MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14856/m_MTBLS14856_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14856/a_MTBLS14856_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14856/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14856/s_MTBLS14856.txtprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14856Compound identification was performed by searching against the public databases (METLIN and HMDB) and an in-house database (BestMS Technologies, Qingdao, China).MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phaseThe LC-MS/MS analyses were performed on a UHPLC system (ACQUITY UPLC I-Class PLUS, Waters) coupled with a QTOF mass spectrometer (Xevo G2-XS, Waters). Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm) at a flow rate of 0.4 mL/min. The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). The gradient program was as follows: 0-0.5 min, 5% B; 0.5-5.0 min, 5-50% B; 5.0-7.5 min, 50-95% B; 7.5-8.5 min, 95% B; 8.5-10.0 min, 5% B.Pithecellobium clypearia ameliorates ulcerative colitis through regulation of purine metabolism and gut microbiota.Huazhong University of Science and Technologypan yancolonmass spectrometry assayColon samples (50 mg) were mixed with 1000 μL of extraction solvent (methanol: acetonitrile: water = 2:2:1, v/v/v) containing 20 mg/L of internal standard, and vortexed for 30 s. Subsequently, the mixture was homogenized at 45 Hz for 10 min and sonicated in an ice-water bath for 10 min. The mixture was incubated at -20 °C for 1 h, followed by centrifugation at 12,000 rpm for 15 min at 4 °C. A 500 μL aliquot of the supernatant was dried using a vacuum concentrator. The dried residue was then reconstituted in 160 μL of reconstitution solvent (acetonitrile: water = 1:1, v/v). After vortexing for 30 s and sonication in an ice-water bath for 10 min, the sample was centrifuged again at 12,000 rpm for 15 min at 4 °C. Finally, the resulting supernatant was used for LC-MS/MS analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14856pan yan. Central Hospital of Changsha. panyan_science@163.com.The raw data were processed using Progenesis QI software for peak picking, alignment, and other data preprocessing operations.Dosepanyan_science@163.comColon was rinsed with ice saline, and the part of tissue was fixed in 4% paraformaldehyde solution for histopathological examination, another part of colon was detected tight junction and untargeted metabolomics.MetabolomicsMetabolomicsMus musculusWaters ACQUITY UPLC I-Class PLUS SystemWaters Xevo G2-XS QTofuntargeted analysiscolonulcerative colitisexperimental blankProgenesis QINontargeted metabolomicsMetabolomicsMus musculusWaters ACQUITY UPLC I-Class PLUS SystemWaters Xevo G2-XS QTofuntargeted analysiscolonulcerative colitisexperimental blankProgenesis QINontargeted metabolomicsThe MS conditions were as follows: capillary voltage, 2500 V (positive) or -2000 V (negative); cone voltage, 30 V; ion source temperature, 100 °C; desolvent gas temperature, 500 °C; backflush gas flow rate, 50 L/ h; desolventizing gas flow rate, 800 L/h.falsePithecellobium clypearia ameliorates ulcerative colitis through regulation of purine metabolism and gut microbiotaBackground: Pithecellobium clypearia (PC) is a traditional Chinese medicine with anti-inflammatory effects. However, the therapeutic efficacy and underlying mechanisms of PC in ulcerative colitis (UC) remain unclear.Objective: This study applied an integrated microbiome-metabolomics approach to investigate PC’s pharmacological mechanisms against UC.Materials and methods: A dextran sulfate sodium (DSS)-induced UC model was established, and the therapeutic efficacy of PC was evaluated using the disease activity index (DAI), histopathology, inflammatory cytokines, and tight junction proteins. Subsequently, 16S rRNA sequencing and metabolomics were performed to analyze alterations in the gut microbiota composition and colonic metabolic profiles.Results: PC significantly ameliorated DSS-induced weight loss, reduced DAI scores, prevented colon shortening, and alleviated mucosal damage. Furthermore, PC decreased pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), increased anti-inflammatory IL-10, and restored ZO-1 and occludin expression. 16S rRNA sequencing showed PC restored gut dysbiosis, enriching beneficial Bacteroides while reducing pathogenic Escherichia-Shigella. Metabolomics indicated PC modulated colonic purine metabolism, normalizing key metabolites like xanthylic acid and aminoimidazole ribotide. Correlation analysis confirmed a strong crosstalk between these purine metabolites and specific gut microbiota.Discussion and conclusions: PC ameliorates UC by suppressing inflammation and protecting the intestinal barrier. Its therapeutic mechanisms may be associated with the regulation of gut microbiota and host purine metabolism, providing a theoretical basis for future clinical applications.2026-06-262026-06-25MTBLS14856