MS/MS and MS mass spectrometry information were matched with metabolite databases. MS mass error was set to less than 10 ppm. Meanwhile, metabolites were identified according to secondary mass spectrometry matching scores. The main databases were mainstream public databases such as http://www.hmdb.ca/ and https://metlin.scripps.edu.edu/, as well as the self-built database of Shanghai Majorbio Bio-pharm Technology Co., Ltd.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Column specification was ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 µm). Mobile phase A was consisted of 5 % acetonitrile + 95 % water (containing 0.1% formic acid), and mobile phase B was consisted of 47.5% isopropanol + 47.5% acetonitrile + 5% water (containing 0.1% formic acid). The injection volume was 3 μL, and the column temperature was set to 40 °C.
"],"publication":["Probiotic properties of Pediococcus acidilactici Z123 and its intervention effects and mechanisms on hypercholesterolemia in mice."],"submitter_affiliation":["Peking University"],"submitter_name":["Duo Keai"],"organism_part":["Liver"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["30 ± 5 mg of liver tissue was accurately weighed into a 2.0 mL centrifuge tube, and a grinding bead with a diameter of about 6 mm was added. Prepare an extract with a volume ratio of water to methanol of 1:4, take 200 µL, and add four internal standards such as L-2-chlorophenylalanine (0.02 mg/mL). Grind at 50 Hz and 10 °C for 6 min on a frozen tissue grinder, then extract by low-temperature ultrasound at 40 kHz and 5 °C for 30 min.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14733"],"author":["Jinsong Wu. Henan University of Animal Husbandry and Economy. 413756821@qq.com.","Jian Zou. Henan University of Animal Husbandry and Economy. zoujianzz@126.com."],"data_transformation_protocol":["The raw data were imported into metabolomic processing software Progenesis QI v3.0 (Waters Corporation, Milford, USA) for baseline filtering, peak identification, integration, retention time correction and peak alignment, and finally a data matrix containing information such as mass-to-charge ratio, retention time and peak intensity was obtained. Then the software was used to search and identify characteristic peaks.
"],"study_factor":["Group"],"submitter_email":["keaiduoduo998@126.com"],"sample_collection_protocol":["Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver Liver
"],"omics_type":["Metabolomics"],"study_design":["Pediococcus acidilactici Z123","high cholesterol and hyperlipidemia","Mus musculus","signaling pathways","AB Triple TOF 6600","Probiotic properties","metabolomics","untargeted analysis","Liver","lipid metabolism","Agilent 1290 Infinity","liver tissue"],"curator_keywords":["high cholesterol and hyperlipidemia","Pediococcus acidilactici Z123","Mus musculus","signaling pathways","AB Triple TOF 6600","metabolomics","Probiotic properties","untargeted analysis","Liver","lipid metabolism","Agilent 1290 Infinity","liver tissue"],"mass_spectrometry_protocol":["The sample was ionized by electrospray ionization, and mass spectrometry signals were collected in positive and negative ion scanning modes respectively. Specific conditions were as follows: scanning range 70–1050 m/z, sheath gas flow rate 50 arb, auxiliary gas flow rate 13 arb, heating and capillary temperature set to 425 °C and 325 °C. Spray voltage positive and negative modes were 3500 V and 3500 V, respectively, and the collision energy were 20, 40, 60 eV.
"],"additional_accession":[]},"is_claimable":false,"name":"Probiotic properties of Pediococcus acidilactici Z123 and its intervention effects and mechanisms on hypercholesterolemia in mice","description":"The intervention effects and underlying mechanisms of Lactobacillus strains against hypercholesterolemia in mice have been extensively documented. However, fewer studies have focused on the cholesterol-lowering potential and the corresponding metabolic regulatory mechanisms of Pediococcus acidilactici. In the present study, we first characterized the in vitro probiotic properties of a novel lactic acid bacterial strain, Pediococcus acidilactici Z123 (P. acidilactici Z123), which exhibited prominent cholesterol-lowering activity. Subsequently, intragastric administration of P. acidilactici Z123 was carried out in a mouse model of high-fat diet-induced hypercholesterolemia to assess its intervention effect and elucidate the underlying molecular mechanisms. The results revealed that P. acidilactici Z123 possessed desirable probiotic characteristics such as auto-aggregation, adhesion ability, and gastrointestinal fluid tolerance, and showed good biosafety, including antibiotic susceptibility and no hemolytic activity. In vivo results demonstrated that supplementation with P. acidilactici Z123 effectively alleviated excessive body weight gain and reduced visceral fat accumulation, improved serum lipid profiles, and ameliorated the histological morphology of the liver and epididymal adipose tissue. Untargeted metabolomics indicated that P. acidilactici Z123 exerted a significant regulatory effect on lipolysis in adipocytes, PPAR and AMPK signaling pathways, primary bile acid metabolism, bile secretion, tryptophan metabolism, etc. Notably, such metabolic regulation was further verified at the transcriptional level. Quantitative reverse transcription PCR (qRT-PCR) analysis revealed that P. acidilactici Z123 intervention could inhibit cholesterol synthesis by downregulating the expression of Hmgcr and Srebf1, and upregulating the expression of Prkaa1. Meanwhile, it also elevated the expression of genes related to fatty acid oxidation (Cpt1a, Pparalpha) and bile acid synthesis (Cyp7a1). All these molecular changes jointly modulated systemic lipid metabolism. The present findings will provide valuable theoretical evidence for the development and application of novel functional probiotic products with lipid-regulating properties.
","dates":{"publication":"2026-06-13","submission":"2026-06-09"},"accession":"MTBLS14733","cross_references":{}}