MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/m_MTBLS14573_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/m_MTBLS14573_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/a_MTBLS14573_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/s_MTBLS14573.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/a_MTBLS14573_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14573<p>Metabolites were annotated by matching accurate mass-to-charge ratios, MS/MS fragmentation spectra, and chromatographic retention times against public databases (HMDB, METLIN, KEGG) and in-house spectral libraries.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Parameter Value[Chromatography Instrument]: Thermo Fisher Scientific UHPLC system (coupled to Q Exactive Orbitrap MS)Parameter Value[Autosampler model]: Thermo Fisher Scientific Vanquish or Dionex series autosampler (matching Q Exactive platform)Parameter Value[Column model]: ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm)Parameter Value[Guard column]: ACQUITY UPLC HSS T3 VanGuard guard column (2.1 mm × 5 mm, 1.8 μm)</p>Energy-translation coupling limits yeast anaerobic growth.Yongbo WangEast China University of Science and TechnologyCell pelletsmass spectrometry assay<p>Cell pellets were extracted with pre-chilled 80% (v/v) methanol and disrupted by ultrasonication in an ice-water bath. After incubation at −40 °C to precipitate proteins, the mixture was centrifuged at 12,000 × g for 10 min at 4 °C. The supernatant for LC-MS analysis was collected, lyophilized, reconstituted in 20% methanol, briefly sonicated, centrifuged again, and filtered through a 0.22 μm membrane prior to analysis.</p>Saccharomyces cerevisiaehttps://www.ebi.ac.uk/metabolights/MTBLS14573Yongbo Wang. School of Biotechnology, East China University of Science and Technology. y12222081@mail.ecust.edu.cn.<p>LC-MS raw data were processed using Progenesis QI (Waters Corporation) for peak picking, alignment, retention time correction, and signal intensity normalization.</p>Treatmenty12222081@mail.ecust.edu.cn<p>Saccharomyces cerevisiae cell pellets were collected for untargeted metabolomics analysis via LC-MS.</p>MetabolomicsThermo Scientific Orbitrap FusionCell pelletsMetabolomicsThermo Fisher ScientificEnergyuntargeted analysisuntargeted metabolitesexperimental blankSaccharomyces cerevisiaeThermo Scientific Orbitrap FusionCell pelletsMetabolomicsThermo Fisher ScientificEnergyuntargeted analysisuntargeted metabolitesexperimental blankSaccharomyces cerevisiae<p>Parameter Value[Scan m/z range]: 70–1000 m/zParameter Value[Instrument]: Thermo Fisher Scientific Q Exactive Orbitrap mass spectrometerParameter Value[Ion source]: Electrospray ionization (ESI), operated in both positive and negative ion modesParameter Value[Mass analyzer]: Orbitrap high-resolution mass analyzer</p>D-Hexose 6-PhosphateSorbitolZymosterolD-FructoseMonolaurinMg(18:0/0:0/0:0)1-TricosanolLophenolDeoxycholic AcidL-Histidine4alpha-MethylzymosterolEdetic AcidBiotinCitrulline3-Hydroxyanthranilic Acid17alpha-HydroxypregnenoloneD-Glucuronic Acid 1-Phosphate1-KestoseArachidic AcidPhenylsuccinic AcidDehydroascorbic Acid2,4-Dihydroxypentanedioic AcidGlucosaminePantothenic Acid1,5-AnhydrohexitolFructose 1-Phosphate16beta,17beta-Estriol2,3-DiphosphoglycerinL-TryptophanGlutathioneSorbitol-6-PhosphateGlycyl-GlycinePalatinitolN-Acetyl-D-GlucosamineMyo-InositolGalactinolPseudouridineCystathioneAdenosine MonophosphateCholesterolGinkgolide BMyo-Inositol 1-PhosphateGinkgolide AInosinic AcidN-AcetylmannosamineNicotianamineHydroxyphenyllactic Acid3,4-DimethoxyphenylethylamineGuanosineMorphineDodecanedioic AcidAlizarinPhytosphingosineChenodeoxycholic AcidSalbutamolCitric AcidFructose 6-PhosphateMyristic AcidCyclic AmpClioquinolD-RibofuranoseLevan1,25-Dihydroxyvitamin D3Ht-2 Toxin3-Deoxyhexonic AcidPalmitic Acid(D3)UridineHypoxanthineGalactofuranose-6-PhosphatePentadecylic AcidD-(-)-Ribofuranose2-Hydroxydecanedioic AcidD-GlucoseBilobol C15:1DesmosterolN-Alpha-Acetyl-L-Lysine2-Hydroxyadenine2-Hydroxy-N'-(1-(2-Thienyl)Ethylidene)BenzohydrazideMonocyclohexyl Phthalate1-(Sn-Glycero-3-Phospho)-1d-Myo-Inositol11alpha-HydroxyprogesteroneOctaethylene GlycolBeta-EstradiolAllocholic AcidMannitol 1-PhosphateD-Mannonic AcidPutreanine5-MethyluridineD-GuloseAdenosineGalactitolBeta-LactoseAdeninePterostilbeneAlloseErgosta-7,22-Dien-3beta-OlL-3-HydroxykynurenineL-TyrosineLinoleic Acid17-Octadecynoic AcidInosineNorepinephrine1d-Myo-Inositol 3-PhosphateMelatoninSucrose1-Methylindole-2-Carboxylic AcidEbericolGlucose 6-PhosphateGlucoseMannobioseGlycylprolineGalactose 1-PhosphateD-(+)-MannoseCellobioseMelibioseSpermidineSphingosineMethyl-Beta-GalactopyranosideL-(-)-SorboseSaccharopineOxymorphoneD-IditolCarglumic AcidBisphenol A Monomethyl EtherGalactonic AcidMaltotrioseArbutinDeoxyadenosineO-Succinyl-L-HomoserineObtusifoliolL-IditolL-CystathionineAmoxicillinCysteinylglycineMannose 1-PhosphateArachidonic AcidHyenic AcidPsicoseD-Galactose 6-PhosphateStearyl AlcoholLeucroseIsovalerylglucuronideCannabidiolGlycyltyrosineBeta-D-Glucopyranuronic Acid9-Tetradecenoic Acid5'-MethylthioadenosineKynurenineD-GalactoseErgosterolConiferinMg(16:0/0:0/0:0)XanthineDeoxyguanosine3-Beta-D-Galactosyl-Sn-GlycerolDihydrophytolNorcodeinePetroselinic AcidLinoelaidic Acid6-Phosphogluconic AcidEthyl GallateAlpha-D-LactoseOleic AcidGorgosterol2-MethoxytyramineThymidineTranexamic AcidD-Myoinositol 4-PhosphateGluconic AcidD-Ribulose 5-PhosphateLactitolQuinic AcidBeta-D-Fructose 6-Phosphate3-Hydroxy-Tetradecanoic AcidUdp-N-Acetylglucosamine(2-Hydroxy-3-Phosphonooxypropyl) Dihydrogen PhosphateFructose 1,6-BisphosphateSimvastatinSqualeneMaltoseChimyl AlcoholL-Gluconic AcidD-Fructose 2,6-BisphosphateL-LysineSedoheptulosePhosphoserineCis-Gondoic Acid4-Hydroxy-3-Methylnaphthalene-1,2-DioneCytidine Monophosphate5-MethoxyindoleacetateEstrogenEthyl Beta-D-Galactofuranoside2-Methylcitric AcidL-Gulonic??Acid2,5-Diaminopentanoic Acid17-Hydroxy-6-Methylpregn-4-Ene-3,20-Dione1-Hydroxy-3-Methoxy-6-MethylanthraquinoneCis-PiceidNabilonePalmitic AcidUrsodeoxycholic AcidCadaverineBeta-MannosylglycerateD-TagatoseStearic AcidL-AltroseMaltotriitolUridine 5'-MonophosphateBeta-D-Glucose 6-PhosphateMg(0:0/16:0/0:0)D-Mannose 6-PhosphateChrysinCis-9-Palmitoleic AcidEstrone3-Ethyl-3,4-Dihydro-2(1h)-QuinoxalinonefalseEnergy-translation coupling limits yeast anaerobic growth<p> This study employs yeast as a model system to thoroughly investigate the differences in regulatory mechanisms under various nutrient limitation conditions, utilizing a systems biology approach integrated with techniques such as multi-omics analysis.To obtain a comprehensive quantitative landscape of protein expression in (human lung adenocarcinoma), we performed TMT 6-plex–based comparative proteomic analysis. Tumour and adjacent non-tumour tissues (n = 6 pairs) were pulverised under liquid nitrogen and total proteins extracted by phenol–ammonium acetate/methanol precipitation. After reduction, alkylation and acetone precipitation, 50 µg proteins per sample were digested in-solution with trypsin (1:50, w/w, 37 °C, 16 h). Peptides were labelled with TMT 6-plex reagents, pooled and fractionated by high-pH reversed-phase chromatography on an Agilent 1100 HPLC (Zorbax Extend-C18, 15 fractions). LC-MS/MS was carried out on a Q Exactive HF-X (Thermo) in DDA mode. Quantitative analysis was performed with Proteome Discoverer 2.4 using a 1 % FDR threshold at PSM and protein levels. The dataset provides relative abundance information for &gt; 6 000 protein groups across tumour and control tissues, serving as a resource for biomarker and pathway studies in lung adenocarcinoma. </p>2026-05-242026-05-24MTBLS14573HMDB0013751HMDB0003156HMDB0000228HMDB0001536HMDB0000115HMDB0000161HMDB0000271HMDB0000008HMDB0002329HMDB0000700HMDB0000011HMDB0000407HMDB0244944HMDB0245045HMDB0031320HMDB0245013HMDB0061711HMDB0001906HMDB0000202HMDB0000883HMDB0252085HMDB0031654HMDB0000665HMDB0000060HMDB0001870HMDB0000149HMDB0256843HMDB0000695HMDB0000482HMDB0012275HMDB0000687HMDB0244061HMDB0000131HMDB0000766HMDB0001429HMDB0034778HMDB0000172HMDB0000162HMDB0000123HMDB0001488HMDB0000176HMDB0000957HMDB0001844HMDB0000254HMDB0000139HMDB0000300HMDB0002092HMDB0000134HMDB0000187HMDB0000847HMDB0000716HMDB0000532HMDB0032985HMDB0000167HMDB0253108HMDB0032037HMDB0000634HMDB0000262HMDB0034437HMDB0000661HMDB0245234HMDB0000466HMDB0006899HMDB0003011HMDB0245015HMDB0000349HMDB0255203HMDB0000719HMDB0000511HMDB0029932HMDB0000323HMDB0003911HMDB0001147HMDB0004437HMDB0011753HMDB0000744HMDB0002994HMDB0255916HMDB0000076HMDB0001406HMDB0000696HMDB0000191HMDB0032538HMDB0001169HMDB0000267HMDB0244182HMDB0000725HMDB0000991HMDB0000112HMDB0000943HMDB0254466HMDB0250791HMDB0000402HMDB0004284HMDB0059655HMDB0250341HMDB0000208HMDB0000779HMDB0251528HMDB0000355HMDB0000816HMDB0000214HMDB0251512HMDB0000148HMDB0028680HMDB0000159HMDB0000098HMDB0062642HMDB0011676HMDB0000638HMDB0242608HMDB0000751HMDB0000742HMDB0304340HMDB0041922HMDB0000283HMDB0003157HMDB0000168HMDB0000398HMDB0000223HMDB0001392HMDB0001851HMDB0000568HMDB0000640HMDB0242149HMDB0302754HMDB0001112HMDB0001901HMDB0002520HMDB0001414HMDB0252849HMDB0000232HMDB0000226HMDB0000072HMDB0000867HMDB0000126HMDB0001586HMDB0002064HMDB0000224HMDB0000362HMDB0000641HMDB0014457HMDB0001138HMDB0000784HMDB0000635HMDB0003070HMDB0000807HMDB0000094HMDB0000904HMDB0000157HMDB0011733HMDB0000290HMDB0000272HMDB0002000HMDB0001264HMDB0000806HMDB0002322HMDB0244228HMDB0001476HMDB0000143HMDB0000034HMDB0000755HMDB0000122HMDB0304632HMDB0015673HMDB0001151HMDB0000182HMDB0003418HMDB0011632HMDB0000247HMDB0000379HMDB0250327HMDB0000177HMDB0000158HMDB0000107HMDB0033836HMDB0000826HMDB0001514HMDB0242119HMDB0250761HMDB0000565HMDB0000625HMDB0000721HMDB0000210HMDB0041806HMDB0253886HMDB0250766HMDB0000095HMDB0003229HMDB0000292HMDB0001129HMDB0000220HMDB0015109HMDB0000078HMDB0000424HMDB0000215HMDB0001548HMDB0000623HMDB0254852HMDB0002091HMDB0000446HMDB0000618HMDB0061656HMDB0000403HMDB0002350HMDB0000216HMDB0000099HMDB0004096HMDB0000125HMDB0001047HMDB0250769HMDB0303077HMDB0006270HMDB0000207HMDB0000929HMDB0002080HMDB0001257HMDB0245396HMDB0000827HMDB0001389HMDB0000673HMDB0244776HMDB0304157HMDB0003971HMDB0000124HMDB0130987HMDB0003498HMDB0000767HMDB0001436HMDB0011631HMDB0001401HMDB0000645HMDB0001043HMDB0304312HMDB0246205HMDB0000273HMDB0302859HMDB0003976HMDB0001313HMDB0000213HMDB0001316HMDB0002231HMDB0000884HMDB0005831HMDB0001937HMDB0002212HMDB0248144HMDB0000296HMDB0000030HMDB0000279HMDB0000252HMDB0000145HMDB0001530HMDB0011533HMDB0000258HMDB0014440HMDB0060657HMDB0000195HMDB0011564HMDB0028853HMDB0004610HMDB0015323HMDB0302572HMDB0000151HMDB0000101HMDB0000050HMDB0001262HMDB0000055HMDB0000058HMDB0001058HMDB0014629HMDB0000163HMDB0040937HMDB0094680HMDB0036619HMDB0041627HMDB0000133HMDB0011131HMDB0000186HMDB0011729HMDB0000085HMDB0013682HMDB0001173HMDB0000256HMDB0003539HMDB0000045HMDB0000175HMDB0000288HMDB0011649HMDB0000347HMDB0015193HMDB0029943HMDB0006814HMDB0002361HMDB0000363HMDB0006271HMDB0005007HMDB0000067HMDB0000878HMDB0002719HMDB0001217HMDB11605HMDB0001242HMDB0000505HMDB0031422HMDB0302590HMDB0000626HMDB0001903HMDB0000518HMDB0000946