MetaboLights

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Identification confidence levels follow the Metabolomics Standards Initiative (MSI) reporting guidelines. Differential metabolites were defined based on variable importance in projection (VIP) greater than 1, fold change greater than 1.2 or less than 0.833, and p-value less than 0.05.MetaboLightsPublicLiquid Chromatography MS - positive - hilicLiquid Chromatography MS - negative - hilicChromatographic separation was performed on a Vanquish UHPLC system (Thermo Fisher Scientific) using a Waters ACQUITY UPLC BEH Amide column (2.1 x 50 mm, 1.7 um) for HILIC separation. Mobile phase A was 25 mmol/L ammonium acetate and 25 mmol/L ammonium hydroxide in water (pH 9.75). Mobile phase B was acetonitrile. The gradient elution was performed at a flow rate of 0.5 mL/min. Column temperature was maintained at 45 degrees C. Injection volume was 2 uL.Serum Metabolomics Reveals Stage-Dependent Metabolic Transitions During CIDR-PGF2alpha-Induced Estrus in Kazak Ewes. 10.3389/fvets.2026.1904685.Yaseen UllahShihezi Universityserummass spectrometry assaySerum samples were thawed at 4 degrees C and 100 uL of each sample was mixed with 400 uL of methanol/acetonitrile (1:1, v/v) containing internal standards. Samples were vortexed for 30 s, incubated at -20 degrees C for 30 min, then centrifuged at 14000 rpm for 20 min at 4 degrees C. The supernatant was transferred and dried under nitrogen. Residues were reconstituted in 100 uL acetonitrile/water (1:1, v/v). Pooled quality control (QC) samples were prepared by mixing equal volumes of all individual samples.date this protocol descriptionOvis arieshttps://www.ebi.ac.uk/metabolights/MTBLS15117Zhao Zongsheng. Shihezi University. zhaozongsh@shzu.edu.Yaseen Ullah. Shihezi University. hbksons@outlook.com.Raw data files were converted from instrument format to mzXML format using ProteoWizard MSConvert (v3.0.24054). Peak detection, alignment and quantification were performed by the metabolomics analysis company (Wuhan Berna Technology Co., Ltd). TIC normalization was applied to all samples prior to statistical analysis.Reproductive stagehbksons@outlook.comBlood samples were collected from 18 Kazak ewes (6 per group) at defined reproductive stages following CIDR-PGF2alpha synchronization treatment. Serum was separated by centrifugation at 3000 rpm for 10 min at 4 degrees C and stored at -80 degrees C until analysis.e this protocol descriptionMetabolomicsVanquish UHPLChydrophilic interaction chromatographyProteoWizard msconvertMetabolomicsuntargeted analysisnegative ion modeReproductive PhysiologySIMCAquality control sampleOrbitrap Exploris 120CIDR-PGF2alphaelectrospray ionizationexperimental sampleOvis ariesEstrus SynchronizationUHPLC-MS/MSKazak ewesXCMSWuhan Berna Technology Co., LtdserumVanquish UHPLChydrophilic interaction chromatographyProteoWizard msconvertMetabolomicsuntargeted analysisnegative ion modeReproductive PhysiologySIMCAquality control sampleOrbitrap Exploris 120CIDR-PGF2alphaelectrospray ionizationexperimental sampleOvis ariesEstrus SynchronizationUHPLC-MS/MSKazak ewesXCMSWuhan Berna Technology Co., LtdserumMass spectrometric detection was performed on an Orbitrap Exploris 120 mass spectrometer (Thermo Fisher Scientific) equipped with an electrospray ionization (ESI) source. Data were acquired in both positive and negative ion modes. ESI voltage was set to +3.8 kV in positive mode and -3.4 kV in negative mode. The scan m/z range was 70-1050. Data were collected using the Orbitrap mass analyzer at high resolution.Benzoic acidDihydroxyacetoneEmbelin1,6-Anhydro-.beta.-D-glucoseLeu-IleTraumatic acid3-Hydroxyglutaric acidGlycolithocholic acid 3-sulfateDodecyl gallateAla-GlySunitinib5-Guanidino-2-oxopentanoate2-Ketocaproic acidSuccinic_acid_semialdehydeKetoleucinePhe-TrpPhe-Met-Arg-Phe-amide4,5-DIDEMETHYLSIMMONDSINalpha-Ketoisovaleric acidCapric acidSer-AlaLawsone(3-Methoxy-4-hydroxyphenyl)ethylene_glycol_sulfate(2E)-4-Hydroxybut-2-enoic acidRoyal jelly acidArabinono-1,4-lactonePE(18:0/18:2)Val-IleGentisic acidmyo-InositolPregnanediol 3-O-glucuronideIndoleacetic acidPhosphate2,3-Dihydroxybenzoic acid (Pyrocatechuic acid)2,6-Dihydroxybenzoic acidThyroxine17.beta.-Estradiol 3-.beta.-D-glucuronideHydroxyphenyllactic acidGlaucarubinN-LactoylphenylalanineTaurolithocholic acidSalicylic acidPiperonylic acid3-Methyl-2-oxovaleric acidXanthineEthoxyacetic acidGlycylleucinePhloroglucinaldehydeLeu-Trp2,4-Dihydroxybenzoic acid(10E,15Z)-9,12,13-Trihydroxyoctadeca-10,15-dienoic acidSyringic_acidUracilHygric acidLactatecis-9,10-Epoxystearic acid1-Palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoserine5-Hydroxytryptophol (5HTOL)(2-aminoethoxy)[2-[icosa-5.8.11.14-tetraenoyloxy]-3-[octadec-11-enoyloxy]propoxy]phosphinic acidLeu-Val9-Oxo-10(E),12(E)-octadecadienoic acid3-Methoxy-4-hydroxyphenylglycol sulfateLeu-LeuEthanesulfonic acidMalic acidDaminozideIndoxyl sulfateIle-LeuMupirocin2-Arachidonoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamineChicoric acidGlutamate2-FuroylglycineOxalic acid5-Sulfosalicylic acidHydroxymalonateOxypurinolPyruvaldehydeostholarctiincis-11.14-Eicosadienoic acidAlanylalanine (Ala-Ala)1-PalmitoyllysophosphatidateLPE(18:1(9Z)/0:0)IsopentenyladenineTiagabinePhenylsulfateTauro-gamma-muricholic acidalpha-Hydroxyhippuric acidGly-Ala2-Hydroxybutyric acidcis-Aconitic acidArachidonic acid (AA)2-Linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolaminePelargonic acid2,2'-Iminodiacetic acid4-Hydroxyphenylacetic acid2-Arachidonoyl-1-stearoyl-sn-glycero-3-phosphoethanolamine3-Hydroxybutyric acid3-Hydroxyphenylacetic acidfalseSerum Metabolomics Reveals Stage-Dependent Metabolic Transitions During CIDR-PGF2alpha-Induced Estrus in Kazak EwesThis study investigated serum metabolic transitions during CIDR-PGF2alpha-induced estrus synchronization in Kazak ewes using UHPLC-MS/MS untargeted metabolomics. Eighteen Kazak ewes were assigned to three reproductive stages: luteal phase (LP), luteolysis phase (LDP), and estrus phase (OES), with six animals per group. Serum samples were analyzed by UHPLC-MS/MS on an Orbitrap Exploris 120 instrument using HILIC chromatography (BEH Amide column) in both positive and negative ionization modes. A total of 1,861 metabolites were annotated. Key biomarkers identified include choline, betaine aldehyde, sphingosine, 3-hydroxybutyrylcarnitine, and pregnanediol 3-O-glucuronide, reflecting stage-dependent shifts in phospholipid metabolism, energy utilization, and steroid hormone pathways. This study was funded by NSFC Grant No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