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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/m_MTBLS14814_LC-MS_negative_normal-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/m_MTBLS14814_LC-MS_positive_normal-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/a_MTBLS14814_LC-MS_positive_normal-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/a_MTBLS14814_LC-MS_negative_normal-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/s_MTBLS14814.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814/i_Investigation.txtprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14814Lipid identification was performed by matching accurate precursor m/z and MS2 fragmentation spectra against publicly available databases including LipidMaps (https://www.lipidmaps.org/), HMDB (https://hmdb.ca/), and Metlin (https://metlin.scripps.edu/). Identification confidence levels are reported according to the Metabolomics Standards Initiative (MSI): Level 1 (identified by authentic standard or MS2 matching to database with high confidence), Level 2 (putatively annotated based on MS2 matching).MetaboLightsPublicLiquid Chromatography MS - positive - normal-phaseLiquid Chromatography MS - negative - normal-phaseChromatographic separation was performed on a Dionex UltiMate 3000 HPLC system equipped with a ZORBAX RX-SIL normal-phase silica column (250 mm × 4.6 mm, 5 μm, Agilent Technologies). The column temperature was maintained at 35°C. Mobile phase A was hexane, B was isopropanol, and D was water. The flow rate was 1.0 mL/min with a gradient program: 0 min (40% A, 57% B, 3% D), 8 min (40% A, 50% B, 10% D), 15 min (40% A, 50% B, 10% D), 15.1 min (40% A, 57% B, 3% D), 24 min (40% A, 57% B, 3% D), then stop at 32 min. Injection volume was 5 μL. Post-column, the flow was split at a ratio of 1:4, with approximately 200 μL/min entering the ion source.Phosphatidylcholine in wheat anthers is a susceptibility factor for Fusarium head blight.Yazhou ZhangSichuan Agricultural Universityanthermass spectrometry assayLipids were extracted using a modified methanol-chloroform method. Briefly, 100 mg of fresh wheat anther tissue was weighed into a 2 mL tube. 1.5 mL of methanol-chloroform (1:2, v/v) was added, vortexed for 1 min, and centrifuged at 10,000 rpm for 10 min at 4°C. The lower organic phase was transferred to a new tube, and the extraction was repeated twice more. The combined organic layers were washed with 1 mL of 0.75% NaCl solution, vortexed, and allowed to separate. The lower organic phase was collected, dried over anhydrous sodium sulfate, and evaporated to dryness under reduced pressure. The dried extract was reconstituted in 200 μL of hexane-isopropanol (2:3, v/v), vortexed for 1 min, and filtered through a 0.22 μm organic membrane filter prior to LC-MS analysis.Triticum aestivumhttps://www.ebi.ac.uk/metabolights/MTBLS14814Yazhou Zhang. Sichuan Agricultural University. yazhou14716@sicau.edu.cn.Raw data were processed using Thermo Fisher Xcalibur software. The final feature tables (positive and negative modes) containing m/z, retention time, and peak areas were generated. Data were not transformed further.Developmental stageyazhou14716@sicau.edu.cnWheat (Triticum aestivum) anthers were collected at different developmental stages according to the Zadoks scale (Z60, Z65, Z69). Five biological replicates were collected for each stage. Anthers were excised from spikes and immediately frozen in liquid nitrogen, then stored at -80°C until lipid extraction.MetabolomicsProteoWizard msconvertDionex UltiMate 3000 HPLC system (Thermo Fisher Scientific)antherpositive mode adduct ionuntargeted analysisScabheat anther; lipidomics; Fusarium head blight; developmental stage; Zadoks scale; untargeted; LC-MSnegative mode adduct ionThermo Fisher Q Exactive Plus quadrupole-Orbitrap mass spectrometerLipidomicstissueTriticum aestivumProteoWizard msconvertDionex UltiMate 3000 HPLC system (Thermo Fisher Scientific)antherpositive mode adduct ionuntargeted analysisScabheat anther; lipidomics; Fusarium head blight; developmental stage; Zadoks scale; untargeted; LC-MSnegative mode adduct ionThermo Fisher Q Exactive Plus quadrupole-Orbitrap mass spectrometerLipidomicstissueTriticum aestivumMass spectrometry was performed on a Thermo Fisher Q Exactive Plus quadrupole-Orbitrap mass spectrometer equipped with a heated electrospray ionization (HESI) source. Both positive and negative ion modes were acquired separately (two independent assays). Full scan resolution was 70,000 FWHM over a mass range of m/z 100-1500. AGC target was 1000000 with a maximum injection time of 100 ms. Data-dependent MS2 (dd-MS2) was performed at a resolution of 17,500 FWHM with stepped collision energies of 15, 30, and 45 eV, and Top N = 10. Dynamic exclusion time was 8 s. Source parameters: spray voltage 3.5 kV (positive) / 3.0 kV (negative); capillary temperature 320°C; auxiliary gas heater temperature 350°C; sheath gas flow 40 arb; auxiliary gas flow 10 arb.LPC(20:4)TG(16:0/16:0/18:2)DG(20:1/16:4)PC(20:4/18:4)SM(d18:1/20:0)Cer(d18:2/16:0)LPE(16:0)PE(16:3/20:3)PC(16:3/22:0)DG(18:2/18:3)PE(22:3/14:4)PC(18:0/14:3)PC(16:0/18:2)SM(d18:1/16:2)TG(16:0/20:4/20:0)TG(14:0/16:3/20:1)PC(20:0/14:4)PC(14:4/22:0)TG(18:0/20:4/14:2)CE(18:2)PC(16:0/18:3)PC(14:2/18:1)LPC(14:2)TG(20:0/20:3/18:1)PC(22:2/20:1)Cer(d18:1/18:3)PC(18:2/18:3)DG(16:0/18:2)PC(18:1/18:2)SM(d18:1/20:2)DG(14:4/14:2)PE(18:0/18:2)PC(22:3/18:1)TG(16:0/18:2/18:3)Cer(d18:1/16:0)TG(16:1/16:4/18:4)PC(18:4/20:2)LPC(16:3)TG(14:3/18:4/20:4)PC(18:2/18:2)PE(18:1/18:2)LPC(22:1)TG(16:0/20:1/18:3)PC(20:4/16:3)LPE(18:1)PC(16:0/18:1)LPC(14:0)PC(20:2/18:0)LPC(16:2)TG(18:2/18:2/18:3)TG(18:3/16:1/18:0)PE(16:3/16:1)LPC(18:4)LPC(20:0)PE(14:2/18:2)TG(16:1/20:0/22:4)PC(22:1/20:1)DG(22:4/14:2)PE(16:0/18:3)SM(d18:1/18:0)PC(14:2/22:3)PC(14:3/22:0)LPE(20:2)Cer(d18:1/18:0)LPC(18:3)PE(22:2/20:4)LPC(20:1)SM(d18:1/24:1)PC(18:3/16:1)DG(16:2/16:1)LPC(22:3)CE(16:4)PC(20:2/20:2)DG(14:4/18:2)DG(22:2/22:3)PE(16:0/18:2)TG(20:3/20:2/16:3)TG(14:0/18:4/16:1)LPC(16:0)LPC(18:2)LPC(20:2)PC(14:4/16:3)SM(d18:1/22:0)PE(16:1/18:3)LPE(18:4)PE(20:4/14:3)Cer(d18:1/22:1)PE(14:3/16:3)PC(20:0/14:1)TG(22:0/18:1/20:2)SM(d18:1/16:0)PE(18:3/16:3)TG(16:0/18:1/18:2)LPC(18:1)LPC(20:3)CE(18:0)PE(14:1/14:3)PC(18:3/20:0)PE(14:0/14:4)CE(20:4)falsePhosphatidylcholine in wheat anthers is a susceptibility factor for Fusarium head blightFusarium head blight is a devastating wheat disease that initiates at flowering, with anthers as the preferred infection site. However, the anther-derived molecules driving this susceptibility remain elusive. Here, we identify anther-enriched phosphatidylcholine, particularly PC(18:2/18:2) and PC(16:0/18:2), as a key susceptibility factor that promotes Fusarium radial growth, while exogenously applied free linoleic acid specifically enhances conidial germination. Whereas endogenous linoleic acid is associated with defense rather than direct growth promotion.2026-06-232026-06-22MTBLS14814