Metabolite identification was performed using Agilent MassHunter workstation software and its built-in databases and spectral libraries.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Gas Chromatography MS - positive - medium-polarity"],"chromatography_protocol":["GC-MS analysis was performed using an Agilent 7890B gas chromatograph system coupled with a Agilent 5977B mass spectrometer. The system utilized a DB-FastFAME capillary column. A 1 μL aliquot of the analyte was injected in split mode (5:1). Helium was used as the carrier gas, the front inlet purge flow was 3 mL/min, and the gas flow rate through the column was 46 psi with constant pressure. The initial temperature was kept at 75 °C hold on 1 min; raised to 200 °C at a rate of 50°C/min, hold on 15 min; raised to 210 °C at a rate of 2 °C/min, hold on 1 min; raised to 230 °C at a rate of 10 °C/min, hold on 16.5 min.
"],"publication":["Cold exposure aggravates vein occlusion through non-shivering thermogenesis-induced thrombocytopoiesis."],"submitter_affiliation":["Fudan University"],"submitter_name":["Ruibo Chen"],"organism_part":["blood serum"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Take 50μL sample into the 2 mL EP tubes, extracted with 450 μL extracting solution (V Isopropanol:V n-Hexane=2:3, containing 0.2mg/L internal standard), vortex mixing for 30s. Ultrasound treated for 10 min (incubated in ice water). Centrifuge for 15 min at 12000 rpm, 4 °C. Transfer the supernatant into a fresh 1.5 mL EP tubes. Add 500μL of extract solution (VIsopropanol:Vn-Hexane=2:3, containing 0.2mg/L internal standard) to the remaining samples, vortex mixing for 30 s, repeat steps 2, 3. The supernatant from step 5 and step 3 were combined and vortexed for 10 s. Take 800 μL of the combined supernatant and nitrogen blow dry. Add 500 μL of methanol:trimethylsilyl diazomethane solution (1:2) , standing at room temperature for 30 min. Nitrogen blow dry. Add 160μL of n-Hexane and redissolve; Centrifuge for 1 min at 12000 rpm. Transfer the supernatant into a fresh vial for GC-MS analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14667"],"author":["Yunlong Yang. Fudan University. yunlongyang@fudan.edu.cn."],"data_transformation_protocol":["Raw data were acquired in Agilent GC-MS/MS format (.D) and processed using Agilent MassHunter workstation software.
"],"study_factor":["Temperature"],"submitter_email":["17301010009@fudan.edu.cn"],"sample_collection_protocol":["Mouse serum samples were freshly made and frozen in liquid nitrogen.
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","Mus musculus","targeted analysis","Agilent 7890B GC","Agilent 5977B MSD","blood serum","Serum","Venous thrombosis","targeted metabolite profiling","experimental sample"],"curator_keywords":["Metabolomics","Mus musculus","Agilent 7890B GC","targeted analysis","Agilent 5977B MSD","blood serum","Serum","Venous thrombosis","targeted metabolite profiling","experimental sample"],"mass_spectrometry_protocol":["The injection, transfer line, quad and ion source temperatures were 240 °C, 240 °C, 230 °C and 150 °C. The energy was -70 eV in electron impact mode. The mass spectrometry data were acquired in Scan/SIM mode with the m/z range of 33-400 after a solvent delay of 7 min.
"],"additional_accession":[]},"is_claimable":false,"name":"Cold exposure aggravates vein occlusion through non-shivering thermogenesis-induced thrombocytopoiesis","description":"Vein occlusion (VO), including deep venous thrombosis (DVT) and retinal vein occlusion (RVO), is a common cause of multiple diseases that severely compromise the quality of life of affected individuals. Epidemiological evidence indicates that VO prevalence increases in cold seasons, yet the underlying mechanism remains unknown. Here, we show that cold exposure markedly elevates peripheral platelet counts, thereby aggravating VO in mouse models. Cold-augmented thrombocytopoiesis depends on the activation of adipose thermogenesis and subsequent elevation of circulating free fatty acids (FFAs). Mechanistically, FFA-β-oxidation promotes acetyl-CoA production, which upregulates and stabilizes C/EBPα through shifting the balance between p300 acetyltransferase and SIRT1 deacetylase. Acetyl-C/EBPα transcriptionally upregulates GATA-1 and NF-E2 for megakaryocyte maturation and platelet production. Depletion of adipose triglyceride lipase PNPLA2, megakaryocyte-specific knockout of key β-oxidation enzyme CPT1α, or pharmacological inhibition of CPT1α and p300 abolishes cold-augmented thrombocytopoiesis and alleviates DVT and RVO in mouse models. In healthy volunteers, tolerable cold exposure activates adipose thermogenesis, increases circulating FFAs, and upregulates platelet counts. Moreover, a retrospective cohort study of 425 patients reveals elevated platelet counts and higher DVT incidence during cold seasons. Similarly, increased platelet counts are observed in 448 patients with RVO at the time of diagnosis in cold seasons. Together, our study provides novel mechanistic insights into the increased VO risk induced by cold exposure and proposes a new therapeutic paradigm for VO by targeting megakaryocyte metabolism.","dates":{"publication":"2026-07-08","submission":"2026-06-03"},"accession":"MTBLS14667","cross_references":{}}