MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/m_MTBLS14913_GC-MS_positive_capillary-column_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/s_MTBLS14913.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/a_MTBLS14913_GC-MS_positive_capillary-column.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/S_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/H_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/S_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/H_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/H_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913/FILES/RAW_FILES/S_3.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14913<p>Metabolite identification has not yet been performed. The raw data are provided as acquired.</p>MetaboLightsPublicGas Chromatography MS - positive - capillary-column<p>Chromatographic separation was performed using an Agilent 7890A GC System equipped with an Agilent 7693A autosampler. The analytes were separated on a HP-5ms GC column (30 m × 0.25 mm × 0.25 µm; Agilent Technologies). High-purity helium (99.999%) was used as the carrier gas at a constant flow rate of 1.0 mL/min. The GC oven temperature program was as follows: initial temperature 40°C held for 2 min, ramped to 280°C at a rate of 5°C/min, and held for 5 min. The injection port temperature was maintained at 250°C. Samples were injected in splitless mode with an injection volume of 1 µL (or SPME desorption mode as described in the Extraction protocol). The total run time was approximately 55 min.</p><p><br></p>Analysis of Volatile Organic Compounds (VOCs) in Streptomyces sp. 30177 treated with cell-free filtrate of Sphingomonas sp. 06703 using Headspace Solid-Phase Microextraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS).South China Normal Universityqin xiaoWhole cellmass spectrometry assay<p>The bacterial culture (50 µL) was spread on one side of PDA agar medium and incubated at 28°C for 5 days. For VOC analysis, an aliquot of the agar culture was transferred into a 20 mL headspace vial and sealed with a PTFE/silicone septum. The sample was equilibrated at 60°C for 10 min with agitation. A 65 µm PDMS/DVB SPME fiber (Supelco, Bellefonte, PA, USA) was then exposed to the headspace for 30 min at the same temperature. After extraction, the fiber was retracted and immediately inserted into the GC injection port for thermal desorption at 250°C for 5 min in splitless mode. Blank agar samples were analyzed between each batch to monitor background contamination.</p>Streptomyces sp. 30177https://www.ebi.ac.uk/metabolights/MTBLS14913Wang Dan. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. Guangzhou, Guangdong Province, China. wangdandelia@163.com.qin xiao. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences. Guangzhou, Guangdong Province, China. 19854815383@163.com.<p>Raw data files (.CDF) were exported from Agilent MassHunter software. The raw data were processed using Agilent MassHunter Qualitative Analysis for peak detection and integrated using the NIST mass spectral library for compound identification. Peak areas were normalized prior to statistical analysis.</p>Treatment19854815383@163.com<p>Please update this protocol descriptionSphingomonas sp. 06703 was activated on NA medium for 24 h, then transferred to MM medium and cultured for another 24 h. The culture was centrifuged at 12,000 × g for 10 min, and the supernatant was collected and filter-sterilized through a 0.22 µm aqueous membrane filter to obtain the cell-free fermentation broth of Sphingomonas sp. 06703. Spores of Streptomyces sp. 30177 were harvested by washing with sterile water, and the mycelium was filtered through a 5 µm filter to obtain a spore suspension. The spore suspension of Streptomyces sp. 30177 was mixed with the cell-free fermentation broth of Sphingomonas sp. 06703, and 50 µL of the mixture was spread on one side of PDA agar medium, except for the control plates (Streptomyces sp. 30177 only).</p>MetabolomicsMetabolomicsuntargeted metabolomicsGuangdong Academy of Agricultural Sciencesgas chromatography-mass spectrometryAgilent 7890A GC Systemuntargeted analysisStreptomycesSphingomonasAgilent 5977B Mass Selective DetectorMassHunterGC-MSheadspace solid-phase microextractionVolatile Organic Compoundsexperimental blankWhole cellStreptomyces sp. 30177MetabolomicsGuangdong Academy of Agricultural Sciencesuntargeted metabolomicsgas chromatography-mass spectrometryAgilent 7890A GC Systemuntargeted analysisStreptomycesSphingomonasAgilent 5977B Mass Selective DetectorMassHunterGC-MSheadspace solid-phase microextractionVolatile Organic Compoundsexperimental blankWhole cellStreptomyces sp. 30177<p>The GC system was coupled to an Agilent 5977B Mass Selective Detector (MSD). Electron ionization (EI) was used as the ion source with an ionization energy of 70 eV. The ion source temperature was maintained at 230°C, and the quadrupole temperature was set to 150°C. Data acquisition was performed in full-scan mode over the m/z range of 29–350 amu with a scan rate of 2.0 scans per second. The solvent delay was set to 2.37 minutes to protect the filament from solvent overload. The detector was operated in positive ion mode.</p>falseAnalysis of Volatile Organic Compounds (VOCs) in Streptomyces sp. 30177 treated with cell-free filtrate of Sphingomonas sp. 06703 using Headspace Solid-Phase Microextraction (HS-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS)This study aimed to analyze the volatile organic compounds (VOCs) produced by Streptomyces sp. 30177 in response to treatment with the cell-free filtrate of Sphingomonas sp. 06703. The cell-free filtrate was prepared by culturing Sphingomonas sp. 06703 in liquid medium, followed by centrifugation and filtration through a 0.22 µm membrane. The filtrate was then added to the culture of Streptomyces sp. 30177. After incubation, the headspace VOCs were extracted using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). The raw data were acquired and processed using Agilent MassHunter software. This study aims to reveal the metabolic interaction between these two bacterial species and provide insights into the chemical communication mechanisms in microbial communities.2026-07-232026-07-01MTBLS14913