MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/m_MTBLS15043_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/a_MTBLS15043_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/s_MTBLS15043.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/DCM1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/CT2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/DCM3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/CT1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/DCM5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/CT4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/DCM2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/CT3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/CT5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043/FILES/RAW_FILES/DCM4.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15043<p>After sum-normalization, the processed data were analyzed by R package (ropls), where it was subjected tomultivariate data analysis, including Pareto-scaled principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). The 7-fold cross-validation and response permutation testing were used toevaluate the robustness of the model. The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Student’s t test was applied to determine thesignificance of differences between two groups of independent samples. VIP &gt; 1 and p value &lt; 0.05 were used to screensignificant changed metabolites. Pearson’s correlation analysis was performed to determine the correlation between twovariables.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p>Samples were separated on an Agilent 1290 Infinity LC ultra-high performance liquid chromatography (UHPLC) system equipped with a HILIC column. The column temperature was maintained at 25 °C with a flow rate of 0.5 mL/min and an injection volume of 2 μL. Mobile phase A consisted of water supplemented with 25 mM ammonium acetate and 25 mM ammonia water, while mobile phase B was pure acetonitrile. The gradient elution procedure was set as follows: 0–0.5 min, held at 95% B; 0.5–7 min, linear decrease of B from 95% to 65%; 7–8 min, linear decrease of B from 65% to 40%; 8–9 min, held at 40% B; 9–9.1 min, linear increase of B from 40% to 95%; 9.1–12 min, held at 95% B. All samples were kept at 4 °C in the autosampler throughout the analytical run. To eliminate signal fluctuation bias, all samples were analyzed in a randomized continuous sequence. Quality control (QC) samples were interspersed within the sample queue to monitor instrumental stability and evaluate the reliability of experimental data.</p>Untargeted Metabolomic Profiling of Left Ventricular Myocardium in a Mouse Model of Diabetic Cardiomyopathy.Tianjin First Central Hospitalfeng zhangHeartmass spectrometry assay<p>The tissue samples with 200 μL of H2O andfive ceramicbeads were homogenized using the homogenizer. 800 μL methanol / acetonitrile(1: 1, v / v) were added tohomogenizedsolution for metabolite extraction.The mixture was centrifuged for 20 min(14000 g, 4 °C).Thesupernatant was dried in avacuum centrifuge.For LC - MS analysis, the samples were re - dissolved in 100 μLacetonitrile / water(1: 1, v / v) solventand centrifuged at 14000 g at 4 ℃ for 15 min, then the supernatant wasinjected.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS15043feng zhang. Tianjin First Central Hospital. fengzhang0222@163.com.zhuqing li. Tianjin First Central Hospital. dinger326@126.com.<p>The raw MS data were converted to MzXML files using ProteoWizard MSConvert before importing into freelyavailable XCMS software. For peak picking, the following parameters were used: centWave m/z = 10 ppm, peakwidth = c(10, 60), prefilter = c (10, 100). For peak grouping, bw = 5, mzwid = 0.025, minfrac = 0.5 were used. CAMERA (Collection ofAlgorithms of MEtabolite pRofile Annotation) was sued for annotation of isotopes and adducts. In the extracted ionfeatures, only the variables having more than 50% of the nonzero measurement values in at least one group were kept.Compound identification of metabolites was performed by comparing of accuracy m/z value (&lt;10 ppm), and MS/MSspectra with an in-house database established with available authentic standards.</p>Treatmentfengzhang0222@163.com<p>The animal tissues were quickly frozen in liquid nitrogen immediately after dissection. Thenthetissue were cut on dry ice(~80 mg) into an Eppendorf tube(2 mL).The tissue samples with 200 μL of H2O andfive ceramicbeads were homogenized using the homogenizer. 800 μL methanol / acetonitrile(1: 1, v / v) were added tohomogenizedsolution for metabolite extraction.The mixture was centrifuged for 20 min(14000 g, 4 °C).Thesupernatant was dried in avacuum centrifuge.For LC - MS analysis, the samples were re - dissolved in 100 μLacetonitrile / water(1: 1, v / v) solventand centrifuged at 14000 g at 4 ℃ for 15 min, then the supernatant wasinjected.</p>MetabolomicsHeartMetabolomicsMus musculusThermo Scientific Orbitrap Exploris 240untargeted analysisexperimental blankdiabetic cardiomyopathyAgilent 1290 Infinity LCuntargeted metabolite profilingHeartMetabolomicsMus musculusThermo Scientific Orbitrap Exploris 240untargeted analysisexperimental blankdiabetic cardiomyopathyAgilent 1290 Infinity LCuntargeted metabolite profiling<p>Primary and secondary mass spectra were acquired using an AB Triple TOF 6600 mass spectrometer. After chromatographic separation by the Agilent 1290 Infinity LC UHPLC system, mass spectrometric detection was performed on the AB SCIEX Triple TOF 6600 instrument under electrospray ionization (ESI) positive and negative modes separately. ESI source parameters were configured as follows: nebulizer gas (Gas 1): 60, heater gas (Gas 2): 60, curtain gas (CUR): 30 psi, ion source temperature: 600 °C, ion spray voltage (ISVF): ±5500 V for positive and negative modes. The m/z scanning range for MS1 was set at 60–1000 Da, and the m/z range for MS2 fragment ions was 25–1000 Da. The accumulation time was 0.20 s per spectrum for MS1 scans and 0.05 s per spectrum for MS2 scans. Data-dependent acquisition (IDA) was applied to collect MS2 spectra based on peak intensity screening. Declustering potential (DP) was set to ±60 V for both ionization modes, and collision energy was 35 ± 15 eV. IDA parameters were set as follows: the dynamic exclusion range of isotopic ions was 4 Da, and a maximum of 10 fragment spectra were triggered per full scan.</p>Carfentrazone-ethylAcenapthyleneSchaftosideL-bunolol2(1h)-pyridinoneSolanidine1-palmitoylglycerolN-(octadecanoyl)sphing-4-enine-1-phosphocholineLauramidopropylbetaine3-pyridinecarboxaldehydeDictamineStearoyl ethanolamideEctoineDibenz(a,h)acridinePantothenolMethapyrileneNormorphine5-methylbenzotriazole2-((4r)-4-((3r,5s,9s,10s,13r,14s,17r)-3-hydroxy-10,13-dimethyl-7-oxohexadecahydro-1h-cyclopenta[a]phenanthren-17-yl)pentanamido)ethane-1-sulfonic acidFlufenacetMethanone, [1-(5-fluoropentyl)-6-hydroxy-1h-indol-3-yl](2,2,3,3-tetramethylcyclopropyl)-Prostaglandin i2N-arachidonoyldopamineFlavoneAngeloylgomisin hTropinoneBiotinPhenolVincamoneSyringic acidAmpicillinNapellineMethylphenidateFumagillinD-galacturonic acidDicyclohexylamineN-acetyl-5-hydroxytryptamineSimeconazolePro-glnCyclohexylamineL-methionineDinotefuranMyristoyl-l-carnitineD-erythro-sphinganine-1-phosphate4-hydroxychalconeN-(.alpha.-linolenoyl)tyrosineArginine1-arachidoyl-2-hydroxy-sn-glycero-3-phosphocholine1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol)RicinineDicyclominePalmitoleoyl 3-carbacyclic phosphatidic acid3-phenylazopyridine-2,6-diamine(.+/-.)-cromakalimMelamine2-(2',3',4'-trihydroxybutyl)quinoxaline4-hydroxy-3-methoxybenzyl alcoholCinchonineL-carnitineFenpropidinTetrabenazine.beta.-naphthol1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamineAcetylvanillinN-lauroyl-d-erythro-sphinganineIsophoroneOleamideBetulinic acid2-aminobenzimidazoleHeliotrineAflatoxin g1N-acetyl-d-norleucineLevetiracetamDL-arginine15-deoxy-goyazensolideMetamitronTrimethoprimDoxorubicin4-aminophenyl-1-thio-.beta.-d-galactopyranoside4,7-dichloroquinolineProstaglandin e1 alcoholN,n-dimethylsphingosineOctanoylcarnitineBenzenemethanol, .alpha.-[(1r)-1-(methylamino)butyl]-, (.alpha.s)-rel-Aflatoxin b2Aflatoxin b1DL-cysteineOctaethylene glycol1,2-dioleoyl-sn-glycero-3-phosphatidylcholine11(Z),14(Z)-Eicosadienoic AcidGln-pro4,4'-diaminodiphenylmethaneN-3-oxohexadec-11z-enoyl-l-homoserine lactonePyraclonilSipeimineValine betaineL-FucoseBenzyl alcohol1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholineLinoleoylglycine5-methylisoxazol-3-amineAcetamide, 2-[4-[(5,6-diphenyl-2-pyrazinyl)(1-methylethyl)amino]butoxy]-n-(methylsulfonyl)-1-o-hexadecyl-2-o-(2e-butenoyl)-sn-glyceryl-3-phosphocholinePalmitoyl sphingomyelinFlavanone hydrazoneL-saccharopine1-(1z-octadecenyl)-2-(5z,8z,11z,14z-eicosatetraenoyl)-sn-glycero-3-phosphocholineCyproconazoleImazamox1,2-dilinoleoylglycerolCinoxacinN-nitrosopyrrolidineHis-HisTetrodotoxinTrans-zeatin1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine1-o-hexadecyl-2-o-(5z,8z,11z,14z,17z-eicosapentaenoyl)-sn-glyceryl-3-phosphorylcholine1-(1z-octadecenyl)-2-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine1,6-anhydro-.beta.-d-glucoseG0Met-Val-LysBenzamideIsopentenyladenineN-myristoylsphinganineN-oleoyl-d-erythro-sphingosylphosphorylcholineBeclomethasoneConiineN-docosanoyl-4-sphingenyl-1-o-phosphorylcholineOligomycin bTestosterone cypionateThr-AlaSwainsonineThiamine1,2-dilinoleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)Adenosine.gamma.-aminobutyric acidHuperzine b17-trifluoromethylphenyltrinorprostaglandin f2.alpha. methyl esterZafirlukastAndrost-5-en-17-one, 3-[2-(diethylamino)ethoxy]-, (3.beta.)-1-behenoyl-2-hydroxy-sn-glycero-3-phosphocholineCycloheximideAcetylcarnitine3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acidMyristoyl ethanolamideBaicaleinDehydroaripiprazoleArgininosuccinic acidDl-homocysteineMet-Met-ArgIsopimpinellinBenzamide, n-[2-[[(3r)-1-[trans-4-hydroxy-4-(6-methoxy-3-pyridinyl)cyclohexyl]-3-pyrrolidinyl]amino]-2-oxoethyl]-3-(trifluoromethyl)-5.alpha.-cholest-7-en-3.beta.-olMelatoninIrgarol3,5,9-trioxa-4-phosphatetracosan-1-aminium, 7-(acetyloxy)-24-carboxy-4-hydroxy-n,n,n-trimethyl-, inner salt, 4-oxide, (r)-Stearidonic acidLauric acid leelamideTrachelanthineIsoproterenol4-hydroxyquinoline2-(4-pyridyl)-4-methylthiazole-5-carboxylic acidXanomeline4-piperidinecarboxamide3-[(cholamidopropyl)dimethylammonio]-1-propanesulfonate1-Stearoyl-sn-glycerol 3-phosphocholineS-lactoylglutathioneSphingosineBenzoic acid, 3,6-dichloro-2-methoxy-1-methylhistidine1,2-benzenediamine, 4-methyl-n1-(3-phenylpropyl)-Leukotriene b4 dimethylamide.gamma.-muricholic acid2'-hydroxy-4'-methoxyacetophenone1h-indole-3-carboxylic acid, 1-(cyclohexylmethyl)-(-)-riboflavinBatimastat2-amino-2-methyl-1,3-propanediol1-(1z-octadecenyl)-2-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glycero-3-phosphocholineTetrandrineFingolimod5'-phosphoribosyl-5-amino-4-imidazolecarboxamide (aicar)1-(1z-hexadecenyl)-sn-glycero-3-phosphocholineDinex1-(1z-octadecenyl)-2-(5z,8z,11z,14z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamineLysineSclareolide5-methylcytosineLinoleoylcarnitinePerindoprilTrimethylamine n-oxide18.beta.-glycyrrhetinic acidAcadesine (drug)ScopolinCyclopeptineGibberellin a4SuberosinZearalenoneN-acetyldihydrosphingosineOxaprozin4-(methylamino)-4-(3-pyridyl)butyric acidBispyribacLauroyl-l-carnitineMeperidine2h-benzimidazol-2-one, 1-ethyl-1,3-dihydro-N,n-diethylcathinoneN-cyclohexylaniline1-monolinoleoyl-rac-glycerol1-palmitoyl-2-oleoyl-sn-glycerolN-(6-aminohexyl)-1-chloronaphthalene-5-sulfonamideS-methyl-5'-thioadenosineBenzimidazoleAlpha-d-glucose 1,6-bisphosphate5-methyl-5,6-dihydrouracilProsulfocarb2-methylamino-1-phenylbutane2-acetoxy-4-pentadecylbenzoic acid1-methyladenosineDodecanoic acid, 12-[[(cyclohexylamino)carbonyl]amino]-ProlintaneN-(2-aminoethyl)-5-isoquinolinesulfonamideN-.alpha.-acetyl-l-ornithineDiacetyl1,2-diamino-2-methylpropaneL-tryptophanamideRoseoflavinNorcodeineRottlerinStachydrine1-deoxy-1-(methylamino)-d-galactitolOleoyl ethylamide2-hydroxyestroneArachidonoyl ethanolamideZidovudinePalmitoleoyl ethanolamideHexaethylene glycolN-acetylglutamineLpc 18:1Lys-Cys1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-myo-inositol)BilirubinDecamethylcyclopentasiloxanePcb 52Phosmet-oxonGlycocholateChenodeoxycholate1-(1z-octadecenyl)-sn-glycero-3-phosphocholine3-(methylthio)-1-propanolBisphenol aGanciclovir1,2-diarachidonoyl-sn-glycero-3-phosphocholine2-cis-4-trans-abscisic acidSimetrynBetaine1,2-dioleoyl-sn-glycero-3-phospho-rac-1-glycerolD-erythro-imidazolylglycerol phosphateGomisin cN,n-diethyl-3,4-dimethoxybenzamidePiperettineN-arachidonoyl-.gamma.-aminobutyric acidHexamethylcyclotrisiloxane1-palmitoyl-2-lauroyl-sn-glycero-3-phosphorylcholine.alpha.-galactosyl-n-stearoylsphingosineEnrofloxacinZaleplonCarnitineHydroquinoneTaurohyocholic acidO-acetyl-l-serineWogonin1-hexadecyl-2-(8z,11z,14z-eicosatrienoyl)-sn-glycero-3-phosphocholineFipronil sulfoneN-hexadecanoyl-l-homoserine lactone4-quinolinecarboxylate3-amino-2,3-dihydrobenzoic acidAtorvastatinLpc 18:2TransfluthrinN-(tert-butyl)benzenesulfonamideTrilaurin4,5-epoxy-7z,10z,13z,16z,19z-docosapentaenoic acid, methyl esterCafestolAlfentanyl2-linoleoylglycerolAdenylosuccinic acid4-methylbenzyl alcoholCaffeateGenipinTiagabinePiperonyl sulfoxideRac-4-sulfoxypropranolol(-)-n6-(2-phenylisopropyl)adenosinePalmitamideZ-ligustilideTriptophenolide11,14,17-eicosatrienoic acid, (z,z,z)-TrioxsalenPhosmet6-bromoquinolin-2-olPymetrozinEmpenthrin1-linoleoyl-2-oleoyl-rac-glycerolLithocholylglycineD-ribose 5-phosphateGuanosine 5'-monophosphateNootkatone1-o-hexadecyl-2-o-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glyceryl-3-phosphorylcholine2-piperidinecarboxylic acid, 1-(3,3-dimethyl-1,2-dioxopentyl)-, (1r)-1-(3-aminophenyl)-3-(3,4-dimethoxyphenyl)propyl ester, (2s)-Sarcosine2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine2-epahsa [dmed-fahfa]Monomethyl glutaric acid7,8-dihydrobiopterin1h-pyrrole-3-propanoic acid, 5-[(1,2-dihydro-2-oxo-3h-indol-3-ylidene)methyl]-2,4-dimethyl-Ephedrine(3r,5s,7r,9s,10s,12s,13r,14s,17r)-17-((2r)-7-hydroxy-6-methylheptan-2-yl)-10,13-dimethylhexadecahydro-1h-cyclopenta[a]phenanthrene-3,7,12-triolRiluzoleThiamine pyrophosphateAmidosulfuronFlutriafolCyclopamine1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholineEprosartan1-stearoyl-2-linoleoyl-sn-glycerol2'-deoxyguanosine 5'-monophosphateAgmatine17-trifluoromethylphenyltrinorprostaglandin f2.alpha. isopropyl esterThifensulfuron-methylEpothilone bIndole-3-acetonitrileCytidine 5'-diphosphocholineThiamine monophosphateProstaglandin f2.alpha. dimethylamideAnabasineNg,ng-dimethyl-l-arginineLeukotriene f46-ketoprostaglandin f1.alpha.Hematoporphyrin1-stearoyl-rac-glycerolThunalbeneAsn-Leu-Arg(-)-atropineForskolin2'-deoxycytidineAlbiflorin2-linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamine1,2-dihexadecanoyl-sn-glycerolLoperamideTopiramatePentoxazoneGabapentinArtemisininFenfluramineCitrininGanoderic acid hPrilocaineS-adenosylmethionineEthanol, 2-[[3-(2,3-dichlorophenoxy)propyl]amino]-L-citrullineDaunorubicinN-tetracosenoyl-4-sphingenineTricyclo[3.3.1.1(3,7)]decane-1-carboxamide, n-[2-(benzoylamino)-6-benzothiazolyl]-TriethanolamineDfplnnh2-propylbenzimidazoleDimethoateOleoyl-l-carnitine1,1-dimethyl-4-phenylpiperazinium1-palmitoyl-2-linoleoyl-rac-glycerolNiacinamidePyridateCis-13-eicosenoic acid1-stearoyl-2-docosahexaenoyl-sn-glycerolNordihydroguaiaretic acidSecurinineEstra-1,3,5(10),7-tetraene-3,17.beta.-diolLatanoprostHc toxinPentaethylene glycolMevastatinSarafloxacinL-leucyl-l-prolineEllipticineCuminaldehyde4,4'-methylenebis(2,6-di-tert-butylphenol)20-hydroxyarachidonic acidMyristamine oxide2-hydroxyestradiolBerbamineN-3-hydroxyoctanoyl-l-homoserine lactone7,8-dehydropregnenoloneDiclosulam2-edahma [dmed-fahfa]N-acetylhistamineCreatineStreptomycin aPhytosphingosineDiaveridinePro-AlaCytosinePropranololBeauvericinPhosphamidon1-nitro-2-phenoxybenzeneMonomethyl tetrachloroterephthalate2-hydroxyatrazineO-phosphotyrosineLevamisoleArg-gluCurvularin2-naphthalenol, 1-[(2,4-dimethylphenyl)azo]-Bixafen1-palmitoyl-sn-glycero-3-phosphocholineSulfallateMetribuzin1-(4-methoxyphenyl)piperazineHypoxanthine1-hexadecanoyl-2-octadecadienoyl-sn-glycero-3-phosphocholineBisphenol a diglycidyl ether4-amino-2,6-dinitrotoluene1-palmitoyl-2-myristoyl-sn-glycero-3-phosphocholine1h-1,2,3-triazole-1-octanamide, n-[1,1'-biphenyl]-2-yl-4-(3-pyridinyl)-D-glutamineNevirapineAdenosine 5'-diphosphateEnterolactoneAndrostan-3-ol-17-one 3-glucuronidePimonidazolePristimerinN-stearoylsphinganineNorfenfluramineTomatidin1,2-dihexadecanoyl-sn-glycero-3-phosphocholinePhenylacetyl-l-glutamineHarmalineZerumbone1-pyrrolidineheptanoic acid, 2-(3-hydroxyoctyl)-5-oxo-2s-amino-4e-pentadecene-1,3r-diolGardenin bAdenineS-adenosyl-l-methionineDikegulac acidTubacinFructose2-arachidonoylglycerol1-Oleoyl-sn-glycero-3-phosphocholineOfuraceOmeprazoleIsocarbamid1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamineEnalaprilatHirsutineS-methyl-3-thioacetaminophenCocainePyridabenPro-Trp4-(methylnitrosamino)-1-(3-pyridyl)-1-butanolPrimaquinePipamperone4-cholestenoneCarnosolHexadecylacetylglycerolHistidineLys-Gly1h-indole-5-sulfonamide, n-(3-chlorophenyl)-3-[[3,5-dimethyl-4-[(4-methyl-1-piperazinyl)carbonyl]-1h-pyrrol-2-yl]methylene]-2,3-dihydro-n-methyl-2-oxo-, (3z)-NormeperidineStearamide.beta.-estradiol 17-valerateMetyraponeDiprotin bPyraflufen-ethyl1-hexadecyl-2-(9z-octadecenoyl)-sn-glycero-3-phosphocholine1-stearoyl-2-arachidonyl-sn-glycero-3-phosphocholineSufentanylIsobutyryl-l-carnitineAntipyrine1,3-benzenediol, 5-methyl-2-[(1r,6r)-3-methyl-6-(1-methylethenyl)-2-cyclohexen-1-yl]-MethyldopaFlavine mononucleotide1-methylpiperazineTimosaponin a-iiiGlycerophosphocholine3-methyladenineCapecitabineQuinoclamineNorharmane1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine2-docosahexaenoyl-1-palmitoyl-sn-glycero-3-phosphoethanolaminePropazine1-myristoyl-sn-glycero-3-phosphocholine1-palmitoyl-2-glutaryl phosphatidylcholineS-adenosyl-l-homocysteine16-phenyltetranorprostaglandin e1Ancymidol1-palmitoyl-2-thiopalmitoyl phosphatidylcholine(r)-(+)-arachidonyl-1'-hydroxy-2'-propylamidePyridoxamine2,4,6-tri-tert-butylaniline4-(4-aminophenoxy)anilineDidodecyl 3,3'-thiodipropionateBergamotin2-((4r)-4-((5r,7r,8r,9s,10s,12s,13r,17r)-7,12-dihydroxy-10,13-dimethyl-3-oxohexadecahydro-1h-cyclopenta[a]phenanthren-17-yl)pentanamido)ethane-1-sulfonic acidN-arachidonylmaleimide1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine2-hydroxy-5-nitrobenzyl alcoholLinoelaidic acidN-nitrosodimethylamineCordycepinL-palmitoylcarnitineProtriptylineSalinomycinIsopropalinAzatadineCholineAsparagineAcetylcholineAdenosine 5'-monophosphateFluoreneMethaqualoneDibutyl phthalate.beta.-homoproline1h-1,2,4-triazol-3-amineHomatropineIndirubin-3'-monoximeN-(1-amino-3,3-dimethyl-1-oxobutan-2-yl)-1-pentyl-1h-indole-3-carboxamideEthyl 3-indoleacetateOxcarbazepineTerbutalineTramadol1-amino-2-ethoxybenzeneSulfentrazone1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholineChrysosplenetinMethylecgonine1-[5-(trifluoromethyl)-2-pyridinyl]-4-piperidinamine1-o-hexadecyl-2-deoxy-2-thio-s-acetyl-sn-glyceryl-3-phosphorylcholineMenadioneHaloxyfopDaurisolineAcacetin6',7'-dihydroxybergamottinErucamide4-hydroxyhexenalPalmitoylcarnitineN,n-dimethylformamideTiclopidine1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholineHistamineMiltefosineN-nervonoyl-d-erythro-sphingosylphosphorylcholineSolasodine4-benzofuranethanamine, 2,3-dihydro-.alpha.-methyl-OxypurinolProtoporphyrin ixHeptadecasphinganinePro-pro4'-o-.beta.-d-glucosyl-5-o-methylvisamminol17.beta.-nandrolone decanoateFenamiphosHetisineN,n,n-trimethyllysineGly-AsnLauryldimethylamine oxideLeucine3-methyl-l-histidineD-glucose 6-phosphateEvodiamineN-(3-oxooctanoyl)-l-homoserine lactone5-methyl-7-methoxyisoflavone7-demethylsuberosinArg-Pro-ArgSenecionine1-lignoceroyl-2-hydroxy-sn-glycero-3-phosphocholine1-methyl-l-histidineDesferrioxamine d2Fortunellin1-heptadecanoyl-sn-glycero-3-phosphocholine1-docosahexaenoyl-2-stearoyl-sn-glycero-3-phosphocholine1-acetylimidazoleFluazifopPiperidinePyrrolidineM-cresolAlbizziinMonoelaidinDL-serinePrometryneThiamet gN-lauroyl-d-erythro-sphingosylphosphorylcholineTrp-Arg-ArgArecaidine1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phospho-(1'-rac-glycerol)Terfenadine10-nitrooleic acidPhosphocreatine1-methyl-2-undecylquinolin-4-oneImazethapyrPhosphocholineN-acetylhistidinePaclobutrazolAlbendazoleAnisomycinIsatinN-acetyl-l-phenylalanine3-hydroxyxanthone1,2-dilauroyl-sn-glycero-3-phosphatidylcholineAlitamefalseUntargeted Metabolomic Profiling of Left Ventricular Myocardium in a Mouse Model of Diabetic CardiomyopathyThis study characterizes metabolic alterations in the left ventricular myocardium of a high-fat diet and streptozotocin-induced mouse model of diabetic cardiomyopathy. Untargeted LC–MS/MS metabolomics was performed using a Vanquish UHPLC system coupled to an Orbitrap Exploris 120 mass spectrometer. Data were processed using XCMS and analyzed by principal component analysis, OPLS-DA, differential metabolite screening, hierarchical clustering, and KEGG- and MetaboAnalyst-based pathway analysis. Pooled quality-control samples were used throughout the analytical sequence to monitor system stability.2026-07-202026-07-15MTBLS15043