MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14642/m_MTBLS14642_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14642/a_MTBLS14642_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14642/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14642/s_MTBLS14642.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14642<p>Lipid annotation was performed in MS-DIAL based on accurate mass and MS/MS spectral matching. Only features with MS/MS available were retained for downstream analysis. The adducts considered for annotation were restricted to [M−H]− and [M+CH3COO]− in negative ionization mode, and [M+H]+, [M+Na]+, and [M+NH4]+ in positive ionization mode.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p>Lipid extracts were analyzed by UHPLC–MS/MS in both positive and negative ionization modes. Chromatographic separation was performed on an Accucore™ C30 column (150 × 2.1 mm, 2.6 μm; Thermo Fisher Scientific, Waltham, MA, USA) using a Vanquish™ UHPLC system (Thermo Fisher Scientific). Separation was conducted using a 20-min gradient at a flow rate of 0.35 mL/min and a column temperature of 40 °C. Mobile phase A consisted of acetonitrile/water (6:4, v/v) with 10 mM ammonium acetate and 0.1% formic acid, and mobile phase B consisted of acetonitrile/isopropanol (1:9, v/v) with 10 mM ammonium acetate and 0.1% formic acid.</p>Differential control of brown adipose tissue function by dietary and Scd-derived MUFAs reveals a non-redundant role of Scd2.Nanjing agricultural universityTINGXUAN GAOBrown Adipose Tissuemass spectrometry assay<p> Briefly, 0.75 mL methanol was added to each 100 mg tissue or 106 cells in a centrifugation tube with a Teflon-lined cap and vortexed thoroughly. Then, 2.5 mL MTBE was added and the mixture was incubated for 1 h at room temperature with shaking. Phase separation was induced by adding 0.625 mL MS-grade water. After 10 min incubation at room temperature, samples were centrifuged at 1,000 × g for 10 min. The upper (organic) phase was collected, and the lower phase was re-extracted with 1 mL MTBE/methanol/water (10:3:2.5, v/v/v). The combined organic phases were dried and reconstituted in 100 μL isopropanol prior to LC–MS/MS analysis. A pooled QC sample was prepared by combining equal aliquots from each biological sample and was processed in parallel with biological samples</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14642TINGXUAN GAO. Nanjing agricultural university. 2022208014@stu.njau.edu.cn.<p>Raw LC–MS/MS files were processed using MS-DIAL for peak detection, MS/MS deconvolution, alignment, and lipid annotation. Centroid mass tolerances were set to 0.01 Da for MS1 and 0.025 Da for MS2. Peak detection was performed with a minimum peak height of 1000 amplitude and a mass slice width of 0.1 Da. Smoothing was applied using a linear weighted moving average with a smoothing level of 3 scans, and the minimum peak width was set to 5 scans. MS/MS deconvolution was conducted using a sigma window value of 0.5, an MS/MS abundance cutoff of 10 amplitude, and an MS/MS relative abundance cutoff of 1%. Only features with MS/MS available were retained. The exported feature table was further curated by applying a fill rate cutoff >0.6 across all samples and reducing redundant entries derived from multiple adducts or closely overlapping aligned peaks. One representative feature was retained from each redundancy group based on annotation confidence and signal robustness.</p>TemperatureMUFA content2022208014@stu.njau.edu.cn<p>Brown adipose tissue was rapidly collected from mice after sacrifice. The interscapular brown adipose tissue depot was carefully dissected, cleaned of surrounding connective tissue and visible white adipose tissue, and immediately snap-frozen in liquid nitrogen. Frozen samples were stored at −80°C until lipid extraction and LC-MS/MS-based lipidomic analysis.</p>MetabolomicsMus musculusWaters ACQUITY UPLC H-Class Systembrown adipose tissueuntargeted analysisMUFALipidomicsexperimental blankcold exposureThermo Scientific Q Exactive HFMus musculusWaters ACQUITY UPLC H-Class Systembrown adipose tissueuntargeted analysisMUFALipidomicsexperimental blankcold exposureThermo Scientific Q Exactive HF<p>The UHPLC system was coupled with a Q Exactive™ HF/Q Exactive™ HFX mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Mass spectrometry was performed in both positive and negative ionization modes. The MS parameters were as follows: sheath gas 40 psi, auxiliary gas 10 L/min in positive mode and 7 L/min in negative mode, spray voltage ±3.5 kV, capillary temperature 320 °C, heater temperature 350 °C, S-lens RF 50, scan range m/z 144–1700, AGC target 3e6 for MS1 and 2e5 for MS2, maximum injection time 100 ms, isolation window 1 m/z, normalized collision energy 22/24/28 eV, and dynamic exclusion 6 s.</p>DG 36:4|DG 18:2_18:2PC 31:0PI 40:3|PI 20:0_20:3PE 40:10PE 38:4|PE 18:2_20:2CL 70:6|CL 16:1_18:2_18:1_18:2Cer 42:1;O2|Cer 18:1;O2/24:0PS 40:4|PS 18:0_22:4PE O-34:1|PE O-18:1_16:0CL 68:5|CL 16:1_18:1_16:1_18:2LPE 24:0DG 40:9TG 54:4|TG 18:1_18:1_18:2LPE 24:5PE 32:2|PE 16:1_16:1DG 40:4PC O-32:1LPE 24:6PC O-32:0DG 40:3FA 19:0PE P-36:5|PE P-16:0_20:5DG 40:2PC O-32:2DG 40:1DG 40:8FA 19:1DG 40:7DG 40:6PC O-32:6DG 40:5PE 38:5PE 38:4PE 38:3SM 40:3;O2CL 72:10|CL 18:2_18:3_18:2_18:3PE 38:8PE 38:7PE 38:6TG 47:0|TG 15:0_16:0_16:0LPC 17:2/0:0PC 30:2|PC 12:0_18:2PE O-38:8|PE O-18:3_20:5PC 44:12|PC 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lipidomic remodeling in mouse brown adipose tissue under a 2 × 3 experimental design involving environmental condition and dietary fatty acid composition. Mice were maintained at room temperature with ad libitum feeding or exposed to 4°C cold conditions with 70% calorie restriction. Under each condition, mice were fed diets differing in fatty acid composition: a low-fat LM diet, a high-MUFA HM diet, or a high-SFA HS diet. Brown adipose tissue samples were analyzed by LC-MS/MS-based lipidomics to characterize diet- and cold-associated changes in lipid species, lipid classes, and lipid metabolic profiles.2026-06-012026-05-31MTBLS14642CCCQTEPLTYUPRM-WZUSAPNINA-NCERRQJMSXAXGRO-NFYLBXPENA-NCNGSOZKJBWHOEG-VBOJLMESNA-NCDLDTSWOMZKEBV-UHFFFAOYNA-NCJKXNIAUCJAYKE-LJURNQLPNA-NCGPMXFFJDVINHX-NFYLBXPENA-NCPYYIMXJKFANHS-ZPHPHTNENA-NCITHEXJVPOWHKC-UHFFFAOYNA-NCAVQYQUIVKHMQF-JRQMLGJJNA-NCUVBMQAPZWKFCF-GBCPRSIONA-NCJKXGRSGRKUWTH-AWDDGWGJNA-NCXEIFEBACMREHD-WQEXLHBVNA-NCDIDDLXSXXDECX-HAGCXRLMNA-NCNKNQKFREZNPNM-BWAHOGKJNA-NCKGVBFQDYSPMSY-UHFFFAOYNA-NCZOSTDZGCCEZTJ-PEZBUJJGNA-NCBDUTJYWWDMHBN-ICFOKQHNNA-NCLPMAPXZURYSNH-ZARCXLEVNA-NCMMXUBGHSNQBNN-ZCXUNETKNA-NCQSXCZMDDSQLAG-SWVFECATNA-NCDPGTJRMESRMBM-MAHFBRAONA-NCAPRXGZRZWSDGE-UHFFFAOYNA-NCFERVOXBORHKHR-JLNKQSITSA-NCAPHXUZAFHNJAV-KUBAVDMBNA-NCWZGHHQNICLNJN-MEQMIZENNA-NCFQRNKTWTBEFHB-SZRPQGNZNA-NCAIZNNKLZCGMJJ-ZLGQNYCKNA-NCMHAYOZHFZADHZ-GYQRTUFANA-NCFYWMETWXXZSHR-XPKLYHLONA-NCXLZYQKWZLBZEB-YVAAUFMTNA-NCLQKBJHAUYGCOB-HSBBONCLNA-NCLVGZBAEAVIJRD-DONOITMCNA-NCOQJUHVGXCZWNN-ZLKDJVMPNA-NCVDYOYNJWLXMAW-LKVUMQLBNA-NLMWGIQYUPHCPHA-GGLIIMCGNA-N