MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/m_MTBLS15049_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/m_MTBLS15049_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/a_MTBLS15049_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/a_MTBLS15049_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/s_MTBLS15049.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072705-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072704-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072703-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072709-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072719-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072715-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072712-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072711-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072710-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072714-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072720-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072708-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072707-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP1_FZTM240072706-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN2_FZTM240072713-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072707-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072705-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072703-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072706-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072704-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072709-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072718-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072716-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072718-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072717-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072717-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072714-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072720-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072716-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN3_FZTM240072715-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072711-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP3_FZTM240072719-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072710-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072713-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CP2_FZTM240072712-1A.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049/FILES/RAW_FILES/HFX8_CN1_FZTM240072708-1A.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS15049<p>The raw data files generated by UHPLC-MS/MS were processed using the Compound Discoverer 3.3 (CD3.3, ThermoFisher) to perform peak alignment, peak picking, and quantitation for each metabolite. The main parameters were set as follows: peak area was corrected with the first QC, actual mass tolerance, 5 ppm; signal intensity tolerance, 30 percent; and minimum intensity, et al. After that, peak intensities were normalized to the total spectral intensity. The normalized data was used to predict the molecular formula based on additive ions, molecular ion peaks and fragment ions. And then peaks were matched with the mzCloud (https://www.mzcloud.org/), mzVault and MassList database to obtain the accurate qualitative and relative quantitative results. Statistical analyses were performed using the statistical software R (R version R-3.4.3), Python (Python 2.7.6 version) and CentOS (CentOS release 6.6). When data were not normally distributed, standardize according to the formula: sample raw quantitation value / (The sum of sample metabolite quantitation value / The sum of QC1 sample metabolite quantitation value) to obtain relative peak areas; and compounds whose CVs of relative peak areas in QC samples were greater than 30 percent were removed, and finally the metabolites' identification and relative quantification results were obtained.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (ThermoFisher, Germany) in Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypersil Goldcolumn (100 by 2.1 mm, 1.9 mum) using a 12-min linear gradient at a flow rate of 0.2 mL/min. The eluents for the positive and negative polarity modes were eluent A (0.1 percent FA in Water) and eluent B (Methanol). The solvent gradient was set as follows: 2 percent B, 1.5 min; 2 to 85 percent B, 3 min; 85 to 100 percent B, 10 min; 100 to 2 percent B, 10.1 min; 2 percent B, 12 min. </p>Multi-omics analysis reveals the modulatory effects of Moringa oleifera ethanol extract on gut microbiota and metabolic profiles in broilers.Yunnan Institute of Tropical CropsFuhong LEIfecesmass spectrometry assay<p>Tissue sample</p><p><br></p><p>Tissues (100 mg) were individually grounded with liquid nitrogen and the homogenate was resuspended with prechilled 80 percent methanol by well vortex. The samples were incubated on ice for 5 min and then were centrifuged at 15,000 g, 4 degrees Celsius for 20 min. Some of supernatant was diluted to final concentration containing 53 percent methanol by LC-MS grade water. The samples were subsequently transferred to a fresh Eppendorf tube and then were centrifuged at 15,000 g, 4 degrees Celsius for 20 min. Finally, the supernatant was injected into the LC-MS/MS system analysis.</p>Gallus gallushttps://www.ebi.ac.uk/metabolights/MTBLS15049Fuhong Lei. Yunnan Institute of Tropical Crops. 1759669628@qq.com.Zubing Zhang. Yunnan Institute of Tropical Crops. sbnzzb@126.com.<p>The raw data files generated by UHPLC-MS/MS were processed using the Compound Discoverer 3.3 (CD3.3, ThermoFisher) to perform peak alignment, peak picking, and quantitation for each metabolite. The main parameters were set as follows: peak area was corrected with the first QC, actual mass tolerance, 5 ppm; signal intensity tolerance, 30 percent; and minimum intensity, et al. After that, peak intensities were normalized to the total spectral intensity. The normalized data was used to predict the molecular formula based on additive ions, molecular ion peaks and fragment ions. And then peaks were matched with the mzCloud (https://www.mzcloud.org/), mzVault and MassList database to obtain the accurate qualitative and relative quantitative results. Statistical analyses were performed using the statistical software R (R version R-3.4.3), Python (Python 2.7.6 version) and CentOS (CentOS release 6.6). When data were not normally distributed, standardize according to the formula: sample raw quantitation value / (The sum of sample metabolite quantitation value / The sum of QC1 sample metabolite quantitation value) to obtain relative peak areas; and compounds whose CVs of relative peak areas in QC samples were greater than 30 percent were removed, and finally the metabolites' identification and relative quantification results were obtained.</p>Group1759669628@qq.com<p>By feeding MOE and BZ, the feces in the cecum of the broilers were collected for analysis. Cecal contents were extracted with 80 percent methanol and analyzed by LC-MS/MS (Positive and negative ion mode, m/z 70 to 1050) on an ACQUITY UPLC HSS T3 column with a 14-minute gradient. Metabolite annotation was performed using KEGG and LIPID MAPS databases.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsmicrobial metabolomicsuntargeted analysiscecal contentbroilersQExactiveHF/QExactiveHF-Xbacitracin zincgut microbiotacecalMoringa oleifera ethanolic extractGallus gallusThermo Scientific Vanquish UHPLC SystemMetabolomicsmicrobial metabolomicsuntargeted analysiscecal contentbroilersQExactiveHF/QExactiveHF-Xbacitracin zincgut microbiotacecalMoringa oleifera ethanolic extractGallus gallus<p>Analyses were performed using an Orbitrap Q ExactiveTM HF mass spectrometer or Orbitrap Q ExactiveTMHF-X mass spectrometer (Thermo Fisher, Germany) in Novogene Co., Ltd. (Beijing, China). Q Exactive HF mass spectrometer was operated in positive/negative polarity mode with spray voltage of 3.5 kV, capillary temperature of 320 degrees Celsius, sheath gas flow rate of 35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heater temperature of 350 degrees Celsius.</p>Corticosterone2,3-Dinor-8-epi-prostaglandin F2¦ÁProstaglandin G2PhenylacetylglutamineLNAPE 17:0/N-2:0LPA 14:0Dl-3,4-Dihydroxymandelic AcidCytidine-5'-monophosphateLysopa 16:0PC 11:0_17:0¦Â-Estradiol-17¦Â-glucuronideDeoxycholic AcidPE O-24:6_16:0LPE O-14:1Cer 15:2;3O/19:0;(2OH)Prostaglandin H1Prostaglandin H22,3-Dinor-TXB23-anilino-5-(4-chlorophenyl)cyclohex-2-en-1-oneCitrulline6-chloro-2-methyl-4-[(2,3,4,5,6-pentamethylbenzyl)oxy]quinoline(¡À)8(9)-DiHETCapric acidPA 24:0_20:4D-Glucuronic acidLPE 13:08-iso-15-keto Prostaglandin E2(+/-)-CP 47,497-C7-Hydroxy metaboliteCapryloylglycine(¡À)18-HEPENeopterinMGDG O-16:1_2:0N-Acetyl-D-tryptophan(¡À)11-HETELPS 17:1MGDG 4:0_15:0Prostaglandin E3Estradiol20-Carboxy-Leukotriene B42-Amino-3-(4-hydroxy-3-methoxyphenyl)propanoic acidMevalonic acidN1-(6-methyl-4-oxo-3,4-dihydroquinazolin-2-yl)-4-nitrobenzamideN1-[3-(trifluoromethyl)phenyl]-4-chlorobenzamide5'-Adenylic acidN-Carbamyl-L-glutamicacidN-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy)phenyl]ureaSL 17:0;O/14:0all-cis-4,7,10,13,16-Docosapentaenoic acidTetrahydrocorticosteroneD-(+)-Maltose3-(4-benzyl-5-thioxo-4,5-dihydro-1H-1,2,4-triazol-3-yl)piperidin-2-oneCer 14:0;2O/20:1;(3OH)(FA 18:1)PG O-15:1_14:0tetranor-PGFMN-(4-chlorophenyl)-N'-cyclohexylthioureaDeoxyribose 5-PhosphateN-Acetylneuraminic acidPantothenic aciddiethyl 2-(1-methyl-3-nitro-2-oxo-1,2-dihydroquinolin-4-yl)malonateIndoxylsulfuric acid13,14-Dihydro-15-keto prostaglandin F2¦Á3-[4-(tert-butyl)anilino]-2-(3-thienylcarbonyl)acrylonitrile5-[4-(tert-butyl)phenoxy]-6-nitroisoindoline-1,3-dioneN1-(2-methyl-3-nitrophenyl)cyclohexane-1-carboxamideXanthurenic acid12(S)-HETED-Glucosamine 6-phosphateLPA 16:03-(1,1,2,3,3,3-hexafluoropropyl)adamantane-1-carboxylic acidSpiculisporic AcidPC 11:0_15:0PC 14:0_15:019-NortestosteroneS-LactoyglutathioneLPE 22:4N2-(3-methylphenyl)-5,5-dimethyl-4,5-dihydro-1,3-thiazol-2-amineEthyl chrysanthemumate11(Z),14(Z)-Eicosadienoic AcidMGDG O-22:4_20:5HexCer 23:0;2O/9:0Prostaglandin D3Asp-glu8Z,11Z,14Z-Eicosatrienoic acidtrans-Petroselinic Acid2-PhenylglycineOrnithineAsaraldehydeSulfaquinoxaline3-Hydroxybenzoic acidLPS 15:0LPS 15:1N1-phenethylbenzene-1-carbothioamide(¡À)10(11)-EpDPAProstaglandin A310-NitrolinoleateDL-3,4-Dihydroxyphenyl glycolPC 12:0_18:0Acetyl-N-formyl-5-methoxykynurenaminebeta-Nicotinamide mononucleotideEstrone sulfateMethyl acetoacetateCer 15:2;3O/22:1;(2OH)2-(1H-indol-3-yl)-3-[4-(trifluoromethyl)phenyl]acrylonitrileST 29:2;O;S3-Hydroxy-3-methylglutaric acid23-Norcholic acidPG O-16:1_15:0LPG O-20:4PEtOH 19:0_20:5LPG O-20:3D-Erythrose 4-phosphatePE 18:0_20:4LPG O-20:21,5-Anhydro-D-glucitol3-Hydroxybutyric acidMGDG O-24:6_20:5PEtOH 22:0_20:42-IsopropylmalateHydrocortisone acetateBenzoic acid4-Hydroxyretinoic AcidAscorbic acid2-Hydroxy-2-methyl-3-buten-1-yl beta-D-glucopyranosideMethylsuccinic acidTetradecanedioic acidIsoproterenolN-Acetyl-D-galactosamineMenaquinoneOleanolic acidLPE 20:1LPG O-18:3O-AcetylserineLPE 20:2LPE O-18:0LPE 20:3LPE 20:4LPE O-18:1LPE O-18:2LPE O-18:3LPG O-18:5LPE 20:5N-Acetyl-L-tyrosineNAGly 15:0/15:0¦Ä-Ribono-1,4-lactoneLPI 16:0S-Sulfo-L-cysteinegamma-GlutamylmethionineGibberellin A7Gibberellin A44-(octyloxy)benzoic acidMethyl-beta-galactopyranosideMethyl EudesMateCer 15:0;2O/16:0;(2OH)ST 28:2;O;S15-Deoxy-¦Ä12,14 -Prostaglandin J2trans-Cinnamic acid11-dehydro Thromboxane B2PhenylacetaldehydeLPS 13:0MGDG O-24:5_20:5L-Threonic acid2,3,4-Trihydroxybenzoic acidDGDG O-26:6_2:0ButylparabenRifampicinHexCer 22:3;2O/17:1;OAdenosine 5'-DiphosphatePG 4:0_16:0N-Acetylphenylalanine4-Methoxycinnamic Acid11(E)-Eicosenoic AcidXanthosineD-Threose3-amino-2,6-diphenyl-4,7-dihydro-2H-pyrazolo[3,4-d]pyrimidin-4-oneCitric acidLPG 20:5PG O-20:1_16:1LPG 20:32-ThiocytidineLPG 20:4LPG 20:1PG O-15:1_18:1LPG 20:2PG O-14:1_15:0ST 28:1;O;Hex;FA 20:4LPG 20:0FAHFA 20:2/22:42-(acetylamino)-4-(methylthio)butanoic acidTauroursodeoxycholic acidLPE O-16:1LPE O-16:2NAGlySer 15:0/16:02'-DeoxyuridineStearic acidCer 14:0;2O/19:1;(3OH)(FA 18:2)ST 28:2;O;Hex;FA 20:55-AminopentanoateOrotidineChenodeoxycholic acid-3-beta-D-glucuronideTretinoinST 24:1;O4;T/18:1LPI 14:02-Hydroxycaproic acidFAHFA 15:0/20:4FAHFA 15:0/20:311-KetoetiocholanoloneAdrenosteroneCer 17:0;2O/17:0;(3OH)1-Palmitoyl-Sn-Glycero-3-PhosphocholineAsp-Phe2'-Deoxyadenosine 5'-monophosphate (dAMP)Monobutyl phthalateOfloxacin impurity ETaurochenodeoxycholic acidFAHFA 22:4/18:0Cer 18:1;2O/15:0J14717alpha-Ethinyl estradiolL-Adrenaline4-hydroxy-1-phenyl-1,2-dihydroquinolin-2-oneLactobionic acidFAHFA 17:1/20:4cis-Aconitic acidTaurolithocholic acid 3-sulfateLPG 11:03-[4-methyl-1-(2-methylpropanoyl)-3-oxocyclohexyl]butanoic acidProstaglandin J2LPS 22:6Cer 18:1;3O/15:0(3R)-4,4-Dimethyl-2-oxotetrahydro-3-furanyl beta-D-glucopyranoside1-Methyluric acidCetirizine N-oxide1-(2,4-difluorobenzoyl)-4-piperidinecarboxylic acidN-Acetylglucosamine 1-phosphateD-RiboseTridecylic acidLPG 22:54-Hydroxyphenylacetic acidEthyl myristateLPG 22:3aminoimidazole carboxamide ribonucleotideLPG 22:4(¡À)9(10)-DiHOMECer 18:0;2O/17:0;(3OH)2-AminobenzenesulfonamideTaurineDocosanoic Acid12-epi Leukotriene B4Hydrocinnamic acidArachidic acidDL-4-Hydroxyphenyllactic acid17¦Á-HydroxypregnenoloneN1-[4-(cyanomethyl)phenyl]-4-chlorobenzamideHBMP 18:2_20:5_12:0N-AcetylsphingosineFAHFA 18:2/17:0Cer 17:0;2O/16:0;(3OH)(¡À)9(10)-EpOMEN'-(cyclohexylcarbonyl)-4-methyl-1,2,3-thiadiazole-5-carbohydrazideFAHFA 18:2/17:2LPG 12:0D-Alanyl-D-alanine2-[(3S)-1-(Cyclohexylmethyl)-3-pyrrolidinyl]-5-fluoro-1H-benzimidazolealpha-Ketoglutaric acidLPG O-14:1LPG O-14:0LPC 14:0ST 24:2;O4Chlorogenic Acid Methyl Ester15-Epiprostaglandin E1Docosatrienoic acidN-Acetyl-Asp-Glu8-HydroxyguanosineFAHFA 20:3/20:3N1-(3-pyridyl)-2,3,4,5,6-pentamethylbenzene-1-sulfonamideCer 17:1;2O/18:1;(3OH)Catechol5(S),15(S)-DiHETE4-OxoprolineN6-Succinyl Adenosine2-Oxoglutaric acid1-MethylguanosineLysopc 16:1ADBICA N-pentanoic acid metaboliteUndecanoic acidAICA ribonucleotide15-keto Prostaglandin E11-acetyl-N-(6-chloro-1,3-benzothiazol-2-yl)-4-piperidinecarboxamide2'-Deoxycytidine 5'-monophosphate (dCMP)Dibenzoyl ThiamineCystine3-Hydroxyvaleric acidMGDG O-28:6_18:1MGDG O-20:1_20:5PG 3:0_16:0Indole-2-carboxylic acidBenzyl 6-O-beta-D-glucopyranosyl-beta-D-glucopyranosideMethyl-2-hydroxyisobutyric acidFAHFA 20:5/16:0FAHFA 20:5/16:2Thymidine 5'-monophosphateThymidine 3,5-cyclic monophosphatePG O-18:2_16:0PG 16:0_21:22-MethoxyestradiolPC 16:1_20:5Dibutyl sebacateEicosapentaenoic acidMGDG O-26:6_20:5PG O-17:1_16:08,12-iso-iPF2¦Á-VI2-chloro-6-[(2-oxoazepan-3-yl)amino]benzonitrileMycophenolic acid5,6-dimethyl-3-[5-(trifluoromethyl)pyridin-2-yl]-1,2,4-triazineD-Xylonic AcidL-KynurenineFAHFA 22:6/17:2LPG O-16:3IDPPG O-19:1_20:5LPG O-16:0MGDG O-24:1_20:4PEtOH 22:1_20:54-(trifluoromethyl)nicotinic acidST 28:1;O;Sdelta9-THC-d3L-Cystine2-({[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]imino}methyl)phenolGlucosamine 6-sulfateN-Acetyl-5-aminosalicylic acidLauric acid ethyl esterN-acetyl-D-glucosamine(2-anilino-4-methyl-1,3-thiazol-5-yl)(4-methoxyphenyl)methanone17-AAGLPE 19:1L-MalateAdipic acidGluconolactoneIsorhapontigeninAdrenic acidMethylgallateLysopc 14:0MGDG O-8:0_20:4MGDG O-8:0_20:5MGDG 2:0_15:0Homovanillic acidST 28:1;O;Hex;FA 18:2Nicotinuric AcidProstaglandin F2¦ÂQuinolinic acidMGDG O-24:6_16:1FAHFA 18:1/20:3N1-(4-chlorophenyl)-2-cyano-4,4-dimethyl-3-oxopentanamideLPI 17:0N'1-[1-(2-hydroxyphenyl)ethylidene]-3-methoxybenzene-1-carbohydrazide4-fluoro-N-[4-(4-methylpiperazino)phenyl]benzenesulfonamideFAHFA 20:5/18:3(¡À)11(12)-DiHET4,5-diphenyl-2,3-dihydro-1H-pyrazolo[3,4-c]pyridazin-3-oneCitraconic acidFAHFA 20:4/17:2PC 9:0_9:0Cyclic ADP-riboseHexCer 24:1;2O/9:0N-Acetyl-D-galactosamine 4-sulfateCorchorifatty acid FOxoadipic AcidN-Acetyl-L-phenylalanine4-methoxy-N-[2-(4-methoxyphenoxy)-5-(trifluoromethyl)phenyl]benzamideFAHFA 2:0/17:02,3-dinor Prostaglandin E116-Hydroxyhexadecanoic acidalpha-D-Glucopyranosyl 2-O-(2-methylbutanoyl)-alpha-D-glucopyranosideLPG 16:1LPG 16:0LPG 16:2Sodium cholate4,5-Dicaffeoylquinic acidEsculin(¡À)8-HEPENervonic acidMGMG (18:2)12-Hydroxydodecanoic acidAdenosine diphosphate ribose(3-Methoxy-4-hydroxyphenyl)ethylene glycol sulfate3-Methoxyphenylacetic acidAsp-Phe methyl esterN4-AcetylcytidineLPE 17:2FormononetinPG 15:0_18:13-Methylglutaric acidRoyal jelly acidHexanoic acidDesoxycortonePG O-19:2_15:0Glycerol-3-phosphate2-{2-oxo-2-[(2-oxo-3-azepanyl)amino]ethoxy}acetic acidPG O-20:1_20:5Resolvin E14-MethylphenolSalicylic acid15(R)-Prostaglandin E2N-AcetylhistidinePE 18:0_22:5;O5-Aminovaleric acidPyrithioxinD-¦Á-Hydroxyglutaric acidMGDG O-26:3_20:4D-GlucosamineCer 15:0;2O/15:0ST 24:1;O4;Tbeta-Estradiol 17-AcetateMGDG O-18:4_3:0L-Methionine sulfonePerfluorooctanoic acid (PFOA)2-(1,3-dimethyl-1H-pyrazol-5-yl)-1H-isoindole-1,3(2H)-dione1-(4-fluorophenyl)-2-(4-methoxyphenyl)-4-(2-naphthyl)butane-1,4-dioneTHJ2201 N-pentanoic acid metaboliteSDMAAcetylcysteineAldosterone¦Ä-Gluconic acid ¦Ä-lactone5-Phenyl-N-(4-(trifluoromethyl)phenyl)oxazol-2-amineLPG 18:2L-Glutathione (reduced)LPG 18:0NAGlySer 18:3/15:1Maleic acidCer 18:1;2O/5:0Flavin adenine dinucleotide (FAD)Inosine 5'-MonophosphateSL 17:0;O/16:1;O3,4-dihydro-2H,6H-[1,3]thiazino[2,3-b]quinazolin-6-oneGluconic acidN-AcetylglycineN-Acetyl-D-alloisoleucineUndecanedioic acidMGDG 15:0_15:1Phenobarbital3-[3-(beta-D-Glucopyranosyloxy)-2-methoxyphenyl]propanoic acidCer 16:0;2O/16:0;(3OH)N-¦Á-Acetyl-L-asparagineSalvinorin BSalvinorin ADl-Lanthionine¦Â-Muricholic acidL-Aspartic acidLysopc 18:3MGDG O-17:2_3:0LPE 15:0Lysopc 18:1LPE 15:11a,1b-Dihomo prostaglandin E1albiflorinCitramalic acid3-Oxo-7alpha,12alpha-hydroxy-5beta-cholanoic acidD-Glucono-1,5-lactone4-Hydroxyphenylpyruvic acidTrehalose 6-phosphategamma-Glu-TyrRibose2-(Dimethylamino)GuanosineCannabichromevarinLPC O-17:1H-Gly-Pro-OHLPC O-17:02-Hydroxymyristic acidDimetghyl 4-Hydroxyisophthalate5-Methoxyindole-3-CarbaldehydeVitamin U3-Guanidinopropionic acidLPA 22:62,3-Dihydroxybenzoic acid11-keto Testosterone (CRM)IrinotecanN-Isobutyrylglycinelithocholic acid 3-sulfate(¡À)9-HpODEXMPMestranol2'-Deoxyguanosine 5'-monophosphate (dGMP)(+/-)-Equolalpha-Farnesene3-Isopropylmalic acidLeu-ProLPS 16:12'-O-MethyluridineN1-(3,5-dichlorophenyl)-3-morpholinopropanamide(2R)-2,3-Dihydroxypropanoic acidN1-[4-(acetylamino)phenyl]-2,2-dimethylcyclopropane-1-carboxamideFolic acidLysope 14:0DeoxyinosinePC O-18:3_18:2DL-Mandelic acid13,14-dihydro Prostaglandin F1¦ÁGamma-Caprolactone2-Butoxyacetic acidN-Acetylvaline5¦Á-DihydrotestosteroneHexanoylglycineDl-Glyceraldehyde3-phosphateD-(-)-Quinic acid3,8,9-trihydroxy-10-propyl-3,4,5,8,9,10-hexahydro-2H-oxecin-2-onePhenylglyoxylic acidHydroxyflutamideBiotin4-Anisic acidN2-MethylguanosineD-RaffinoseFAHFA 18:2/20:4PG O-15:1_15:0Syringic acidN-Acetylaspartic acidMesaconic acidN1-(3-amino-4-chlorophenyl)-2-[2,4-di(tert-pentyl)phenoxy]acetamide5-(2-chloro-6-methylphenoxy)-1,3-dimethyl-4-nitro-1H-pyrazole3-(4-chlorophenyl)-5-(3-phenyl-1H-pyrazol-4-yl)-1,2,4-oxadiazole3-Hydroxyanthranilic acidHexCer 24:0;2O/9:09-HOTrE2-deoxyglucose-6-phosphatetrans-10-Heptadecenoic AcidFAHFA 20:5/20:3N-(2,6-diethylphenyl)-N'-(2-methoxyphenyl)thioureaTHCFAHFA 20:5/20:4Guanosine monophosphateGlutaric acid4-EthylphenolEstriol2'-O-MethyladenosineCer 20:1;2O/16:0FAHFA 20:4/22:4Aflatoxin G1LPE O-13:1FAHFA 20:4/22:5LPI 11:05-Hydroxytryptophol4-((5-(4-Nitrophenyl)oxazol-2-yl)amino)benzonitrile1-methyl-N-(4-piperidinophenyl)-1H-imidazole-4-sulfonamideEpoxomicin2-methyl-4-{2-[3-(trifluoromethyl)phenyl]diaz-1-enyl}anilineTris(2-carboxyethyl)phosphine2-[(carboxymethyl)(methyl)amino]-5-methoxybenzoic acidLPE 14:04-Pyridoxic acidGuanosineN-Acetyl-L-methionine2-(1H-benzimidazol-2-yl)-3-phenylacrylonitrileKynurenic acid O-hexside5-[4-(4-methoxyphenoxy)phenyl]-1H-pyrazoleCer 19:0;2O/12:0;O(FA 18:2)N1-(4-chlorophenyl)-3-(1H-pyrrol-1-ylmethyl)piperidine-1-carboxamideChenodeoxycholic AcidPG 4:0_15:0Hexadecanedioic acid17¦Á-HydroxyprogesteroneUridineN-benzyl-N'-methyl-N-(pyridin-3-ylmethyl)thioureaFmoc-L-IsoleucineLPC 20:5FAHFA 20:5/22:5Prostaglandin E1 EthanolamideMalonic acidDecanoic acidEquol7-(1-pyrrolidinyl)pyrimido[4,5-d]pyrimidin-4-amineDulcitolcGMPLPI 20:3LPI 20:4LPI 20:5LPC 20:2LPA 15:0FAHFA 20:4/20:2H-Tyr(3-I)-OHST 27:1;O;Hex;FA 20:5FAHFA 20:4/20:4geranyl ppFAHFA 20:4/20:31-Caffeoylquinic AcidEthyl-¦Â-D-glucuronideN'-(benzoyloxy)-2-(2,2-dichlorocyclopropyl)ethanimidamide15-Deoxy-¦¤12,14-prostaglandin D2Aspartic acid1-benzyl-3-(2-methylphenyl)-3,7-dihydro-1H-purine-2,6-dioneGlycoursodeoxycholic acidCytidine 5'-Monophosphate-N-Acetylneuraminic Acid3-methyl-5-oxo-5-(4-toluidino)pentanoic acidLysope 18:1FAHFA 16:1/18:3Fumaric acidPG O-18:1_13:0Cyclohexaneacetic acidPI-Cer 12:0;2O/22:0;OPhenylpyruvic acidFAHFA 4:0/17:0Phe-Pro2-Methylbutyl beta-D-glucopyranoside15(R),19(R)-hydroxy Prostaglandin E1MGDG O-28:7_20:5PhloretinOleoyl-L-¦Á-lysophosphatidic acid(+/-)13-HODES-(Methyl)Glutathione4-amino-6-chloro-3-cinnolinecarboxamideTaurocholic acidFolinic acidFAHFA 2:0/20:02-Isopropylmalic acid1-methyl-N-(3-methyl-5-cinnolinyl)-1H-imidazole-4-sulfonamide5 methyl THF5-Dehydroquinic acid¦Á-EstradiolFAHFA 18:3/16:2PG O-21:2_20:4Suberic acidJNJ-1661010MGDG O-16:4_12:0cis-2-Decenoic acidLPA 17:0Cer 18:0;2O/16:0;(3OH)4-AminoindoleLPG O-19:2Epinephrine bitartrateLPE O-17:1LPE O-17:2N-Acetyl-L-glutamine(¡À)11(12)-EETN'-[4-(trifluoromethyl)benzoyl]-6-quinoxalinecarbohydrazideN-Acetyl-aspartic acidDocosahexaenoic AcidCreatine phosphateLPI 15:0N-(1,3-benzodioxol-5-yl)-7-chloroquinolin-4-aminePimelic acidcis-7-Hexadecenoic acid5-[(Benzoyloxy)methyl]-4,5,6-trihydroxy-2-cyclohexen-1-yl benzoateGlycolithocholic acidGibberellic acid13Z,16Z-Docosadienoic AcidCUMYL-PICA N-pentanoic acid metabolitetetranor-12(R)-HETEPC O-20:5_3:0Homocysteic acidBilirubinMGDG O-22:2_20:5Cer 17:2;2O/18:1;(3OH)cholesteryl sulfate4-phenylbenzo[4,5]imidazo[1,2-a]pyrimidin-2-amine(+/-)11-HETEPG 16:0_22:6;OL-AscorbateHesperetinN-Acetyl-DL-glutamic acid3-Phenyllactic acidCer 18:1;2O/16:0;(3OH)Benzyl cinnamatePC O-16:0_20:5PC O-16:0_20:4Acetaminophen glucuronideIndole-3-lactic acidPG O-20:1_15:02-Keto-4-methylthiobutyric acidP-Aminohippuric Acid2-Aminoadipic acidMGDG O-28:1_18:2Monobenzyl phthalateHydrocortisoneLPE O-15:11a,1b-Dihomo prostaglandin F2¦Á4-Methylvaleric AcidMGDG O-26:4_20:4N alpha-Acetyl-L-ArgininePC 16:0_18:2PG O-24:6_20:5LPI 13:07-Ketolithocholic acidFAHFA 18:0/2:02-Hydroxy-1-(4-methoxyphenyl)propyl hexopyranosideN-AcetylalanineCer 18:1;2O/14:0NaringeninAllantoinXanthohumolLPG 9:0MGDG 2:0_14:0MAG (18:2)Tetrahydroaldosterone5-HydroxytryptophanL-cysteineProstaglandin F3¦ÁLPC O-14:0DL-Malic acidMGDG O-24:5_3:0Methyl 3-indolyacetatePicrotin2'-Deoxyinosine5-Methoxyindoleacetic acidMuramic acidN-lactoyl-phenylalaninePorphobilinogenPG O-18:2_15:0TyrosolN2,N6-di(4-methoxyphenyl)-3-nitropyridine-2,6-diamineL-ArabinitolGuggulsterone2'-DeoxycytidineOxazepam-d5Lysops 22:5(¡À)12(13)-DiHOMELauric acidCitrinin16¦Á-HydroxyestroneDocosapentaenoic acidLPG 13:0PE 13:0_15:0LPG 13:1Lithocholic Acid3-Methyladipic acidPentadecanoic acidLPG O-15:1LPG O-15:0LPS 20:3Glu-GlnLPS 20:45-(3-chloro-4-methylanilino)-1-methyl-1H-pyrazol-3-olFAHFA 18:0/20:2alpha-Benzylsuccinic acidPhenylacetylglycineHBMP 18:2_18:2_12:0DL-m-Tyrosine2-(Formylamino)Benzoic AcidMGDG O-21:2_20:5(¡À)19(20)-DiHDPA4-ThiouridineCer 17:0;2O/16:0;(2OH)Kinetin 9-ribosideLysoPE 18:0N-AcetylmannosamineGlu-GluLysopc 17:0¦Á,¦Á-TrehaloseCannabidiolic acidMGDG 2:0_16:01,2,3-Benzenetricarboxylic acid8-iso-15-keto Prostaglandin F2¦Á2-Hydroxyphenylalanine17(S)-HpDHA4-hydroxy-1-methyl-3-(phenylthio)-1,2-dihydroquinolin-2-one¦¤17-6-keto prostaglandin F1¦ÁPC 16:0_15:16,15-diketo-13,14-dihydro Prostaglandin F1¦ÁProstaglandin F1¦ÂFAHFA 20:5/17:2LPI 18:2LPI 18:1DehydroepiandrosteroneNicotinate ribonucleoside1,2-Dipalmitoylphosphatidylglycerol5-Sulfosalicylic acidSuccinic acidProstaglandin A1 ethyl esterFAHFA 20:4/18:2Cer 15:2;3O/22:0;(2OH)(+/-)8(9)-DiHET4-[2-(2-oxo-1-imidazolidinyl)ethyl]-1lambda~6~,4-thiazinane-1,1-dione15-OxoEDEMGDG O-22:4_15:05-trans-Prostaglandin E2N-[5-(trifluoromethyl)-2-pyridinyl]benzenesulfonamides7p2-(2-carboxy-2-methylpropyl)-4,6-dimethylbenzoic acidFAHFA 17:0/19:2(2S)-4-Oxo-2-phenyl-3,4-dihydro-2H-chromen-7-yl beta-D-glucopyranoside15(R)-Lipoxin A4LPG O-17:2LPG 15:1FAHFA 18:2/18:27,8-DihydrofolateLPG 15:05-(3,4-dihydroxybenzylidene)hexahydropyrimidine-2,4,6-trione5-MethyluridineSL 18:0;O/16:1;O5-Hydroxyindole-2-carboxylic acidCer 17:0;2O/14:0Cer 18:1;2O/17:0;(3OH)N1-[3-(1H-imidazol-1-yl)propyl]-2-[(4-nitrobenzoyl)amino]benzamideVerbascose11(Z)-Eicosenoic acid8(S)-Hydroxy-(5Z,9E,11Z,14Z)-eicosatetraenoic acidFAHFA 20:5/3:0Uridine monophosphate (UMP)N-[4-(diethylamino)phenyl]-N'-phenylurea16-(Hexopyranosyloxy)-7-hydroxy-8,9-epoxypimaran-18-oic acidMethioninesulfoxideCer 16:1;2O/16:0;(3OH)Glycerophospho-N-palmitoyl ethanolamineOuabaindiethyl 2-[(2-morpholinoanilino)methylidene]malonateCholic acidArachidonic acid2'-Deoxyadenosine 5'-monophosphate19(R)-Hydroxy-prostaglandin E2Adenosine 5'-diphosphoglucose3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acidN-Oleoyl GlycineEthylmalonic acidCaffeine13-Hpotre(R)N-(1,2,3,4-tetrahydro-1-naphthalenyl)benzenesulfonamideD-Mannose 6-phosphateNonanoic acidNSI-189gamma-GlutamylcysteineSebacic acidFAHFA 16:1/17:2PG 4:0_20:5Nicotinamide adenine dinucleotide4-Hydroxymandelic acid11(Z),14(Z),17(Z)-Eicosatrienoic acid3',5,7-Trihydroxy-4'-methoxyflavanone3-Methyl-2-oxobutanoic acidPG O-18:1_14:1TheophyllineLPC 22:2D-(-)-FructoseLPE 18:42-Hydroxyvaleric acidAcetylenedicarboxylic acidAnacardic acidD-GlucarateLPG 17:0LPG 17:2LPG 17:1PG 2:0_18:1Ribulose-5-phosphatePC 14:0_18:2(+/-)9,10-dihydroxy-12Z-octadecenoic acidPhenothiazineDodecanedioic acidPC O-16:1_20:4D-Sedoheptulose 7-phosphateDeoxyguanosineQuinic acidLPE 18:0LPE 18:119(R)-hydroxy Prostaglandin A22,4-Dihydroxybenzoic acidLPE O-20:1LPE 18:3Choline bitartrateAdenosine 5'-monophosphateTrenbolone acetate2-(3,4-dimethoxyphenyl)ethanamineLPS 18:0LPS 18:1FAHFA 14:0/16:2ThymidinePC 13:0_15:0Orotic acidNicotinamide N-oxideMGDG O-28:1_20:5FAHFA 20:1/22:313,14-dihydro-15-keto-tetranor Prostaglandin D2Ne-(1-Carboxymethyl)-L-lysineInositolErythronolactoneCer 15:0;2O/14:0PC O-18:0_20:5Nonadecanoic acidcis-gondoic acid4-Hydroxy-L-Glutamic Acid13,14-dihydro-15-keto-tetranor Prostaglandin E2(R)-3-Hydroxy myristic acid20-Hydroxy-(5Z,8Z,11Z,14Z)-eicosatetraenoic acidMGDG O-20:0_20:518-¦Â-Glycyrrhetinic acidN-[2-(6-amino-9H-purin-9-yl)ethyl]-N-(2-furylmethyl)amineElaidic acidFAHFA 15:0/17:2FAHFA 2:0/16:0CynaropicrinLPG 19:1PhosphoethanolamineSL 17:0;O/16:0;OAzelaic acidLPG 19:0Cer 19:0;2O/13:1;(3OH)(FA 20:4)Cer 16:0;2O/16:0;(2OH)PG O-21:1_20:4LPG O-13:1ST 24:1;O4/18:2MGDG 15:0_16:02-{1-[2-(1-benzothiophen-5-ylamino)-2-oxoethyl]cyclohexyl}acetic acidS-LactoylglutathioneJWH 250 N-pentanoic acid metabolitePepstatinMGDG O-28:7_18:2BiopterinDaidzeinfalseMulti-omics analysis reveals the modulatory effects of Moringa oleifera ethanol extract on gut microbiota and metabolic profiles in broilers<p>The overuse of antimicrobial growth promoters (AGPs) in poultry farming has raised global concerns about antimicrobial resistance and food safety, prompting the search for effective natural alternatives. Moringa is rich in bioactive compounds with antimicrobial and immunomodulatory properties; however, its effects on the cecal microbiota and metabolome of broiler chickens when administered via drinking water remain unclear. This study employed 16S rDNA sequencing and non-targeted metabolomics to evaluate the effects of Moringa oleifera ethanol extract (MOE) and the broad-spectrum antibiotic bacitracin zinc (BZ) on the cecal microbiota, metabolite profiles, and their correlations in broiler chickens. The findings demonstrated MOE did not significantly affect α-diversity but altered β-diversity, resulting in a clear separation from the control and BZ groups. Linear discriminant analysis effect size (LEfSe) analysis identified Lachnospiraceae and Methanobacteriaceae as key differential biomarkers in the MOE group, whereas Deferribacterales was selectively enriched in the BZ group. At the phylum level, MOE enriched the Euryarchaeota, while BZ increased the abundance of potentially pathogenic phyla, including the Desulfobacterota and Proteobacteria. At the genus level, MOE promoted the growth of beneficial genera such as Faecalibacterium, Lachnoclostridium, and Alistipes, whereas BZ exhibited a bidirectional effect, increasing both pathogenic and beneficial groups. Metabolomic analysis revealed that MOE significantly regulated the metabolism of sphingolipids, tryptophan, and polyamines, as well as that of vitamin D3 and nicotinamide, whereas BZ primarily activated lipid metabolism and inhibited arginine-related antioxidant pathways. Correlation analysis further established genus-metabolite networks centered on Lachnoclostridium and Lactobacillus in the MOE group, and Alistipes and Collinsella in the BZ group. Overall, MOE is associated with alterations in cecal microbiota and host metabolism by enriching butyrate-producing bacteria and methanogenic archaea, accompanied by extensive metabolic reprogramming involving immune-related and antioxidant pathways. These findings support MOE as a promising natural alternative to antibiotics, highlighting its positive potential in regulating beneficial bacterial genera and metabolic pathways, and providing multi-omics evidence and a theoretical foundation for its use as a functional additive.</p>2026-08-042026-07-15MTBLS15049Com_5_posCom_6_posCom_12_posCom_14_posCom_17_posCom_24_posCom_52_posCom_57_posCom_93_posCom_103_posCom_136_posCom_146_posCom_168_posCom_179_posCom_222_posCom_311_posCom_334_posCom_433_posCom_436_posCom_454_posCom_471_posCom_481_posCom_550_posCom_1195_posCom_1396_posCom_1511_posCom_1639_posCom_1668_posCom_1688_posCom_1933_posCom_2202_posCom_2304_posCom_2763_posCom_2937_posCom_3070_posCom_3169_posCom_3197_posCom_3774_posCom_4165_posCom_4678_posCom_10356_posCom_23019_posCom_1_posCom_15_posCom_16_posCom_21_posCom_38_posCom_65_posCom_72_posCom_89_posCom_166_posCom_194_posCom_225_posCom_260_posCom_283_posCom_305_posCom_333_posCom_345_posCom_388_posCom_401_posCom_440_posCom_467_posCom_510_posCom_517_posCom_692_posCom_962_posCom_977_posCom_1037_posCom_1094_posCom_1527_posCom_1851_posCom_2716_posCom_3788_posCom_7907_posCom_13849_posCom_16055_posCom_2_posCom_4_posCom_13_posCom_25_posCom_29_posCom_34_posCom_36_posCom_42_posCom_50_posCom_61_posCom_70_posCom_71_posCom_98_posCom_106_posCom_122_posCom_127_posCom_135_posCom_153_posCom_164_posCom_180_posCom_191_posCom_196_posCom_206_posCom_255_posCom_265_posCom_276_posCom_282_posCom_288_posCom_293_posCom_295_posCom_297_posCom_310_posCom_315_posCom_320_posCom_327_posCom_335_posCom_361_posCom_362_posCom_379_posCom_389_posCom_406_posCom_407_posCom_412_posCom_427_posCom_438_posCom_448_posCom_480_posCom_487_posCom_502_posCom_505_posCom_509_posCom_512_posCom_514_posCom_521_posCom_534_posCom_538_posCom_546_posCom_551_posCom_572_posCom_592_posCom_595_posCom_612_posCom_622_posCom_669_posCom_679_posCom_680_posCom_684_posCom_696_posCom_697_posCom_733_posCom_744_posCom_746_posCom_765_posCom_778_posCom_793_posCom_794_posCom_796_posCom_809_posCom_812_posCom_828_posCom_854_posCom_874_posCom_895_posCom_897_posCom_925_posCom_935_posCom_959_posCom_960_posCom_999_posCom_1008_posCom_1027_posCom_1034_posCom_1045_posCom_1050_posCom_1051_posCom_1071_posCom_1086_posCom_1104_posCom_1111_posCom_1127_posCom_1139_posCom_1146_posCom_1151_posCom_1166_posCom_1191_posCom_1197_posCom_1204_posCom_1244_posCom_1272_posCom_1276_posCom_1277_posCom_1312_posCom_1360_posCom_1363_posCom_1377_posCom_1384_posCom_1392_posCom_1426_posCom_1434_posCom_1461_posCom_1486_posCom_1495_posCom_1526_posCom_1541_posCom_1542_posCom_1609_posCom_1614_posCom_1638_posCom_1660_posCom_1669_posCom_1675_posCom_1676_posCom_1698_posCom_1702_posCom_1724_posCom_1732_posCom_1739_posCom_1766_posCom_1780_posCom_1785_posCom_1789_posCom_1791_posCom_1838_posCom_1840_posCom_1848_posCom_1893_posCom_1894_posCom_1896_posCom_1921_posCom_1943_posCom_1985_posCom_1999_posCom_2053_posCom_2072_posCom_2091_posCom_2109_posCom_2121_posCom_2141_posCom_2151_posCom_2196_posCom_2233_posCom_2237_posCom_2238_posCom_2241_posCom_2259_posCom_2261_posCom_2274_posCom_2281_posCom_2323_posCom_2339_posCom_2340_posCom_2346_posCom_2378_posCom_2383_posCom_2425_posCom_2437_posCom_2440_posCom_2441_posCom_2457_posCom_2498_posCom_2510_posCom_2522_posCom_2524_posCom_2525_posCom_2530_posCom_2538_posCom_2542_posCom_2546_posCom_2584_posCom_2626_posCom_2646_posCom_2667_posCom_2685_posCom_2694_posCom_2699_posCom_2744_posCom_2754_posCom_2755_posCom_2757_posCom_2761_posCom_2765_posCom_2803_posCom_2816_posCom_2831_posCom_2872_posCom_2891_posCom_2896_posCom_2906_posCom_2915_posCom_2920_posCom_2953_posCom_2986_posCom_3011_posCom_3017_posCom_3020_posCom_3023_posCom_3035_posCom_3103_posCom_3117_posCom_3127_posCom_3134_posCom_3158_posCom_3177_posCom_3180_posCom_3183_posCom_3207_posCom_3213_posCom_3225_posCom_3228_posCom_3229_posCom_3231_posCom_3260_posCom_3262_posCom_3286_posCom_3296_posCom_3304_posCom_3327_posCom_3342_posCom_3361_posCom_3366_posCom_3371_posCom_3376_posCom_3383_posCom_3389_posCom_3393_posCom_3401_posCom_3416_posCom_3434_posCom_3455_posCom_3469_posCom_3513_posCom_3525_posCom_3530_posCom_3549_posCom_3568_posCom_3572_posCom_3589_posCom_3623_posCom_3652_posCom_3669_posCom_3687_posCom_3721_posCom_3730_posCom_3745_posCom_3752_posCom_3760_posCom_3769_posCom_3770_posCom_3811_posCom_3827_posCom_3893_posCom_3909_posCom_3942_posCom_3958_posCom_3969_posCom_3977_posCom_3984_posCom_4000_posCom_4011_posCom_4014_posCom_4031_posCom_4065_posCom_4116_posCom_4139_posCom_4174_posCom_4176_posCom_4210_posCom_4222_posCom_4226_posCom_4240_posCom_4246_posCom_4260_posCom_4293_posCom_4295_posCom_4325_posCom_4331_posCom_4341_posCom_4343_posCom_4346_posCom_4375_posCom_4379_posCom_4417_posCom_4432_posCom_4438_posCom_4447_posCom_4451_posCom_4473_posCom_4494_posCom_4540_posCom_4544_posCom_4548_posCom_4569_posCom_4615_posCom_4617_posCom_4625_posCom_4718_posCom_4733_posCom_4775_posCom_4789_posCom_4827_posCom_4831_posCom_4839_posCom_4845_posCom_4875_posCom_4881_posCom_4903_posCom_4911_posCom_4944_posCom_4957_posCom_4970_posCom_4999_posCom_5035_posCom_5069_posCom_5070_posCom_5077_posCom_5097_posCom_5124_posCom_5149_posCom_5172_posCom_5175_posCom_5185_posCom_5191_posCom_5273_posCom_5333_posCom_5349_posCom_5364_posCom_5447_posCom_5452_posCom_5458_posCom_5477_posCom_5495_posC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