The identified metabolites were annotated using the KEGG database (https://www.genome.jp/kegg/pathway.html), the HMDB database (https://hmdb.ca/metabolites) and the LIPIDMaps database (http://www.lipidmaps.org).
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) analysis was used to quantify metabolites using a Vanquish UHPLC system (Thermo Fisher, Germany) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher, Germany). Samples were injected into a Hypesil Gold column (100 x 2.1 mm, 1.9 μm) at a 0.2 mL/min flow rate using a 17-min linear gradient. The eluents for the positive polarity mode were Eluent A (0.1% formic acid aqueous solution) and Eluent B (methanol). The eluents for the negative polarity mode were Eluent A (5 mM ammonium acetate, pH 9.0) and Eluent B (methanol). The solvent gradient was set as follows: 2% B, 1.5 min; 2-85% B, 3.0 min; 85-100% B, 10.0 min; 100-2% B, 10.1 min; 2% B, 12.0 min.
"],"publication":["Berberine improves intestinal barrier and cognitive impairment by remodeling intestinal microbiota in C57BL/6 septic mice induced by LPS."],"submitter_name":["chaohui zeng"],"submitter_affiliation":["North Sichuan Medical College"],"organism_part":["intestine"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Tissues (100 mg) were individually grounded with liquid nitrogen and the homogenate was resuspended with prechilled 80% methanol by well vortex. The samples were incubated on ice for 5 min and then were centrifuged at 15,000 g, 4°C for 20 min. Some of supernatant was diluted to final concentration containing 53% methanol by LC-MS grade water.The samples were subsequently transferred to a fresh Eppendorf tube and thenwere centrifuged at 15000 g, 4°C for 20 min. Finally, the supernatant was injected into the LC-MS/MS system analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14775"],"author":["chaohui zeng. North Sichuan Medical College. 15881316900@163.com."],"data_transformation_protocol":["The data files generated by UHPLC-MS/MS were processed by XCMS to perform peak alignment, peak picking, and quantitation for each metabolite. Then, based on adduct ions and setting mass deviation to 10 ppm, a comparison was made between these data and the high-quality secondary spectrum database to obtain results for metabolite identification. After eliminating background ions according to blank samples, the original quantitative results were normalized by the following formula to obtain relative peak areas: Relative peak areas = Raw quantitative value of samples/ (The sum of quantitative value of samples/ The sum of quantitative value of QC1); Compounds with a coefficient of variation (CV) of relative peak areas in QC samples greater than 30% were removed. Finally, the identification and relative quantitation results of metabolites were obtained. Data processing is based on the Linux operating system (CentOS version 6.6), using R and Python.
"],"study_factor":["Treatment"],"submitter_email":["15881316900@163.com"],"sample_collection_protocol":["After feeding LPS induced C57BL/6 septic mice with berberine, we performed behavioral, HE staining, ELISA, western blot, metagenomic sequencing of gut microbiota and untargeted metabolomics analysis to evaluate the effects of berberine on septic mice.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Mus musculus","Thermo Scientific software","intestine","Thermo Scientific Q Exactive","untargeted analysis","OpenMS","XCMS","experimental sample"],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","Metabolomics","Mus musculus","Thermo Scientific software","intestine","Thermo Scientific Q Exactive","untargeted analysis","XCMS","OpenMS","experimental sample"],"mass_spectrometry_protocol":["The Q Exactive HF mass spectrometer was operated in positive/negative polarity mode with a spray voltage of 3.5 kV, capillary temperature of 320 °C, sheath gas flow rate of 35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heater temperature of 350 °C.
"],"additional_accession":[]},"is_claimable":false,"name":"Berberine improves intestinal barrier and cognitive impairment by remodeling intestinal microbiota in C57BL/6 septic mice induced by LPS","description":"After feeding LPS induced C57BL/6 septic mice with berberine, we performed behavioral, HE staining, ELISA, western blot, metagenomic sequencing of gut microbiota and untargeted metabolomics analysis to evaluate the effects of berberine on septic mice.","dates":{"publication":"2026-06-17","submission":"2026-06-17"},"accession":"MTBLS14775","cross_references":{}}