MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/m_MTBLS14589_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/m_MTBLS14589_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/s_MTBLS14589.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/a_MTBLS14589_LC-MS_negative_hilic_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/a_MTBLS14589_LC-MS_positive_hilic_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14589The R package and the BiotreeDB (V3.0, China) were applied in metabolite annotation. Candidate metabolites (VIP > 1 and adjusted P < 0.05) were regarded as potential biomarkers.MetaboLightsPublicLiquid Chromatography MS - positive - hydrophilic interactionLiquid Chromatography MS - negative - hydrophilic interaction - Thermo Orbitrap Exploris 120Chromatographic separation was carried out on a UHPLC system with a Waters Acquity UPLC BEH Amide column (1.7 µm, 2.1 × 50 mm). Mobile phases A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) used a gradient elution (0–10 min: 5–95% B) at 0.3 mL/min; column temperature was 4°C, and injection volume was 2 µL.Fermented brewers' grains modulate serum and pectoral muscle metabolome to regulate flavor quality formation in broilers.Liaocheng UniversityYuanfeng Limusclemass spectrometry assaySamples were extracted using 400 μL of extraction solution (MeOH:ACN, 1:1 (v/v). The process included homogenizing samples on ice for 5 min, centrifuging at 12,000 rpm for 10 min at 4°C, and collecting supernatants for analysis.Gallus gallushttps://www.ebi.ac.uk/metabolights/MTBLS14589Li Yuanfeng. Liaocheng University. Liaocheng University, Liaocheng, Shandong, China. 309271207@qq.com.The raw mass spectrometry data were converted to mzXML format using ProteoWizard software (ProteoWizard Software Foundation, USA) for data standardization, then processed with an in-house R program based on XCMS for peak alignment, retention time correction, and data normalization to ensure consistency for downstream analysis.Dietary treatment309271207@qq.comAfter euthanasia, the right pectoralis major muscle was rapidly dissected. Tissue blocks (0.5 cm × 0.5 cm × 0.5 cm) were collected from the middle part, avoiding fascia and adipose tissue. Samples were rinsed with pre-cooled sterile saline, dried with filter paper, snap-frozen in liquid nitrogen, and stored at −80 °C until metabolomic analysis.MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicspooled quality control sampleUntargeted metaboliteswet-fermented brewer's grainsuntargeted analysisThermo Scientific Orbitrap Exploris 120musclenutrition interventionGallus gallusexperimental sampleThermo Scientific Vanquish UHPLC SystemMetabolomicspooled quality control sampleUntargeted metaboliteswet-fermented brewer's grainsuntargeted analysisThermo Scientific Orbitrap Exploris 120musclenutrition interventionGallus gallusexperimental sampleThe Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS spectra on information-dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). In this mode, the acquisition software continuously evaluates the full scan MS spectrum. The ESI source conditions were set as following:sheath gas flow rate as 50 Arb, Aux gas flow rate as 15 Arb, capillary temperature 320 ℃, full MS resolution as 60000, MS/MS resolution as 15000, collision energy: SNCE 20/30/40, spray voltage as 3.8 kV (positive) or -3.4 kV (negative), respectively.Dimethyl (9E,18E)-1,10-bis(.beta.-D-glucopyranosyloxy)-6,15-dioxo-4a,5,6,8,13a,14,15,17-octahydro-1H,10H-dipyrano[3,4-d:3',4'-k][1,8]dioxacyclotetradecine-4,13-dicarboxylate(2-{[3-(hexadecanoyloxy)-2-[octadeca-9.12-dienoyloxy]propyl phosphono]oxy}ethyl)trimethylazaniumGlutathione disulfideLPG(18:1(9Z))PG(16:0/18:1)(2-aminoethoxy)[2-[icosa-5.8.11.14-tetraenoyloxy]-3-[octadec-11-enoyloxy]propoxy]phosphinic acid2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphoserineTaurohyodeoxycholic acidTauroursodeoxycholic acid1-Stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-sn-glycerol)Nis(monooleoylglycero)phosphate (S,R Isomer)(2-aminoethoxy)[2-[octadec-9-enoyloxy]-3-(octadecanoyloxy)propoxy]phosphinic acid(2-aminoethoxy)[2-[icosa-5.8.11-trienoyloxy]-3-(octadecanoyloxy)propoxy]phosphinic acid(2-aminoethoxy)[2-[docosa-4.7.10.13.16.19-hexaenoyloxy]-3-[hexadec-1-en-1-yloxy]propoxy]phosphinic acid[2-[docosa-4.7.10.13.16.19-hexaenoyloxy]-3-(octadecanoyloxy)propoxy]({[2.3.4.5.6-pentahydroxycyclohexyl]oxy})phosphinic acid1-Stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-myo-inositol)1,2-Distearoyl-sn-glycero-3-phospho-L-serineTaurocholic acid2-amino-3-({[3-(hexadecanoyloxy)-2-(octadecanoyloxy)propoxy](hydroxy)phosphoryl}oxy)propanoic acid[2-[docosa-7.10.13.16-tetraenoyloxy]-3-(octadecanoyloxy)propoxy]({[2.3.4.5.6-pentahydroxycyclohexyl]oxy})phosphinic acid2-Arachidonoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamineMaltotriose2-amino-3-({hydroxy[2-(icosanoyloxy)-3-(octadecanoyloxy)propoxy]phosphoryl}oxy)propanoic acid1-Stearoyl-2-arachidonoyl-sn-glycero-3-phosphoserineChebulinic acidTaurochenodeoxycholic acid2-amino-3-({hydroxy[2-(icosanoyloxy)-3-[octadec-11-enoyloxy]propoxy]phosphoryl}oxy)propanoic acidPS(18:0/18:2)Cholesteryl sulfateAsparasaponin INicotinamide adenine dinucleotide (NAD)2-amino-3-({hydroxy[2-[icosa-5.8.11-trienoyloxy]-3-(octadecanoyloxy)propoxy]phosphoryl}oxy)propanoic acidEthanox 310PE(18:0/18:2)Taurodeoxycholic acidLPC(13:0)Tauro-gamma-muricholic acid[3-(hexadecanoyloxy)-2-[icosa-5.8.11-trienoyloxy]propoxy]({[2.3.4.5.6-pentahydroxycyclohexyl]oxy})phosphinic acid1-Stearoyl-2-oleoyl-sn-glycero-3-phospho-(1'-sn-glycerol)PI(16:0/18:1)2-Linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamine20-((6-O-Hexopyranosylhexopyranosyl)oxy)-14-hydroxypregn-5-en-3-yl 2,6-dideoxy-4-O-(6-deoxy-3-O-methyl-4-O-((2E)-2-methylbut-2-enoyl)hexopyranosyl)-3-O-methylhexopyranosideADP-ribose2-Docosahexaenoyl-1-stearoyl-sn-glycero-3-phosphoserineCyclic adenosine diphosphate-ribose (cADPR)PC(16:0/20:4)1-palmitoyl-2-linoleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)(2-aminoethoxy)[2-[docosa-4.7.10.13.16.19-hexaenoyloxy]-3-[octadeca-1.9-dien-1-yloxy]propoxy]phosphinic acidTauro-beta-muricholic acid(2-aminoethoxy)[2-[docosa-4.7.10.13.16-pentaenoyloxy]-3-(octadecanoyloxy)propoxy]phosphinic acidfalseFermented brewers' grains modulate serum and pectoral muscle metabolome to regulate flavor quality formation in broilersThis study was designed to systematically assess the impact of wet-fermented brewer's grains (WFBG) on pectoral muscle metabolic profiles and key regulatory pathways in broiler chickens. In this 21-day experiment, a total of 281 DEMs were identified, with 29 upregulated and 252 downregulated DEMs (P < 0.05). These DEMs were mainly enriched in 61 metabolic pathways, involving amino acid anabolism, lipid metabolism, and flavor precursor transformation pathways. This study elucidated the metabolomic mechanism by which WFBG regulated broiler meat quality and filled the research gap regarding the effects of WFBG on flavor formation and muscle nutrition in broilers. The findings provided a theoretical basis and practical reference for the large-scale application of WFBG in broiler diet formulation. 2026-05-272026-05-27MTBLS14589