MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/m_MTBLS14800_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/m_MTBLS14800_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/a_MTBLS14800_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/a_MTBLS14800_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800/s_MTBLS14800.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14800At the same time, MS and MS/MS mass spectrometry data were matched against the public metabolite databases HMDB (http://www.hmdb.ca/) and Metlin (https://metlin.scripps.edu/), as well as Meiji’s in-house database, to obtain metabolite information.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseThe instrument platform used for this LC-MS analysis was Thermo Fisher’s ultra-high-performance liquid chromatography-tandem Fourier transform mass spectrometry UHPLC-Orbitrap Exploris 240 system (Shanghai Meiji Biomedical Technology Co., Ltd.). Chromatographic conditions: A 3 μL sample was separated on an HSS T3 column (100 mm × 2.1 mm i.d., 1.8 µm) before entering the mass spectrometer for detection. Mobile phase A consisted of a water/acetonitrile (95/5, v/v) solution containing 0.1% formic acid; mobile phase B consisted of an acetonitrile/isopropanol/water (47.5/47.5/5, v/v/v) solution containing 0.1% formic acid. The flow rate was 0.40 mL/min, and the column temperature was 40.Novel Adaptive Strategies of Maize Rhizosphere Microbiome Mediated by strain ST-1 Against Vanadium Stress.chengdu university of technologyXinpeng Wangrhizospheremass spectrometry assayPlace 100 mg of solid sample in a 2 mL centrifuge tube and add one 6 mm diameter grinding bead. Add 800 μL of extraction solution (methanol:water = 4:1 (v:v)), containing four internal standards (L-2-chlorophenylalanine (0.02 mg/mL), etc.), for the extraction of metabolites. Grind the sample solution in a frozen tissue grinder for 6 minutes (-10, 50 Hz), followed by low-temperature ultrasonic extraction for 30 minutes (5, 40 kHz). Allow the sample to stand at -20 for 30 minutes, then centrifuge for 15 minutes (4, 13,000 g). Transfer the supernatant to a sample vial with an insert tube and load it into the instrument for analysis.Maize Rhizosphere Microbiomehttps://www.ebi.ac.uk/metabolights/MTBLS14800Xinpeng Wang. chengdu university of technology. 3514364342@qq.com.Yi Huang. Chengdu university of technology. huangyi@cdut.edu.cn.After the analysis was completed, the raw LC-MS data were imported into the metabolomics analysis software Progenesis QI (Waters Corporation, Milford, USA) for baseline filtering, peak identification, integration, retention time correction, and peak alignment, ultimately yielding a data matrix containing retention times, mass-to-charge ratios, and peak intensities,Group3514364342@qq.comThe sample was collected from the rhizosphere soil of a corn pot experiment in the experimental greenhouse of Chengdu University of Technology in Chengdu, Sichuan Province, China, in July 2025. After collection, the sample was rinsed with pure water and sent to Shanghai Meiji Biological Testing Co., Ltd. for testing. The sample was stored in a -80°C refrigeratorMetabolomicsMetabolomicsMetagenomicsSCIEX ExionLC ADuntargeted analysisMaizesoilRemediationUHPLC -Orbitrap Exploris 240Vanadium stressMaize Rhizosphere MicrobiomerhizosphereMetabolomicsMetagenomicsSCIEX ExionLC ADuntargeted analysisMaizesoilRemediationUHPLC -Orbitrap Exploris 240Vanadium stressMaize Rhizosphere MicrobiomerhizosphereSample mass spectrometry signal acquisition was performed in positive and negative ion scan modes, with a mass scan range of m/z 70–1050. Ion spray voltage: 3500 V for positive ions and –3000 V for negative ions; sheath gas 50 arb; auxiliary heating gas 13 arb; ion source heating temperature 450; collision energy cycle 20–40–60 V.3-Hydroxy-2,6,6-Trimethylcyclohexene-1-Carboxylic AcidBk-MddmaDocosanol3-Methylglutaconic AcidFeruloyl TyramineKaempferol 3-[2''-(6'''-Coumaroylglucosyl)-Rhamnoside] 7-GlucosideXestoaminol CCellopentaose4-O-Methyl-12-O-Tetradecanoylphorbol 13-AcetateN1,N10-Dicoumaroylspermidine(24S)-24-EthylbrassinoneN,N-Dimethyltetradecylamine-N-Oxide3-Amino-2-Methoxynonadec-5-En-4-OlCaffeic AcidCis-4-HeptenalHistidylprolineChlorobenzeneProstaglandin H28-Methylsulfinyloctyl IsothiocyanateImidazolepropionic AcidPaxillineN,N-Dimethyloctanamide12-Hydroxy-13-(Hydroxymethyl)-3,5,7-Trimethyl-2,4-Tetradecadienedioic AcidN-Methylanthranilic Acid(+/-)-(Z)-2-(5-Tetradecenyl)Cyclobutanone3H-Sialic AcidIngenol4-Acetylbenzo[D]Oxazol-2(3H)-OneFurfuralGingerglycolipid BErigeside CHexamethoxymethyl MelamineAntibiotic Ll-Ab 664Cyclo(His-Pro)2-Fluoro-6-(4-Methoxyphenoxy)Benzonitrile7-Tert-Butyl-N-(Thiophen-2-Ylmethyl)Pyrazolo[1,5-A]Pyrimidine-5-CarboxamideVal-PheMelamineN-Cyclohexanecarbonylpentadecylamine6-SulfanilamidoindazoleGln-Leu4-MethylstyreneFarnesolVal-Tyr-ArgOleamideSulfometuron-MethylSpb(20:0_2O)10-NitrooleateTyr-SerTrimethoprim(Z)-9-Cycloheptadecen-1-OneBetalamic AcidNeomangiferinBis-(Acetyl)DiaminopentanePro-His4,5-Seco-DopaIsosilybinPc(Pgf2Alpha/16:1(9Z))1-[3-(4-Chlorophenoxy)Propyl]-2-Methyl-4-Nitroimidazole2-HydroxyatrazineLysylglutamic AcidCortisol 21-Sulfate3-(5-Hydroxy-2,3-Dimethyl-3-Tricyclo[2.2.1.02,6]Heptanyl)Propanoic AcidGanodermic Acid Tq7-O-MethylbavachinMasitinib2-Amino-9-[(4S)-4-Hydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]-1H-Purin-6-One4,4'-DioctyldiphenylamineMoracetinNegletein 6-[Rhamnosyl-(1->2)-Fucoside]D-GlucoseOctyl Methoxycinnamate1-(Tert-Butylamino)-3-(3-Fluorophenyl)Thiourea9-OxoodeArg-ProN-ArachidonylethanolamineProstaglandin A2Ile-GluDg(Pgf1Alpha/0:0/I-21:0)4-Oxo-2-NonenalPhthalic AnhydrideArg-Ser-ProSialorphinThr-AlaL-CarnitineEmetineAlpha-IononeN-Lauroylglycine(+/-)8-GingerolArachidyl Alcohol3-Methyl-2-ButenalDeoxyandrographolideAdenosineN,N-DiisopropylethylamineDg(8:0/22:6)OxyquinolineIle-Val-AsnPhenamilTetradecyldiethanolaminePc(18:2/0:0)Patulin4-Morpholinepropanesulfonic Acid1-(3-Carboxypropylcarbamoyl)-5-FluorouracilBis(2-Ethylhexyl) PhthalateMgdg(O-22:6/7:0)Glucose Lactate AcetateAcetoacetic AcidL-TyrosineNeolinustatinHydroxy Bosentan4-Methyl-1H-BenzotriazoleDimethyl PhthalateMyotoxin A7-[2-(1-Hydroxyhexyl)-3,6-Dihydro-2H-Pyran-6-Yl]Heptanoic Acid2-Benzylsulfanyl-N-[[1-(Hydroxymethyl)Cyclopropyl]Methyl]Acetamide4-Hydroxy-4-(3-Hydroxy-1-Butenyl)-3,5,5-Trimethyl-2-Cyclohexen-1-OneDg(Pge2/I-18:0/0:0)DodecanamideL-PhenylalaninePa(Pgj2/I-16:0)4Alpha-Phorbol 12,13-DidecanoateTrihexyphenidylLauramine OxideSpb(14:0_2O)Rose Oxide (Cis)11,13-DehydroeriolinCarbendazimScytaloneNeu5Nacalpha2->6LactoseCephamycin CMethenamine(S)-(-)-Perillyl AlcoholMelengestrol AcetateO-Succinyl-L-HomoserineUrocanic AcidSclareolideLys-Lys-ThrMaltol PropionatePterosin GDimethylformamideBeta-IononeHdmboa-GlcSm(8:1_2O/24:6)Gamma-Glutamylvaline6-Gingerol(E)-Heptadec-6-En-16-Yne-1,2,4-Triol3-MethoxyanthranilateGuanine2'-Deoxy-7,8-Dihydro-8-OxoadenosineNivalenolOctadecanamideSpb(22:0_2O)Loganate(Z)-3-(4-Oxo-5-Propan-2-Ylidene-2-Propylfuran-3-Yl)Prop-2-Enoic AcidFlunisolideIndole-3-CarboxaldehydeDesmethyl Bosentan7-O-Methyl Morroniside(2S,3R,4S,5S,6R)-2-(3,5-Dihydroxyphenoxy)-6-(Hydroxymethyl)Oxane-3,4,5-TriolPyridoxine3-O-P-Trans-Coumaroylalphitolic AcidAristospanAlpha-Amino-3-Hydroxy-5-Methyl-4-Isoxazolepropionic Acid(S)-2,3-Dihydro-5-Hydroxy-2-Methyl-1,4-Naphthoquinone3,4-Dihydro-6,8-Dihydroxy-3-(10-Hydroxyundecyl)IsocoumarinGamma-TerpineneDifructose Anhydride IiiMonnieriside G2-Aminoheptanedioic Acid5'-Deoxy-5'-MethylthioadenosineThr-GlyDocosanamideTris(2,4-Di-Tert-Butylphenyl) PhosphiteCucurbic AcidArachidoyl EthanolamideDg(O-15:0/20:5)Plantamajoside3-Methyl-1-ButylamineCoroninCinobufaginPa(Pgd1/I-14:0)Betaine2-Azaspiro[4.5]Decan-3-One(10Z,14E,16E)-10,14,16-Octadecatrien-12-Ynoic AcidN-Acetyl-S-(2-Carbamoylethyl)-L-Cysteine9,12-Octadecadiynoic AcidOxymetholonePipecolic AcidN-Epsilon-Acetyl-L-LysineAntibiotic X 14889ALotaustralin6-Methyl-7-Nitro-2,3-Dihydro-1,4-BenzodioxineGinkgetinGinsenoside Rh5N-DesmethylvenlafaxineAnandamideGlutamic AcidVulgarinAustinoneol3-Hydroxy-7-Methoxy-3H-2-Benzofuran-1-One2-HeptadecylfuranMilbemycin Beta2Feruloyl Quinic Acid2-(Hydroxymethyl)-3-Methoxy-2H-Furan-5-OneCyclopassifloic Acid BN-Acetyl MesalazineEmmolic AcidDeoxycholylserineGermacrene DCer(8:1_2O/5:0)Coumaroyl Tyramine(9Z,12Z)-7,8,16-Trihydroxyoctadeca-9,12-Dienoic Acid3-KetosphingosineHistidinohydroxylysinonorleucineMethyl(S)-3-Hydroxybutyrate22-Hydroxydocosanoic AcidArg-Ala-TyrN-Stearoyl ValineErythromycin CBornyl FerulateDeoxycholylarginineCorylinCamphor4-Methylphthalic Anhydride4-OxofenretinideSitosterol 3-O-(6'-O-Stearyl-Beta-D-Glucoside)Cis-Uvariamicin IbVelvioneFeruloyl O-Methyldopamine(6E,8E)-5,10-Dioxooctadeca-6,8-Dienoic AcidPteroside ZEphedrine(5Alpha,6Beta,14Alpha,20R,22R)-5,6,14,20,27-Pentahydroxy-1-Oxowith-24-EnolideJanthitrem F3,6-Dibenzyl-9,12-Di(Propan-2-Yl)-1,4,7,10-Tetrazacyclododecane-2,5,8,11-Tetrone1-[(4-Amino-3-Methylphenyl)Methyl]-5-(2,2-Diphenylacetyl)-6,7-Dihydro-4H-Imidazo[4,5-C]Pyridine-6-Carboxylic AcidTroxerutinDibutyl SebacateDelta17-6-Keto Prostaglandin F1AlphaQuercetin 3-O-Xylosyl-RutinosideDesmethylmisonidazoleMorusinJaeschkeanadiol(2'E,4'Z,7'Z,8E)-Colnelenic AcidGlutamylglutamic AcidTrigonellinePhytol3-Hydroxy-5,5,8A-Trimethyl-3,4,4A,6,7,8-Hexahydronaphthalene-2-Carboxylic AcidHexylitaconic Acid F5-Chloro-N-[2-(3-Ethylimidazo[4,5-B]Pyridin-2-Yl)Ethyl]-2-FluorobenzamideStyrene2-Amino-3-Methoxybenzoic Acid9-OxootreButylisopropylamineSucrose-6-PhosphateGeranylgeraniolOphiopogonoside AN-Octyl-2-PyrrolidoneL-4-ChlorotryptophanPe-Nme2(9D3/11M3)2,4-Dihydroxy-2,5-Dimethyl-3(2H)-FuranoneSoyasapogenol ELauryldiethanolamine4-Amino-1-[(2S,4S)-2-(Hydroxymethyl)-1,3-Oxathiolan-4-Yl]Pyrimidin-2-One(5Xi,6Beta,9Xi,13Xi,18Xi)-6-Hydroxy-3-Oxolup-20(29)-En-28-Oic AcidL-TryptophanGanoderic Acid EpsilonThiamethoxamFlavone Base+3O,2Meo,O-Guaiacylglyceryl-HexPro-ArgN-Eicosapentaenoyl TryptophanBis(2-Ethylhexyl)AdipateAspirinTyr-Val-Leu1-(Cyclopropanecarbonyl)-N-(3-Phenylpropyl)-2,3-Dihydroindole-5-Carboxamide1-(2,3,8,8-Tetramethyl-1,2,3,4,5,6,7,8-Octahydronaphthalen-2-Yl)EthanoneTrans-Zeatin-D5Cinncassiol C3ThiobutabarbitalTetrahydropterinEstraneGluten Exorphin CPaullinic AcidLeucylprolineDg(Pgf1Alpha/0:0/I-16:0)HexylamineAcetyltributyl CitrateVal-Leu-Pro-Val-ProVidarabineFarnesyl AcetonePhloroglucinolGln-Phe-Arg5-(3,4-Dimethoxyphenyl)-3,4-Dimethyloxolan-2-OneCreatine(E)-6-Hydroxy-2,7-Dimethyloct-2-Enedioic AcidOxandroloneAnnonacinPhe-Ser-ArgFeruloyl AllylamineIsosteviol1-Nitro-5,6-Dihydroxy-DihydronaphthaleneCytosineBeauvericinAtratoneKuwanon G12-Hydroxyicosanoic Acid6-Hydroxy-2-AminopurineBrassinolide5,7-Megastigmadien-9-Ol GlucosideHypoxanthineAsp-Lys-LysRocuroniumNotoginsenoside R2Vernolic AcidNonadecylresorcinol GlucuronideThermoactinoamide A3,4-Dihydroxyphenylacetic AcidThermoactinoamide EIndole-3-Lactic AcidArmillatin2-Pyrimidine Acetic Acid2,6-Diamino-9-(2-Hydroxyethoxymethyl)PurineDifenoconazolePalmitoleic AcidAdenine13-Hydroxy-5'-O-Methylmelledonal4-(Hydroxymethyl)-3-(1-Hydroxy-5-Methylhexyl)Oxolan-2-OneInosineSpb(16:1_2O)N-Fructosyl IsoleucineSer-Arg-Pro3,5-Dihydroxy-6-Methyl-2,3-Dihydro-4H-Pyran-4-OneDg(Pge1/I-18:0/0:0)1-Dodecyl-2-PyrrolidinoneSoblidotinP-TolualdehydeIndolophenanthridine2,5-DimethylbenzaldehydeN-Acetyl-L-Glutamate 5-Semialdehyde7A,12A-Dihydroxy-3-Oxo-4-Cholenoic AcidN-(3-Methoxybenzyl)Palmitamide3-Hexenedioic AcidPhysalin MDeoxyadenosineBis(2-Ethylhexyl) SebacateN-Oleoyl ArginineDecylamine Oxide(6E,8E)-3-Hydroxy-10-Methoxy-4,9-Dimethyl-10-Oxodeca-6,8-Dienoic AcidGlycerophospho-N-Arachidonoyl EthanolamineDg(Pgd1/I-16:0/0:0)Dodemorph6-HydroxymelatoninZeaxanthin1,2,3,4-Tetramethyl-1,3-CyclopentadienePiperidinesParamethasoneHeptaethylene Glycol Monododecyl EtherCholine GlycerophosphateJurubineProstaglandin Pge2 1-Glyceryl EsterGlaucarubin20-Oxo-Leukotriene E4AlanylisoleucineOctadeca-10,12-Dienoic AcidMesembrinol5-(Hydroxymethyl)-4-Methoxy-2(5H)-FuranoneCholine2-[Bis-(2-Hydroxy-Ethyl)-Amino]-2-Hydroxymethyl-Propane-1,3-DiolTetradeca-9,12-Dien-1-Ol9-Octadecynoic Acid2-Amino-2-Methylpropan-1-OlAnisatinRubschisandrin3Alpha,4,7,7Alpha-Tetrahydro-4-Hydroxy-1H-Isoindole-1,3(2H)-DioneCoronaric AcidIndolinePregnanetrioloneNandrolone2-Cyclotetradecen-1-OneBicyclo Prostaglandin E22'-Deoxy-2'-Methylenecytidine4-Methoxy-6-Pentyl-2-Prop-1-En-2-Yl-2,3-Dihydro-1-Benzofuran-7-Carboxylic AcidLaurolactamLicoriconeErucamideCis-7-Hexadecenoic AcidNicotinic AcidN-[(2-Chloro-6-Fluorobenzyl)Oxy]-2-Cyano-3-(Dimethylamino)AcrylamideHeptadecasphinganine5-DeoxymyricanoneCholine SulfateMangalkanyl GlucosideLevan NHexadecanamideEleutheroside BRhodamine 6GN-Phenethyl-3-Phenyl-AcrylamideLinoleoyl Ethanolamide3-(6-Methylheptyl)-3,6-Dihydro-1H-Furo[3,4-C]Furan-4-One7,15-Dihydroxyabieta-8,11,13-Triene-18-Oic AcidGanoderic Acid K6-HydroxyheptanoylcarnitineDiethyltoluamide5,8-Dihydroxy-1,4-NaphthoquinoneTolueneFilipin IiMaltohexaoseDesoxylimonin4-HeptylphenolDihydrocaffeic AcidPentaethylene GlycolErysenegalensein J1,3-DiphenylguanidineSonchuionoside CCichorioside DNorvaline28-HomobrassinolidefalseNovel Adaptive Strategies of Maize Rhizosphere Microbiome Mediated by strain ST-1 Against Vanadium StressVanadium (V) contamination can disrupt plant growth, rhizosphere redox processes, and microbial functions, but how functional bacterial inoculation coordinates rhizosphere ecological adaptation with V behavior in crop systems remains unclear. In this study, a maize pot experiment was conducted to systematically investigate the strain ST-1-mediated rhizosphere response to V(V) stress by integrating plant physiological traits, V accumulation and fractionation, 16S rRNA sequencing, untargeted metabolomics, and metagenomics. V stress inhibited maize growth and induced oxidative damage, whereas strain ST-1 inoculation improved plant performance and antioxidant capacity and reduced V accumulation in aboveground tissues. At maturity, these beneficial effects were accompanied by a redistribution of rhizosphere V toward the reducible fraction. Microbial community analysis showed that strain ST-1 did not simply restore the rhizosphere to an uncontaminated state, but selectively restructured key indigenous microbial groups. This microbial restructuring was associated with a recalibration of the rhizosphere metabolic network, particularly involving carbohydrate metabolism, aromatic and nitrogen-containing metabolism, nucleotide metabolism, membrane lipid metabolism, and cofactor metabolism. MAG analysis further identified key genomic carriers harboring genes related to complex carbon mobilization, reverse TCA-associated carbon skeleton conservation, aminotransferase-mediated transformation of nitrogen-containing intermediates, and nar–nir–nor-linked nitrate/nitrite reduction, providing a potential genomic basis for carbon–nitrogen reorganization and local redox regulation. Collectively, these results support an integrated model in which strain ST-1 inoculation promotes selective rhizosphere microbial restructuring and metabolic reprogramming, which are associated with V fraction redistribution and ultimately contribute to reduced V stress and improved maize adaptation. These findings provide multi-omics evidence for regulating V behavior and restoring rhizosphere function in V-contaminated agricultural soils.2026-06-212026-06-21MTBLS14800