MetaboLights

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The raw data acquired after mass spectrometric analysis were processed using MassHunter software for qualitative and quantitative analysis.MetaboLightsPublicGas Chromatography MS - alternating - low-polarityChromatographic separation was achieved on a DB-5MS capillary column (30 m x0.25 mm , 0.25 ?m). Helium served as the carrier gas and was maintained at a constant flow rate of 1.2 mL/min. The oven program started at 40?C with a 1-min hold, followed by heating to 100?C at 20?C/min and then to 300?C at 15?C/min. The final temperature was maintained at 300?C for 5 min.To investigate the effects of long-term continuous soil drought on the rhizosphere metabolic environment.Antwerp universityMiao Jiangsoilmass spectrometry assayFresh samples were weighed, immediately frozen in liquid nitrogen, and stored at -80?C. Before extraction, samples were freeze-dried and ground into fine powder. Each powdered sample (0.5 g) was extracted with 1 mL methanol/isopropanol/water (3:3:2, v/v/v), vortexed for 3 min, and sonicated for 20 min. After centrifugation at 12,000 rpm for 3 min at 4?C, the supernatant was collected, mixed with 20 ?L internal standard solution (10 ?g/mL), dried under nitrogen, and lyophilized. rhizospherehttps://www.ebi.ac.uk/metabolights/MTBLS14920Miao Jiang. Antwerp university. jiangmiao0203@163.com.Li Xiangnan. Chinese Academy of Sciences. lixiangnan@iga.ac.cn.Beemster Gerrit. Antwerp university.Mass spectral fingerprints of the samples were matched against reference spectra, and compounds were assigned based on matching scores. Retention index information was further used to refine metabolite annotation and reduce false-positive identification. Raw mass spectrometry data were processed with MassHunter quantitative analysis software. Selected quantifier ions were used for peak integration and correction to ensure reliable metabolite quantification.Treatmentjiangmiao0203@163.comFor rhizosphere soil collection, intact root systems were gently removed from the pots and shaken to eliminate loosely associated bulk soil. Six biological replicates were collected for each treatment. The soil firmly attached to the root surface, within an estimated 0-2mm distance, was defined as rhizosphere soil. This soil fraction was carefully dislodged with a sterile soft brush, placed in sterile tubes, and passed through a sterile 2-mm sieve after visible plant debris and stones had been removed.Metabolomicspooled quality control sampleMetabolomicsuntargeted metabolomicsuntargeted analysisrhizosphere metabolomicsAgilent 5977B MSDsoilsoil droughtrhizosphereAgilent 8890 GCexperimental samplepooled quality control sampleMetabolomicsuntargeted metabolomicsrhizosphere metabolomicsuntargeted analysisAgilent 5977B MSDsoilsoil droughtAgilent 8890 GCrhizosphereexperimental sampleMass spectrometric detection was performed using electron ionisation with an electron energy of 70 eV. The transfer line temperature was set to 280?C, the ion source temperature was set to 230?C, and the quadrupole temperature was set to 150?C. The carrier gas was helium.falseTo investigate the effects of long-term continuous soil drought on the rhizosphere metabolic environmentTo examine whether drought-conditioned microbiome restructuring coincided with changes in rhizosphere metabolites, we profiled rhizosphere metabolomes across the three conditioning generations.2026-07-022026-07-02MTBLS14920