MetaboLights

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were identified by matching the accurate mass, retention time, and MS/MS fragmentation patterns against reference databases, including mzCloud, ChemSpider, and the Human Metabolome Database (HMDB). The mass tolerance for precursor ions was set to 5 ppm, and for fragment ions to 10 ppm. Metabolites with a confidence score above 80% were considered as valid identifications.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseChromatographic separation was performed on an Acclaim 120 C18 column (2.2 μm, 2.1 mm × 150 mm; Thermo Scientific) at 40°C. The mobile phase consisted of (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile. The gradient elution program was as follows: 0–1 min, 5% B; 1–10 min, 5–95% B; 10–12 min, 95% B; 12–12.1 min, 95–5% B; 12.1–14 min, 5% B. The flow rate was 0.3 mL/min, and the injection volume was 2 µL.Cross-genus quorum-sensing signal boosts Streptomyces activity in soil-borne disease suppression.qin xiaoSouth China Normal Universitycell supernatantmass spectrometry assayThe culture supernatant was collected by centrifugation at 10,000 g for 10 min at 4°C and filtered through a 0.22 μm aqueous membrane. The filtered supernatant was directly used as the sample for LC-MS analysis without further extraction. Quality control (QC) samples were prepared by pooling equal volumes of all individual samples.Streptomyces sp. 30177https://www.ebi.ac.uk/metabolights/MTBLS14907qin xiao. South China Normal University. 19854815383@163.com.qin xiao. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. Guangzhou, Guangdong Province, China. 19854815383@163.com.The raw mass spectrometry data were processed using Compound Discoverer software (version 3.3, Thermo Fisher Scientific) for peak detection, alignment, and filtering. Metabolite features were extracted based on accurate mass and retention time. Data were normalized by total ion current (TIC) before statistical analysis.Treatment19854815383@163.comSphingomonas sp. 06703 was activated on NA plates. A single colony was inoculated into NB medium and cultured at 30°C, 180 rpm until OD600 reached 1.0. Cells were collected by centrifugation at 5,000 g, washed twice with PBS buffer, and resuspended to OD600 = 1.0. The cell suspension was then transferred (1% inoculum) into M9 glucose medium and cultured at 30°C, 180 rpm. Glucose consumption was monitored daily using a glucose assay kit (glucose oxidase method). After complete glucose depletion on day 6, the culture was centrifuged at 10,000 g for 10 min, and the supernatant was filtered through a 0.22 μm aqueous membrane to obtain the primary metabolite. Spores of Streptomyces sp. 30177 were collected by washing with sterile water, and the mycelium was filtered through a 5 μm filter to obtain a spore suspension. The spores were inoculated (1% inoculum) into the primary metabolite of Sphingomonas sp. 06703 and cultured at 30°C, 180 rpm for 6 days. The control group was inoculated into M9 medium. After incubation, the growth of Streptomyces sp. 30177 was assessed by measuring wet cell weight. The culture was centrifuged at 10,000 g for 10 min at 4°C, and the supernatant was filtered through a 0.22 μm aqueous membrane to obtain the secondary metabolite. Primary and secondary metabolites from both strains were collected.Metabolomicscell supernatantMetabolomicsuntargeted analysisThermo Scientific Accela 1250 PumpStreptomycesSphingomonasThermo Electron PolarisQexperimental blankStreptomyces sp. 30177cell supernatantMetabolomicsuntargeted analysisThermo Scientific Accela 1250 PumpStreptomycesSphingomonasThermo Electron PolarisQexperimental blankStreptomyces sp. 30177Mass spectrometry was performed on a Q Exactive HF-X mass spectrometer equipped with a HESI-II probe. The mass spectrometer was operated in both positive and negative ion modes. The full scan range was 100-1500 m/z with a resolution of 120,000 at m/z 200. The spray voltage was 3.5 kV for positive mode and 3.0 kV for negative mode. The sheath gas flow rate was 40 arbitrary units, the auxiliary gas flow rate was 10 arbitrary units, and the capillary temperature was 320°C. The S-lens RF level was set to 50.falseMetabolomics analysis of Streptomyces sp. 30177 in response to Sphingomonas sp. 06703 supernatantThis study investigated the metabolomic response of Streptomyces sp. 30177 to cell-free supernatant of Sphingomonas sp. 06703. Untargeted LC-MS metabolomics was performed to identify differentially regulated metabolites and pathways involved in metabolic regulation and stress response.2026-07-232026-06-30MTBLS14907