Metabolite feature identification and qualitative matching were performed using an in-house R package. Metabolite annotation and identification were achieved by comparison against the BiotreeDB database (V3.0).
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - reverse-phase"],"chromatography_protocol":["Chromatographic separation was performed using a Waters ACQUITY UPLC BEH Amide column (2.1 mm × 50 mm, 1.7 μm). Mobile phase A consisted of 25 mmol/L ammonium acetate and 25 mmol/L ammonia in ultrapure water, and mobile phase B was acetonitrile. Gradient elution was performed as follows: 0–0.25 min, 95% B; 0.25–3.5 min, 95%–65% B; 3.5–4.0 min, 65%–40% B; 4.0–4.5 min, 40% B; 4.5–4.55 min, 40%–95% B; and 4.55–6.0 min, 95% B. The column temperature was maintained at 25°C, the autosampler temperature was set at 4°C, the flow rate was 0.5 mL/min, and the injection volume was 2 μL.
"],"publication":["Terminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota."],"submitter_affiliation":["Peking University"],"submitter_name":["Duo Keai"],"organism_part":["feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["A 25 mg sample was weighed under low-temperature conditions and transferred into an EP tube. Two homogenization beads were added, followed by 500 μL of extraction solvent consisting of methanol, acetonitrile, and water at a ratio of 2:2:1 (v/v/v). The extraction solvent contained isotope-labeled internal standards. The mixture was vortexed for 30 s and then homogenized using a tissue homogenizer at 35 Hz for 4 min. Subsequently, the sample was subjected to ultrasonic extraction in an ice-water bath for 5 min. This procedure was repeated three times. The sample was then incubated at -40°C for 1 h, followed by centrifugation at 12,000 rpm at 4°C for 15 min, corresponding to a centrifugal force of 13,800 × g with a rotor radius of 8.6 cm. The supernatant was collected and transferred into an injection vial for instrumental analysis.
"],"organism":["Rattus norvegicus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14867"],"author":["Song Lin Song. Inner Mongolia Medical University. songlinwps@163.com.","Sarina SA. Inner Mongolia Medical University. 1969135715@qq.com."],"data_transformation_protocol":["Raw metabolomics data were converted and preprocessed using a standard metabolomics data-processing workflow. First, outlier filtering was performed based on relative standard deviation/coefficient of variation (RSD/CV) to remove noise. Second, missing-value filtering was conducted, and only peak-area data with no more than 50% missing values within a single group and no more than 50% missing values overall were retained. Third, missing values were imputed using one-half of the minimum value. Fourth, data normalization and standardization were performed based on internal standards (IS). After conversion and preprocessing, 33,012 features were retained from the original 46,630 peaks for subsequent analysis.
"],"study_factor":["Group"],"submitter_email":["keaiduoduo998@126.com"],"sample_collection_protocol":["Fresh fecal samples were collected from experimental Sprague-Dawley (SD) rats on November 10, 2024. The samples were immediately collected under aseptic conditions, with visible impurities removed, and rapidly aliquoted into sterile cryopreservation tubes. After collection, the samples were immediately stored in a -80°C ultra-low-temperature freezer for long-term preservation until subsequent metabolomic analysis.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Vanquish UHPLC System","Metabolomics","A. kusnezoffii","untargeted analysis","Thermo Scientific Orbitrap Exploris 120","Rattus norvegicus","Terminalia chebula decoction processing; metabolomics","feces","intestinal flora","PI3K/Akt","pharmacodynamic effect"],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","Metabolomics","A. kusnezoffii","untargeted analysis","Thermo Scientific Orbitrap Exploris 120","Rattus norvegicus","Terminalia chebula decoction processing; metabolomics","feces","intestinal flora","PI3K/Akt","pharmacodynamic effect"],"mass_spectrometry_protocol":["Mass spectrometric analysis was performed using an Orbitrap Exploris 120 MS system equipped with an electrospray ionization (ESI) source. Data were acquired in both positive and negative ion modes. The sheath gas flow rate was set to 50 arb, and the auxiliary gas flow rate was set to 15 arb. The capillary temperature was maintained at 320°C. The MS resolution was 60,000, and the MS/MS resolution was 15,000.
"],"metabolite_name":["7-hydroxy-2-methyl-3-phenyl-4H-chromen-4-one","3-Hydroxy-b,e-caroten-3'-one","Caprolactam","Malonyl-L-carnitine","Mangiferdesmethylursanone","6alpha-methylprednisolone","Murrayamine_A","N1,N10-Dicoumaroylspermidine","4-Carboxyphenylglycine","PC(18:0/15:0)","Ectoine","Val-Met","Methyl_6-O-galloyl-beta-D-glucopyranoside","LysoPE(0:0/18:1(9Z))","Norphenylephrine","4-Methoxy-3-geranylgeranyl-1,2-dihydroxybenzene","2-Keto-6-acetamidocaproate","Reserpine","Normorphine","Cholest-4-en-3-one","4-Hexylresorcinol","1-Butanone, 1-(1,3-benzodioxol-5-yl)-2-(dimethylamino)-","N-Methylcalystegine_C1","Nipecotic acid","CE(5:0)","1-(1Z-Octadecenyl)-sn-glycero-3-phosphocholine","Glutarylglycine","4-Oxo-1,4-dihydro-3-quinolinecarboxylic acid","beta-Citraurol","(4R)-4-((1R,3S,5S,7R,9S,10S,12S,13R,14S,17R)-1,3,7,12-tetrahydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoic acid","N-Ethylglycine","Hyocholic acid","Meglumine","D-Glucosaminic acid","LysoPC(24:1(15Z))","3-(Methylthio)-1-propene","DG(16:1(9Z)/15:0/0:0)","Picolinamide","N-Methylcalystegine_B2","PE(20:3(8Z,11Z,14Z)/22:0)","2-Hydroxy-3-(3,4-dihydroxyphenyl)propanamide","Pilocarpine","PE(20:1(11Z)/14:1(9Z))","Zanthobisquinolone","3H-Pyrazol-3-one, 1,2-dihydro-2-[6-(4-morpholinyl)-4-pyrimidinyl]-4-(1H-1,2,3-triazol-1-yl)-","Gamma-glutamyl-Glutamine","Bisphenol A diglycidyl ether","Linalool_3,7-oxide_beta-primeveroside","Secobarbital","PC(16:0/18:0)","DG(18:2(9Z,12Z)/16:1(9Z)/0:0)","Propionylpromazine (hydrochloride)","AMP-Deoxynojirimycin","DG(18:3(9Z,12Z,15Z)/15:0/0:0)","PC(P-16:0/18:2(9Z,12Z))","Ergostane-3,6-dione","2-Hydroxy-6-tridecylbenzoic acid","Curcumin_III","Thaumatin_b,_recombinant","Withanolide","(2beta,3alpha,9alpha,24R)-Ergosta-7,22-diene-2,3,9-triol","LacCer(d18:1/16:0)","N6-Methyladenine","2-Propylthiazolo[4,5-c]quinolin-4-amine","Kainic acid","Prednisolone_tebutate","Gln-Pro","Hygric acid","Auxin_a","Phenylalanine betaine","Ursocholic acid","1-O-Hexadecyl-sn-glycero-3-phosphocholine (LPC(O-16:0/0:0))","7-Ketocholesterol","Xanthurenic acid","PE(24:1(15Z)/P-16:0)","Saquinavir","Benzocaine","1-(1Z-Octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine","Bicifadine","3-Nitrophenylhydrazine","Metalaxyl","STIGMASTA-4,22-DIEN-3-ONE","Brucine","cyclopentolate","25-Acetylvulgaroside","1-Docosahexaenoyl-2-stearoyl-sn-glycero-3-phosphocholine","5-Methoxytryptamine","Mupirocin","Prolylalanine","Hexadecadienoylcarnitine (Car(16:2))","4beta-Hydroxycholesterol","Metoprolol acid","Gly-Ile","L-Glycine ether ester","Desethyleneciprofloxacin","Methylguanidine","PE(22:4(7Z,10Z,13Z,16Z)/14:0)","cis-7,10,13,16-Docosatetraenoic acid","N-Oleoyltaurine","3-Methyladenine","MLS000859943-01!","(8'R)-Neochrome","4-Methyl-5-vinylthiazole","Pisumoside_B","8-Shogaol","LPE(P-16:0)","(3beta,5alpha,6beta,22E,24R)-23-Methylergosta-7,22-diene-3,5,6-triol","Promethazine sulfoxide","N,N-Diethyl-2-aminoethanol","Icaridin","(3beta,17alpha,23S)-17,23-Epoxy-3,29-dihydroxy-27-norlanost-8-en-24-one","Masitinib (AB1010)","Adenosine","3,6,9,12-Tetraoxatetracosan-1-ol","L-tyrosine-methyl-ester","Oxyphenonium","PC(18:0/18:1(9Z))","PA(20:4(5Z,8Z,11Z,14Z)/22:4(7Z,10Z,13Z,16Z))","(3S,3'R,4xi)-beta,beta-Carotene-3,3',4-triol","Desglucocoroloside","Helinorbisabone","5-Methylcytosine","PC(15:0/P-18:0)","Suillin","Fenpropathrin","1h-indole-3-butanoic acid","Phenformin","Dalfopristin","Nigellimine_N-oxide","1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine","Sphingosine","(S)-(-)-Perillyl_alcohol","3-Oxostearic acid","Mesaconine","METARAMINOL","Threonine","(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl ditetradecanoate","Lys-Leu","Diferuloylputrescine","N-Acetyl-arginine","N-(3-PHENYLPROPIONYL)GLYCINE","Alanyl-Leucine","Paracetamol (Drug)","Phenylethylmalonamide","Linoleoylcarnitine (Car(18:2))","Heptaminol","4-Amino-2-oxo-1,2-dihydropyrimidine-5-carboxylic acid","Cabergoline","3-Aminohexanoic acid","1,2-Dieicosenoyl-sn-glycero-3-phosphocholine","Naphtho[2,3-b]furan-2(4H)-one, 4a,5,6,7,8,8a,9,9a-octahydro-3,8a-dimethyl-5-methylene-","Thesinine_4'-O-glucoside","Asperagenin","Dulciol_A","MG(22:5(4Z,7Z,10Z,13Z,16Z)/0:0/0:0)","Phloionolic_acid","2-O-Methylcytosine","8,9-epoxy-3-isobutyryloxy-10-(2-methylbutanoyl)thymol","Meperidine","1-Methylnicotinamide","Ethyl 3-(N-butylacetamido)propionate","(3beta,15alpha,22S,24E)-3,15,22-Trihydroxylanosta-7,9(11),24-trien-26-oic_acid","1,25-Dihydroxyvitamin_D3-26,23-lactone","2-(2-Furyl)piperidine","Oryzanol_A","PC(18:4(6Z,9Z,12Z,15Z)/14:0)","1-(2-Thienyl)-1-heptanone","Isoxaben","1-Aminocyclohexanecarboxylic_acid","Syrosingopine","N'-Nitrosonornicotine","7-Methylguanine","Quercetin_3-(2-caffeoylglucuronoside)","Glycylserylprolylmethionylphenylalanylvalinamide","DG(18:1(11Z)/20:3(5Z,8Z,11Z)/0:0)","(3R,5S,7R,9S,10S,12S,13R,14S,17R)-17-((2R)-7-hydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol","Quinestrol","Mepirizole","4-Hydroxyvalsartan","LysoPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z))","Pipecolic acid","papaverine","Prolyl-Serine","2-Amino-3-methoxybenzoic acid","Testosterone_phenylpropionate","1-Methylpiperazine","Lys-Pro","DG(20:5(5Z,8Z,11Z,14Z,17Z)/20:0/0:0)","Ala-Pro","2-Ethylpyrazine","PE(14:0/15:0)","7H-Purine-7-acetamide, 1,2,3,6-tetrahydro-1,3-dimethyl-N-[4-(1-methylethyl)phenyl]-2,6-dioxo-","Carbetapentane","Mometasone_furoate","2,4-Dimethoxyaniline","3alpha-Hydroxyoreadone","Carnitine","Prostaglandin_PGE2_1-glyceryl_ester","PC(14:0/P-16:0)","Anhydroecgonine methyl ester","LPC(16:0)","2-Amino-3,4,8-trimethyl-3H-imidazo[4,5-f]quinoxaline","Lenalidomide__","(22E,24R)-Stigmasta-4,22-diene-3,6-dione","Cilazapril","Diisooctyl phthalate","Alanylalanine (Ala-Ala)","Acetildenafil","PC(20:4(8Z,11Z,14Z,17Z)/15:0)","1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine","N6,N6,N6-Trimethyllysine","Macrophorin_A","2,4,6-Trimethylpyridine","8-Desoxygartanin","Gramine","Stercobilin","glycyl-proline","Trifluoroperazine","TOBRAMYCIN","3-Methylhistamine","schisandrin B","PC(18:1(9Z)/P-18:0)","2-Propene-1-thiol","apo-[3-methylcrotonoyl-CoA:carbon-dioxide_ligase_(ADP-forming)]","1-Methylhistamine","Valenciachrome","Car(18:1)","Adlupone","PE(16:0/15:0)","Androsterone_sulfate","Camellenodiol","Trimethobenzamide","DG(22:2(13Z,16Z)/20:3(5Z,8Z,11Z)/0:0)","(25R)-3alpha,7alpha,12alpha,24S-tetrahydroxy-5beta-cholestan-26-oic_acid","alpha-Amyrone","Sph(d18:0)","Trigonelline","(.+/-.)-Dropropizine","3-Hydroxy-carbofuran","(3beta,5alpha,6beta,9alpha,22E,24R)-23-Methylergosta-7,22-diene-3,5,6,9-tetrol","ursolic acid","Pentoxifylline","Cyclohexaneacetic_acid","Isoleucylvaline","Ponatinib_(AP24534)","(E)-Avenanthramide_D","6-methyltetrahydropterin","N-nornuciferine","DG(24:0/18:4(6Z,9Z,12Z,15Z)/0:0)","Fenfluramine","2,5-Dimethylpyrazine","Tangeraxanthin","Val-Ile","Hordatine_A","4-(Dimethylamino)pyridine","(S)-Spinacine","Momorcharaside_B","CAPSAICIN","m-Hydroxybenzoylecgonine","CYCLOPENTANONE","Mofebutazone","3-Methyl-.gamma.-butyrolactone","3-Hydroxylidocaine","Prolylhydroxyproline","Plantaricin_BN","Butalbital","Gitogenin","Xanthoxylin","Myosmine","2'-Deoxyinosine-5'-triphosphate trisodium salt","Tryptamine","2-hydroxyphytanic_acid","Hexobarbitone","Temsirolimus","2-Methylpropanamine","7-ethenyl-1,4a,7-trimethyl-3,4,6,8,8a,9,10,10a-octahydro-2H-phenanthrene-1-carboxylic acid","Promazine","Creatine","Minoxidil","(4OH,8Z,t18:1)_sphingosine","3-hydroxy-sebacic_acid","NAE(18:2)","Palmitoleylcarnitine (Car(16:1))","2,3,4,5-Tetrahydro-6-(5-methyl-2-furanyl)pyridine","Schleicherastatin_3","Octinoxate","Dihydrorhodamine 123","3-Succinoylpyridine","Tolazoline (hydrochloride)","Bisoprolol","7,8-Dihydroxyflavone","Maltoxazine","N2-(1-Oxo-4-phenylbutyl)-L-glutamine","2-Hydroxyenterodiol","Benzoylaconine","2-Imino-1-imidazolidineacetic acid","Carvedilol","N,N-Dimethylarginine (ADMA)","Cyclo(glycylprolyl)","Pehanorm","Arachidyl_carnitine","PE(P-36:4)","Rubraflavone_C","Desmosterol","Permetin_A","Val-Val","LPC(18:0/0:0)","N-Acetyl-D-glucosaminyldiphosphodolichol","Ethephon","Rhizonin_A","Ceritinib","Piperanine","Harmaline","2,5-Dimethyl-3-(1-propenyl)pyrazine","Spirotaccagenin","CDP-DG(18:0/22:3(10Z,13Z,16Z))","Falimint","1-Hydroxy-3,6,7-trimethoxy-2,8-diprenylxanthone","5-[[17-(5,6-dihydroxy-6-methylheptan-2-yl)-3,12-dihydroxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-2-yl]oxy]-3-hydroxy-3-methyl-5-oxopentanoic acid","Amobarbital","15-formyl-16-hydroxy-1,2,14,17,17-pentamethyl-8-(prop-1-en-2-yl)pentacyclo[11.7.0.0,.0,.0,]icosane-5-carboxylic acid","1-Deoxy-D-glucitol","Norepinephrine","DHA ethyl ester","Quassimarin","1-O-trans-cinnamoyl-beta-D-glucopyranose","Garcimangosone_A","PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)","Dimethylethanolamine","1-Hexadecyl lysophosphatidic acid","12,13-Epoxy-9,15-octadecadienoic_acid","Piperazine, 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)-","1,3-Bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea","Histidine","Tetranorprostaglandin J2","Camelledionol","Ascaridole","PE(22:2(13Z,16Z)/0:0)","Tyramine","PC(14:0/15:0)","Quinaprilat","Palmitoylcarnitine (Car(16:0))","Arg-Pro-Pro","9E,11E-Octadecadienoic acid","2-Hexenoylcholine","1-alpha-Acevaltrate","Deoxyadenosine","Melezitose","PE(22:1(13Z)/P-16:0)","2-Fluoro-7H-purin-6-amine","Amikacin","Ala-Ser","Pulcherosine","LysoPE(0:0/22:0)","Cholesta-4,6-dien-3-one","Trilostane","Physalin_L","Sulpiride","Annocherin_A","Thr-Leu","Cyanidin-3-O-sambubioside-5-O-glucoside","Hydrocortamate","1,9-Nonanedithiol","Ganodosterone","MLS001304073-01!Prednisone53-03-2","3-Pyridylacetic acid","2-Deoxysepiapterin","Frangulanine","Glaucarubin","Butyl_oleate_sulfate","Dodecanoic acid, 12-[[(tricyclo[3.3.1.13,7]dec-1-ylamino)carbonyl]amino]-","1-Isothiocyanato-4-phenylbutane","Tyrosyl-Tyrosine","N-Acetylputrescine","Tryptophan","Fluvoxamine_acid","DG(20:3(5Z,8Z,11Z)/20:4(8Z,11Z,14Z,17Z)/0:0)","Glycyrrhetinate","PC(16:0/20:4)","Choline","1,2-Dioleoyl-rac-glycerol","(3b,6b,8a,12a)-8,12-Epoxy-7(11)-eremophilene-6,8,12-trimethoxy-3-ol","LysoPC(14:1(9Z))","Tanshinone Iia","Trandolaprilat","(8R,8'R)-Secoisolariciresinol_9-glucoside","PE(20:4(5Z,8Z,11Z,14Z)/0:0)","3-[Hexadecyl(dimethyl)ammonio]-1-propanesulfonate","2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphoserine","1-isothiocyanato-8-methanesulfinyloctane","1-Aminopropan-2-ol","Ilicifolinoside_A","Soyasaponin_III","Aniline","(9S,10S)-9,10-dihydroxyoctadecanoic_acid","Adenosine monophosphate (AMP)","12'-Apozeaxanthin","Cer(d18:1/18:1(11Z))","Dulcin","o-Tyrosine","Hydrocodone","1,2-Di(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine","Neryl_glucoside","Phosphorylcholine","Idoxanthin","Cepagenin","(-)-trans-4-[4-(4'-Fluorophenyl)-3-piperidinylmethoxy]-2-methoxyphenol","HMMNI","Mildronate","Acetamide, 2-[4-[(5,6-diphenyl-2-pyrazinyl)(1-methylethyl)amino]butoxy]-N-(methylsulfonyl)-","Fenamiphos","LysoPC(22:1(13Z))","Dihydrothymine","Myricolal","Piperidine","Panaxynol","Pro-Pro","Arg-Ala","9S,11R-Dihydroxy-15-oxo-13E-prostenoic acid","Olomoucine","(3beta,22E,24R)-Ergosta-4,6,8(14),22-tetraen-3-ol","Dethiobiotin","Arecaidine","PG(16:1(9Z)/18:3(9Z,12Z,15Z))","Tetramethylquercetin_3-rutinoside","Norbuprenorphine","Cer(d18:1/18:1(9Z))","trans-S-(1-Propenyl)-L-cysteine","1,7-Dimethylxanthine","Methohexital","Thr-Phe","Doxazosin","1-O-Octadecyl-sn-glyceryl-3-phosphorylcholine","N-Jasmonoyltyrosine","Indican","Riboflavin","MeAIB","clobenzorex","Ergocalciferol (Vitamin D2)","Anhydroamarouciaxanthin_B","Urolithin B","Isoleucylleucine","N-Desmethyltramadol","Leucine methyl ester","(9R,10S,12Z)-9,10-Dihydroxy-8-oxo-12-octadecenoic_acid","p-Acetaminobenzaldehyde","PA(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))","(6beta,24R)-6-Hydroxystigmast-4-en-3-one","Camelinin","Pholcodine","Lys-Val","DG(22:5(4Z,7Z,10Z,13Z,16Z)/20:3(5Z,8Z,11Z)/0:0)","DG(22:4(7Z,10Z,13Z,16Z)/22:2(13Z,16Z)/0:0)","Acetic acid, 2-[4-[(5,6-diphenyl-2-pyrazinyl)(1-methylethyl)amino]butoxy]-","(14alpha,17beta,20S,22R)-14,20-Epoxy-17-hydroxy-1-oxowitha-3,5,24-trienolide","Harman","1-Isothiocyanato-7-(methylthio)heptane","5-O-Methylembelin","DG(18:4(6Z,9Z,12Z,15Z)/15:0/0:0)","2-Methyl-4'-(methylthio)-2-morpholinopropiophenone","Biotin","Kukoamine B","Buflomedil","Ligustrazine","Physapubescin","Atrazine desethyl","Armillane","(2E,4E,12Z)-N-(2-methylpropyl)octadeca-2,4,12-trienamide","DHAP(18:0e)","alpha-Tocopherol (Vitamin E)","Cholestan-3-One","PC(16:1(9Z)/0:0)[U]","Homoveratrylamine","4-Hydroxynornantenine","Dinotefuran","Liensinine","etheno-dA","Dicyclomine","2'-O-Methyladenosine","2,6-Dimethylpyrazine","Anastrozole","(3beta,23E)-3-Hydroxy-27-norcycloart-23-en-25-one","Miglitol","DMPO","N-Acetylvanilalanine","MLS001077323-01!51333-22-3","2,3-Butanediol_apiosylglucoside","2-Dimethylamino-6-hydroxypurine","Luminespib","1-O-Hexadecyl-2-O-(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-sn-glyceryl-3-phosphorylcholine","N-Acetylcadaverine","Ifenprodil","PC(18:0/14:0)","DG(22:5(7Z,10Z,13Z,16Z,19Z)/22:2(13Z,16Z)/0:0)","LysoPE(16:1(9Z)/0:0)","Isopentenyl-Adenine","Cer(d18:0/16:0)","Butorphanol","3-Hydroxyhexadecanoylcarnitine","5-Acetylamino-6-amino-3-methyluracil (AAMU)","N-[(4E,8E)-1,3-dihydroxyoctadeca-4,8-dien-2-yl]hexadecanamide","PC(15:0/20:1(11Z))","Bullatine B","LysoPC(18:1(11Z))","2-Methyl-5-HT","N2-(3-Carboxy-2-hydroxy-1-oxopropyl)arginine","Piracetam","Di-4-coumaroylputrescine","1,2-Dipalmitoleoyl-sn-glycero-3-phosphocholine","isepamicin","4,8_dimethylnonanoyl_carnitine","Enterocin_900","trans-Zeatin","PC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:5(4Z,7Z,10Z,13Z,16Z))","PC(P-32:0)","PC(15:0/18:3(9Z,12Z,15Z))","14-(hydroxymethyl)-5,9-dimethyltetracyclo[11.2.1.0,.0,]hexadecane-5-carbaldehyde","Hecogenin","Spiperone","Palmitoyl sphingomyelin","PC(18:0/16:0)","L-Thyronine","1-Methylguanine","Cer(d18:0/14:0)","Pindolol","DG(22:2(13Z,16Z)/20:4(5Z,8Z,11Z,14Z)/0:0)","(3R,7R,9S,10S,12S,13R,14S,17R)-17-((2R)-6,7-dihydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol","1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine","12-Ketoporrigenin","5',5''',8,8''-Tetrahydroxy-3',3''',4',4''',7',7''-hexamethoxy-5,5''-biflavan","Heneicosanoic acid (21:0)","3alpha,4,7,7alpha-Tetrahydro-4-hydroxy-1H-isoindole-1,3(2H)-dione","Phenylalanyl-Glycine","2,4-Diamino-6-nitrotoluene","Galunisertib","Notoginsenoside_J","Stigmasterol","Methiocholic acid putative","Pesticide3_Carbofuran_C12H15NO3_Furadan","Diethanolamine","Cerulenin","Thiamine","Soyasapogenol B base + O-HexA-Hex-Pen, O-C6H7O3(DDMP)","PC(20:2(11Z,14Z)/15:0)","Chlordiazepoxide","5-(12-Heneicosenyl)-1,3-benzenediol","leonurine","3,8-dihydroxy-3,8-dimethyl-5-propan-2-ylidene-1,2,3a,4,7,8a-hexahydroazulen-6-one","3,6-Ditigloyloxytropan-7-ol","Methdilazine","Olaquindox","Phenylalanylphenylalanine","Daltogen","N6-Acetyllysine","N-[2-(4-Prenyloxyphenyl)ethyl]tiglamide","1-[1,4-Dihydro-4-nonyl-5-(1-oxodecyl)-3-pyridinyl]-1-dodecanone","Norverapamil","betulin","Lyciumoside_I","2-Aminonaphthalene","3'-O-Methyladenosine","N-Oleoyl-D-erythro-sphinganine","Dovitinib","Elastin","Spectinomycin","Sterebin_C","Sterebin_A","PE(P-16:0/22:0)","Urocanic acid","1-Hydroxy-3-methyl-9H-carbazole","Cer(d18:0/18:0)","Pirenperone","5-Norbornene-2,3-dicarboximide, N-(2-ethylhexyl)-","DG(20:4(5Z,8Z,11Z,14Z)/18:1(11Z)/0:0)","Piperolactam_D","Neferine","Metanephrine","2,3-Diethylpyrazine","2-Aminopimelate","Nicotinamide adenine dinucleotide (NAD)","Rotundine","2-Amino-5-phenylpyridine","Tetraethylene glycol","3-Pyridinemethanol","5'-Methylthioadenosine","PC(24:1(15Z)/22:2(13Z,16Z))","Cer(d18:1/16:0)","Eremanthin","Acetophenone","1,2-Diarachidonoyl-sn-glycero-3-phosphocholine","Priverogenin_A","2-Hydroxypyridine","Butenafine","Creatinine","Antibiotic_X_14889C","4-Butyloxazole","Quinoline, 4-[6-[4-(1-piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]-","PC(15:0/22:2(13Z,16Z))","Madlongiside_D","Cyproheptadine","Formiminoglutamic acid","7alpha,12alpha,26-trihydroxycholest-4-en-3-one","Stachydrine","7',8'-Dihydro-8'-hydroxycitraniaxanthin","beta-Tocotrienol","Carisoprodol","PG(20:4(5Z,8Z,11Z,14Z)/20:2(11Z,14Z))","Paromomycin","8R-HpODE","Coenzyme_Q4","Theobromine","Bilirubin","[8]-Paradol","Crispolide","2-(10-Heptadecenyl)-6-hydroxybenzoic_acid","Argininic_acid","Betaine","Luminol","N,N-Diethylcathinone","Formylfusarochromanone","Ginsenoyne_A_linoleate","Phenacetin","Cer(d18:1/18:0)","Sarcodon_scabrosus_Depsipeptide","N-Carboxyethyl-r-aminobutyric_acid","Manglupenone","Mesaconitine","1-(1Z-Octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine","5,8-Epoxy-5,8-dihydro-10'-apo-b,y-carotene-3,10'-diol","1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N,N-dimethyl","ABOA","DOCOSANOL","Ethyl 3-aminobenzoate","hexadecanedioic_acid_mono-L-carnitine_ester","1-Stearoyl-2-docosahexaenoyl-sn-glycerol","4-Megastigmene-6a,9R-diol_9-[apiosyl-(1->6)-glucoside]","Candoxatril","Succinamide","Mesobilirubinogen","Goyaglycoside_g","LPE(14:0)","5-Methyl DL-glutamate","PhytoCer(18:0/16:0)","(2alpha,3beta)-2,3-dihydroxyolean-12-en-28-oic acid","Suxibuzone","H-Val-Ala-OH","Aliskiren","Musanolone_F","Dimethylbenzimidazole","Isoleucyl-Threonine","Piperonyl sulfoxide","Fenpyroximate (Z,E)","PC(18:1(9Z)/18:1(9Z))","2-Acetoxy-3-geranylgeranyl-1,4-dihydroxybenzene","3-Hydroxybutyrylcarnitine (Car(4:0-O))","Bolegrevilol","LPC(17:0/0:0)","PG(18:3(6Z,9Z,12Z)/22:5(4Z,7Z,10Z,13Z,16Z))","Alvimopan","Phendimetrazine","Glycochenodeoxycholic acid","1-Palmitoylglycerol","N-Carbamoylputrescine","D-Urobilinogen","PC(16:0/14:1(9Z))","Oxybutynin","Pentylenetetrazole","Isonicotinic acid","LysoPE(0:0/18:0)","Spiroxamine","Montelukast","8,9-Dihydroxy-5Z,11Z,14Z-eicosatrienoic acid","N-(2,6-Dimethylphenyl)-1-piperazineacetamide","(E)-N-[2-(3-Indolyl)ethyl]-3-(4-hydroxy-3-methoxyphenyl)acrylamide","PS(22:4(7Z,10Z,13Z,16Z)/22:0)","4-Guanidinobutyric acid","1-Hexadecyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine","Adenosine 3'-monophosphate","Droperidol","MPL-dm","Cofaryloside","Esmolol","Blumenol_C_O-[apiosyl-(1->6)-glucoside]","2-Isopropyl-5-methylfuran","17,23-Epoxy-29-hydroxy-27-norlanost-8-ene-3,15,24-trione","Pentostatin","Bethanechol cation","PA(20:3(8Z,11Z,14Z)/15:0)","Propylpyrazoletriol","LysoPC(20:4(5Z,8Z,11Z,14Z))","DG(22:2(13Z,16Z)/18:2(9Z,12Z)/0:0)","(4R)-4-((1R,3S,5S,7R,9S,10S,13R,14S,17R)-1,3,7-trihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoic acid","7-Formyldehydrothalicsimidine","L-Pyridosine","PC(20:0/24:0)","Avocadyne Acetate","Podophyllotoxin","Gabapentin","Celastrol","Nefiracetam (Translon)","alpha-Chaconine","Cohibin_D","Clomiphene","Prilocaine","9,12-Octadecadiynoic acid","(3beta,5alpha,9alpha,22E,24R)-5,9-Epidioxy-3-hydroxyergosta-7,22-dien-6-one","Linoleamide","Ile-Pro","H-Val-Tyr-OH","PC(36:5)","(3beta,5alpha,6alpha,7alpha,22E,24R)-5,6-Epoxyergosta-8,14,22-triene-3,7-diol","Serotonin","DG(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/15:0/0:0)","Duloxetine","Hydroxyprolyl-Proline","N1,N8-Diacetylspermidine","2-QUINOLINECARBOXYLIC ACID","Panaxatriol","Sorbitan_laurate","Pipericine","DG(20:5(5Z,8Z,11Z,14Z,17Z)/18:1(11Z)/0:0)","N-Acetylserotonin","butabarbital","Thujyl_19-trachylobanoate","Imidazol-1-yl-acetic acid","2-Phenylpropionaldehyde_dimethyl_acetal","Dimetridazole","Lewis_a_trisaccharide","Aminopicoline","Aciclovir","2-Furyl methyl ketone","DG(20:4(8Z,11Z,14Z,17Z)/24:1(15Z)/0:0)","Ginsenoside_Rh3","Butyl_(S)-3-hydroxybutyrate_[arabinosyl-(1->6)-glucoside]","4,4-Dimethyl-1-(4-(4-(pyrimidin-2-yl)piperazin-1-yl)butyl)piperidine-2,6-dione","(3beta,22R,23R,24S)-3,22,23-Trihydroxystigmastan-6-one","3,4-Dihydro-2,2,5,7,8-pentamethyl-2H-1-benzopyran-6-ol","PS(20:0/14:0)","N-(5-Methyl-3-oxohexyl)alanine","Betaxolol","alpha-Solanine","Bissulfine","LysoPC(0:0/18:0)","Moroxydine","LPE(18:1(9Z)/0:0)","beta-Guanidinopropionic acid","7-[(6-hydroxy-5,5,8a-trimethyl-2-methylidene-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl)methoxy]chromen-2-one","6,7-Dihydro-2-methyl-5H-cyclopenta[b]pyrazine","Imazamethabenz","Phe-Asn","Methocarbamol","Glycylalanylprolylmethionylphenylalanylvalinamide","14-hydroxy-3-((5-hydroxy-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-16-yl acetate","Nateglinide","Threoninyl-Proline","DIHYDROTANSHINONE I","Homocitrulline","Leucyl-Lysine","Heptadecanoyl_carnitine","Hematoporphyrin_I","Bis(2-ethylhexyl) phthalate","Gamma-Tocotrienol","Echothiophate","Capsi-amide","Chlorophacinone","1-Aminocyclopentanecarboxylic acid","4-aminobutyric acid betaine","Pyrimidinol","Promethazine","LPC(18:2/0:0)","Annosquamosin_B","Histidinol","9-Oxo-11-(3-pentyl-2-oxiranyl)-10E-undecenoic acid","PC(P-16:0/0:0)","Melibiose","Ala-Glu","Cadabicine","(3b,4b,11b,14b)-11-Ethoxy-3,4-epoxy-14-hydroxy-12-cyathen-15-al_14-xyloside","Betazole","Lysyl-Glycine","Pantethine","(R)-2-Hydroxysterculic_acid","Metharbital","Arsenobetaine","Myristoylcarnitine","Catechin_5,7,4'-trimethyl_ether","2-Aminopyridine","N-Acetylproline","Flibanserin","Maltotriose","Aconine","Fumonisin_B1","N5-acetyl-l-ornithine","1-[4-(2-Methoxyphenyl)-1-piperazinyl]-3-(1-naphthyloxy)-2-propanol","13-HOTE","(13R,14R)-7-Labdene-13,14,15-triol","Methyldopa","L-Valyl-L-phenylalanine","Nicotinamide","Metronidazole","Theophylline","beta-Citraurin_epoxide","PC(14:0/14:0)","Iressa","1,2-Dipalmitoleoyl-sn-glycero-3-phosphoethanolamine","3beta-3,24-Dihydroxy-9(11),12-oleanadien-30-oic_acid","1,2-Dilinoleoyl-sn-glycero-3-phosphoethanolamine","Octopamine, N-feruloyl-","alpha-TOCHOPHERYL ACETATE","Dorsomorphin","PC(34:3)","7-[2,6-dimethyl-8-(2-methylbutanoyloxy)-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid","Piperazine","Tridemorph","Picropodophyllotoxin","1-Myristoyl-sn-glycero-3-phosphocholine (LPC(14:0/0:0))","4',7-Di-O-methylcatechin","Phenothiazine","Nodakenitin","5-Amino-2-methoxyphenol","Rilmenidine","Setariol","Ganoderiol_I","S-Adenosylmethionine","N-[3-(2-oxopyrrolidin-1-yl)propyl]acetamide","Deoxyguanosine","Acetamide, 2-(4-benzoylphenoxy)-N-[1-(phenylmethyl)-4-piperidinyl]-","9-Methyl-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one","7,8-Dehydro-beta-micropteroxanthin","7,8-Dihydrovomifoliol_9-[apiosyl-(1->6)-glucoside]","Difenoxin","2-Pyridinemethanamine, N-[[4-(1,4,8,11-tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-","PE(18:2(9Z,12Z)/0:0)","PC(14:1(9Z)/14:1(9Z))","3-cis-Hydroxy-b,e-Caroten-3'-one","Xanthophyll","LPC(20:0)",".beta.-Hydroxyphenylalanine","Sph(t18:0)","Hericerin","5,9-Epidioxy-3-hydroxyergost-7-en-6-one","Cucurbitacin_B","Tramadol","Dihydroxyacidissiminol","2-(5-Methyl-2-furanyl)pyrrolidine","LPA(20:5)","Nicotinamide N-oxide","PC(14:0/16:0)","SIROLIMUS","DG(18:4(6Z,9Z,12Z,15Z)/24:1(15Z)/0:0)","AS_1-5","Erucamide","Canthaxanthin (Euglenanone)","Maltose","Reticulataxanthin","3-Methyl-3H-imidazo[4,5-f]quinoxalin-2-amine","Methoxetamine","1-Oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine","Miltefosine","Histamine","Betamethasone dipropionate","Trypanothione_disulfide","Resolvin_D1","5'-Deoxyadenosine","2-Piperidone","Erianin","Jervine","Oxycodone","Pterine","Normetanephrine","15-Ketoprostaglandin F2.alpha.","Prolyl-Threonine","3-(2-Aminoethylthio)-2-methylpropionic acid","Physangulide","PS(22:4(7Z,10Z,13Z,16Z)/20:0)","PE(16:0/18:3(6Z,9Z,12Z))","Proparacaine","Lucidone_A","1,3-Dimethyluracil","Pyrrolidine","calcitetrol","Lucidone_B","Pubesenolide","LPA(0:0/18:0)","L-?Leucyl-?L-?alanine","Oxononenoylcarnitine (Car(9:2-O))","Terfenadine","Acebutolol","4-Hydroxy-16,18-tritriacontanedione","Synephrine","Anisomycin","(2E,4E)-2,4-Octadien-1-ol","Isatin","tomatidine","17.beta.-Nandrolone decanoate","Phe-Ala","Boviquinone_4","Methylthiomethyl_hexanoate"],"additional_accession":[]},"is_claimable":false,"name":"Terminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota","description":"Background: Aconitum kusnezoffii Reichb. is a commonly used medicinal herb in Mongolian medicine, a branch of traditional Chinese medicine. Processing A. kusnezoffii with a decoction of Terminalia chebula Retz. represents a characteristic and widely applied detoxification method in Mongolian medicine practice. Previous studies have demonstrated that this processing technique can reduce the toxicity of A. kusnezoffii. However, the underlying mechanisms remain insufficiently understood.
Methods: Normal Sprague-Dawley (SD) rats were randomly divided into the control (Ctrl), raw A. kusnezoffii (SCW), and Terminalia chebula decoction-processed A. kusnezoffii (HZZCW) groups. The metabolic profiles of rat feces and intestinal microbiota were detected. Hematoxylin and eosin (H&E) staining and enzyme-linked immunosorbent assay (ELISA) were performed to verify the toxicity of A. kusnezoffii. Western blotting (WB) and RT-qPCR were used to assess the expression of the PI3K/AKT signaling pathway. In addition, a collagen-induced arthritis (CIA) model was established to evaluate the anti-inflammatory effect.
Results: Metabolomic analysis revealed that processing A. kusnezoffii with Terminalia chebula decoction significantly altered metabolic pathways, particularly those related to ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism. The results of 16S rDNA sequencing showed that the processed product markedly changed the alpha-diversity of the gut microbiota and reduced the relative abundance of Xanthomonas and Corynebacterium. Pharmacodynamic evaluation indicated that HZZCW reduced the toxicity of raw A. kusnezoffii by regulating the levels of malondialdehyde (MDA), lactate dehydrogenase (LDH), and superoxide dismutase (SOD), while activating the PI3K/AKT pathway to maintain its therapeutic efficacy. Furthermore, the CIA model confirmed its significant anti-inflammatory activity.
Conclusion: Terminalia chebula decoction-processed A. kusnezoffii may exert its pharmacodynamic effects by modulating ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism, regulating intestinal microbiota, and activating the PI3K/AKT signaling pathway.
","dates":{"publication":"2026-06-27","submission":"2026-06-27"},"accession":"MTBLS14867","cross_references":{"HMDB":["HMDB0011500","HMDB0010407","HMDB0000163","HMDB0008097","HMDB0000062","HMDB0001522","HMDB0028686","HMDB0000064","HMDB0029010","HMDB0240650","HMDB0002064","HMDB0000301","HMDB0000875","HMDB0029068","HMDB0004826","HMDB0060665","HMDB0010382","HMDB0029130","HMDB0254473","HMDB0247297","HMDB0001262","HMDB0000235","HMDB0003459","HMDB0061715","HMDB0031641","HMDB0000030","HMDB0000043","HMDB0247960","HMDB0000244","HMDB0011506","HMDB0028988","HMDB0010390","HMDB0029133","HMDB0245980","HMDB0030386","HMDB0003701","HMDB0004620","HMDB0000870","HMDB0242109","HMDB0000898","HMDB0000699","HMDB0002284","HMDB0004159","HMDB0004827","HMDB0000097","HMDB0029139","HMDB0029140","HMDB0000865","HMDB0029134","HMDB0008038","HMDB0014341","HMDB0000252","HMDB0001406","HMDB0000045","HMDB0029065","HMDB0000259","HMDB0010386","HMDB0006750","HMDB0004095","HMDB0000897","HMDB0000050","HMDB0001983","HMDB0001173","HMDB0256387","HMDB0012252","HMDB0001185","HMDB0000902","HMDB0028702","HMDB0003464","HMDB0030352","HMDB0012136","HMDB0243890","HMDB0012108","HMDB0013337","HMDB0007866","HMDB0000562","HMDB0000269","HMDB0061384","HMDB0302434","HMDB0011749","HMDB0062769","HMDB0029217","HMDB0004437","HMDB0030396","HMDB0033971","HMDB0010379","HMDB0000303","HMDB0002894","HMDB0032538","HMDB0000501","HMDB0005066","HMDB0013222","HMDB0013751","HMDB0000167","HMDB0002141","HMDB0000306","HMDB0249045","HMDB0001238","HMDB0061933","HMDB0000101","HMDB0000177","HMDB0007869","HMDB0003282","HMDB0007970","HMDB0007982","HMDB0246162","HMDB0000854","HMDB0000206","HMDB0028922","HMDB0008031","HMDB0094708","HMDB0244507","HMDB0029645","HMDB0001538","HMDB0008034","HMDB0000593","HMDB0000048","HMDB0029120","HMDB0013302","HMDB0004610","HMDB0062656","HMDB0001539","HMDB0003431","HMDB0094696","HMDB0000921","HMDB0001325","HMDB0041947","HMDB0000819","HMDB0061712","HMDB0002144","HMDB0247667","HMDB0002730","HMDB0036584","HMDB0013643","HMDB0240288","HMDB0014634","HMDB0000222","HMDB0000900","HMDB0014642","HMDB0001861","HMDB0254682","HMDB0006050","HMDB0010384","HMDB0011600","HMDB0246517","HMDB0000937","HMDB0003540","HMDB0001565","HMDB0000085","HMDB0014935","HMDB0035248","HMDB0035289","HMDB0031849","HMDB0008821","HMDB0249821","HMDB0000917","HMDB0255618","HMDB0006023","HMDB0004948","HMDB0004326","HMDB0014535","HMDB0010697","HMDB0000716","HMDB0002719","HMDB0000054","HMDB0245222","HMDB0029736","HMDB0003581","HMDB0034885","HMDB0256585","HMDB0242684","HMDB0002825","HMDB0001860","HMDB0001889","HMDB0004339","HMDB0004950","HMDB0006695","HMDB0014925","HMDB0001859","HMDB0004400","HMDB0000881","HMDB0000079","HMDB0247518","HMDB0014417","HMDB0013127","HMDB0000992","HMDB0251409","HMDB0004949","HMDB0000216","HMDB0034252","HMDB0000679","HMDB0001893","HMDB0002392","HMDB0245129","HMDB0031464","HMDB0060362","HMDB0244322","HMDB0002095","HMDB0258715","HMDB0015020","HMDB0009093","HMDB0249363","HMDB0011730","HMDB0028844","HMDB0301778","HMDB0060525","HMDB0014564","HMDB0033249","HMDB0254597","HMDB0030701","HMDB0252427","HMDB0245307","HMDB0248283","HMDB0252014","HMDB0034530","HMDB0256829","HMDB0038244","HMDB0015055","HMDB0254835","HMDB0031858","HMDB0030525","HMDB0015519","HMDB0014869","HMDB0015202","HMDB0011152","HMDB0247377","HMDB0038300","HMDB0011149","HMDB0039528","HMDB0039588","HMDB0000842","HMDB0015490","HMDB0007973","HMDB0015038","HMDB0041959","HMDB0258215","HMDB0008727","HMDB0031710","HMDB0008308","HMDB0008100","HMDB0257360","HMDB0039148","HMDB0254733","HMDB0251597","HMDB0014333","HMDB0037381","HMDB0002169","HMDB0015240","HMDB0000802","HMDB0008923","HMDB0008002","HMDB0002759","HMDB0034702","HMDB0041583","HMDB0253709","HMDB0041806","HMDB0060374","HMDB0250755","HMDB0007890","HMDB0007881","HMDB0249406","HMDB0253141","HMDB0250490","HMDB0061007","HMDB0014597","HMDB0255737","HMDB0246202","HMDB0002099","HMDB0040044","HMDB0039986","HMDB0240552","HMDB0040015","HMDB0254454","HMDB0030372","HMDB0040749","HMDB0036394","HMDB0245964","HMDB0246913","HMDB0041519","HMDB0035735","HMDB0041451","HMDB0002201","HMDB0011754","HMDB0015114","HMDB0060655","HMDB0002227","HMDB0031770","HMDB0014339","HMDB0245733","HMDB0014888","HMDB0243530","HMDB0062225","HMDB0036604","HMDB0029168","HMDB0256533","HMDB0254828","HMDB0251622","HMDB0038288","HMDB0341277","HMDB0033206","HMDB0011174","HMDB0256296","HMDB0003012","HMDB0037148","HMDB0013336","HMDB0250642","HMDB0243942","HMDB0254922","HMDB0004063","HMDB0041802","HMDB0245482","HMDB0002249","HMDB0032116","HMDB0041500","HMDB0341362","HMDB0035113","HMDB0029747","HMDB0036695","HMDB0037382","HMDB0002226","HMDB0035766","HMDB0032468","HMDB0040656","HMDB0249183","HMDB0034198","HMDB0251509","HMDB0001161","HMDB0032565","HMDB0034301","HMDB0246412","HMDB0041253","HMDB0041940","HMDB0035196","HMDB0038249","HMDB0031810","HMDB0005015","HMDB0240291","HMDB0014619","HMDB0251590","HMDB0004992","HMDB0245026","HMDB0015095","HMDB0254275","HMDB0254889","HMDB0248436","HMDB0249630","HMDB0033910","HMDB0257232","HMDB00618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