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The preprocessing workflow included baseline filtering, peak detection, peak integration, retention-time correction, and peak alignment. A data matrix containing retention time, mass-to-charge ratio (m/z), and peak intensity was subsequently generated.</p><p><br></p><p>Metabolite annotation was performed by matching the acquired MS and MS/MS spectral information against public metabolite databases, including the Human Metabolome Database (HMDB) and METLIN, as well as a proprietary in-house spectral library. Metabolite identities were assigned based on the combined matching results.</p>"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - hilic","Liquid Chromatography MS - negative - hilic"],"chromatography_protocol":["<p>A 3 μL aliquot of each sample was injected into an ultrahigh-performance liquid chromatography system and separated on an ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm i.d., 1.8 μm). Mobile phase A consisted of water/acetonitrile (95:5, v/v) containing 0.1% formic acid, while mobile phase B consisted of acetonitrile/isopropanol/water (47.5:47.5:5, v/v/v) containing 0.1% formic acid. The flow rate was maintained at 0.40 mL/min, and the column temperature was set at 40°C.</p>"],"publication":["Metabolites in the cecal contents of blast-injured mice."],"submitter_name":["Sijie Wang"],"submitter_affiliation":["Northwestern Polytechnical University"],"organism_part":["cecels content"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["<p>Approximately 50 mg of each solid sample was transferred into a 2 mL centrifuge tube containing a 6 mm-diameter grinding bead. Subsequently, 400 μL of extraction solvent consisting of methanol and water (4:1, v/v) and containing 0.02 mg/mL L-2-chlorophenylalanine as the internal standard was added.</p><p><br></p><p>The samples were homogenized using a cryogenic tissue grinder for 6 min at 10°C and 50 Hz, followed by ultrasonic extraction for 30 min at 5°C and 40 kHz. The samples were then incubated at 20°C for 30 min and centrifuged at 13,000 × g for 15 min at 4°C. The resulting supernatant was transferred into autosampler vials equipped with glass inserts for LC–MS analysis.</p>"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15071"],"author":["sijie wang. Northwestern Polytechnical University. 923068721@qq.com."],"data_transformation_protocol":["<p>Data were acquired in data-dependent acquisition mode. Stepped normalized collision energies of 20, 40, and 60 were applied for tandem mass spectrometric fragmentation. The resolutions of the full MS and MS/MS scans were set to 60,000 and 7,500, respectively.</p>"],"study_factor":["Group"],"submitter_email":["sijie@mail.nwpu.edu.cn"],"sample_collection_protocol":["<p>Metabolites in the cecal contents of blast-injured mice</p>"],"omics_type":["Metabolomics"],"study_design":["20-HETE","Metabolomics","Mus musculus","Traumatic brain injury","untargeted analysis","Limosilactobacillus reuteri","Thermo Scientific Q Exactive HF-X","cecal content","UHPLC","cecels content","gut-brain axis","anxious-like behavior"],"curator_keywords":["20-HETE","Metabolomics","Mus musculus","Traumatic brain injury","untargeted analysis","Limosilactobacillus reuteri","Thermo Scientific Q Exactive HF-X","cecal content","UHPLC","cecels content","gut-brain axis","anxious-like behavior"],"mass_spectrometry_protocol":["<p>Mass spectrometric detection was performed in both positive and negative electrospray ionization modes. Full-scan mass spectra were acquired over an m/z range of 70–1050. The sheath gas pressure was set to 50 psi, and the auxiliary gas pressure was set to 13 psi. The auxiliary gas heater temperature and ion transfer tube temperature were maintained at 425°C and 325°C, respectively. The spray voltages were set to 3.5 kV in positive-ion mode and 3.5 kV in negative-ion mode.</p>"],"metabolite_name":["Taurine","6beta-Hydroxyasiatic acid","Genistin","Allantoin","9,10-DHOME","Hydrocinnamic acid","p-Cresol sulfate","Lipoxin B4","Riboflavin","5-Thymidylic acid","3,3-Dimethylglutaric acid","Pyrocatechol","D-2,3-Dihydroxypropanoic acid","Lithocholic acid","Hypogeic acid","D-Xylose","gamma-Aminobutyric acid","Deoxyinosine","LysoPA(16:0/0:0)","1,11-Undecanedicarboxylic acid","2-Hydroxyethanesulfonate","Deoxyribose 1-phosphate","N-Acetylvaline","D-Alanyl-D-alanine","L-Erythrulose","Kynurenic acid","Ketoleucine","L-Allothreonine","2-(3,4-dihydroxyphenyl)-3,5-dihydroxy-7-methoxy-4H-chromen-4-one","2-Methylbenzoic acid","Myristoleic acid","Isoliquiritigenin","Pyridoxal","Picrotin","Lipoxin A4","Carboxytolbutamide","Oxoglutaric acid","2,3-Dinor-TXB2","Biotin","Citrulline","[4]-Gingerdiol 3,5-diacetate","Porphobilinogen","Paxilline","Alanylglycine","1-Kestose","L-Proline","Hydroxypyruvic acid","Phenylalanylproline","D-Glucuronic acid","Aldehydo-D-xylose","Syringic acid","(R)-mandelic Acid","Mesaconic acid","4-Hydroxyproline","Ricinoleic acid","Ethyl glucuronide","Rhapontigenin","3-Sulfinato-L-alaninate","alpha-Tocopherol","Pyruvic acid","1,2,3-Trihydroxybenzene","Prostaglandin E3","Butyrylcarnitine","D-Mannose","D-Glutamine","N6-Acetyl-L-lysine","Coumesterol","Maltitol","Gentisic acid","L-Norleucine","Oleic acid","d-Tocotrienol","Glutaric acid","[12]-Gingerol","2'-O-Methyladenosine","Epsilon-(gamma-Glutamyl)-lysine","Pentadecanoic acid","N-Acetylglutamic acid","cis-Vaccenic acid","Deoxyuridine","Kaempferol","Dihydrolipoate","Galactinol","Pseudouridine","(1R,2S,3R)-2-Acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)imidazole","Glycitin","Prolylhydroxyproline","Zeranol","Estrone glucuronide","Isoferulic acid","Indole-3-propionic acid","2-Ethyl-2-Hydroxybutyric acid","L-Valine","Uracil","N-Acetylneuraminic acid","2-Oxo-4-methylthiobutanoic acid","Pantothenic acid","4-Pyridoxic acid","Indolelactic acid","1H-Indole-3-carboxaldehyde","Diethylphosphate","Guanosine","Sinapyl alcohol","N-Acetyl-L-methionine","Ribothymidine","Glyceraldehyde","Sinapic acid","beta-D-Glucosamine","Shikimic acid","16-Hydroxy hexadecanoic acid","Saccharin","Caprylic acid","Indoleacetaldehyde","4-Dodecylbenzenesulfonic Acid","Acetylglycine","Indoleacrylic acid","Glyceric acid","Dihydrojasmonic acid","Fructose-1P","Glycyl-Isoleucine","Hexadecanedioic acid","Crustecdysone","Steviobioside","D-Leucic acid","Phthalic acid","1-deoxy-1-(N6-lysino)-D-fructose","3-(3-Hydroxyphenyl)propanoic acid","Homoveratric acid","Cyclic AMP","Deoxyribose 5-phosphate","Dimethylmalonic acid","Alpha-Hydroxyisobutyric acid","L-Malic acid","Gibberellin A53","Acetylvalerenolic acid","Myristic acid","benzene-1,2,4-triol","Rhamnose","Uridine","Succinic acid","Hypoxanthine","L-Homocysteic acid","p-Anisic acid","Chenodeoxycholic acid","Enterodiol","Linamarin","3-Hydroxybenzoic acid","2-Keto-3-deoxy-D-gluconic acid","Malonic acid","Hippuric acid","2-Oxovaleric acid","Equol","3-Hydroxypicolinic acid","Prostaglandin A2","3-Aminoisobutanoic acid","3-Hydroxymethylglutaric acid","Homovanillic acid","Homocarnosine","L-Cysteine","6-Keto-prostaglandin F1a","(9xi,10xi,12xi)-9,10-Dihydroxy-12-octadecenoic acid","L-Lactic acid","3-Nitrotyrosine","o-Cresol","L-Glutamic acid","Adenosine","4-Ipomeanol","Benazeprilat","6-Methyladenine","Adenine","Prostaglandin B1","Uric acid","Oxoadipic acid","Prostaglandin B2","Macrocarpal B","Cortisone","Linoleic acid","Isobutyric acid","L-Tyrosine","Palmitoleic acid","(10E,12Z)-(9S)-9-Hydroperoxyoctadeca-10,12-dienoic acid","Citraconic acid","L-Serine","Inosine","3-Hydroxybutyric acid","3-Hydroxyphenylacetic acid","20-Carboxy-leukotriene B4","Phenylpyruvic acid","Ascorbic acid","L-Phenylalanine","Phenylacetic acid","Sucrose","Diacetone alcohol","Stearidonic acid","(S,E)-Zearalenone","Methylsuccinic acid","3,4-Dihydroxybenzeneacetic acid","Tetradecanedioic acid","Beta-D-Galactose","Traumatic acid","Cholic acid","D-Maltose","Arachidonic acid","3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid","8-Isoprostaglandin E1","LysoPE(18:1(9Z)/0:0)","Sphingosine","Isokobusone","4',5,7-Trihydroxy-6-prenylflavanone","Taurocholic acid","Allocholic acid","Terephthalic acid","2-Ketobutyric acid","Vanillylmandelic acid","Urocanic acid","Sebacic acid","11-Dehydro-thromboxane B2","Valeric acid","L-Iditol","Acetaminophen","Melezitose","N-Formyl-L-aspartate","2-Hydroxystearic acid","L-Gulonolactone","N-Acetylhistamine","N-Glycolylneuraminic acid","D-Malic acid","1-Aminocyclopropanecarboxylic acid","Bovinic acid","9,10-epoxyoctadecanoic acid","Succinic anhydride","Suberic acid","3-Methoxybenzenepropanoic acid","N-Acetylornithine","N-methyl-L-glutamic Acid","Guanine","Esculentic acid (Diplazium)","Levoglucosan","Succinic acid semialdehyde","myo-Inositol","Indoleacetic acid","N-Acetylserine","Mannitol","Tropate","Pyroglutamic acid","D-Xylulose","D-Aspartic acid","8,9-DiHETrE","Hydroxyphenyllactic acid","Eriodictyol","N-Acetylhistidine","2-Methylguanosine","Ascorbyl palmitate","Dodecanedioic acid","But-2-enoic acid","Xanthosine","Linoelaidic acid","Pyridoxine","Xanthine","3-Methyl-2-oxovaleric acid","1H-Indole-2,3-dione","Citric acid","Emodin","N-Acetyl-glucosamine 1-phosphate","7-Methylguanine","trans-Ferulic acid","Deoxyguanosine","5-Aminopentanoic acid","Gingerol","Quinic acid","N-Acetylleucine","Aesculin","N-Acetyl-L-alanine","4-Hydroxystyrene","xi-2,3-Dihydro-2-oxo-1H-indole-3-acetic acid","Nicotinic acid","Geranylgeranyl-PP","N-Acetylmuramate","Tiglic acid","Medicagenic acid","Cortisol","Thymidine","Tauroursodeoxycholic acid","Bilirubin","Glycocholic acid","L-Isoleucine","3-Hydroxysebacic acid","D-Alanine","Abscisic acid","Stearic acid","Stachyose","4-Acetamidobutanoic acid","7-Methylxanthine","Indoxyl sulfate","2-Hydroxy-3-methylbutyric acid","Adrenochrome","Cytidine","Dehydroascorbic acid","N-Acetyl-L-aspartic acid","15-Methylpalmitate","L-Lysine","Byssochlamic acid","5'-Deoxyadenosine","Phenoxyacetic acid","Sedoheptulose","9,10-Epoxyoctadecenoic acid","N-Alpha-acetyllysine","1-Methyl-6-phenyl-1H-imidazo[4,5-b]pyridin-2-amine","Cohumulone","Genistein","1,5-Anhydrosorbitol","Isolithocholic acid","20-Hydroxy-leukotriene B4","3,4-Dihydroxyhydrocinnamic acid","Pyrrolidonecarboxylic acid","L-3-Phenyllactic acid","3-Carboxy-4-methyl-5-propyl-2-furanpropionic acid","Epicatechin","4-Hydroxyphenylpyruvic acid","Genipin","Citramalic acid","3-(3,4-Dihydroxy-5-methoxy)-2-propenoic acid","2,3-Dihydroxybutanedioic acid","p-Cresol","15-Keto-prostaglandin E2","Azelaic acid","Perillic acid","Hydrogen phosphate","D-Ornithine","2-Hydroxymyristic acid","Thymine","Phosphocreatine","Aspalathin","Pelargonic acid","Deoxycholic acid","Parabanic Acid","Cyclohexanecarboxylic acid","Indole-3-carboxylic acid","3b-Hydroxy-5-cholenoic acid","Deoxycytidine","Calcidiol","D-Ribose","Mesalazine","5a-Tetrahydrocorticosterone","3,5,7-trihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-4-one","[6]-Gingerdiol 3,5-diacetate","Isonicotinic acid"],"additional_accession":[]},"is_claimable":false,"name":"Metabolites in the cecal contents of blast-injured mice","description":"<p>Metabolites in the cecal contents of blast-injured mice</p><p>A gut microbiota–20-HETE lipid signaling axis drives neuroinflammation and anxiety after TBI.</p><p>A gut microbiota–20-HETE lipid signaling axis drives neuroinflammation and anxiety after TBI.</p>","dates":{"publication":"2026-07-17","submission":"2026-07-17"},"accession":"MTBLS15071","cross_references":{"HMDB":["HMDB0038394","HMDB0000034","HMDB0000055","HMDB0000157","HMDB0001979","HMDB0003411","HMDB0034301","HMDB0012497","HMDB0000699","HMDB0000195","HMDB0000562","HMDB0033172","HMDB0032392","HMDB0029880","HMDB0011600","HMDB0060665","HMDB0039047","HMDB0003012","HMDB0003217","HMDB0000062","HMDB0003312","HMDB0001488","HMDB0031641","HMDB0000085","HMDB0000014","HMDB0000883","HMDB0011749","HMDB0000097","HMDB0036105","HMDB0041441","HMDB0000064","HMDB0029576","HMDB0000630","HMDB0002802","HMDB0031721","HMDB0041570","HMDB0001406","HMDB0000071","HMDB0013854","HMDB0041451","HMDB0040638","HMDB0035396","HMDB0013751","HMDB0036057","HMDB0040518","HMDB0038322","HMDB0060475","HMDB0029806","HMDB0001888","HMDB0000235","HMDB0000043","HMDB0000089","HMDB0000619","HMDB0062251","HMDB0006831","HMDB0000177","HMDB0037111","HMDB0000870","HMDB0011729","HMDB0004101","HMDB0031459","HMDB0000805","HMDB0000301","HMDB0000222","HMDB0031635","HMDB0000696","HMDB0029409","HMDB0004094","HMDB0011599","HMDB0033112","HMDB0036434","HMDB0036777","HMDB0003355","HMDB0030057","HMDB0002039","HMDB0039040","HMDB0002117","HMDB0010340","HMDB0014291","HMDB0000897","HMDB0000626","HMDB0001366","HMDB0004827","HMDB0000148","HMDB0034244","HMDB0000292","HMDB0000050","HMDB0014389","HMDB0000875","HMDB0033529","HMDB0004816","HMDB0000975","HMDB0000925","HMDB0028951","HMDB0004339","HMDB0002134","HMDB0060852","HMDB0001891","HMDB0012897","HMDB0004437","HMDB0000296","HMDB0005781","HMDB0001565","HMDB0002064","HMDB0059643","HMDB0000210","HMDB0000101","HMDB0039998","HMDB0002820","HMDB0029007","HMDB0038918","HMDB0029579","HMDB0029391","HMDB0001563","HMDB0003539","HMDB0034732","HMDB0038953","HMDB0002339","HMDB0036382","HMDB0011718","HMDB0013716","HMDB0032914","HMDB0037550","HMDB0033835","HMDB0001547","HMDB0034617","HMDB0011504","HMDB0000133","HMDB0001173","HMDB0011503","HMDB0002098","HMDB0032358","HMDB0036174","HMDB0028757","HMDB0000239","HMDB0060428","HMDB0031210","HMDB0006236","HMDB0011502","HMDB0005765","HMDB0033034","HMDB0060436","HMDB0033598","HMDB0004077","HMDB0031472","HMDB0000824","HMDB0060309","HMDB0040173","HMDB0000244","HMDB0003361","HMDB0000738","HMDB0032299","HMDB0000905","HMDB0028691","HMDB0000403","HMDB0035514","HMDB0041440","HMDB0013222","HMDB0000904","HMDB0001414","HMDB0029435","HMDB0001526","HMDB0038831","HMDB0000056","HMDB0000162","HMDB0013815","HMDB0000251","HMDB0012219","HMDB0000300","HMDB0029132","HMDB0003331","HMDB0036739","HMDB0039064","HMDB0061007","HMDB0000991","HMDB0000241","HMDB0035983","HMDB0029076","HMDB0006695","HMDB0036863","HMDB0031244","HMDB0032231","HMDB0028934","HMDB0031667","HMDB0031811","HMDB0000030","HMDB0029665","HMDB0004234","HMDB0037296","HMDB0001903","HMDB0032015","HMDB0000517","HMDB0038917","HMDB0002022","HMDB0012296","HMDB0001539","HMDB0060990","HMDB0011480","HMDB0040992","HMDB0006059","HMDB0001276","HMDB0034104","HMDB0000842","HMDB0031346","HMDB0004242","HMDB0028988","HMDB0000086","HMDB0001084","HMDB0030339","HMDB0011484","HMDB0029011","HMDB0029139","HMDB0038568","HMDB0033464","HMDB0004670","HMDB0001189","HMDB0033699","HMDB0002224","HMDB0031850","HMDB0059611","HMDB0062312","HMDB0060681","HMDB0013655","HMDB0002369","HMDB0039531","HMDB0039229","HMDB0001431","HMDB0000159","HMDB0035196","HMDB0031934","HMDB0062222","HMDB0011177","HMDB0006029","HMDB0001902","HMDB0000017","HMDB0011181","HMDB0060427","HMDB0036859","HMDB0001310","HMDB0061650","HMDB0000644","HMDB0031661","HMDB0032568","HMDB0001119","HMDB0030844","HMDB0000252","HMDB0028700","HMDB0039839","HMDB0034203","HMDB0002284","HMDB0012176","HMDB0001248","HMDB0032853","HMDB0040021","HMDB0032985","HMDB0000182","HMDB0028914","HMDB0031581","HMDB0040054","HMDB0030272","HMDB0142894","HMDB0000214","HMDB0002972","HMDB0000641","HMDB0029427","HMDB0006115","HMDB0028721","HMDB0001008","HMDB0040592","HMDB0011144","HMDB0059676","HMDB0028955","HMDB0012815","HMDB0028998","HMDB0004370","HMDB0040700","HMDB0006247","HMDB0030041","HMDB0029636","HMDB0001301","HMDB0028989","HMDB0039982","HMDB0000267","HMDB0029001","HMDB0000259","HMDB0041838","HMDB0059651","HMDB0038289","HMDB0034252","HMDB0034588","HMDB0011478","HMDB0039238","HMDB0001961","HMDB0036313","HMDB0000194","HMDB0038249","HMDB0059683","HMDB0033944","HMDB0032210","HMDB0003553","HMDB0031173","HMDB0000866","HMDB0011742","HMDB0006535","HMDB0041371","HMDB0059696","HMDB0028963","HMDB0029440","HMDB0002894","HMDB0035687","HMDB0031308","HMDB0040284","HMDB0032034","HMDB0012880","HMDB0012252","HMDB0029207","HMDB0007003","HMDB0041824","HMDB0032111","HMDB0000269","HMDB0002013","HMDB0037510","HMDB0001495","HMDB0032229","HMDB0039311","HMDB0034887","HMDB0030905","HMDB0038824","HMDB0062618","HMDB0031861","HMDB0002725","HMDB0029004","HMDB0032224","HMDB0033926","HMDB0060666","HMDB0038140","HMDB0038516","HMDB0035665","HMDB0002273","HMDB0032090","HMDB0034755","HMDB0038239","HMDB0033527","HMDB0029136","HMDB0006710","HMDB0032969","HMDB0038628","HMDB0041728","H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