MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/m_MTBLS14847_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/m_MTBLS14847_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/a_MTBLS14847_LC-MS_positive_reverse-phase-2.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/a_MTBLS14847_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/a_MTBLS14847_LC-MS_positive_reverse-phase-1.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847/s_MTBLS14847.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14847Data analysisAfter normalizing the original peak area information with the total peak area, the follow-up analysis was performed. Principal component analysis and Spearman correlation analysis were used to judge the repeatability of the samples within group and the quanlity control samples. The identified compounds are searched for classification and pathway information in KEGG, HMDB and lipidmaps databases.According to the grouping information, calculate and compare the difference multiples, T test was used to calculate the difference significance pvalue of each compound. The R language package ropls was used to perform OPLS-DA modeling, and 200 times permutation tests was performed to verify the reliability of the model. The VIP value of the model was calculated using multiple cross-validation. The method of combining the difference multiple, the P value and the VIP value of the OPLS-DA model was adopted to screen the differential metabolites. The screening criteria are FC>2, P value<0.05 and VIP>1. The difference metabolites of KEGG pathway enrichment significance were calculated using hypergeometric distribution test.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseMetabolites ExtractionThe LC/MS system for metabolomics analysis is composed of Waters Acquity I-Class PLUS ultra-high performance liquid tandem Waters Xevo G2-XS QTof high resolution mass spectrometer. The column used is purchased from Waters Acquity UPLC HSS T3 column (1.8um 2.1*100mm)Positive ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrileNegative ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrileInjection volume 2μLGrowth-Promoting Mechanisms of Synthetic Communities in Tomato and Wheat under Saline-Alkaline Stress.Northwest A&F UniversityQiuting Yanwheat heading stage leafWheat heading stage rhizosphere soilTomato flowering stage rhizosphere soilmass spectrometry assaySolid samples(1) A total of 50 mg of each solid sample was weighed, followed by the addition of 1000 μL extraction solution supplemented with internal standards (methanol:acetonitrile:water = 2:2:1, v/v/v; the concentration of internal standards was set at 20 mg/L). The mixture was vortexed thoroughly for 30 s.(2) Steel beads were added to the mixture, and homogenization was performed using a tissue grinder at 45 Hz for 10 min. Subsequent sonication was carried out for 10 min in an ice-water bath.Solanum lycopersicumTriticum aestivumhttps://www.ebi.ac.uk/metabolights/MTBLS14847Qiuting Yan. Northwest A&F University. 1979980928@qq.com.Wenxaing He. Northwest A&F University. wenxiang.he@nwafu.edu.cn.Data preprocessing and annotationThe raw data collected using MassLynx V4.2 is processed by Progenesis QI software for peak extraction, peak alignment and other data processing operations, based on the Progenesis QI software online METLIN database and Biomark’s self-built library for identification.Group1979980928@qq.comRhizosphere soil samples were collected at the flowering stage of tomato and the heading stage of wheat. Plants with uniform growth status were randomly selected for sampling, and tightly adherent rhizosphere soils were gently stripped under sterile conditions. All collected samples were immediately frozen in liquid nitrogen and subsequently stored at 80 °C.MetabolomicsMetabolomicswheat heading stage leafACQUITY UPLC I-Class PLUStargeted analysisuntargeted analysisSolanum lycopersicumSalt tolerance-growth promotionExtractTomatoAB SCIEX QTRAP 6500+Waters Xevo G2-XS QTofWheat heading stage rhizosphere soilTomato flowering stage rhizosphere soilPGPRWheatTriticum aestivumMetabolomicsACQUITY UPLC I-Class PLUSwheat heading stage leaftargeted analysisuntargeted analysisSolanum lycopersicumSalt tolerance-growth promotionExtractTomatoAB SCIEX QTRAP 6500+Waters Xevo G2-XS QTofWheat heading stage rhizosphere soilTomato flowering stage rhizosphere soilPGPRWheatTriticum aestivumLC-MS/MS AnalysisWaters Xevo G2-XS QTOF high resolution mass spectrometer can collect primary and secondary mass spectrometry data in MSe mode under the control of the acquisition software (MassLynx V4.2, Waters). In each data acquisition cycle, dual-channel data acquisition can be performed on both low collision energy and high collision energy at the same time. The low collision energy is off, the high collision energy range is 10~40V, and the scanning frequency is 0.2 seconds for a mass spectrum. The parameters of the ESI ion source are as follows: Capillary voltage: 2500V (positive ion mode) or -2000V (negative ion mode); cone voltage: 30V; ion source temperature: 100°C; desolvent gas temperature 500°C; backflush gas flow rate: 50L/ h; Desolventizing gas flow rate: 800L/h.falseGrowth-Promoting Mechanisms of Synthetic Communities in Tomato and Wheat under Saline-Alkaline StressSynComs boosted saline-alkali resistance in tomato and wheat via host-specific reshaping of rhizosphere microhabitats and distinct core plant metabolic pathways.2026-06-242026-06-24MTBLS14847