Lipid identification was achieved through
a spectral match using LipidBlast library, which was developed using R and based on
XCMS.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["For lipids, LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo
Fisher Scientific) with the Phenomenex Kinetex C18 (2.1 mm × 100 mm, 2.6 μm) coupled
to Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo). The mobile phase A was
H2O/ACN (6:4, v/v) with 10 mM HCOONH4, and mobile phase B was IPA/ACN (9:1, v/v) with 10
mM HCOONH4. The injection volume was 2 μL.
"],"publication":["ER fusogens maintain membrane reservoir to ensure brain function."],"submitter_affiliation":["Institute of biophysics, Chinese academy of sciences"],"submitter_name":["hanchi miao"],"organism_part":["kidney"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The cell pellets were mixed with beads and 200 μl H2O and underwent three freeze–thaw cycles. The samples were mixed with 480 μl of extraction solution [MTBE:MeOH, 5:1 (v/v)] containing deuterated internal standards. The samples were incubated for 1 h at -40°C to precipitate proteins and then centrifuged at 900×g for 15 min at 4°C. The supernatant was removed and evaporated to dryness. Dry extracts were reconstituted in 100 μl of DCM:MeOH (1:1, v/v) and then centrifuged at 13,800×g. A total of 75 μl of supernatant was collected for analysis.
"],"organism":["African Green Monkey"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14970"],"author":["hanchi miao. Institute of biophysics, Chinese academy of sciences. Datun Road No.15, Chaoyang, Beijing. hc_miao@ibp.ac.cn."],"data_transformation_protocol":["The raw data files were converted to files in mzXML format using the ‘msconvert’
program from ProteoWizard. The CentWave algorithm in XCMS was used for for peak
detection, extraction, alignment, and integration, the minfrac for annotation was set at
0.5, the cutoff for annotation was set at 0.3.
"],"study_factor":["Design"],"submitter_email":["hc_miao@ibp.ac.cn"],"sample_collection_protocol":["Cell were collected as pellets and stored in -80 degree until lipid extraction.
"],"omics_type":["Metabolomics"],"study_design":["Thermo Scientific Vanquish UHPLC System","kidney","POSITIVE","Negative","hereditary spastic paraplegia","untargeted analysis","African Green Monkey","Thermo Scientific Orbitrap Exploris 120","neurodegeneration","Lipidomics","experimental blank","lipid"],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","kidney","POSITIVE","hereditary spastic paraplegia","Negative","untargeted analysis","Thermo Scientific Orbitrap Exploris 120","African Green Monkey","neurodegeneration","Lipidomics","experimental blank","lipid"],"mass_spectrometry_protocol":["Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS
spectra in the control of the acquisition software (Xcalibur, Thermo). In this mode, the
acquisition software continuously evaluates the full scan MS spectrum. The ESI source
conditions were set as following: sheath gas flow rate as 30 Arb, Aux gas flow rate as
10 Arb, capillary temperature 320 ℃, full MS resolution as 60000, MS/MS resolution as
15000, collision energy: SNCE 15/30/45, spray voltage as 3.8 kV (positive) or -3.4 kV
(negative), respectively.
"],"additional_accession":[]},"is_claimable":false,"name":"ER fusogens maintain membrane reservoir to ensure brain function","description":"How the morphological dynamics of the endoplasmic reticulum (ER) are linked to its functions is unclear. Atlastins (ATLs), a class of GTPases, mediate ER fusion, and human mutations in ATL1 cause hereditary spastic paraplegia (HSP). Here, we show that ATL2-knockout mice are embryonic lethal with compromised development, particularly of the cerebellum. ATL2 is highly expressed in neuroglia, though ATL1 is dominant in the brain. Lack of ATL2 disorganizes the positioning of Bergmann glia, which in turn interferes with granule cell migration. These cells have significant shrinkage in the intracellular membrane area, which is associated with decreased phosphatidylcholine and cholesterol synthesis. When tested in calyx-type synapses in ATL-deleted mice, a reduced membrane reservoir, represented by fewer presynaptic vesicles, leads to defective synaptic function and hearing impairment. Collectively, these findings suggest that ER-shaping activity by ATL is essential for sustained lipid synthesis, and boosted lipid uptake is potentially beneficial for HSP patients.","dates":{"publication":"2026-07-08","submission":"2026-07-08"},"accession":"MTBLS14970","cross_references":{}}