Metabolite identification was carried out by matching retention times and MS/MS fragmentation patterns against an in-house authentic standard library. Each amino acid was annotated based on exact mass, characteristic fragment ions, and co-elution with corresponding reference standards. Peak integration, calibration, and quantification were performed using vendor-provided quantitative analysis software. Data preprocessing, including peak alignment and quality control assessment, was conducted using widely adopted metabolomics platforms. Metabolite annotations were further cross-referenced with public databases including the Human Metabolome Database (HMDB), Kyoto Encyclopedia of Genes and Genomes (KEGG), and METLIN, where applicable.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase"],"chromatography_protocol":["The UHPLC separation was carried out using Thermo vanquish UHPLC System (Thermo Fisher), equipped with a Waters ACQUITY UPLC BEH C18 (100×2.1 mm, 1.7 μm). The mobile phase A was 5 mM ammonium acetate in water, and the mobile phase B was acetonitrile. The column temperature was set at 45 °C. The auto-sampler temperature was set at 4 °C and the injection volume was 2 μL.
"],"publication":["Integrative Ophthalmology of Traditional Chinese and Western Medicine."],"submitter_name":["Zefeng Kang"],"submitter_affiliation":["China Academy of Chinese Medical Sciences Eye Hospital"],"organism_part":["Serum"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["1. Thaw the sample in an ice water bath and vortex for 30 seconds before taking the sample;
2. Use a pipette to accurately take 15 μL of the sample into a 1.5 mL EP tube, add 35 μL of pure water and 200 μL of extraction solution (acetonitrile:methanol, 1:1 ratio, containing isotope internal standard mixture, pre-cooled at -40℃), vortex for 30 seconds;
3. Sonicate in an ice water bath for 15 minutes;
4. Let the sample stand at -40℃ for 1 hour;
5. Centrifuge the sample at 4℃, 12,000 rpm (13800×g, radius 8.6 cm) for 15 minutes;
6. Take 100 μL of the supernatant and evaporate to dryness; re-dissolve with 100 μL 50% methanol in water, add 100 μL derivatization reagent, add 50 μL 1 M NaHCO3, vortex; derivatize in a 40℃ water bath for 1 hour, let it cool to room temperature, add 50 μL 2 M HCl, evaporate to dryness; re-dissolve in 200 μL methanol and submit for analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15095"],"author":["Zefeng Kang. China Academy of Chinese Medical Sciences Eye Hospital. kangzf7717@126.com."],"data_transformation_protocol":["The raw data was converted to mzML format using ProteoWizard msconvert.
"],"study_factor":["Disease"],"submitter_email":["kangzf7717@126.com"],"sample_collection_protocol":["Mouse serum samples were collected from 18 mice, comprising a disease group (n=9) and a control group (n=9). All samples were immediately stored at −80°C upon receipt and maintained at this temperature until targeted amino acid analysis was performed. No additional pretreatment or processing was applied prior to storage. Samples from both groups were analyzed simultaneously to minimize batch effects.
"],"omics_type":["Metabolomics"],"study_design":["normal","Metabolomics","Vanquish","Mus musculus","untargeted analysis","myopia","MRM","Serum","ESI","experimental blank","TSQ Altis Plus"],"curator_keywords":["normal","Metabolomics","Vanquish","Mus musculus","untargeted analysis","myopia","MRM","Serum","ESI","experimental blank","TSQ Altis Plus"],"mass_spectrometry_protocol":["Thermo Altis TSQ Plus Mass Spectrometer (Thermo Fisher, USA), equipped with an electrospray ionization interface, was applied for assay development. Typical ion source parameters were: Spray Voltage = -3300 V, Sheath Gas = 40 Arb, Aux Gas = 10 Arb, Sweep Gas = 1 Arb, Ion Transfer Tube Temp = 325 °C , Vaporizer Temp = 350 °C. The MRM parameters for each of the targeted analytes were optimized using flow injection analysis, by injecting the standard solutions of the individual analytes, into the API source of the mass spectrometer. Several most sensitive transitions were used in the MRM scan mode to optimize the collision energy for each Q1/Q3 pair. Among the optimized MRM transitions per analyte, the Q1/Q3 pairs that showed the highest sensitivity and selectivity were selected as ‘quantifier’ for quantitative monitoring. The additional transitions acted as 'qualifier' for the purpose of verifying the identity of the target analytes.
"],"additional_accession":[]},"is_claimable":false,"name":"Integrative Ophthalmology of Traditional Chinese and Western Medicine","description":"Research directions include prevention and control of myopia, intervention against age-related macular degeneration, ophthalmic optical research, and research on ophthalmology-oriented artificial intelligence technologies.","dates":{"publication":"2026-07-21","submission":"2026-07-20"},"accession":"MTBLS15095","cross_references":{}}