Data acquisitions were performed using Analyst 1.6.3 software (Sciex).
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["The sample extracts were analyzed using an UPLC-APCI-MS/MS system. The analytical conditions were as follow, LC: column, YMC C30(3 μm, 100 mm×2.0 mm i.d); solvent system, methanol acetonitrile (1:3, v/v) with 0.01% BHT and 0.1% formic acid (A), methyl tert-butyl ether with 0.01% BHT (B); gradient program, started at 0% B (0-3 min), increased to 70% B (3-5 min), then increased to 95% B (5-9 min), finally ramped back to 0% B (10-11 min); flow rate, 0.8 mL/min; temperature, 28°C; injection volume: 2 μL.
"],"publication":["TrcrtB regulates carotenoids biosynthesis, stress tolerance, conidiation and pathogenicity in the postharvest pink rot fungus, Trichothecium roseum."],"submitter_affiliation":["Henan Normal University"],"submitter_name":["Juncong Liu"],"organism_part":["mycelium"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The sample was freeze-dried, ground into powder (30 Hz, 1.5 min), and stored at -80°C until needed. 50 mg powder was weighted and extracted with 0.5 mL mixed solution of n-hexane: acetone: ethanol
(1:1:1, v/v/v). The extract was vortexed for 10 min at room temperature and sonicated for 5 min in ice water bath. The supernatants were collected after centrifuged at 12000 r/min for 5 min at 4°C. The residue
was re-extracted by repeating the above steps again under the same conditions. And then evaporated to dryness, reconstituted in 150μL dichloromethane. The solution was filtered through a 0.22 μm membrane filter for further LC-MS/MS analysis
"],"organism":["Trichothecium roseum"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15120"],"author":["Liu Juncong. Henan Normal University. 15237385613@163.com."],"data_transformation_protocol":["Carotenoids were analyzed using scheduled multiple reaction monitoring
"],"study_factor":["Treatment"],"submitter_email":["15237385613@163.com"],"sample_collection_protocol":["The spore suspensions (200 μ L, 1×10^6 CFU mL-1) of WT and ΔTrcrtB strains were evenly spread on PDA plates covered with cellophane and incubated at 28°C for 5 days. Then, fungal hyphae were collected and preserved in liquid nitrogen, immediately.
"],"omics_type":["Metabolomics"],"study_design":["mycelium","ultra-performance liquid chromatography-mass spectrometry","metabolite profiling assay","Carotenoids","SCIEX ExionLC AD","QTRAP 6500+","Trichothecium roseum","targeted metabolite profiling","experimental sample"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","mycelium","metabolite profiling assay","Carotenoids","SCIEX ExionLC AD","QTRAP 6500+","Trichothecium roseum","targeted metabolite profiling","experimental sample"],"mass_spectrometry_protocol":["Linear ion trap (LIT) and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP), QTRAP 6500+ LC-MS/MS System, equipped with an APCI Heated Nebulizer, operating in positive ion mode and controlled by Analyst 1.6.3 software (Sciex). The APCI source operation parameters were as follows: ion source, APCI+; source temperature 350°C; CUR were set at 25.0 psi. m/z range: 50-1250.
"],"metabolite_name":["rubixanthin-laurate","Apocarotenal","zeaxanthin-palmitate","zeaxanthin-caprate-laurate","5,6epoxy-luttein-dilaurate","Astaxanthin","lutein-laurate","¦Å-Carotene","Phytofluene","rubixanthin-caprate","violaxanthin-dilaurate","lutein-dilaurate","zeaxanthin-myristate-palmitate","echinenone","lutein-dimyristate","Lutein","violaxanthin-dibutyrate","zeaxanthin-oleate-palmitate","neochrome-palmitate","violaxanthin-myristate-laurate","Lycopene","¦Â-cryptoxanthin-oleate","¦Á-Cryptoxanthin","zeaxanthin-palmitate-stearate","antheraxanthin-dipalmitate","violaxanthin-dioleate","¦Â-cryptoxanthin-laurate","zeaxanthin-myristoleate","violaxanthin-myristate","zeaxanthin-dipalmitate_2","zeaxanthin-dilaurate","zeaxanthin-dimyristate","lutein-palmitate","Phytoene-1","rubixanthin-palmitate","Fucoxanthin_1","alloxanthin","Zeaxanthin","rubixanthin-myristate","violaxanthin-palmitate","violaxanthin-myristate-oleate","Antheraxanthin","¦Â-Cryptoxanthin","¦Ã-Carotene","zeaxanthin-laurate-myristate","¦Â-cryptoxanthin-palmitate","canthaxanthin","¦Á-Carotene","Violaxanthin_2","Capsanthin","Adonirubin","violaxanthin-dipalmitate","lutein-dioleate","¦Â-citraurin","violaxanthin-laurate","Capsorubin","lutein-stearate","violaxanthin-palmitoleate","lutein-dipalmitate","violaxanthin-dimyristate","5,6epoxy-lutein-caprate-palmitate","lutein-oleate","lutein-caprate","¦Â-Carotene","lutein-distearate","¦Â-cryptoxanthin-myristate","zeaxanthin-laurate-palmitate","violaxanthin-myristate-palmitate","lutein-myristate","Peridinin","Neoxanthin","violaxanthin-myristate-caprate"],"additional_accession":[]},"is_claimable":false,"name":"TrcrtB regulates carotenoids biosynthesis, stress tolerance, conidiation and pathogenicity in the postharvest pink rot fungus, Trichothecium roseum","description":"Trichothecium roseum is a highly destructive postharvest pathogenic fungus that causes pink mold rot in various fruits and leads to significant agricultural and economic losses. While Phytoene is known to be crucial for phytopathogens, its roles in postharvest fungi, remain largely obscure. In this study, we evaluated the functions of TrcrtB, a phytoene synthase, in T. roseum via in vivo and in vitro assays. Our results showed that knocked out of TrcrtB inhibited phytoene and its relevant downstream metabolites, such as lycopene, carotenes and carotenal, resulting in colorless colony and branching at the mycelial edges in the knockout mutant ΔTrcrtB. Compared with the wild type (WT) strain, the colony expansion was significantly reduced 35% and 19% at 5 and 7days post-inoculation (dpi), respectively, and conidiation was notably decreased to 45%, 36%, and 70% at 3, 5, and 7 dpi in ΔTrcrtB in vitro. Scanning electron microscope observation revealed similar results. Moreover, the ΔTrcrtB showed higher sensitivity to abiotic stresses that WT, as evidenced by inhibition rates of ΔTrcrtB colony expansion up to 71.76% (menadione), 47.73% (Congo red), 23.01% (SDS), and 17.13% (KCl). The pathogenicity of ΔTrcrtB was dramatically impaired by decreasing the rotten area up to 85.26% on apple fruit and 70.30% on pears fruit. These results suggest that TrcrtB and phytoene are critical for development, stress tolerance and pathogenicity of T. roseum. Collectively, our study highlights the roles of TrcrtB and phytoene in the pathogenic fungus T. roseum and crtB was suggested as a potential target for postharvest disease control.
","dates":{"publication":"2026-07-22","submission":"2026-07-22"},"accession":"MTBLS15120","cross_references":{}}