MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/m_MTBLS15037_LC-MS_alternating_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/s_MTBLS15037.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/a_MTBLS15037_LC-MS_alternating_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-9.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-8.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-7.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-8.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-9.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-9.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/A-3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-8.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-10.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/QC-5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/D-4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037/FILES/RAW_FILES/C-6.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15037<p>The resulting dataset underwent metabolite identification and preprocessing, after which data quality was assessed prior to further statistical analysis.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - hilic<p>Samples were separated using a Vanquish ultra-high performance liquid chromatography (UHPLC) system with a HILIC column. The column temperature was maintained at 25 °C, the flow rate was 0.3 mL/min, and the injection volume was 2 μL. The mobile phase consisted of solvent A (water containing 25 mM ammonium acetate and 25 mM ammonia solution) and solvent B (acetonitrile).</p><p>The gradient elution program was set as follows: 0–1.5 min, 98% B; 1.5–12 min, linear decrease from 98% to 2% B; 12–14 min, maintained at 2% B; 14–14.1 min, linear increase from 2% to 98% B; 14.1–17 min, maintained at 98% B. During the entire analysis, all samples were stored in an autosampler at 4 °C.</p><p>To minimize systematic signal fluctuations, all samples were analyzed continuously in a randomized order. Quality control (QC) samples were interspersed throughout the sample sequence to monitor instrument stability and ensure the reliability of analytical data.</p>Ginsenoside Rg5, a novel activator of arginine metabolic reprogramming, protects against hypoxic neuronal injury and cognitive deficits via dual-targeting ASL and ODC1.Changchun University of Chinese MedicineTingting LouCellmass spectrometry assay<p>Samples were thawed slowly at 4 °C. An appropriate amount of each sample was mixed with pre-cooled methanol/acetonitrile/water solution (2:2:1, v/v), followed by vortex mixing and low-temperature sonication for 30 min. The mixture was stood at −20 °C for 10 min and then centrifuged at 14000 g for 20 min at 4 °C. The supernatant was collected and vacuum-dried.</p><p>Prior to mass spectrometry analysis, the dried residue was reconstituted in 100 μL acetonitrile aqueous solution (acetonitrile: water = 1:1, v/v), vortexed, and centrifuged at 14000 g for 15 min at 4 °C. The resulting supernatant was subjected to injection and analysis.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS15037Tingting Lou. Changchun University of Chinese Medicine. ltt19900811@163.com.<p>Raw data were converted to .mzXML format via ProteoWizard, followed by peak alignment, retention time correction, and peak area integration using XCMS software. </p>Groupltt19900811@163.com<p>After seeding neural cells onto a culture plate, Rg5 pretreatment was performed and placed in an anaerobic environment.</p>MetabolomicsMetabolomicshypoxiaMultiQuantThermo Scientific Q Exactiveuntargeted analysisHomo sapienscellGinsenoside Rg5Vanquish LCMetabolomicshypoxiaMultiQuantThermo Scientific Q Exactiveuntargeted analysisHomo sapiensGinsenoside Rg5cellVanquish LC<p>Samples were separated using a Vanquish LC ultra-high-performance liquid chromatography (UHPLC) system and then analyzed on a Q Exactive series mass spectrometer (Thermo Fisher Scientific) in both positive and negative electrospray ionization (ESI) modes.</p><p>The ESI source and MS parameters were set as follows: nebulizer gas (Gas1): 60; auxiliary heating gas (Gas2): 60; curtain gas (CUR): 30 psi; ion source temperature: 600 °C; ion spray voltage (ISVF): ±5500 V (for positive and negative modes). For MS1 full-scan acquisition, the mass-to-charge ratio range was 80–1200 Da, with a resolution of 60 000 and an accumulation time of 100 ms. For MS2, a segmented acquisition method was employed, with a scan range of 70–1200 Da, a resolution of 30 000, an accumulation time of 50 ms, and a dynamic exclusion time of 4 s.</p>PhenylpyruvateCorticosteroneSalmeterol2(1h)-pyridinoneMilnacipranPyrocatechol(2-{[3-hydroxy-2-tetradecanamidooctadec-4-en-1-yl phosphonato]oxy}ethyl)trimethylazaniumN-(octadecanoyl)sphing-4-enine-1-phosphocholine1-o-octadecyl-sn-glyceryl-3-phosphorylcholineStearoyl ethanolamidePantothenolLuteinizing hormone releasing hormone humanAdenosine 5'-triphosphate (atp)KetoleucineReserpineUridine 5'-triphosphateIle-thrIminodiacetic acid(-)-quebrachitolDL-prolineValdecoxibGlycolateD-erythro-sphingosine-1-phosphateButyl 4-hydroxybenzoateCapric acidCis-5-dodecenoic acidDihydroergotamineDl-cystathionineCyclohexylamineThymidine 5'-triphosphatePilocarpineTetrahydrofolateMaltitolMevalonic acidArginine21-hydroxyprogesteroneSecobarbitalD-ribose 1-phosphateGlutathioneHydroferulic acid2-hydroxyisocaproic acidLeucylleucine4-hydroxy-3-methoxybenzyl alcohol1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n,n-dimethylL-carnitineL-theanineFexofenadine.beta.-naphtholTemazepamPc 36:2OleamideMethylergonovineKainic acidPantothenic acidEtodolacNicotinic acid adenine dinucleotide (NAAD)RitonavirSalicylaldehyde1-oleoyl-2-stearoyl-sn-glycero-3-phosphocholineErucic acidN-phenyl-2-naphthylamineSaquinavirPipemidic acidAzelastineAflatoxin b1Vanillic acidSuccinic semialdehydeOctaethylene glycol1,2-dioleoyl-sn-glycero-3-phosphatidylcholineGln-proBenzyl alcohol2-hydroxy-5-methoxybenzoic acidL-threonatePyridineAla-AlaTrans-traumatic acidDigitoxin1-(1z-octadecenyl)-2-(5z,8z,11z,14z-eicosatetraenoyl)-sn-glycero-3-phosphocholineCyprodinil5-aminovaleric acidAndrosterone glucuronide4-hydroxytamoxifenIndoleFusidic acidD-ornithineIsocitric acidN,n-dimethylguanosineThiopentalLeupeptinCyclopentolateGdp-l-fucoseAdenosineEstrone sulfateN-propyl gallate4-imidazoleacrylic acidPterostilbeneBenazepril2-o-methyladenosineDL-threonineMelatonin(-)-secoisolariciresinolTrazodonaIsoproterenol3,3-dimethylglutaric acid6-hydroxymelatoninD-glucono-1,5-lactone4-piperidinecarboxamidePC(16:0/16:0)LovatatinSphingosineN-Acetyl-L-tyrosine1-(1z-octadecenyl)-2-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glycero-3-phosphocholine5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (aicar)Tricin methyl etherTelmisartanPsychosineLysineAcetaminophenVildagliptinCabergolinePhenylacetaldehydeHexobarbitalClindamycinRosuvastatin4-vinylphenolGuanineMeperidineSuberoylanilide hydroxamic acid4-(4-chlorophenyl)-4-hydroxypiperidineNADHS-methyl-5'-thioadenosineUdp-n-acetylglucosamine2-amino-1-phenylethanolCytidine 5'-monophosphate (CMP)L-propionylcarnitineFlunisolideMyo-inositolAtrazine desisopropylN-hydroxy-4-aminobiphenyl1-methyladenosineXanthosine.beta.-ecdysoneAll-trans-4-hydroxyretinoic acidUridine 5'-monophosphatePitavastatinNorcodeineTris(hydroxymethyl)aminomethaneNAD+Atrazine1,2-dioleoyl-sn-glycero-3-phospho-l-serineN-acetylputrescineGln-hisMilrinone2-hydroxybenzyl alcoholGlycocholic acidPhytic acidOctadecanoic acidGlycocholateRanolazineChenodeoxycholate1-(1z-octadecenyl)-sn-glycero-3-phosphocholineBisphenol aIndole-3-carboxaldehydeWarfarinHomoharringtonineAdrenosteroneOxyacanthineDL-isoleucine(3,4-dihydroxyphenyl)ethanolHydroquinoneO-acetyl-l-serinePro-ThrDeoxythymidine 5'-phosphate (dTMP)Guanidinopropionic acidGuanidinoethyl sulfonateAsiatic acidAdenosine 5'-phosphosulfateTaurochenodeoxycholateD-prolinePyrimethanilTiagabineTestosterone enanthateLignoceric acidAllopurinol ribosideNabiloneHis-LysHis-serLithocholylglycineThymineDobutamine9-phenanthrolBiocytinOfloxacinPamidronic acid7-methylguanineTrifluoroperazineTroloxCyclic gmpSarcosineTaurineZymosterolAlanopineProbucolNaltrexone(3r,5s,7r,9s,10s,12s,13r,14s,17r)-17-((2r)-7-hydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triolNelfinavirEprosartan4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acidAgmatineIsoliquiritigeninPyridoxalGlycerolDanazol(-)-atropineL-aspartic acid5-L-Glutamyl-L-alanine2-linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamine5.alpha.-androstan-17.beta.-ol-3-one sulfateLoperamideTopiramateUndecanoic acidPyrrole-2-carboxylic acidFructose 1-phosphate17alpha-hydroxyprogesteroneQuinaldic acid1,2-dioleoyl-sn-glycero-3-phosphateOrlistatTriethanolamine3-dehydroepiandrosterone sulfateGeranylgeranyl pyrophosphateInosine 5'-monophosphateN-acetylcadaverine.beta.-cyano-l-alanine1-Stearoyl-2-oleoyl-sn-glycerol 3-phosphocholine (SOPC)Oleoyl-l-carnitineSecoisolariciresinolOlopatadine1-stearoyl-2-docosahexaenoyl-sn-glycerolDopamineTamoxifenMenaquinone 4N-acetylaspartylglutamic acidButalbital.alpha.-L-Glu-L-AspPantothenateTestosteroneCyclohexanesulfamic acidPsoralidinDl-a-hydroxybutyric acidDofetilideSarafloxacinUracilAmprenavirEicosapentaenoic acidRheinQuinaprilAzithromycin2-hydroxyestradiolMorphineDiflunisalAllyl isothiocyanateCreatineCytidine 5'-monophosphate.beta.-nicotinamide adenine dinucleotide(NAD)CytosinePhenaceturic acidFenbendazoleFurostane base -2h + 1o, o-hex, o-pen-dhexCis-4,7,10,13,16,19-docosahexaenoic acidBisoprololMalatePregnenolone sulfateFlusilazoleAsp-Arg3-Phospho-D-glycerateMontelukast sulfoxideAdipic acidDl-Leu-dl-ValAdenosine 5'-diphosphateChloroquineLomefloxacin1-stearoyl-2-linoleoyl-sn-glycero-3-phospho-l-serineFolate.alpha.-d-galactose 1-phosphateApigenin 7-glucoside1,2-dipalmitoleoyl-sn-glycero-3-phosphocholineDisopyramideIndinavirN-acetyl-.beta.-d-mannosamineIsoleucine6-hydroxynicotinic acidThiouric acidCilastatinPyruvateN2-Acetyl-L-ornithine(3r,7r,9s,10s,12s,13r,14s,17r)-17-((2r)-6,7-dihydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triolCdp-ethanolaminePro-TrpDL-Glutamic acidCaproic acidN-acetyl-d-lactosamineDihydroxyacetone phosphate(+)-chlorpheniramineFamciclovirPalmitic acidChitobioseHistidineLnt15(s)-hydroxy-(5z,8z,11z,13e)-eicosatetraenoic acid1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholineD-aspartic acidStearamidePhosphoric acidNervonic acidPropionic acid7,8-dihydroneopterinPhenylbutazoneDeoxyadenosineOseltamivirPrazosinPergolideDillapiolEpigallocatechin gallateGatifloxacinN-Acetyl-D-Glucosamine 6-PhosphateGlycerophosphocholine3-methyladenineActinomycin dGlimepiride1-methylhydantoinAmikacinTripropylamine1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholineDipivefrine1-pentadecanoyl-sn-glycero-3-phosphocholine2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip)Medicagenic acid base + o-hexDiethylcarbamazineHydroxyphenyllactic acid.beta.-glycerophosphateSalicylic acid11alpha-hydroxyprogesteroneXanthineEthyl lauratePramoxineKetamineCholineAsparagineL-gulono-1,4-lactone2',3'-dideoxycytidineFluorometholoneAcetylcysteineD-arabinonic acid.gamma.-glu-cysNandroloneMaleic acidLsdSulfentrazoneN.epsilon.-acetyl-l-lysineCrystal violetHydrocodoneIndoxyl sulfateDocetaxelL-pipecolic acid2-arachidonoyl-1-stearoyl-sn-glycero-3-phosphoethanolaminePhosphorylcholineIndole-3-pyruvic acid1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholineTrp-CysPhenobarbitalFluphenazineIrbesartanN-acetyl-d-glucosamineQuinidineDihydroisoferulic acidDimethyl sulfoxidePyruvaldehydeD-arabitolSarsasapogeninDesferrioxamineSulfadiazineHis-TyrVitamin k15,6,7,3',4'-pentamethoxyflavoneLeucine2-hydroxymyristic acid1-methyl-l-histidineDihydrothymine1h-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4h,12h)-dione, 4,11-diethyl-4,9-dihydroxy-, (4s)-MifepristoneCurcuminPiperidine4-androstene-11.beta.,17.beta.-diol-3-onePolydatinAtazanavirgamma-L-Glutamyl-L-phenylalanineProstaglandin f2.beta.ZiprasidoneN-lauroyl-d-erythro-sphingosylphosphorylcholine6-HydroxydopamineArecaidineDethiobiotin10-nitrooleic acidPhosphocholinePyridoxal phosphateXylitolLactoseAlbendazoleDocosatetraenoyl ethanolamideTaurolithocholic acid sulfateVitamin cMandelonitrileN-acetyl-l-phenylalanine4-androsten-11.beta.-ol-3,17-dioneNorbuprenorphineGln-AlaIsoeugenyl acetate(-)-.alpha.-kainic acidD-myo-inositol-1,3-diphosphateAcenapthyleneDoxazosinL-thyronineIndicanEthylenediaminetetraacetic acidOxyresveratrolProstaglandin f1.alpha.Coniferyl alcoholDeoxyinosineCortisol 21-sulfateDiazepamMangostineProstaglandin i2FlavoneNicotinic acid adenine dinucleotideBiotinPaxillineRac-2-despiperidyl-2-aminorepaglinidePhenolDL-tryptophanL-glutathione, reducedNeomycinAmlodipineMesaconic acidMethylphenidateD-galacturonic acidKukoamine aTetraethylene glycol monomethyl etherL-methionineAdenylosuccinateGlycineIbuprofen .beta.-d-glucuronideGln-met1-arachidoyl-2-hydroxy-sn-glycero-3-phosphocholineGlu-ArgOleic acidN-acetyl-l-aspartic acidEstriolPropafenoneCis,cis-muconic acidFructose 1,6-diphosphateDecanoyl-l-carnitinePseudouridineIminostilbenePentazocineRotenone1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamineL-homoarginine6-phosphogluconic acidIsoetharineN-oleoylethanolamineBetulinic acid2'-o-methyladenosineMycophenolate mofetilCiprofloxacinSoyasapogenol b + dhex + hex + hexa + malonyl5-aminolevulinic acidPropofol .beta.-d-glucuronide2'-deoxyuridine 5'-monophosphateIsovaleric acid4-pyridoxic acidSinapyl alcoholBisdemethoxycurcuminProstaglandin d1CitrateDoxorubicinGlyceryl tripalmitoleateKetoprofenGlycyrrhizic acidOctanoylcarnitine1,2-ditetradecanoyl-sn-glycero-3-phosphocholineG-guanidinobutyrateGlyceric acidDL-cysteineN-acetyl-l-glutamateImatinibBiliverdinN8-acetylspermidineProstaglandin e3Arachidonic acid (peroxide free)L-norvalinePalmitoyl sphingomyelinCis-11-eicosenoic acidHexose + c13h21o2L-iditolUridine2-oxooctanoic acidRaloxifeneGlu-PheEnterodiolThiosulfuric acid1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholineN6-methyl-l-lysineCoproporphyrin iDulcitolIsoxanthopterinN-myristoylsphinganineN-oleoyl-d-erythro-sphingosylphosphorylcholineBeclomethasoneLosartanPyroquilon15-ketoprostaglandin f2.alpha.Thiamine(.+/-.)-11-nor-.delta.9-tetrahydrocannabinol-9-carboxylic acidUdp-d-glucoseL-homoserine.gamma.-aminobutyric acidZafirlukastDesmethylxanthohumolHomogentisic acidNor-nitrogen mustard3,4-dihydroxymandelic acidArgininosuccinic acidHypotaurine3,4-dihydroxy-l-phenylalanineLevofloxacinN-acetyl-l-methionineTrihexyphenidylTrachelanthineGamma-glutamylvalineN-glycolylneuraminic acidPhlorizinDiphenoxylatePhloretin(-)-riboflavin3-hydroxymyristic acidPhe-proLopinavirNorfloxacin1-(1z-hexadecenyl)-sn-glycero-3-phosphocholineCinnavalininateAmoxicillinD-erythrose 4-phosphatePerindoprilTrimethylamine n-oxideGlutamic acid4-phenylbutyric acidSuberic acid5,6-dihydroxyindole-2-carboxylic acidOxaprozinTetraethylene glycolPropanoic acid1-palmitoyl-2-oleoyl-sn-glycerolPrunasin2-propylglutaric acidAlprenololCreatinine.alpha.-iononeNoscapinePyridoxine1-aminocyclopropanecarboxylic acidDiacetyl.beta.-nicotinamide adenine dinucleotide phosphateTrichlormethiazideCyproheptadineD-turanoseQuercitrinValineStachydrine.gamma.-undecalactoneGibberellic acidArachidonoyl ethanolamidePalmitoleoyl ethanolamideHexaethylene glycol4-hydroxyphenethyl alcoholD-2-phosphoglyceric acidAstaxanthinGuanosine diphosphate mannoseNicotinamide ribotideTenofovir4-aminophenol1,2-diarachidonoyl-sn-glycero-3-phosphocholineBetaineMitoxantroneJasmonic acidNortriptylineHesperetinUridine diphosphate glucose(UDP-D-Glucose)AtovaquoneKanamycin a5-hydroxy-l-tryptophanCytidineEnrofloxacin3-methylxanthine3-methylglutarylcarnitineGlu-AsnAtorvastatin.gamma.-dodecalactoneO-desmethylastemizoleMorphine-3-glucuronidePhosphoserineN-acetylneuraminic acidEthylmorphineAlfuzosinImazamethabenz-methylAlfentanyl4-methylbenzyl alcohol1-(3-pyridyl)-1-butanone-4-carboxylic acidCaffeate1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n-methylGenipinUridine 5'-diphosphateD-mannose 6-phosphatePalmitamidePhytolaccasaponin GSulindac1-myristoyl-2-hydroxy-sn-glycero-3-phosphoethanolamineStrychnineRamiprilatS-carboxymethyl-l-cysteineTrioxsalenGlutathione, oxidizedL-pyroglutamic acidPhendimetrazineGuanosine 5'-monophosphate1-o-hexadecyl-2-o-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glyceryl-3-phosphorylcholineVincristineCodeineQuinateFluocinolone acetonide19-hydroxyandrost-4-ene-3,17-dioneButylated hydroxytoluene2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholineProgesteroneFlecainideS-allyl-l-cysteineNaringeninAllantoinDapsoneD-amygdalin11beta-hydroxyprogesteroneEnalaprilGamma-l-glutamyl-l-glutamic acidPiperacillinAtropinePhosphoenolpyruvateAdenosine 3'-monophosphateArctigeninD-SorbitolCytidine 5'-diphosphocholineLisinoprilThiamine monophosphateSuccinateEsmolol5'-deoxyadenosineGly-LeuNg,ng-dimethyl-l-arginine(2-aminoethoxy)[3-[hexadec-1-en-1-yloxy]-2-[icosa-5.8.11.14-tetraenoyloxy]propoxy]phosphinic acidPentamidinePentostatinLinezolidCholesteryl sulfate12-hydroxydodecanoic acidRicinoleic acidMethoxsalenCitrininL-citrullineL-cystinePapaverinePentadecanoic acidShikimateDL-valine6-ketoprostaglandin e1NiacinamideCis-13-eicosenoic acidCoenzyme q2L-fucose-1-phosphateL-threonineSerotoninKojic acid7.alpha.-hydroxy-4-cholesten-3-oneLeukotriene d4Sanguinarine4-hydroxyvalsartanGuanosine 5'-monophosphate (GMP)Estrone glucuronideFlutamideFumarateLeukotriene c4(+)-catechin(2-aminoethoxy)[2-[docosa-4.7.10.13.16.19-hexaenoyloxy]-3-[octadeca-1.9-dien-1-yloxy]propoxy]phosphinic acidHexylamineIndolelactic acidAcetolTaurocholate3'-o-methylguanosineMaltotetraoseHarmineStreptomycin aDL-asparagineD-asparaginePhytosphingosinePro-hypFormoterolPropranololIsopentenyl pyrophosphateTyrosine4-hydroxybenzaldehyde4-hydroperoxy-2-nonenalO-phosphotyrosineLevamisoleQuinineCoumarin1-palmitoyl-sn-glycero-3-phosphocholineMyristic acidSepiapterinHypoxanthineBergaptolPhenyllactic acidLinamarinAcetyl-l-carnitineCamp16.alpha.-hydroxyestroneL-Arginine1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamineD-glutamineNevirapineAndrostan-3-ol-17-one 3-glucuronide(S)-2-HydroxyglutaratePutrescine3-hydroxyphenylacetic acid.gamma.-hydroxybutyric acidL-homocitrulline1,2-dioleoyl-sn-glycerolShikonin1,2-dihexadecanoyl-sn-glycero-3-phosphocholinePhenylacetyl-l-glutamineNicotinate d-ribonucleotideTropineCaptoprilAdenineEnoxacinS-adenosyl-l-methionine2-arachidonoylglycerolCortisone1-Stearoyl-sn-glycerol 3-phosphocholine(LPC(18:0))2,4-dichlorophenolEnalaprilatInosinePurineCocaineDL-tyrosineEbastineQuinacrineRac-n,n-didesmethyl-o-desmethylvenlafaxine glucuronidePrimaquine7-ethyl-10-(4-n-aminopentanoic acid)-1-piperidino)carbonyloxycamptothecinGartaninCholic acidCarnosolCytidine monophosphate n-acetylneuraminic acid2'-Deoxyinosine 5'-monophosphatePhloroglucinocarboxaldehydeCaffeineD-gluconateO,o-dimethyl dithiophosphateNonanoic acidDiltiazem1,2-dioleoyl-sn-glycero-3-phospho-(1'-myo-inositol)Isobutyryl-l-carnitine4-methyl-1h-pyrazoleDihydrouracilRanitidineHomocitrate4,2'-dihydroxy-4',6'-dimethoxychalconeAnacardic acid1-myristoyl-sn-glycero-3-phosphocholinePicolinic acidS-adenosyl-l-homocysteinePrephenateTridemorphGlucose 1-phosphate3-aminohexanoic acidPerphenazine4-hydroxycyclophosphamide1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholineNisoldipineDodecanedioic acidS-AdenosylmethionineL-palmitoylcarnitine3-methylthiopropionateDibutyl phthalateLabetalol3-Butynoic acidSofalconeD-2-aminobutyric acidD-neopterin5-hydroxyindoleacetateIndole-3-acetamide(+)-isocorydineTramadol1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholineValganciclovirLtc4-[d5]5-methoxytryptophanL-hydroxyarginineN,n-dimethylformamideHistamineMethyltyrosinateEplerenoneAminophenazoneNoroxymorphoneProtoporphyrin ixD-glucarate17.beta.-nandrolone decanoateMitragynineN,n,n-trimethyllysineOxycodoneN-methyl-l-glutamic acid3-methyl-l-histidinePuerarin16-hydroxyhexadecanoic acidTaurodeoxycholic acidTridecanoic acid (Tridecylic acid)CarboxyphosphamideL-Carnosine1-docosahexaenoyl-2-stearoyl-sn-glycero-3-phosphocholine1-heptadecanoyl-sn-glycero-3-phosphocholinePyrrolidineL-arabinoseQuinolinateEpinephrineDL-serineVindesineNicotinateTerfenadineD-erythronic acid .gamma.-lactonePhosphocreatineAcebutololClozapineQuercetin 3'-methyl etherN-acetylhistidineTobramycinOndansetronfalseGinsenoside Rg5 protects against hypoxic neuronal injuryFor metabolome analysis, major metabolic intermediates were analyzed with technical support from Applied Protein Technology Co., Ltd. (Shanghai, China) 17. Briefly, eight treated and untreated cell samples stored in liquid nitrogen were processed and separated by ultrahigh-performance liquid chromatography (UPLC) (1290 Infinity LC system; Agilent Technologies), followed by detection with a Triple Quad 6600 mass spectrometer (QTRAP/MS; AB SCIEX, Framingham, MA, USA).Energy metabolism-related metabolites were identified using Multiquant software.2026-07-142026-07-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