MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14783/m_MTBLS14783_LC-MS_alternating_normal-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14783/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14783/s_MTBLS14783.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14783/a_MTBLS14783_LC-MS_alternating_hilic_metabolite_profiling.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14783<p>Quantitation of metabolites in each assay module was achieved using multiple reaction monitoring of calibration solutions and study samples with isotopically-labelled internal standards&nbsp;</p>MetaboLightsPublicLiquid Chromatography MS - alternating - hilic<p>Metabolites were analyzed with a unique LC/MS/MS method to optimize their chromatographic resolution and sensitivity. Quantitation of metabolites in each assay module was achieved using multiple reaction monitoring of calibration solutions and study samples with isotopically-labelled internal standards on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer.</p>Tryptophan degradation by intestinal Bacteroides induces anti-tumor immunity and limits melanoma growth. 10.64898/2026.05.06.723300.ZEEV RONAICEDARS SINAI MEDICAL CENTERcecummass spectrometry assay<p>Cecal samples (~5 mg) were homogenized in 500 ul acetonitrile with 0.3% formic acid</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14783ZEEV RONAI. CEDARS SINAI MEDICAL CENTER. 121 N San Vicente Beverly Hills, CA, 90211 USA. ZEEV.RONAI@CSMC.EDU.<p>Raw data was processed using Mass Hunter quantitative analysis software (Agilent). Calibration curves (R2 = 0.99 or greater) are either fitted with a linear or a quadratic curve with a 1/X or 1/X2 weighting.</p>Cecal bacterial groupzeev.ronai@csmc.edu<p>Cecal contents samples from mice with varied microbiota were collected, flash frozen and stored frozen before extraction</p>MetabolomicsMetabolomicsMus musculusAbramson Cancer Centertargeted analysisCecumOpenMS1290 Infinity 2D-LCmelanomaAgilent 1290 Infinity UHPLCexperimental sampleMetabolomicsMus musculusAbramson Cancer Centertargeted analysisCecumOpenMS1290 Infinity 2D-LCmelanomaAgilent 1290 Infinity UHPLCexperimental sample<p>Metabolites were analyzed with a unique LC/MS/MS method to optimize their chromatographic resolution and sensitivity. Quantitation of metabolites in each assay module was achieved using multiple reaction monitoring of calibration solutions and study samples with isotopically-labelled internal standards on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer.</p>glutamic acidalaninefalsemetabolomics Analysis in Cecal SamplesTargeted LC/MS Metabolomics. Cecal contents were lyophilized overnight and approximately 2.5-5 mg was homogenized in 250 µL of 50% acidified acetonitrile (0.3% formic acid) for targeted LC/MS/MS metabolomics (acylcarnitines, amino acids, organic acids, nucleotides, and CoAs) according to validated, optimized protocols in our previously published study (Previs et al.). Separate aliquots of homogenates were extracted with solvents for each class of metabolites, and then each class was analyzed with a unique LC/MS/MS method to optimize their chromatographic resolution and sensitivity. Quantitation of metabolites in each assay module was achieved using multiple reaction monitoring of calibration solutions and study samples with isotopically-labelled internal standards on an Agilent 1290 Infinity UHPLC/6495 triple quadrupole mass spectrometer. Raw data was processed using Mass Hunter quantitative analysis software (Agilent). Calibration curves (R2 = 0.99 or greater) are either fitted with a linear or a quadratic curve with a 1/X or 1/X2 weighting. Metabolic and Proteomic Defects in Human Hypertrophic Cardiomyopathy Michael J. Previs, Thomas S. O’Leary, Neil B. Wood, Michael P. Morley, Brad Palmer, Martin LeWinter, Jaime Yob, Francis D. Pagani, Christopher Petucci, Min-Soo Kim, Kenneth B. Margulies, Zoltan Arany, Daniel P. Kelly, Sharlene M. Day bioRxiv 2021.08.18.455967; doi: https://doi.org/10.1101/2021.08.18.4559672026-06-192026-06-18MTBLS14783MTBLC16449CHEBI:16449