The extracted dataset (peak table) underwent metabolite structural identification by matching MS/MS spectra against public and in-house libraries (details not specified in the original protocol). Subsequently, data pre-processing was performed: features with missing values >50% were removed; remaining missing values were imputed using K-nearest neighbor (KNN) filling; and features with a relative standard deviation (RSD) >50% in QC samples were filtered out. After these steps, data quality was evaluated using six quality control metrics (as per the in-house pipeline), and the final clean dataset was used for multivariate and univariate statistical analyses.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - hilic","Liquid Chromatography MS - negative - hilic"],"chromatography_protocol":["chromatographic separation was performed on an Agilent 1290 Infinity LC system equipped with a Waters ACQUITY UPLC BEH Amide column (1.7 μm, 2.1 mm × 100 mm) – a HILIC (hydrophilic interaction liquid chromatography) column. The column temperature was maintained at 25°C, the flow rate at 0.5 mL/min, and the injection volume was 2 μL. Mobile phase A consisted of water containing 25 mM ammonium acetate and 25 mM ammonia; mobile phase B was acetonitrile. The gradient elution program was as follows: 0–0.5 min, 95% B; 0.5–7 min, B linearly decreased from 95% to 65%; 7–8 min, B linearly decreased from 65% to 40%; 8–9 min, B held at 40%; 9–9.1 min, B linearly increased from 40% to 95%; 9.1–12 min, B held at 95%. The autosampler temperature was kept at 4°C throughout the analysis. Samples were injected in a randomized order, and quality control (QC) samples were interspersed to monitor system stability and data reliability.
"],"publication":["Therapeutic Effect of Modified Meridian-Guided Acupoint Pressing on Lumbar Facet Joint Osteoarthritis: An Integrated Microbiomics and Metabolomics Analysis."],"submitter_affiliation":["The 923rd Hospital of Joint Logistics Support Force of Chinese People's Liberation Army"],"submitter_name":["Gejin Wei"],"organism_part":["blood serum"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["An appropriate amount of each sample was mixed with a pre-chilled methanol/acetonitrile/water solution (2:2:1, v/v/v). The mixture was vortexed, sonicated at low temperature for 30 min, and then kept at -20°C for 10 min. After centrifugation at 14,000×g at 4°C for 20 min, the supernatant was transferred and dried under vacuum. The dried residue was reconstituted in 100 μL of acetonitrile/water (1:1, v/v), vortexed, and centrifuged at 14,000×g at 4°C for 15 min. The final supernatant was used for LC-MS analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14816"],"author":["Gejin Wei. The 923rd Hospital of Joint Logistics Support Force of Chinese People's Liberation Army. 15240657357@163.com."],"data_transformation_protocol":["Raw data files (.wiff) were converted to the .mzXML format using ProteoWizard. The converted files were then processed with XCMS software for peak alignment, retention time correction, and peak area extraction.
"],"study_factor":["Treatment"],"submitter_email":["15240657357@163.com"],"sample_collection_protocol":["Biological samples were collected and immediately snap-frozen in liquid nitrogen, then stored at -80°C until analysis. Prior to extraction, samples were slowly thawed at 4°C.
"],"omics_type":["Metabolomics"],"study_design":["ProteoWizard msconvert","Metabolomics","Mus musculus","untargeted analysis","Agilent software","blood serum","MzWiff","Agilent 1290 Infinity LC","AB SCIEX TripleTOF 6600","experimental sample"],"curator_keywords":["ProteoWizard msconvert","Metabolomics","Mus musculus","untargeted analysis","Agilent software","blood serum","MzWiff","Agilent 1290 Infinity LC","AB SCIEX TripleTOF 6600","experimental sample"],"mass_spectrometry_protocol":["Data were acquired in both positive and negative electrospray ionization (ESI) modes. Source parameters: Gas1 (nebulizer) 60, Gas2 (heater) 60, curtain gas 30 psi, ion source temperature 600°C, and ion spray voltage ±5500 V. MS1 scan range: m/z 60-1000; MS2 scan range: m/z 25-1000. MS1 accumulation time: 0.20 s/spectrum; MS2 accumulation time: 0.05 s/spectrum. MS/MS spectra were obtained using information-dependent acquisition (IDA) with peak intensity filtering. Declustering potential: ±60 V; collision energy: 35 ± 15 eV. Dynamic exclusion of isotope ions within a 4 Da window was applied, and 10 fragment spectra were collected per cycle.
"],"additional_accession":[]},"is_claimable":false,"name":"Therapeutic Effect of Modified Meridian-Guided Acupoint Pressing on Lumbar Facet Joint Osteoarthritis: An Integrated Microbiomics and Metabolomics Analysis","description":"Lumbar facet joint osteoarthritis (LFJ OA) is a leading cause of chronic low back pain, with limited effective long-term treatments due to the side effects of pharmacological interventions. Modified Meridian-Guided Acupoint Pressing (MMGAP), a Traditional Chinese Medicine (TCM) physical therapy, has shown potential in musculoskeletal pain management, but its therapeutic efficacy and mechanisms in LFJ OA remain unclear. This study aimed to investigate the effects of MMGAP on LFJ OA and explore the underlying mechanisms using integrated microbiomics and metabolomics analyses. Our results showed that MMGAP significantly alleviated histopathological lesions and inflammatory cell infiltration in lumbar muscles and facet joints, reduced serum levels of IL-1β and TNF-α, and downregulated TRPV1 expression in LFJ OA rats. MMGAP also reshaped gut microbiota composition (altered α/β-diversity and differential taxa) and modulated serum metabolomic profiles, with enrichment of the mTOR signaling pathway. Fecal microbiota transplantation (FMT) from MMGAP-treated rats recapitulated the therapeutic effects, confirming gut microbiota as a key mediator. Furthermore, the mTORC1 inhibitor rapamycin mimicked MMGAP’s anti-inflammatory and analgesic effects. Collectively, our findings demonstrate that MMGAP alleviates LFJ OA through a novel mechanism involving gut microbiota reshaping, serum metabolomic reprogramming, and mTORC1 pathway inhibition. This study provides a scientific basis for the clinical application of MMGAP in LFJ OA and highlights the potential of TCM physical therapies as targeted non-invasive alternatives for degenerative joint diseases","dates":{"publication":"2026-06-22","submission":"2026-06-22"},"accession":"MTBLS14816","cross_references":{}}