MetaboLights

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identification was performed based on the accuracy of precursor m/z values and MS/MS fragmentation patterns. The merged dataset was imported into the R package ropls (version 1.30.0+) for orthogonal partial least squares discriminant analysis (OPLS-DA). Metabolites were annotated by matching the experimental m/z values and tandem mass spectra against the KEGG database (accessed March 2024). Compounds were considered putatively identified when the mass accuracy was within acceptable tolerance and MS/MS spectral matching was confirmed. Differentially accumulated metabolites were selected based on VIP (Variable Importance in Projection) values > 1.0 and P-value < 0.05. Pathway enrichment analysis was subsequently performed using KEGG pathway mapping.MetaboLightsPublicLiquid Chromatography MS - positive - hilicLiquid Chromatography MS - negative - hilicBrain tissues were collected from juvenile Eleutheronema tetradactylum at three time points: Day 0 (control group, 28 °C), Day 7, and Day 14 post-exposure to low-temperature stress (18 °C). Three fish were randomly selected from each tank at each time point (18 fish in total per time point). Three brain tissue samples were fixed in 4% paraformaldehyde for histological analysis. The remaining brain tissues were immediately snap-frozen in liquid nitrogen and stored at −80 °C until further metabolomic analysis.Low-temperature induced neuro-oxidative stress and metabolic reprogramming in the brain of juvenile fourfinger threadfin (Eleutheronema tetradactylum).Guangdong Ocean UniversityShuiPing Hebrainmass spectrometry assayBrain tissues were collected from juvenile Eleutheronema tetradactylum at three time points: Day 0 (control group, 28 °C), Day 7, and Day 14 post-exposure to low-temperature stress (18 °C). Three fish were randomly selected from each tank at each time point (18 fish in total per time point). Three brain tissue samples were fixed in 4% paraformaldehyde for histological analysis. The remaining brain tissues were immediately snap-frozen in liquid nitrogen and stored at −80 °C until further metabolomic analysis.Elentheronema tetradactylumhttps://www.ebi.ac.uk/metabolights/MTBLS15103ZhongLiang Wang. Guangdong Ocean University. wangzl@gdou.edu.cn.Raw mass spectrometry data were converted to mzXML files using the ProteoWizard MSConvert tool. The converted files were then imported into XCMS software (v3.18.0) for peak detection, alignment, and retention time correction. Peak selection parameters were set as follows: centWave m/z = 10 ppm, peak width = c(10, 60), and pre-filter = c(10, 100). Peak grouping parameters included bw = 5, mzwid = 0.025, and minfrac = 0.5. Isotope and adduct annotation were performed using the CAMERA (Collection of Algorithms of Metabolite pRofile Annotation) R package. Only features with non-zero measurements in at least one group exceeding 50% were retained. After processing, positive and negative ion data were merged into a combined dataset for subsequent statistical analysis.Exposure-timeLow temperaturehexinyu@stu.gdou.edu.cnBrain tissues were collected from juvenile Eleutheronema tetradactylum at three time points: Day 0 (control group, 28 °C), Day 7, and Day 14 post-exposure to low-temperature stress (18 °C). Three fish were randomly selected from each tank at each time point (18 fish in total per time point). Three brain tissue samples were fixed in 4% paraformaldehyde for histological analysis. The remaining brain tissues were immediately snap-frozen in liquid nitrogen and stored at −80 °C until further metabolomic analysis.MetabolomicsMetabolomicslow-temperature stressuntargeted analysisneuro-oxidative stressMetabolic ReprogrammingEleutheronema tetradactylumbrainAgilent 1290 Infinity LCAB SCIEX TripleTOF 6600Elentheronema tetradactylumexperimental sampleMetabolomicslow-temperature stressuntargeted analysisneuro-oxidative stressMetabolic ReprogrammingEleutheronema tetradactylumbrainAgilent 1290 Infinity LCAB SCIEX TripleTOF 6600Elentheronema tetradactylumexperimental sampleBrain tissues were collected from juvenile Eleutheronema tetradactylum at three time points: Day 0 (control group, 28 °C), Day 7, and Day 14 post-exposure to low-temperature stress (18 °C). Three fish were randomly selected from each tank at each time point (18 fish in total per time point). Three brain tissue samples were fixed in 4% paraformaldehyde for histological analysis. The remaining brain tissues were immediately snap-frozen in liquid nitrogen and stored at −80 °C until further metabolomic analysis.falseLow-temperature induced neuro-oxidative stress and metabolic reprogramming in the brain of juvenile fourfinger threadfin (Eleutheronema tetradactylum)This study investigated the effects of low-temperature stress on the brain metabolome of juvenile fourfinger threadfin (Eleutheronema tetradactylum). Using UHPLC-Q-TOF-MS-based untargeted metabolomics, we analyzed brain tissues from fish exposed to 28°C (control) and 18°C for 7 and 14 days. The aim was to identify key metabolic pathways and neuro-oxidative stress responses under cold stress, providing insights into the physiological adaptation mechanisms of this species to low temperature.2026-07-222026-07-20MTBLS15103