MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/m_MTBLS14700_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/m_MTBLS14700_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/a_MTBLS14700_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/s_MTBLS14700.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/a_MTBLS14700_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB1_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC1_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC2_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC4_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB3_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC3_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB4_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC3_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC4_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB2_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB4_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC2_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB3_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/MC1_pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB2_neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700/FILES/RAW_FILES/OB1_pos.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14700<p>Metabolite annotation was performed by matching accurate mass and MS/MS spectra against public databases including HMDB, Metlin, and KEGG. The mass tolerance was set to 5 ppm for precursor ions and 0.02 Da for fragment ions. Identifications were confirmed by comparison with authentic standards where available.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed on a Thermo Fisher Vanquish UHPLC system equipped with a Waters Acquity HSS T3 column (1.8 μm, 2.1 × 100 mm). Mobile phase A was 0.1% formic acid in water, and mobile phase B was acetonitrile with 0.1% formic acid. The gradient elution program was: 0–1 min, 5% B; 1–9 min, 5–95% B; 9–12 min, 95% B; 12–12.1 min, 95–5% B; 12.1–15 min, 5% B. The flow rate was 0.3 mL/min, column temperature was 40°C, and injection volume was 2 μL.</p>High-glucose microenvironment facilitates cancer stemness of canine osteosarcoma via HBP/O-GlcNAc signaling cascade.Northeast Agricultural UniversityHonggang FanOsteosarcoma derived cell lineOsteoblast derived cell linemass spectrometry assay<p>Metabolites were extracted using ice-cold methanol-water solution (4:1, v/v). Briefly, cell pellets were homogenized in extraction solvent, vortexed, and sonicated in an ice bath. After centrifugation at 12,000 g for 15 min at 4°C, supernatants were collected and dried under vacuum. The dried extracts were reconstituted with methanol-water (1:1, v/v) before analysis. A pooled quality control (QC) sample was prepared by mixing equal volumes of all samples and injected at regular intervals throughout the run.</p>Canis lupus familiarishttps://www.ebi.ac.uk/metabolights/MTBLS14700Weiqian Wang. Northeast Agricultural University. weiqianwang784@gmail.com.Honggang Fan. Northeast Agricultural University. fanhonggang2002@163.com.<p>Raw LC-MS data were processed using Progenesis QI software (Waters) for peak picking, alignment, normalization, and feature detection. Retention time alignment was performed using the default settings. The peak intensities were normalized to the total ion count (TIC) of each sample.</p>Cell typefanhonggang2002@163.com<p>Canine osteosarcoma McKinley cells and normal canine osteoblast PEKCC2382 cells were cultured at Northeast Agricultural University. Cells were harvested in logarithmic growth phase, washed with ice-cold PBS, and immediately snap-frozen in liquid nitrogen, then stored at -80°C until metabolite extraction. Three biological replicates were prepared for each cell line.</p>MetabolomicsNortheast Agricultural UniversityMetabolomicsProteoWizard msconvertThermo Fisher Orbitrap Exploris 120 mass spectrometerOsteosarcoma derived cell lineMckinlycanine osteosarcomaCanine cell lineuntargeted analysisThermo Fisher Vanquish UHPLC systemOsteoblast derived cell lineexperimental sampleuntargeted metabolite profilingPEKCC2382LC-MSCanis lupus familiarisR programming languageXCMSOsteoblasttumor metabolismNortheast Agricultural UniversityMetabolomicsProteoWizard msconvertThermo Fisher Orbitrap Exploris 120 mass spectrometerOsteosarcoma derived cell lineMckinlycanine osteosarcomaCanine cell lineuntargeted analysisThermo Fisher Vanquish UHPLC systemOsteoblast derived cell lineexperimental sampleuntargeted metabolite profilingPEKCC2382LC-MSCanis lupus familiarisR programming languageOsteoblastXCMStumor metabolism<p>Mass spectrometry analysis was performed on a Thermo Fisher Orbitrap Exploris 120 mass spectrometer with an electrospray ionization (ESI) source. The instrument was operated in both positive and negative ionization modes. The spray voltage was set to 3.5 kV (positive) and 3.0 kV (negative). The capillary temperature was 320°C, and the auxiliary gas heater temperature was 350°C. Sheath gas flow rate was 40 arb, auxiliary gas flow rate was 10 arb, and sweep gas flow rate was 1 arb. Full scan MS spectra were acquired with a mass range of m/z 100–1000 at a resolution of 60,000.</p>ConiferaldehydeThymidine-5'-monophosphoric acidGondoic AcidCyclic cmpXanthosine-5'-monophosphatePiceatannol1-Hydroxypyrene-7,8-oxideL-AllothreonineInositol cyclic phosphate5,10-(Methanylylidene)tetrahydromethanopterin2-Hydroxy-7-hydroxymethylchromene-2-carboxylateKelnacGlycolaldehyde phosphate7-hydroxylysineFluridoneCastasteroneCytidine 5'-monophosphate-N-acetylneuraminic acidVittatineCassaidineN-Methylethanolaminium phosphateCyclooctatinMaltose 1-phosphateBrassicasterolecdysone palmitate2-(2-chlorophenyl)-5-(5-methyl-2-thienyl)-1,3,4-oxadiazoleProstaglandin E2PinostilbenedTDP-beta-L-4-epi-vancosamineSilybinIsosinomenine ACMP-Leg5Ac7AlaBoldioneGlutathioneGnidicin(r)-3-Amino-4-methylpentanoic acid3-VinylcatecholAlpha-D-Galactose-1-PhosphateNovamethymycinAdenosine-5'-diphosphateOleamide4-Amino-5-hydroxymethyl-2-methylpyrimidineN-Methyl-L-prolineditrans,tetracis-Heptaprenyl diphosphateColfosceril palmitate2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-oneEudesmin7,7',8,8'-TetrahydrolycopeneD-Pyroglutamic acid6-BenzylaminopurineSalicylaldehydeN2-SuccinoylarginineAspartic Acid3-Hydroxy-1-indanonecis-1,2-Dihydro-3-ethylcatechol7-carboxy-7-deazaguanineNIFURTHIAZOLE2-Amino-5-epi-valiolone5-chlorohydroxyquinonePhthalic acidAminoadipic acid2-Thioxo-4-thiazolidinoneSCHEMBL16620138epsilon-(gamma-Glutamyl)lysinechrysoplenol D2-Pentanone1-Carboxybiuret3'-Adenylic acidOrnithineN-Acetyl-D-glucosamine 6-phosphate1-Deoxy-11-oxopentalenate4,6-Dichloro-3-methylcatecholLigninIndoleDesethyl atrazineCMP-KDOMenatetrenoneIsocitric acid2,5-Dichloro-1,4-benzoquinonePlumbagin4,6-Dichloro-3-methyl-cis-1,2-dihydroxycyclohexa-3,5-dienefMLP;N-Formyl-MLFCyclopentolatePE(18_0_20_4(5Z,8Z,11Z,14Z))GabexateAllopurinolIsodiospyrinUric acidIndolepyruvateRhodoviolascinLinoleic acidNorchelerythrineStearic acid amideIsocyanocyclohexane4-Chlorobenzyl cyanidealpha-D-Galactosamine 1-phosphateChlorphenesin2-Deoxy-D-gluconateViolaceinDelphinidin 3-(6'-malonyl-glucoside)FA 20_2Vaccenic acidPropanoyl phosphate6-hydroxy-N-methylmyosmine(R)-2,3-Dihydroxy-3-methylvalerate(R)-3,3-dimethylmalic acid3-DehydrosphinganineSphingosineAlpha-ketobutyratePyridoxic AcidHallactone B2-hydroxylamino-4,6-dinitrotolueneN-Acetyl-L-Aspartic AcidThreonineMono-(2-ethylhexyl)phthalate12-Hydroxychelirubine2-Isopropyl-3-oxosuccinateXanthoxylolAcetaminophenLysineAICARL-Prolinamide4-KetocyclophosphamidePhenylacetaldehydeParathion2-Deoxyglucose 6-phosphateCannabidiolUridine diphosphategalactoseGuaninecis-Dihomoaconitic acidSterigmatocystin(25S)-3-oxocholest-4-en-26-alFructoselysine2-Keto-L-gluconateD-GalactoseAprepitantSelegilineIndole-3-CarboxaldehydeCiticolineN-hydroxy-4-aminobiphenylXanthosine(R)-2-hydroxystearic acid1,2-dihexadecanoyl-3-(9Z-hexadecenoyl)-sn-glycerolJusticidin BSulfamethizoleDexamethasone Sodium Phosphate(2R,3R,4R,5S,6R)-5-[(2R,3R,4R,5S,6R)-5-[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4S,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino]oxan-2-yl]oxy-3,4-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,4-triol6-HydroxydaidzeinHydroxymethylphosphonateomega-HydroxyphylloquinoneAtrazineMaleamic acidLevonorgestrelN-acetyl-alpha-D-hexosamine 1-phosphateMalvidin 3,5-diglucoside2,4-DINITROTOLUENETauroursodeoxycholic acidDihydroisopentenyldehydrorhodopinUDP-L-Ara4O4-HydroxycinnamaldehydeDe-O-methyldihydrosterigmatocystinNocodazoleDiketopiperazineGlutathione oxidizedHapalindole GSolanesyl-PP(22R,23R)-22,23-dihydroxy-campest-4-en-3-one(14E)-2,11-dihydroxy-14-(1H-imidazol-5-ylmethylidene)-9-(2-methylbut-3-en-2-yl)-2,13,16-triazatetracyclo[7.7.0.01,13.03,8]hexadeca-3,5,7,10-tetraene-12,15-dioneCarbamazepine-O-quinone2-OxazolidinoneCyclohexanMeglutol2E-Dodecenedioic acid1-deoxy-L-glycero-tetrulose 4-phosphate2-oxoglutaryl-CoADorzolamideAnandamideTaurohyocholic acidL-2-Amino-4-methylenepentanedioic acidMacheteMycinamicin III3-Methoxy-4-hydroxyphenylglycolaldehydeICI 1643844-Amino-2-hydroxylamino-6-nitrotolueneGuanidinopropionic acidcytidine-5'-diphosphateCarthamidin 6,7-diglucoside2,3-Bis-O-(geranylfarnesyl)-sn-glycerol 1-phosphate7-Methyluric acidb-D-Mannose 6-phosphateOxadiazon27-O-Demethylrifamycin SVMFCD00049038Aconitate [cis or trans]cyanidin 3-O-(6-O-glucosyl-2-O-xylosylgalactoside)Lolitrem Ebeta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine¦Ã-Aminobutyric acidalpha-D-glucose 1-phosphateLolitrem BThymineL-SorbosonateCytidine 3'-monophosphateDihydrocucurbitacin B(1R,4S,5R)-4-ThujanolGraveolinePsychotridineMaleicimideSarcosineMaltolsulfanitranN-Acetyl-7-O-acetylneuraminic acid16-Hydroxypalmitic acidTaurineGluconasturtiinOxyanin B3,5,6-Trichloro-2-pyridinolCoenzyme Q9Fenoxaprop-ethyl4-Chloro-2-methylphenolAmidosulfuronCyclotetraglucoseOryzalexin EAdenosine phosphosulfate3,4,6-TRICHLOROCATECHOLMedicocarpinAflatoxin-M1-8,9-epoxideLycoctonine3,4-DehydrorhodopinN-Acetyl-D-glucosamineRibonic acidVanilloyl glucose4-Chlorophenol20,21-Diprenylterpendole J20,21-Diprenylterpendole I5-phospho-alpha-D-ribose cyclic-1,2-phosphatePenicillin G20,21-Diprenylterpendole CDoconexentAdrenoyl ethanolamide3-(4-methylthio)butylmalateZoxamideJI-20Bcis-1,2-Dihydroxycyclohexa-3,5-diene-1-carboxylateL-TryptophanTriethanolamineGlucosamine 1-phosphateSwertiajaponinPinitolPhenylalanine4-Hydroxycoumarins3,5-DI-TERT-BUTYL-4-HYDROXYACETOPHENONEsecologaninLycorineUDP-N-acetyl-D-galactosaminuronic acidTauropine2-N-Acetyl-6-deamino-6-hydroxyparomomycin IICholesterolDhurrin2-epi-ValioloneOleanolic aldehyde6-Deamino-6-oxoparomomycin II5alpha-cholesta-8,24-dien-3-one(R)-demethyl-4-deoxygadusolUracilIminoaspartic acidadonixanthinAzatyrosineDinophysistoxin 1Creatine2-Hydroxybenzyl alcohol5-Aminolevulinic acidTaurocyaminphosphateCholesterol sulfateBehenic acid2-Deoxy-L-ribono-1,4-lactone2,5-dichloromuconic acidAminoundecanoic acidDihydro-O-methylsterigmatocystinSuccinic AcidCobalt-precorrin 4Guanidinosuccinic acidCyanuric acidPlastoquinone 9(E)-1-O-Cinnamoyl-beta-D-glucoseDecanoylcarnitine (C10)Acetic acid1-Deoxy-D-xylulose 5-phosphate5-HydroxyisourateHydroxanthommatin3-Oxoadipyl-CoA2-Oxovaleric acidQuercetin 3-O-xylosyl-rutinosidesteganacinpanaxynol2,4,5-trihydroxy-3-oxo-6-phosphonooxyhexanoic acidRibitol 5-phosphateS-(Hydroxymethyl)mycothiolNicotinic acid mononucleotidePhytanateD-Glucose 6-sulfateHarmalineL-Glutamic acidN,N-DimethylglycineGlycerol 2-phosphateCanrenone2,6-Dichloro-4-biphenylolThreonic acid5-Hydroxyeicosatetraenoic AcidGlyoxalButirosin Btrans-2,3-Epoxysuccinate2-Amino-a-carbolineNorepinephrine(R)-Methylphosphonofluoridic acid 1,2,2-trimethylpropyl esterGlutamine12-DehydrotetracyclineOligomycin5-Amino-6-ribitylamino uracilGamma-glutamyl-L-putrescinePseudohypericinTerpendole I5-Amino-6-(5'-phosphoribitylamino)uracilTerpendole JDexamethazone metasulfobenzoate sodiumBacampicillinBeiwutineHistidine2-METHYLFLUORANTHENEPhosphonoacetateSinapaldehydeVitamin KPC-M6SelenocysteineNAc-L5Y-amideDeoxyadenosine3-(2-Methylthio)ethylmalateCysteinylglycineBRODIFACOUMArachidonic AcidFormononetinLysine, DL-2-(6-methylthio)hexylmalateEpirizoleTMC 126gamma-butyrobetainyl-CoAalpha-AllocryptopineSolamargine1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine3-Amino-4-hydroxybenzoate1D-myo-Inositol 1,5-bis(diphosphate) 2,3,4,6-tetrakisphosphate2,3,6-TBALuteolinResolvin E1Atractyloside3-methylthiopropyl-desulfoglucosinolate4-SulfobenzoateMercuronXanthinegamma-PentachlorocyclohexeneCordycepin2-Chloro-6-hydroxy-1,4-benzoquinoneAla-Glu-OHAsparagine3,5-dibromocatecholubiquinone-82-Oxo-4-hydroxy-5-aminovalerateTricrozarin A2,3-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serineCefotiamAcetylcysteineN-Acetylmuramate2-CaffeoylisocitrateL-IsoleucineSesaminN-Carbamoylsarcosine7-DeoxyloganetinIsoalliinPipecolic acid, (-)-7-Methylthioheptyl glucosinolateUridine-Diphosphate-N-Acetylglucosamine2-Furoic acid3-Chloro-cis,cis-muconic acid10-Hydroxycarbazepine1-phospho-alpha-D-galacturonic acidPentanamideExemestane8-Oxo-dGMP2,4-dichloromuconic acidFormylisoglutamine2-ChlorophenolSedoheptuloseN-Formylmaleamic acidR.g.-Keto IIIPinobanksinMethyl oleateN-CyclohexylformamideBiperiden3,6-dichlorocatechol4-Hydroxybenzoate-O-glucosideCamalexinEpsilon-caprolactamTyrian purpleShikomolPiperidineUridine triphosphate(R)-SulcatolXylitol1D-myo-inositol 4-phosphate(2-)dolasetronEstronecrenatosideLumazinedTDP-alpha-D-forosamineUridine MonophosphateGlycerylphosphorylethanolaminePhosphoglycolic acidNebramycin factor 52'-Deoxyguanosine-5'-Diphosphate2-oxo-6-methylthiohexanoic acidUrolithin ADecaprenyl phosphateAdenosylhopaneCichoriin5-Amino-4-imidazole carboxylateChlorobenzeneTrans-4-hydroxyprolineOxopent-4-enoate6-Hydroxyhexanoic acidHarmanFucoxanthinBiotin9,11alpha-Epoxypregn-4-ene-3,20-dione2-HydroxyethylphosphonateTrilobineUridine Diphosphate Glucuronic AcidCysteineglutathione disulfideProstaglandin-c2Deoxycorticosterone(+)-threo-2-Amino-3,4-dihydroxybutanoic acidL-SorboseGlucosamineDicyclohexylamineQ27132134FA 4_2;O3Carbamic acid2-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinonePsychosine sulfatePantothenic AcidButyrylcarnitine2'-Deoxycytidine-5'-monophosphoric acid5-chlorobenzene-1,2,4-triolGuanosine monophosphateOleic acidCystathioninePheophytin aAlpha-Ketoglutaric AcidMevalonolactoneThialysine ketimine5,6-Indolequinone-2-carboxylic acidAdenylsuccinic acid2-Dehydro-3-deoxy-D-fuconateDMPPValidamine 7-phosphateN-formylmethionineEtaconazoleCyanidin 3-xylosyl(coumaroylglucosyl)galactosideD-glucose 1,6-bisphosphate3-Bromo-4-hydroxybenzoic acid5,6-DihydrothymineAlloepipregnanolone5-HydroxyconiferaldehydeDiethylphosphateGuanosine2,4-Dichloro-2,5-dihydro-5-oxofuran-2-acetate7-MercaptoheptanoylthreonineOctanoylcarnitine (C8)2,4,5-Trichlorophenoxyacetic acidPseudouridine 5'-phosphate1,3,7-Trimethyluric acidUridine-5'-DiphosphateChaps2-BromophenolN,N-DimethylsphingosineEdoxudineCholine AlfosceratedTDP-beta-L-rhodinose2-Chloro-2,5-dihydro-5-oxofuran-2-acetateHomocitric acidSulfamethazineUridineAvermectin B1b aglyconeCysteineIsophthalic acidCoM-S-S-CoBN-carbamoyl-beta-alanineOlivoretin DphosphonoacetaldehydeReduced coenzyme F4204'-PhosphopantothenoylcysteineL-Ascorbic aciddTDP-L-4-oxovancosamineN1-Acetyl-tabtoxinine-beta-lactamOxyquinoline3-Hydroxyl kyneurenineBacteriohopanetetrol cyclitol ether5-AcetamidovalerateNeoeriocitrinANTIBIOTIC A-31438Cycloheximide4-Hydroxy-3-octaprenylbenzoic acidGPEtn(16_0p_22_6(4Z,7Z,10Z,13Z,16Z,19Z))3-Hydroxy-6,7,8-trimethoxy-2-naphthoatealpha,alpha-Trehalose 6-mycolate1-NaphthylamineHypotaurineGlucoiberinGinsenoside RdIsospaglumic AcidSucrose2'-Deoxyguanosine 5'-monophosphateMelilotosidedTDP-4-oxo-5-C-methyl-L-rhamnosegamma-Glutamyl-alpha-aminobutyrateSpheroidenoneCannabisin EDimethyl sulphoneSpermidine1-Hydroxy-gamma-carotene glucoside2,5-Dioxopentanoatechloric acidtaxa-4(20),11-dien-5-alpha-yl acetateCyclo(tyrosyl-tyrosyl)4-hydroxy-4-methylglutamate5-MethylthioriboseEmindole SB7-Oxoheptanoic acid5-epi-Lividomycin BDioctyl phthalate3-deoxy-D-manno-octulosonate 8-phosphatePiperitenoneP 5184-AcetylbutyrateVanillylamineGinsenoside Rg1Ginsenoside Rg3Bacteriochlorophyll a5'-Methylthioadenosine4-NitrophenolO-ToluidinePiperineBenzimidazoleMethyloxaloacetateErucic AcidCreatinine5-O-(1-Carboxyvinyl)-3-phosphoshikimate4,4-Diapolycopenedialnonaprenyl-4-hydroxybenzoatePyridoxine6-Phosphogluconic acidRobenidineFormiminoglutamic acidCyproheptadinePaspalinine5-Aminopentanoic acidCyanidin 3-O-rutinoside 5-O-beta-D-glucosideMethyl selenacValine3-(6-methylthio)hexylmalateST 29_1;ODopamine quinoneErythronic acidGalantamine2-BromomaleylacetateSaccharolactonechlorohexadienedioic acidTheobromineMethanophenazine3-Iodo-4-hydroxyphenylpyruvateGlycerophosphoric acidKievitoneCDP-ethanolaminePterinLotaustralin1-Aminocyclopropane-1-carboxylic acid6-Imino-5-oxocyclohexa-1,3-dienecarboxylateCytidineS-adenosyl-4-methylthio-2-oxobutanoateGlucoerucinDemethyl-desacetyl-rifamycin SVL-Glutamic acid 5-phosphateIsoquinolineD-(-)-Gulonic acid gamma-lactoneAvermectin B1b monosaccharide3-Hydroxyisovaleric acidIsobutanal oximeaminopyrrolnitrinPyridoxine 5'-phosphateDodecanoic acidIsoprenyl alcoholTris(2-butoxyethyl) phosphateMycinamicin VISphinganinePhosphohydroxypyruvic acidDichloroacetatePelaninL-Xylofuranose[(R)-2,2,3-trimethyl-5-oxocyclopent-3-en-1-yl]acetic acidStearic AcidOleoylethanolamide4-Hydroxyphenylglyoxylic acidAklanonic acidLeukoefdinFesoterodine FumarateErythromycin CQuinate2,4,6-TrihydroxybenzophenoneFAL 16_1Premithramycin A2AllantoinGallopamil5-methylthiopentanaldoximeSpiroxamineD-Ribose 5-phosphateSCHEMBL1309076327-Deoxy-5b-cyprinolAtropineAcetosyringoneTaxifolinAjmalineLinolenic Acid4-Amino-4-cyanobutanoic acidGitoxinCrocetindialD-SorbitolMethyl glucosinolateDelphinidin 3-O-3,6-O-dimalonylglucosidePhytolMethionineBarbituric acidmethyl farnesoatePorphobilinogenTillmans Reagent(+)-GallocatechinMethyl RedDibenzothiophene 5-oxideAlbiflorin2-HydroxymuconateL-HistidinolCitrininCarnosine1,7-Diphospho-1-epi-valienolBROMOBENZENE(2E)-3-(2,4-Dihydroxyphenyl)prop-2-enoyl-CoAS-Formylglutathione3beta-hydroxy-5-cholestenoateN-METHYLANILINE4-HydroxymethylcatecholMyo-InositolNiacinamideDeoxyuridineDihydrozeatinPhenylacetylglycineGalactinolSecurinineO-Phospho-DL-serineInosinic AcidN-AcetylmannosamineHexylamineRacemethionineIxazomib Citrate2-O-(6-Phospho-alpha-mannosyl)-D-glycerateRifampinpiperidine-2-carboxamideD-4'-PhosphopantothenateStellata-2,6,19-trieneNorleucineNadideN-AcetylserotoninErythrodiolGlycylglycineAminocaproic acident-cassa-12,15-dien-2beta-ol4-Hydroxy-3-prenylbenzoateGuanosine 3'-diphosphate 5'-triphosphateBacteriohopanetetrol glucosaminePhytosphingosineAvermectin MS2,4-DichlorophenolMilitarinone A(R)-5-Diphosphomevalonic acidPropranololCitric AcidI-123 BMIPPNovapikromycinTyrosine17-DeoxycortoloneCoumarinQuinineL-Malic acidFustin16-DehydroprogesteroneMyxol 2-(2,4-di-O-methyl-fucoside)Spermine7,8-diaminopelargonate2-Hydroxycinnamic acid, (2E)-Hypoxanthine3-Chlorophenol2-Hydroxy-3-(4-hydroxyphenyl)propenoic acid2,4-DinitrophenolYatansinGlucocheirolinL-Argininegamma-Linolenic acidFO 2546M1-O-CaffeoylglucoseLevallorphan2-Arachidonoylglycerol6-HydroxyprotopineRhizocticin AMyristoleic AcidSulcatonedTDP-4-amino-2,3,4,6-tetradeoxy-D-glucosedammarenediol IIAtractyloside DD-Allose2-Amino-3-methylpentanoic acidN6,N6,N6-Trimethyl-L-lysineGalactitol 1-phosphate2,5-DichlorophenolOxaloglutarateOphthalmic acidGamma-delta-Dioxovaleric acidLignoceric acid (C24)2-keto-3-deoxy-D-glycero-D-galacto-nononate 9-phosphateLinuronD-SerineAdenine2-Deoxy-D-ribose 1,5-bisphosphate4-ChrysenecarboxylateCalcium Pantothenate7-Aminomethyl-7-carbaguanineCalcium;trisodium;2-[[2-[bis(carboxylatomethyl)amino]-3-(4-ethoxyphenyl)propyl]-[2-[bis(carboxylatomethyl)amino]ethyl]amino]acetateC16-CeramideD-ProlineDL-leucineTribromobisphenol AInosineN¦Á-Acetyl-L-lysineMycinamicin VIILumichromeOuabain5-MethylmaleylacetateGeissosperminePanthenol6-Chlorohydroxyquinol3-PhosphoadenylylselenateDelphinidin 3-sambubiosideN-Acetylmethionine4-(3-Hydroxy-2-naphthyl)-2-oxobut-3-enoic acidPirimicarbEllagic acid3-Thiomorpholinecarboxylic acidTetracenomycin F1 methylesterAvermectin B2b monosaccharide2,4,5-TrichlorphenolUDP-D-galacturonic acid6-Aminopenicillanic acidgamma-GlutamylcysteineCarbamazepineDimethylpropiothetinbeta-SitosterolL-4-Hydroxyglutamate semialdehydeBovinic acidtetrahomomethionine(¡À)-2-MethylbutanalUridine Diphosphate GlucoseTreosulfanAklaviketoneDodemorphTrithionatebeta-D-GALACTOPYRANOSE2-OxoarginineFurylfuramideChaksine5-Fluorouridine diphosphateDeoxyguanosineGingerolFA 12_3;O2Sinapic acid-O-glucosideDihydromacarpine2-hydroxypropyl-CoM13-HODE + 9-HODEPhosphodimethylethanolamineCytidine monophosphate4-Hydroxyphthalate7-Chlorodehydrotryptophan5-Acetylamino-6-formylamino-3-methyluracilCostunolideGluconic Acid3-(all-trans-octaprenyl)benzene-1,2-diolFlupenthixolN,N-Dimethylarginine2-[2-[[(2S)-2-amino-2-carboxyethyl]amino]-2-oxoethyl]-2-hydroxybutanedioic acidOxolinic acidTheasaponin F118-HydroxycorticosteroneBenzylpenicilloic acidLathosterol(1R)-Glutathionyl-(2R)-hydroxy-1,2-dihydronaphthalene(2S)-Deoxymyxol 2-alpha-L-fucosideTetrahydroxypteridineOxypurinolPhosphoribosyl-ATPCHEMBL1161469Phenyl hydrogen sulfatePropanal5'-DeoxyadenosineFAMPL-Fucono-1,5-lactone2,4-dihydroxy-2-heptenedioc acidLoxapineMaleic AcidRacecadotrilmonodechloroaminopyrrolnitrinL-2-Amino-3-oxobutanoic acidAllantoic acidAzelaic acidMycothiolomega-Sulfo-beta-dihydromenaquinone-99,10-Epoxystearic acidPalmitic AcidDelphinidin 3-O-(6''-O-malonyl)-beta-D-glucoside-3',5'-di-O-beta-D-glucosidealpha-D-GlucoseCitalopram aldehydepregnan-21-alChrysophanol26-hydroxybrassinolideUNC0638CelecoxibPhosphocreatine2,3,7,8-Tetrachlorodibenzo-p-dioxin2-Hydroxy-2-ethylsuccinic acid2,5-Furandicarboxylic acidalpha-(1,2-Dihydroxyethyl)-1,2,3,4-tetrahydro-7-hydroxy-9-methoxy-3,4-dioxocyclopenta[c][1]benzopyran-6-acetaldehydeSynephrineST 29_2;O3PsoralenPepstatin3-sulfolactaldehydeLysoSM(d18_1)NorvalineTrigonelline (N'-methylnicotinate)falseUntargeted LC-MS metabolomic comparison of canine osteosarcoma McKinley cells vs normal PEKCC2382 osteoblast cellsUntargeted LC-MS metabolomics analysis was performed on McKinley canine osteosarcoma cells (Group A) and PEKCC2382 normal canine osteoblast cells (Group B). This study screens characteristic differential metabolites and elucidates distinct metabolic phenotype discrepancies between malignant osteosarcoma and physiological osteoblast cells in dogs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