MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14867/m_MTBLS14867_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14867/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14867/a_MTBLS14867_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14867/s_MTBLS14867.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14867<p>Metabolite feature identification and qualitative matching were performed using an in-house R package. Metabolite annotation and identification were achieved by comparison against the BiotreeDB database (V3.0).</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p>Chromatographic separation was performed using a Waters ACQUITY UPLC BEH Amide column (2.1 mm × 50 mm, 1.7 μm). Mobile phase A consisted of 25 mmol/L ammonium acetate and 25 mmol/L ammonia in ultrapure water, and mobile phase B was acetonitrile. Gradient elution was performed as follows: 0–0.25 min, 95% B; 0.25–3.5 min, 95%–65% B; 3.5–4.0 min, 65%–40% B; 4.0–4.5 min, 40% B; 4.5–4.55 min, 40%–95% B; and 4.55–6.0 min, 95% B. The column temperature was maintained at 25°C, the autosampler temperature was set at 4°C, the flow rate was 0.5 mL/min, and the injection volume was 2 μL.</p>Terminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota.Peking UniversityDuo Keaifecesmass spectrometry assay<p>A 25 mg sample was weighed under low-temperature conditions and transferred into an EP tube. Two homogenization beads were added, followed by 500 μL of extraction solvent consisting of methanol, acetonitrile, and water at a ratio of 2:2:1 (v/v/v). The extraction solvent contained isotope-labeled internal standards. The mixture was vortexed for 30 s and then homogenized using a tissue homogenizer at 35 Hz for 4 min. Subsequently, the sample was subjected to ultrasonic extraction in an ice-water bath for 5 min. This procedure was repeated three times. The sample was then incubated at -40°C for 1 h, followed by centrifugation at 12,000 rpm at 4°C for 15 min, corresponding to a centrifugal force of 13,800 × g with a rotor radius of 8.6 cm. The supernatant was collected and transferred into an injection vial for instrumental analysis.</p>Rattus norvegicushttps://www.ebi.ac.uk/metabolights/MTBLS14867Song Lin Song. Inner Mongolia Medical University. songlinwps@163.com.Sarina SA. Inner Mongolia Medical University. 1969135715@qq.com.<p>Raw metabolomics data were converted and preprocessed using a standard metabolomics data-processing workflow. First, outlier filtering was performed based on relative standard deviation/coefficient of variation (RSD/CV) to remove noise. Second, missing-value filtering was conducted, and only peak-area data with no more than 50% missing values within a single group and no more than 50% missing values overall were retained. Third, missing values were imputed using one-half of the minimum value. Fourth, data normalization and standardization were performed based on internal standards (IS). After conversion and preprocessing, 33,012 features were retained from the original 46,630 peaks for subsequent analysis.</p>Groupkeaiduoduo998@126.com<p>Fresh fecal samples were collected from experimental Sprague-Dawley (SD) rats on November 10, 2024. The samples were immediately collected under aseptic conditions, with visible impurities removed, and rapidly aliquoted into sterile cryopreservation tubes. After collection, the samples were immediately stored in a -80°C ultra-low-temperature freezer for long-term preservation until subsequent metabolomic analysis.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsA. kusnezoffiiuntargeted analysisThermo Scientific Orbitrap Exploris 120Rattus norvegicusTerminalia chebula decoction processing; metabolomicsfecesintestinal floraPI3K/Aktpharmacodynamic effectThermo Scientific Vanquish UHPLC SystemMetabolomicsA. kusnezoffiiuntargeted analysisThermo Scientific Orbitrap Exploris 120Rattus norvegicusTerminalia chebula decoction processing; metabolomicsfecesintestinal floraPI3K/Aktpharmacodynamic effect<p>Mass spectrometric analysis was performed using an Orbitrap Exploris 120 MS system equipped with an electrospray ionization (ESI) source. Data were acquired in both positive and negative ion modes. The sheath gas flow rate was set to 50 arb, and the auxiliary gas flow rate was set to 15 arb. The capillary temperature was maintained at 320°C. The MS resolution was 60,000, and the MS/MS resolution was 15,000.</p>7-hydroxy-2-methyl-3-phenyl-4H-chromen-4-one3-Hydroxy-b,e-caroten-3'-oneCaprolactamMalonyl-L-carnitineMangiferdesmethylursanone6alpha-methylprednisoloneMurrayamine_AN1,N10-Dicoumaroylspermidine4-CarboxyphenylglycinePC(18:0/15:0)EctoineVal-MetMethyl_6-O-galloyl-beta-D-glucopyranosideLysoPE(0:0/18:1(9Z))Norphenylephrine4-Methoxy-3-geranylgeranyl-1,2-dihydroxybenzene2-Keto-6-acetamidocaproateReserpineNormorphineCholest-4-en-3-one4-Hexylresorcinol1-Butanone, 1-(1,3-benzodioxol-5-yl)-2-(dimethylamino)-N-Methylcalystegine_C1Nipecotic acidCE(5:0)1-(1Z-Octadecenyl)-sn-glycero-3-phosphocholineGlutarylglycine4-Oxo-1,4-dihydro-3-quinolinecarboxylic acidbeta-Citraurol(4R)-4-((1R,3S,5S,7R,9S,10S,12S,13R,14S,17R)-1,3,7,12-tetrahydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoic acidN-EthylglycineHyocholic acidMeglumineD-Glucosaminic acidLysoPC(24:1(15Z))3-(Methylthio)-1-propeneDG(16:1(9Z)/15:0/0:0)PicolinamideN-Methylcalystegine_B2PE(20:3(8Z,11Z,14Z)/22:0)2-Hydroxy-3-(3,4-dihydroxyphenyl)propanamidePilocarpinePE(20:1(11Z)/14:1(9Z))Zanthobisquinolone3H-Pyrazol-3-one, 1,2-dihydro-2-[6-(4-morpholinyl)-4-pyrimidinyl]-4-(1H-1,2,3-triazol-1-yl)-Gamma-glutamyl-GlutamineBisphenol A diglycidyl etherLinalool_3,7-oxide_beta-primeverosideSecobarbitalPC(16:0/18:0)DG(18:2(9Z,12Z)/16:1(9Z)/0:0)Propionylpromazine (hydrochloride)AMP-DeoxynojirimycinDG(18:3(9Z,12Z,15Z)/15:0/0:0)PC(P-16:0/18:2(9Z,12Z))Ergostane-3,6-dione2-Hydroxy-6-tridecylbenzoic acidCurcumin_IIIThaumatin_b,_recombinantWithanolide(2beta,3alpha,9alpha,24R)-Ergosta-7,22-diene-2,3,9-triolLacCer(d18:1/16:0)N6-Methyladenine2-Propylthiazolo[4,5-c]quinolin-4-amineKainic acidPrednisolone_tebutateGln-ProHygric acidAuxin_aPhenylalanine betaineUrsocholic acid1-O-Hexadecyl-sn-glycero-3-phosphocholine (LPC(O-16:0/0:0))7-KetocholesterolXanthurenic acidPE(24:1(15Z)/P-16:0)SaquinavirBenzocaine1-(1Z-Octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamineBicifadine3-NitrophenylhydrazineMetalaxylSTIGMASTA-4,22-DIEN-3-ONEBrucinecyclopentolate25-Acetylvulgaroside1-Docosahexaenoyl-2-stearoyl-sn-glycero-3-phosphocholine5-MethoxytryptamineMupirocinProlylalanineHexadecadienoylcarnitine (Car(16:2))4beta-HydroxycholesterolMetoprolol acidGly-IleL-Glycine ether esterDesethyleneciprofloxacinMethylguanidinePE(22:4(7Z,10Z,13Z,16Z)/14:0)cis-7,10,13,16-Docosatetraenoic acidN-Oleoyltaurine3-MethyladenineMLS000859943-01!(8'R)-Neochrome4-Methyl-5-vinylthiazolePisumoside_B8-ShogaolLPE(P-16:0)(3beta,5alpha,6beta,22E,24R)-23-Methylergosta-7,22-diene-3,5,6-triolPromethazine sulfoxideN,N-Diethyl-2-aminoethanolIcaridin(3beta,17alpha,23S)-17,23-Epoxy-3,29-dihydroxy-27-norlanost-8-en-24-oneMasitinib (AB1010)Adenosine3,6,9,12-Tetraoxatetracosan-1-olL-tyrosine-methyl-esterOxyphenoniumPC(18:0/18:1(9Z))PA(20:4(5Z,8Z,11Z,14Z)/22:4(7Z,10Z,13Z,16Z))(3S,3'R,4xi)-beta,beta-Carotene-3,3',4-triolDesglucocorolosideHelinorbisabone5-MethylcytosinePC(15:0/P-18:0)SuillinFenpropathrin1h-indole-3-butanoic acidPhenforminDalfopristinNigellimine_N-oxide1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamineSphingosine(S)-(-)-Perillyl_alcohol3-Oxostearic acidMesaconineMETARAMINOLThreonine(2R)-3-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)propane-1,2-diyl ditetradecanoateLys-LeuDiferuloylputrescineN-Acetyl-arginineN-(3-PHENYLPROPIONYL)GLYCINEAlanyl-LeucineParacetamol (Drug)PhenylethylmalonamideLinoleoylcarnitine (Car(18:2))Heptaminol4-Amino-2-oxo-1,2-dihydropyrimidine-5-carboxylic acidCabergoline3-Aminohexanoic acid1,2-Dieicosenoyl-sn-glycero-3-phosphocholineNaphtho[2,3-b]furan-2(4H)-one, 4a,5,6,7,8,8a,9,9a-octahydro-3,8a-dimethyl-5-methylene-Thesinine_4'-O-glucosideAsperageninDulciol_AMG(22:5(4Z,7Z,10Z,13Z,16Z)/0:0/0:0)Phloionolic_acid2-O-Methylcytosine8,9-epoxy-3-isobutyryloxy-10-(2-methylbutanoyl)thymolMeperidine1-MethylnicotinamideEthyl 3-(N-butylacetamido)propionate(3beta,15alpha,22S,24E)-3,15,22-Trihydroxylanosta-7,9(11),24-trien-26-oic_acid1,25-Dihydroxyvitamin_D3-26,23-lactone2-(2-Furyl)piperidineOryzanol_APC(18:4(6Z,9Z,12Z,15Z)/14:0)1-(2-Thienyl)-1-heptanoneIsoxaben1-Aminocyclohexanecarboxylic_acidSyrosingopineN'-Nitrosonornicotine7-MethylguanineQuercetin_3-(2-caffeoylglucuronoside)GlycylserylprolylmethionylphenylalanylvalinamideDG(18:1(11Z)/20:3(5Z,8Z,11Z)/0:0)(3R,5S,7R,9S,10S,12S,13R,14S,17R)-17-((2R)-7-hydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triolQuinestrolMepirizole4-HydroxyvalsartanLysoPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z))Pipecolic acidpapaverineProlyl-Serine2-Amino-3-methoxybenzoic acidTestosterone_phenylpropionate1-MethylpiperazineLys-ProDG(20:5(5Z,8Z,11Z,14Z,17Z)/20:0/0:0)Ala-Pro2-EthylpyrazinePE(14:0/15:0)7H-Purine-7-acetamide, 1,2,3,6-tetrahydro-1,3-dimethyl-N-[4-(1-methylethyl)phenyl]-2,6-dioxo-CarbetapentaneMometasone_furoate2,4-Dimethoxyaniline3alpha-HydroxyoreadoneCarnitineProstaglandin_PGE2_1-glyceryl_esterPC(14:0/P-16:0)Anhydroecgonine methyl esterLPC(16:0)2-Amino-3,4,8-trimethyl-3H-imidazo[4,5-f]quinoxalineLenalidomide__(22E,24R)-Stigmasta-4,22-diene-3,6-dioneCilazaprilDiisooctyl phthalateAlanylalanine (Ala-Ala)AcetildenafilPC(20:4(8Z,11Z,14Z,17Z)/15:0)1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholineN6,N6,N6-TrimethyllysineMacrophorin_A2,4,6-Trimethylpyridine8-DesoxygartaninGramineStercobilinglycyl-prolineTrifluoroperazineTOBRAMYCIN3-Methylhistamineschisandrin BPC(18:1(9Z)/P-18:0)2-Propene-1-thiolapo-[3-methylcrotonoyl-CoA:carbon-dioxide_ligase_(ADP-forming)]1-MethylhistamineValenciachromeCar(18:1)AdluponePE(16:0/15:0)Androsterone_sulfateCamellenodiolTrimethobenzamideDG(22:2(13Z,16Z)/20:3(5Z,8Z,11Z)/0:0)(25R)-3alpha,7alpha,12alpha,24S-tetrahydroxy-5beta-cholestan-26-oic_acidalpha-AmyroneSph(d18:0)Trigonelline(.+/-.)-Dropropizine3-Hydroxy-carbofuran(3beta,5alpha,6beta,9alpha,22E,24R)-23-Methylergosta-7,22-diene-3,5,6,9-tetrolursolic acidPentoxifyllineCyclohexaneacetic_acidIsoleucylvalinePonatinib_(AP24534)(E)-Avenanthramide_D6-methyltetrahydropterinN-nornuciferineDG(24:0/18:4(6Z,9Z,12Z,15Z)/0:0)Fenfluramine2,5-DimethylpyrazineTangeraxanthinVal-IleHordatine_A4-(Dimethylamino)pyridine(S)-SpinacineMomorcharaside_BCAPSAICINm-HydroxybenzoylecgonineCYCLOPENTANONEMofebutazone3-Methyl-.gamma.-butyrolactone3-HydroxylidocaineProlylhydroxyprolinePlantaricin_BNButalbitalGitogeninXanthoxylinMyosmine2'-Deoxyinosine-5'-triphosphate trisodium saltTryptamine2-hydroxyphytanic_acidHexobarbitoneTemsirolimus2-Methylpropanamine7-ethenyl-1,4a,7-trimethyl-3,4,6,8,8a,9,10,10a-octahydro-2H-phenanthrene-1-carboxylic acidPromazineCreatineMinoxidil(4OH,8Z,t18:1)_sphingosine3-hydroxy-sebacic_acidNAE(18:2)Palmitoleylcarnitine (Car(16:1))2,3,4,5-Tetrahydro-6-(5-methyl-2-furanyl)pyridineSchleicherastatin_3OctinoxateDihydrorhodamine 1233-SuccinoylpyridineTolazoline (hydrochloride)Bisoprolol7,8-DihydroxyflavoneMaltoxazineN2-(1-Oxo-4-phenylbutyl)-L-glutamine2-HydroxyenterodiolBenzoylaconine2-Imino-1-imidazolidineacetic acidCarvedilolN,N-Dimethylarginine (ADMA)Cyclo(glycylprolyl)PehanormArachidyl_carnitinePE(P-36:4)Rubraflavone_CDesmosterolPermetin_AVal-ValLPC(18:0/0:0)N-Acetyl-D-glucosaminyldiphosphodolicholEthephonRhizonin_ACeritinibPiperanineHarmaline2,5-Dimethyl-3-(1-propenyl)pyrazineSpirotaccageninCDP-DG(18:0/22:3(10Z,13Z,16Z))Falimint1-Hydroxy-3,6,7-trimethoxy-2,8-diprenylxanthone5-[[17-(5,6-dihydroxy-6-methylheptan-2-yl)-3,12-dihydroxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-2-yl]oxy]-3-hydroxy-3-methyl-5-oxopentanoic acidAmobarbital15-formyl-16-hydroxy-1,2,14,17,17-pentamethyl-8-(prop-1-en-2-yl)pentacyclo[11.7.0.0,.0,.0,]icosane-5-carboxylic acid1-Deoxy-D-glucitolNorepinephrineDHA ethyl esterQuassimarin1-O-trans-cinnamoyl-beta-D-glucopyranoseGarcimangosone_APE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)Dimethylethanolamine1-Hexadecyl lysophosphatidic acid12,13-Epoxy-9,15-octadecadienoic_acidPiperazine, 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)-1,3-Bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]ureaHistidineTetranorprostaglandin J2CamelledionolAscaridolePE(22:2(13Z,16Z)/0:0)TyraminePC(14:0/15:0)QuinaprilatPalmitoylcarnitine (Car(16:0))Arg-Pro-Pro9E,11E-Octadecadienoic acid2-Hexenoylcholine1-alpha-AcevaltrateDeoxyadenosineMelezitosePE(22:1(13Z)/P-16:0)2-Fluoro-7H-purin-6-amineAmikacinAla-SerPulcherosineLysoPE(0:0/22:0)Cholesta-4,6-dien-3-oneTrilostanePhysalin_LSulpirideAnnocherin_AThr-LeuCyanidin-3-O-sambubioside-5-O-glucosideHydrocortamate1,9-NonanedithiolGanodosteroneMLS001304073-01!Prednisone53-03-23-Pyridylacetic acid2-DeoxysepiapterinFrangulanineGlaucarubinButyl_oleate_sulfateDodecanoic acid, 12-[[(tricyclo[3.3.1.13,7]dec-1-ylamino)carbonyl]amino]-1-Isothiocyanato-4-phenylbutaneTyrosyl-TyrosineN-AcetylputrescineTryptophanFluvoxamine_acidDG(20:3(5Z,8Z,11Z)/20:4(8Z,11Z,14Z,17Z)/0:0)GlycyrrhetinatePC(16:0/20:4)Choline1,2-Dioleoyl-rac-glycerol(3b,6b,8a,12a)-8,12-Epoxy-7(11)-eremophilene-6,8,12-trimethoxy-3-olLysoPC(14:1(9Z))Tanshinone IiaTrandolaprilat(8R,8'R)-Secoisolariciresinol_9-glucosidePE(20:4(5Z,8Z,11Z,14Z)/0:0)3-[Hexadecyl(dimethyl)ammonio]-1-propanesulfonate2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphoserine1-isothiocyanato-8-methanesulfinyloctane1-Aminopropan-2-olIlicifolinoside_ASoyasaponin_IIIAniline(9S,10S)-9,10-dihydroxyoctadecanoic_acidAdenosine monophosphate (AMP)12'-ApozeaxanthinCer(d18:1/18:1(11Z))Dulcino-TyrosineHydrocodone1,2-Di(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamineNeryl_glucosidePhosphorylcholineIdoxanthinCepagenin(-)-trans-4-[4-(4'-Fluorophenyl)-3-piperidinylmethoxy]-2-methoxyphenolHMMNIMildronateAcetamide, 2-[4-[(5,6-diphenyl-2-pyrazinyl)(1-methylethyl)amino]butoxy]-N-(methylsulfonyl)-FenamiphosLysoPC(22:1(13Z))DihydrothymineMyricolalPiperidinePanaxynolPro-ProArg-Ala9S,11R-Dihydroxy-15-oxo-13E-prostenoic acidOlomoucine(3beta,22E,24R)-Ergosta-4,6,8(14),22-tetraen-3-olDethiobiotinArecaidinePG(16:1(9Z)/18:3(9Z,12Z,15Z))Tetramethylquercetin_3-rutinosideNorbuprenorphineCer(d18:1/18:1(9Z))trans-S-(1-Propenyl)-L-cysteine1,7-DimethylxanthineMethohexitalThr-PheDoxazosin1-O-Octadecyl-sn-glyceryl-3-phosphorylcholineN-JasmonoyltyrosineIndicanRiboflavinMeAIBclobenzorexErgocalciferol (Vitamin D2)Anhydroamarouciaxanthin_BUrolithin BIsoleucylleucineN-DesmethyltramadolLeucine methyl ester(9R,10S,12Z)-9,10-Dihydroxy-8-oxo-12-octadecenoic_acidp-AcetaminobenzaldehydePA(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))(6beta,24R)-6-Hydroxystigmast-4-en-3-oneCamelininPholcodineLys-ValDG(22:5(4Z,7Z,10Z,13Z,16Z)/20:3(5Z,8Z,11Z)/0:0)DG(22:4(7Z,10Z,13Z,16Z)/22:2(13Z,16Z)/0:0)Acetic acid, 2-[4-[(5,6-diphenyl-2-pyrazinyl)(1-methylethyl)amino]butoxy]-(14alpha,17beta,20S,22R)-14,20-Epoxy-17-hydroxy-1-oxowitha-3,5,24-trienolideHarman1-Isothiocyanato-7-(methylthio)heptane5-O-MethylembelinDG(18:4(6Z,9Z,12Z,15Z)/15:0/0:0)2-Methyl-4'-(methylthio)-2-morpholinopropiophenoneBiotinKukoamine BBuflomedilLigustrazinePhysapubescinAtrazine desethylArmillane(2E,4E,12Z)-N-(2-methylpropyl)octadeca-2,4,12-trienamideDHAP(18:0e)alpha-Tocopherol (Vitamin E)Cholestan-3-OnePC(16:1(9Z)/0:0)[U]Homoveratrylamine4-HydroxynornantenineDinotefuranLiensinineetheno-dADicyclomine2'-O-Methyladenosine2,6-DimethylpyrazineAnastrozole(3beta,23E)-3-Hydroxy-27-norcycloart-23-en-25-oneMiglitolDMPON-AcetylvanilalanineMLS001077323-01!51333-22-32,3-Butanediol_apiosylglucoside2-Dimethylamino-6-hydroxypurineLuminespib1-O-Hexadecyl-2-O-(5Z,8Z,11Z,14Z,17Z-eicosapentaenoyl)-sn-glyceryl-3-phosphorylcholineN-AcetylcadaverineIfenprodilPC(18:0/14:0)DG(22:5(7Z,10Z,13Z,16Z,19Z)/22:2(13Z,16Z)/0:0)LysoPE(16:1(9Z)/0:0)Isopentenyl-AdenineCer(d18:0/16:0)Butorphanol3-Hydroxyhexadecanoylcarnitine5-Acetylamino-6-amino-3-methyluracil (AAMU)N-[(4E,8E)-1,3-dihydroxyoctadeca-4,8-dien-2-yl]hexadecanamidePC(15:0/20:1(11Z))Bullatine BLysoPC(18:1(11Z))2-Methyl-5-HTN2-(3-Carboxy-2-hydroxy-1-oxopropyl)argininePiracetamDi-4-coumaroylputrescine1,2-Dipalmitoleoyl-sn-glycero-3-phosphocholineisepamicin4,8_dimethylnonanoyl_carnitineEnterocin_900trans-ZeatinPC(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:5(4Z,7Z,10Z,13Z,16Z))PC(P-32:0)PC(15:0/18:3(9Z,12Z,15Z))14-(hydroxymethyl)-5,9-dimethyltetracyclo[11.2.1.0,.0,]hexadecane-5-carbaldehydeHecogeninSpiperonePalmitoyl sphingomyelinPC(18:0/16:0)L-Thyronine1-MethylguanineCer(d18:0/14:0)PindololDG(22:2(13Z,16Z)/20:4(5Z,8Z,11Z,14Z)/0:0)(3R,7R,9S,10S,12S,13R,14S,17R)-17-((2R)-6,7-dihydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,7,12-triol1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine12-Ketoporrigenin5',5''',8,8''-Tetrahydroxy-3',3''',4',4''',7',7''-hexamethoxy-5,5''-biflavanHeneicosanoic acid (21:0)3alpha,4,7,7alpha-Tetrahydro-4-hydroxy-1H-isoindole-1,3(2H)-dionePhenylalanyl-Glycine2,4-Diamino-6-nitrotolueneGalunisertibNotoginsenoside_JStigmasterolMethiocholic acid putativePesticide3_Carbofuran_C12H15NO3_FuradanDiethanolamineCeruleninThiamineSoyasapogenol B base + O-HexA-Hex-Pen, O-C6H7O3(DDMP)PC(20:2(11Z,14Z)/15:0)Chlordiazepoxide5-(12-Heneicosenyl)-1,3-benzenediolleonurine3,8-dihydroxy-3,8-dimethyl-5-propan-2-ylidene-1,2,3a,4,7,8a-hexahydroazulen-6-one3,6-Ditigloyloxytropan-7-olMethdilazineOlaquindoxPhenylalanylphenylalanineDaltogenN6-AcetyllysineN-[2-(4-Prenyloxyphenyl)ethyl]tiglamide1-[1,4-Dihydro-4-nonyl-5-(1-oxodecyl)-3-pyridinyl]-1-dodecanoneNorverapamilbetulinLyciumoside_I2-Aminonaphthalene3'-O-MethyladenosineN-Oleoyl-D-erythro-sphinganineDovitinibElastinSpectinomycinSterebin_CSterebin_APE(P-16:0/22:0)Urocanic acid1-Hydroxy-3-methyl-9H-carbazoleCer(d18:0/18:0)Pirenperone5-Norbornene-2,3-dicarboximide, N-(2-ethylhexyl)-DG(20:4(5Z,8Z,11Z,14Z)/18:1(11Z)/0:0)Piperolactam_DNeferineMetanephrine2,3-Diethylpyrazine2-AminopimelateNicotinamide adenine dinucleotide (NAD)Rotundine2-Amino-5-phenylpyridineTetraethylene glycol3-Pyridinemethanol5'-MethylthioadenosinePC(24:1(15Z)/22:2(13Z,16Z))Cer(d18:1/16:0)EremanthinAcetophenone1,2-Diarachidonoyl-sn-glycero-3-phosphocholinePriverogenin_A2-HydroxypyridineButenafineCreatinineAntibiotic_X_14889C4-ButyloxazoleQuinoline, 4-[6-[4-(1-piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]-PC(15:0/22:2(13Z,16Z))Madlongiside_DCyproheptadineFormiminoglutamic acid7alpha,12alpha,26-trihydroxycholest-4-en-3-oneStachydrine7',8'-Dihydro-8'-hydroxycitraniaxanthinbeta-TocotrienolCarisoprodolPG(20:4(5Z,8Z,11Z,14Z)/20:2(11Z,14Z))Paromomycin8R-HpODECoenzyme_Q4TheobromineBilirubin[8]-ParadolCrispolide2-(10-Heptadecenyl)-6-hydroxybenzoic_acidArgininic_acidBetaineLuminolN,N-DiethylcathinoneFormylfusarochromanoneGinsenoyne_A_linoleatePhenacetinCer(d18:1/18:0)Sarcodon_scabrosus_DepsipeptideN-Carboxyethyl-r-aminobutyric_acidManglupenoneMesaconitine1-(1Z-Octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine5,8-Epoxy-5,8-dihydro-10'-apo-b,y-carotene-3,10'-diol1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N,N-dimethylABOADOCOSANOLEthyl 3-aminobenzoatehexadecanedioic_acid_mono-L-carnitine_ester1-Stearoyl-2-docosahexaenoyl-sn-glycerol4-Megastigmene-6a,9R-diol_9-[apiosyl-(1->6)-glucoside]CandoxatrilSuccinamideMesobilirubinogenGoyaglycoside_gLPE(14:0)5-Methyl DL-glutamatePhytoCer(18:0/16:0)(2alpha,3beta)-2,3-dihydroxyolean-12-en-28-oic acidSuxibuzoneH-Val-Ala-OHAliskirenMusanolone_FDimethylbenzimidazoleIsoleucyl-ThreoninePiperonyl sulfoxideFenpyroximate (Z,E)PC(18:1(9Z)/18:1(9Z))2-Acetoxy-3-geranylgeranyl-1,4-dihydroxybenzene3-Hydroxybutyrylcarnitine (Car(4:0-O))BolegrevilolLPC(17:0/0:0)PG(18:3(6Z,9Z,12Z)/22:5(4Z,7Z,10Z,13Z,16Z))AlvimopanPhendimetrazineGlycochenodeoxycholic acid1-PalmitoylglycerolN-CarbamoylputrescineD-UrobilinogenPC(16:0/14:1(9Z))OxybutyninPentylenetetrazoleIsonicotinic acidLysoPE(0:0/18:0)SpiroxamineMontelukast8,9-Dihydroxy-5Z,11Z,14Z-eicosatrienoic acidN-(2,6-Dimethylphenyl)-1-piperazineacetamide(E)-N-[2-(3-Indolyl)ethyl]-3-(4-hydroxy-3-methoxyphenyl)acrylamidePS(22:4(7Z,10Z,13Z,16Z)/22:0)4-Guanidinobutyric acid1-Hexadecyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamineAdenosine 3'-monophosphateDroperidolMPL-dmCofarylosideEsmololBlumenol_C_O-[apiosyl-(1->6)-glucoside]2-Isopropyl-5-methylfuran17,23-Epoxy-29-hydroxy-27-norlanost-8-ene-3,15,24-trionePentostatinBethanechol cationPA(20:3(8Z,11Z,14Z)/15:0)PropylpyrazoletriolLysoPC(20:4(5Z,8Z,11Z,14Z))DG(22:2(13Z,16Z)/18:2(9Z,12Z)/0:0)(4R)-4-((1R,3S,5S,7R,9S,10S,13R,14S,17R)-1,3,7-trihydroxy-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoic acid7-FormyldehydrothalicsimidineL-PyridosinePC(20:0/24:0)Avocadyne AcetatePodophyllotoxinGabapentinCelastrolNefiracetam (Translon)alpha-ChaconineCohibin_DClomiphenePrilocaine9,12-Octadecadiynoic acid(3beta,5alpha,9alpha,22E,24R)-5,9-Epidioxy-3-hydroxyergosta-7,22-dien-6-oneLinoleamideIle-ProH-Val-Tyr-OHPC(36:5)(3beta,5alpha,6alpha,7alpha,22E,24R)-5,6-Epoxyergosta-8,14,22-triene-3,7-diolSerotoninDG(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/15:0/0:0)DuloxetineHydroxyprolyl-ProlineN1,N8-Diacetylspermidine2-QUINOLINECARBOXYLIC ACIDPanaxatriolSorbitan_lauratePipericineDG(20:5(5Z,8Z,11Z,14Z,17Z)/18:1(11Z)/0:0)N-AcetylserotoninbutabarbitalThujyl_19-trachylobanoateImidazol-1-yl-acetic acid2-Phenylpropionaldehyde_dimethyl_acetalDimetridazoleLewis_a_trisaccharideAminopicolineAciclovir2-Furyl methyl ketoneDG(20:4(8Z,11Z,14Z,17Z)/24:1(15Z)/0:0)Ginsenoside_Rh3Butyl_(S)-3-hydroxybutyrate_[arabinosyl-(1->6)-glucoside]4,4-Dimethyl-1-(4-(4-(pyrimidin-2-yl)piperazin-1-yl)butyl)piperidine-2,6-dione(3beta,22R,23R,24S)-3,22,23-Trihydroxystigmastan-6-one3,4-Dihydro-2,2,5,7,8-pentamethyl-2H-1-benzopyran-6-olPS(20:0/14:0)N-(5-Methyl-3-oxohexyl)alanineBetaxololalpha-SolanineBissulfineLysoPC(0:0/18:0)MoroxydineLPE(18:1(9Z)/0:0)beta-Guanidinopropionic acid7-[(6-hydroxy-5,5,8a-trimethyl-2-methylidene-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl)methoxy]chromen-2-one6,7-Dihydro-2-methyl-5H-cyclopenta[b]pyrazineImazamethabenzPhe-AsnMethocarbamolGlycylalanylprolylmethionylphenylalanylvalinamide14-hydroxy-3-((5-hydroxy-4-methoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-16-yl acetateNateglinideThreoninyl-ProlineDIHYDROTANSHINONE IHomocitrullineLeucyl-LysineHeptadecanoyl_carnitineHematoporphyrin_IBis(2-ethylhexyl) phthalateGamma-TocotrienolEchothiophateCapsi-amideChlorophacinone1-Aminocyclopentanecarboxylic acid4-aminobutyric acid betainePyrimidinolPromethazineLPC(18:2/0:0)Annosquamosin_BHistidinol9-Oxo-11-(3-pentyl-2-oxiranyl)-10E-undecenoic acidPC(P-16:0/0:0)MelibioseAla-GluCadabicine(3b,4b,11b,14b)-11-Ethoxy-3,4-epoxy-14-hydroxy-12-cyathen-15-al_14-xylosideBetazoleLysyl-GlycinePantethine(R)-2-Hydroxysterculic_acidMetharbitalArsenobetaineMyristoylcarnitineCatechin_5,7,4'-trimethyl_ether2-AminopyridineN-AcetylprolineFlibanserinMaltotrioseAconineFumonisin_B1N5-acetyl-l-ornithine1-[4-(2-Methoxyphenyl)-1-piperazinyl]-3-(1-naphthyloxy)-2-propanol13-HOTE(13R,14R)-7-Labdene-13,14,15-triolMethyldopaL-Valyl-L-phenylalanineNicotinamideMetronidazoleTheophyllinebeta-Citraurin_epoxidePC(14:0/14:0)Iressa1,2-Dipalmitoleoyl-sn-glycero-3-phosphoethanolamine3beta-3,24-Dihydroxy-9(11),12-oleanadien-30-oic_acid1,2-Dilinoleoyl-sn-glycero-3-phosphoethanolamineOctopamine, N-feruloyl-alpha-TOCHOPHERYL ACETATEDorsomorphinPC(34:3)7-[2,6-dimethyl-8-(2-methylbutanoyloxy)-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acidPiperazineTridemorphPicropodophyllotoxin1-Myristoyl-sn-glycero-3-phosphocholine (LPC(14:0/0:0))4',7-Di-O-methylcatechinPhenothiazineNodakenitin5-Amino-2-methoxyphenolRilmenidineSetariolGanoderiol_IS-AdenosylmethionineN-[3-(2-oxopyrrolidin-1-yl)propyl]acetamideDeoxyguanosineAcetamide, 2-(4-benzoylphenoxy)-N-[1-(phenylmethyl)-4-piperidinyl]-9-Methyl-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one7,8-Dehydro-beta-micropteroxanthin7,8-Dihydrovomifoliol_9-[apiosyl-(1->6)-glucoside]Difenoxin2-Pyridinemethanamine, N-[[4-(1,4,8,11-tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-PE(18:2(9Z,12Z)/0:0)PC(14:1(9Z)/14:1(9Z))3-cis-Hydroxy-b,e-Caroten-3'-oneXanthophyllLPC(20:0).beta.-HydroxyphenylalanineSph(t18:0)Hericerin5,9-Epidioxy-3-hydroxyergost-7-en-6-oneCucurbitacin_BTramadolDihydroxyacidissiminol2-(5-Methyl-2-furanyl)pyrrolidineLPA(20:5)Nicotinamide N-oxidePC(14:0/16:0)SIROLIMUSDG(18:4(6Z,9Z,12Z,15Z)/24:1(15Z)/0:0)AS_1-5ErucamideCanthaxanthin (Euglenanone)MaltoseReticulataxanthin3-Methyl-3H-imidazo[4,5-f]quinoxalin-2-amineMethoxetamine1-Oleoyl-2-palmitoyl-sn-glycero-3-phosphocholineMiltefosineHistamineBetamethasone dipropionateTrypanothione_disulfideResolvin_D15'-Deoxyadenosine2-PiperidoneErianinJervineOxycodonePterineNormetanephrine15-Ketoprostaglandin F2.alpha.Prolyl-Threonine3-(2-Aminoethylthio)-2-methylpropionic acidPhysangulidePS(22:4(7Z,10Z,13Z,16Z)/20:0)PE(16:0/18:3(6Z,9Z,12Z))ProparacaineLucidone_A1,3-DimethyluracilPyrrolidinecalcitetrolLucidone_BPubesenolideLPA(0:0/18:0)L-?Leucyl-?L-?alanineOxononenoylcarnitine (Car(9:2-O))TerfenadineAcebutolol4-Hydroxy-16,18-tritriacontanedioneSynephrineAnisomycin(2E,4E)-2,4-Octadien-1-olIsatintomatidine17.beta.-Nandrolone decanoatePhe-AlaBoviquinone_4Methylthiomethyl_hexanoatefalseTerminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota<p>Background: Aconitum kusnezoffii Reichb. is a commonly used medicinal herb in Mongolian medicine, a branch of traditional Chinese medicine. Processing A. kusnezoffii with a decoction of Terminalia chebula Retz. represents a characteristic and widely applied detoxification method in Mongolian medicine practice. Previous studies have demonstrated that this processing technique can reduce the toxicity of A. kusnezoffii. However, the underlying mechanisms remain insufficiently understood.</p><p>Methods: Normal Sprague-Dawley (SD) rats were randomly divided into the control (Ctrl), raw A. kusnezoffii (SCW), and Terminalia chebula decoction-processed A. kusnezoffii (HZZCW) groups. The metabolic profiles of rat feces and intestinal microbiota were detected. Hematoxylin and eosin (H&amp;E) staining and enzyme-linked immunosorbent assay (ELISA) were performed to verify the toxicity of A. kusnezoffii. Western blotting (WB) and RT-qPCR were used to assess the expression of the PI3K/AKT signaling pathway. In addition, a collagen-induced arthritis (CIA) model was established to evaluate the anti-inflammatory effect.</p><p>Results: Metabolomic analysis revealed that processing A. kusnezoffii with Terminalia chebula decoction significantly altered metabolic pathways, particularly those related to ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism. The results of 16S rDNA sequencing showed that the processed product markedly changed the alpha-diversity of the gut microbiota and reduced the relative abundance of Xanthomonas and Corynebacterium. Pharmacodynamic evaluation indicated that HZZCW reduced the toxicity of raw A. kusnezoffii by regulating the levels of malondialdehyde (MDA), lactate dehydrogenase (LDH), and superoxide dismutase (SOD), while activating the PI3K/AKT pathway to maintain its therapeutic efficacy. Furthermore, the CIA model confirmed its significant anti-inflammatory activity.</p><p>Conclusion: Terminalia chebula decoction-processed A. kusnezoffii may exert its pharmacodynamic effects by modulating ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism, regulating intestinal microbiota, and activating the PI3K/AKT signaling pathway.</p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