Acid","Bisphenol A Monomethyl Ether","Galactonic Acid","Maltotriose","Arbutin","Deoxyadenosine","O-Succinyl-L-Homoserine","Obtusifoliol","L-Iditol","L-Cystathionine","Amoxicillin","Cysteinylglycine","Mannose 1-Phosphate","Arachidonic Acid","Hyenic Acid","Psicose","D-Galactose 6-Phosphate","Stearyl Alcohol","Leucrose","Isovalerylglucuronide","Cannabidiol","Glycyltyrosine","Beta-D-Glucopyranuronic Acid","9-Tetradecenoic Acid","5'-Methylthioadenosine","Kynurenine","D-Galactose","Ergosterol","Coniferin","Mg(16:0/0:0/0:0)","Xanthine","Deoxyguanosine","3-Beta-D-Galactosyl-Sn-Glycerol","Dihydrophytol","Norcodeine","Petroselinic Acid","Linoelaidic Acid","6-Phosphogluconic Acid","Ethyl Gallate","Alpha-D-Lactose","Oleic Acid","Gorgosterol","2-Methoxytyramine","Thymidine","Tranexamic Acid","D-Myoinositol 4-Phosphate","Gluconic Acid","D-Ribulose 5-Phosphate","Lactitol","Quinic Acid","Beta-D-Fructose 6-Phosphate","3-Hydroxy-Tetradecanoic Acid","Udp-N-Acetylglucosamine","(2-Hydroxy-3-Phosphonooxypropyl) Dihydrogen Phosphate","Fructose 1,6-Bisphosphate","Simvastatin","Squalene","Maltose","Chimyl Alcohol","L-Gluconic Acid","D-Fructose 2,6-Bisphosphate","L-Lysine","Sedoheptulose","Phosphoserine","Cis-Gondoic Acid","4-Hydroxy-3-Methylnaphthalene-1,2-Dione","Cytidine Monophosphate","5-Methoxyindoleacetate","Estrogen","Ethyl Beta-D-Galactofuranoside","2-Methylcitric Acid","L-Gulonic??Acid","2,5-Diaminopentanoic Acid","17-Hydroxy-6-Methylpregn-4-Ene-3,20-Dione","1-Hydroxy-3-Methoxy-6-Methylanthraquinone","Cis-Piceid","Nabilone","Palmitic Acid","Ursodeoxycholic Acid","Cadaverine","Beta-Mannosylglycerate","D-Tagatose","Stearic Acid","L-Altrose","Maltotriitol","Uridine 5'-Monophosphate","Beta-D-Glucose 6-Phosphate","Mg(0:0/16:0/0:0)","D-Mannose 6-Phosphate","Chrysin","Cis-9-Palmitoleic Acid","Estrone","3-Ethyl-3,4-Dihydro-2(1h)-Quinoxalinone"],"additional_accession":[]},"is_claimable":false,"name":"Energy-translation coupling limits yeast anaerobic growth","description":"This study employs yeast as a model system to thoroughly investigate the differences in regulatory mechanisms under various nutrient limitation conditions, utilizing a systems biology approach integrated with techniques such as multi-omics analysis.To obtain a comprehensive quantitative landscape of protein expression in (human lung adenocarcinoma), we performed TMT 6-plex–based comparative proteomic analysis. Tumour and adjacent non-tumour tissues (n = 6 pairs) were pulverised under liquid nitrogen and total proteins extracted by phenol–ammonium acetate/methanol precipitation. After reduction, alkylation and acetone precipitation, 50 µg proteins per sample were digested in-solution with trypsin (1:50, w/w, 37 °C, 16 h). Peptides were labelled with TMT 6-plex reagents, pooled and fractionated by high-pH reversed-phase chromatography on an Agilent 1100 HPLC (Zorbax Extend-C18, 15 fractions). LC-MS/MS was carried out on a Q Exactive HF-X (Thermo) in DDA mode. Quantitative analysis was performed with Proteome Discoverer 2.4 using a 1 % FDR threshold at PSM and protein levels. The dataset provides relative abundance information for > 6 000 protein groups across tumour and control tissues, serving as a resource for biomarker and pathway studies in lung adenocarcinoma.
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