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and MS/MS spectra were matched against HMDB, METLIN, KEGG, and the Majorbio self-compiled database (MJDB). Internal-standard peaks and known false-positive features, including noise and column-bleed signals, were removed, and redundant features were pooled. Metabolite annotations were reported with metabolite name, mass-to-charge ratio, retention time, molecular formula, database identifier, and identification confidence."],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - reversed-phase","Liquid Chromatography MS - negative - reversed-phase"],"chromatography_protocol":["Reversed-phase liquid chromatography was performed using a Thermo Scientific UHPLC system coupled to an Orbitrap Exploris 240 mass spectrometer and an ACQUITY UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 micrometre; Waters). The flow rate was 0.40 mL/min and the column temperature was 40 degrees C."],"publication":["Time-Resolved Microbiome–Metabolome Remodeling Reveals Biotransformation Patterns in Liquid-Fermented Dendrobium officinale Flowers."],"submitter_name":["Mingyang XUAN"],"submitter_affiliation":["Hefei University of Technology"],"organism_part":["flower"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Fermentation supernatants were extracted using acetonitrile:methanol (1:1, v/v) containing four internal standards, including L-2-chlorophenylalanine (0.02 mg/mL). Samples were vortexed, sonicated at 5 degrees C, incubated at -20 degrees C, centrifuged at 13000 x g, dried under nitrogen, reconstituted in acetonitrile:water (1:1, v/v), sonicated, and centrifuged before LC-MS/MS analysis."],"organism":["Dendrobium officinale"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS15132"],"author":["Mingyang XUAN. Hefei University of Technology. mxuanab@connect.ust.hk."],"data_transformation_protocol":["LC-MS raw data were processed using Progenesis QI for baseline filtering, peak detection, integration, retention-time correction, and peak alignment. Features detected in at least 80% of samples in any group were retained. Missing values were imputed with the minimum value, peak intensities were sum-normalized, variables with QC RSD greater than 30% were excluded, and data were log10-transformed."],"study_factor":["Fermentation time"],"submitter_email":["mxuanab@connect.ust.hk"],"sample_collection_protocol":["Fermentation supernatants were collected at days 10, 20, 30, 40, 50, and 60 during a 60-day mixed-culture liquid fermentation of Dendrobium officinale flowers. Six biological replicates were collected at each time point. Supernatants were separated from fermentation pellets by centrifugation and stored at -20 degrees C until untargeted LC-MS/MS analysis. Four pooled quality-control samples were included for analytical quality control."],"omics_type":["Metabolomics"],"study_design":["pooled quality control sample","Metabolomics","untargeted metabolomics","mixed-culture fermentation","untargeted analysis","Thermo Scientific UHPLC system coupled to an Orbitrap Exploris 240 mass spectrometer","Thermo Scientific Orbitrap Exploris 240 mass spectrometer","cell culture supernatant","Dendrobium officinale","time series design","flower"],"curator_keywords":["pooled quality control sample","Metabolomics","untargeted metabolomics","mixed-culture fermentation","untargeted analysis","Thermo Scientific UHPLC system coupled to an Orbitrap Exploris 240 mass spectrometer","Thermo Scientific Orbitrap Exploris 240 mass spectrometer","cell culture supernatant","Dendrobium officinale","time series design","flower"],"mass_spectrometry_protocol":["Mass spectrometry data were acquired on a Thermo Scientific Orbitrap Exploris 240 mass spectrometer equipped with an electrospray ionization source in separate positive and negative ion modes. The mass scan range was m/z 70-1050. Data-dependent MS/MS acquisition used stepped normalized collision energies of 20, 40, and 60."],"metabolite_name":["Difloxacin","5-Formiminotetrahydrofolic Acid","Robinetin","N-[(3-Chlorophenyl)Methyl]-2-(5-Quinolin-6-Yl-1,3,4-Oxadiazol-2-Yl)Acetamide","His-Ile-Ile","Apigenin 7-(6''-Malonylglucoside)","Edulisin I","Thymidine","Myricetin","2-[[(3-Cyanopyridin-2-Yl)Amino]Carbamoyl]Benzoic Acid","Gilteritinib","Chitobiose","Luteolon","Indole-3-Acetylglutamic Acid","Catechin 3-Glucuronide","Quercetin-3,4'-Di-O-Glucoside","Ser-Met-Ser","7-Methylinosine","Concanamycin","3H-Indole-3-Propanoic Acid,A-Amino-","Pc(22:6/0:0)","Demethylsuberosin","Methylprednisolone Acetate","Pindone","Deoxyadenosine","Annomuricatin A","Myricanene A 5-[Arabinosyl-(1->6)-Glucoside]","Acuminoside","6-Epi-7-Isocucurbic Acid Glucoside","Asp-Phe-Ile","Ergothioneine","Thr-Val","Axillarin","Lpc(18:1)","Biotin Sulfone","N-(2,4-Dimethoxyphenyl)-2,5-Dimethyl-1,3-Oxazole-4-Carboxamide","Taxiresinol","Pyropheophytin B","Kaempferol-3-O-Rhamnoside-7-O-Rhamnoside","Noreugenin","N-[(2-Chlorophenyl)Methyl]-1-(3-Ethoxyquinoxalin-2-Yl)Piperidine-3-Carboxamide","Ramontoside","Glucoheptonic Acid","Norhydromorphone","1-Beta-D-Arabinofuranosyl-5-Fluorocytosine","3-Indolehydracrylic Acid","Penciclovir","4-[5-(1-Hydroxyethyl)Furan-2-Yl]-4-Oxobutanoic Acid","6,8-Dihydroxy-7-Methoxy-3-Methyl-3,4-Dihydroisochromen-1-One","Gln Leu Leu","Tetrahydrocoptisine","3-Deoxyguanosine","Diplosporin","Cichorioside K","Paeonimetabolin I","N-[2-(1H-Indol-3-Yl)Ethyl]Formamide","Cay10589","9-(2,5-Dimethoxyphenyl)Sulfonyl-1-Oxa-3,9-Diazaspiro[4.6]Undecan-2-One","Oxypinnatanine","Telotristat","Minoxidil Glucuronide","Pa(5-Iso Pgf2Vi/2:0)","Spiraeoside","Eremosulphoxinolide A","Histidylleucine"],"additional_accession":[]},"is_claimable":false,"name":"Time-Resolved Microbiome–Metabolome Remodeling Reveals Biotransformation Patterns in Liquid-Fermented Dendrobium officinale Flowers","description":"This study investigated temporal metabolomic changes during a 60-day mixed-culture liquid fermentation of Dendrobium officinale flowers. Fermentation supernatants were collected at days 10, 20, 30, 40, 50, and 60, with six biological replicates at each time point. Untargeted LC-MS/MS analysis was performed in positive and negative electrospray ionization modes using a UHPLC-Orbitrap Exploris 240 system. The deposited dataset contains raw mass spectrometry files for 36 biological samples and four pooled quality-control samples and supports the analysis of time-dependent metabolite remodeling during fermentation.","dates":{"publication":"2026-07-22","submission":"2026-07-22"},"accession":"MTBLS15132","cross_references":{"HMDB":["HMDB0038614","HMDB0014685","HMDB0255052","HMDB0252642","HMDB0252686","HMDB0251689","HMDB0033999","HMDB0247713","HMDB0266684","HMDB0250201","HMDB0011635","HMDB0031367","HMDB0034843","HMDB0259154","HMDB0030703","HMDB0250467","HMDB0034060","HMDB0028880","HMDB0302318","HMDB0256840","HMDB0039964","HMDB0034435","HMDB0039214","HMDB0038535","HMDB0029348","HMDB0036618","HMDB0302720","HMDB0010384","HMDB0297674","HMDB0303981","HMDB0035489","HMDB0000840","HMDB0002639","HMDB0029529","HMDB0037551","HMDB0029379","HMDB0036632","HMDB0029463","HMDB0303159","HMDB0014911","HMDB0041658","HMDB0246319","HMDB0029219","HMDB0039373","HMDB0038765","HMDB0246843","HMDB0035588","HMDB0304749","HMDB0257687","HMDB0246756","HMDB0060812","HMDB0034299","HMDB0031954","HMDB0041514","HMDB0039953","HMDB0258911","HMDB0000707;PW_C000558","HMDB0247453","HMDB0252748","HMDB0062266","HMDB0000085;PW_C000058","HMDB0011670","HMDB0260262","HMDB0029074","HMDB0029782","HMDB0000273","HMDB0303562","HMDB0014444","HMDB0258751","HMDB0039351","HMDB0304330","HMDB0254740","HMDB0246793","HMDB0258897","HMDB0028889","HMDB0252717","HMDB0246269","HMDB0038929","HMDB0252766","HMDB0001534","HMDB0002755;LMPK12110001","HMDB0059765","HMDB0256560","HMDB0303181","HMDB0258794","HMDB0003556","HMDB0250429","HMDB0301863","HMDB0240434","HMDB0003045","HMDB0003950","HMDB0041532","HMDB0302057","HMDB0251259","HMDB0034248","HMDB0002815","HMDB0014004","HMDB0029347","HMDB0030680","HMDB0256079","HMDB0004818","HMDB0266530","HMDB0030594","HMDB0037932;HMDB0257588;LMPK12112184","HMDB0254661","HMDB0029403","HMDB0038665","HMDB0000101;PW_C000068"],"LIPID MAPS":["LMPK12111237","LMGP01050056"]}}