The raw data collected using MassLynx V4.2 is processed by Progenesis QI software for peak extraction, peak alignment and other data processing operations, based on the Progenesis QI software online METLIN database and Biomark’s self-built library for identification.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["The column used is purchased from Waters Acquity UPLC HSS T3 column (1.8um 2.1*100mm). Positive ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile. Negative ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile. Injection volume 2μL.
"],"publication":["Gill Metabolomics of Sinopotamon planum under Acute Salinity Stress."],"submitter_name":["Aobo Pang"],"submitter_affiliation":["Yancheng Teachers University"],"organism_part":["Gill"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Frozen gill samples were homogenized in ice cold methanol, centrifuged, and the supernatant was dried and reconstituted in methanol/water before filtration and HPLC injection. Mixed extract from all samples, injected regularly to monitor instrument stability.
"],"organism":["Longpotamon planum"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14982"],"author":["Aobo Pang. Yancheng Teachers University. public_jlcbe@163.com."],"data_transformation_protocol":["The raw data collected using MassLynx V4.2 is processed by Progenesis QI software for peak extraction, peak alignment and other data processing operations, based on the Progenesis QI software online METLIN database and Biomark’s self-built library for identification.
"],"study_factor":["Salinity stress"],"submitter_email":["public_jlcbe@163.com"],"sample_collection_protocol":["We treated Sinopotamon planum with seawater of different salinities (0 ppt, 15 ppt, 30 ppt). Gills were collected for metabolomic analysis 96 hours after exposure.
"],"omics_type":["Metabolomics"],"study_design":["pooled quality control sample","Metabolomics","Waters ACQUITY UPLC I-Class PLUS System","Gill","Waters Xevo G2-XS QTof","untargeted analysis","MassLynx","Waters (China)","Progenesis QI","Longpotamon planum","experimental sample"],"curator_keywords":["pooled quality control sample","Metabolomics","Gill","Waters ACQUITY UPLC I-Class PLUS System","Waters Xevo G2-XS QTof","untargeted analysis","MassLynx","Waters (China)","Progenesis QI","Longpotamon planum","experimental sample"],"mass_spectrometry_protocol":["Waters Xevo G2-XS QTOF high resolution mass spectrometer can collect primary and secondary mass spectrometry data in MSe mode under the control of the acquisition software (MassLynx V4.2, Waters). In each data acquisition cycle, dual-channel data acquisition can be performed on both low collision energy and high collision energy at the same time. The low collision energy is off, the high collision energy range is 10~40V, and the scanning frequency is 0.2 seconds for a mass spectrum. The parameters of the ESI ion source are as follows: Capillary voltage: 2500V (positive ion mode) or -2000V (negative ion mode); cone voltage: 30V; ion source temperature: 100°C; desolvent gas temperature 500°C; backflush gas flow rate: 50L/ h; Desolventizing gas flow rate: 800L/h.
"],"additional_accession":[]},"is_claimable":false,"name":"Gill Metabolomics of Sinopotamon planum under Acute Salinity Stress","description":"This study investigated the metabolomic responses in the gills of the freshwater crab Sinopotamon planum after acute exposure to three salinity levels. Individuals were exposed to 0 ppt (freshwater control), 15 ppt, and 30 ppt for 96 hours, after which gill tissues were dissected and subjected to metabolomic analysis.","dates":{"publication":"2026-07-09","submission":"2026-07-09"},"accession":"MTBLS14982","cross_references":{}}